Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were eluci...Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were elucidated by quantum chemical calculations and spectroscopic methods.The biogenetic pathway for 1-3 is proposed.In the bioassay,glasesterterpenoid C exhibited DNA topoisomerase 1(Top1)inhibitory activity compared with the positive control,camptothecin.These findings represent the first examples of sesterterpenoids with a 7-cyclohexyldecahydronaphthalene carbon skeleton from the root of L.glauca.展开更多
The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal condition...The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal conditions.In the present article,compatible sequential solvent extractions were applied to N.glauca leaves to enable the generation of enriched extracts containing higher metabolite content comparing to direct leaf extracts.Typically,between 60 to 100 metabolite components were identified within the fractions.The occurrence of plant fatty acids,fatty acid alcohols,alkanes,sterols and terpenoids was detected by gas liquid chromatography-mass spectrometry(GC-MS)and metabolite identification was confirmed by comparison of physico-chemical properties displayed by available authentic standards.Collectively,co-products such waxes,oils,fermentable sugars,and terpenoids were all identified and quantified.The enriched fractions of N.glauca revealed a high level of readily extractable hydrocarbons,oils and high value co-products.In addition,the saccharification yield and cell wall composition analyses in the stems revealed the potential of the residue material as a promising lignocellulosic substrate for the production of fermentable sugars.In conclusion a multifractional cascade for valuable compounds/commodities has been development,that uses N.glauca biomass.These data have enabled the evaluation of N.glauca material as a potential feedstock for biorefining.展开更多
【目的】探寻不同生境下石灰岩山地优势种淡竹Phyllostachys glauca Mc Clure蔸根和鞭根根际与非根际土壤的养分供应特征与根际效应差异,为喀斯特生境植物的养分管理利用提供理论依据。【方法】根据石灰岩山地裸岩率的不同划分了连续土(...【目的】探寻不同生境下石灰岩山地优势种淡竹Phyllostachys glauca Mc Clure蔸根和鞭根根际与非根际土壤的养分供应特征与根际效应差异,为喀斯特生境植物的养分管理利用提供理论依据。【方法】根据石灰岩山地裸岩率的不同划分了连续土(CS)、半连续土(SCS)、零星土(SS)3种生境,对3种生境淡竹林设置样地调查,进行淡竹不同类型根系土壤取样,测定其养分含量及根际效应,分析不同生境下淡竹根系土壤养分含量及根际效应的变化。【结果】1)随着裸岩率的升高,淡竹根际与非根际土壤铵态氮、硝态氮以及有效磷含量整体呈上升趋势。其中,以SS生境淡竹蔸根非根际土的铵态氮和硝态氮最高,分别为(28.10±1.70)、(2.81±0.35)mg/kg;有效磷则以SCS生境鞭根的非根际土含量最高,为(5.97±0.23)mg/kg,不同生境类型土壤铵态氮含量差异显著(P<0.05)。2)淡竹铵态氮根际效应随裸岩率的升高呈现由正效应转变为负效应的趋势,分布范围为-6.56%~44.57%。硝态氮根际效应在3种生境中变化趋势与铵态氮相同,分布范围为-35.22%~1.33%。3)蔸根和鞭根土壤有效磷含量及根际效应没有显著差异。【结论】石灰岩山地淡竹蔸根和鞭根的根际效应无显著差异,但从不同生境淡竹根系铵态氮和硝态氮的根际效应来看,淡竹具有喜铵厌硝的特点。展开更多
Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those...Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.展开更多
基金supported by the National Natural Science Foundation of China(No.82104524)the Basic and Applied Research Program of Guangzhou City(No.2024A04J9954)。
文摘Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were elucidated by quantum chemical calculations and spectroscopic methods.The biogenetic pathway for 1-3 is proposed.In the bioassay,glasesterterpenoid C exhibited DNA topoisomerase 1(Top1)inhibitory activity compared with the positive control,camptothecin.These findings represent the first examples of sesterterpenoids with a 7-cyclohexyldecahydronaphthalene carbon skeleton from the root of L.glauca.
基金Open Access funding enabled and organized by Projekt DEALThe research from the MultiBioPro project leading to these results has received funding from the European Union’s Seventh Framework Programme for research,technological development and demonstration under grant agreement 311804Further funding from the BBSRC 21EBTA-Celfacto project is acknowledged by PDF.
文摘The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal conditions.In the present article,compatible sequential solvent extractions were applied to N.glauca leaves to enable the generation of enriched extracts containing higher metabolite content comparing to direct leaf extracts.Typically,between 60 to 100 metabolite components were identified within the fractions.The occurrence of plant fatty acids,fatty acid alcohols,alkanes,sterols and terpenoids was detected by gas liquid chromatography-mass spectrometry(GC-MS)and metabolite identification was confirmed by comparison of physico-chemical properties displayed by available authentic standards.Collectively,co-products such waxes,oils,fermentable sugars,and terpenoids were all identified and quantified.The enriched fractions of N.glauca revealed a high level of readily extractable hydrocarbons,oils and high value co-products.In addition,the saccharification yield and cell wall composition analyses in the stems revealed the potential of the residue material as a promising lignocellulosic substrate for the production of fermentable sugars.In conclusion a multifractional cascade for valuable compounds/commodities has been development,that uses N.glauca biomass.These data have enabled the evaluation of N.glauca material as a potential feedstock for biorefining.
文摘【目的】探寻不同生境下石灰岩山地优势种淡竹Phyllostachys glauca Mc Clure蔸根和鞭根根际与非根际土壤的养分供应特征与根际效应差异,为喀斯特生境植物的养分管理利用提供理论依据。【方法】根据石灰岩山地裸岩率的不同划分了连续土(CS)、半连续土(SCS)、零星土(SS)3种生境,对3种生境淡竹林设置样地调查,进行淡竹不同类型根系土壤取样,测定其养分含量及根际效应,分析不同生境下淡竹根系土壤养分含量及根际效应的变化。【结果】1)随着裸岩率的升高,淡竹根际与非根际土壤铵态氮、硝态氮以及有效磷含量整体呈上升趋势。其中,以SS生境淡竹蔸根非根际土的铵态氮和硝态氮最高,分别为(28.10±1.70)、(2.81±0.35)mg/kg;有效磷则以SCS生境鞭根的非根际土含量最高,为(5.97±0.23)mg/kg,不同生境类型土壤铵态氮含量差异显著(P<0.05)。2)淡竹铵态氮根际效应随裸岩率的升高呈现由正效应转变为负效应的趋势,分布范围为-6.56%~44.57%。硝态氮根际效应在3种生境中变化趋势与铵态氮相同,分布范围为-35.22%~1.33%。3)蔸根和鞭根土壤有效磷含量及根际效应没有显著差异。【结论】石灰岩山地淡竹蔸根和鞭根的根际效应无显著差异,但从不同生境淡竹根系铵态氮和硝态氮的根际效应来看,淡竹具有喜铵厌硝的特点。
基金funded by the Foundation from Education Department of Jiangxi Province (2007-149)the National Natural Science Foundation of China (30460020)the Cultivation Programs for Young Scientists of Jiangxi Province (2008DQ01500)
文摘Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.
