Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were eluci...Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were elucidated by quantum chemical calculations and spectroscopic methods.The biogenetic pathway for 1-3 is proposed.In the bioassay,glasesterterpenoid C exhibited DNA topoisomerase 1(Top1)inhibitory activity compared with the positive control,camptothecin.These findings represent the first examples of sesterterpenoids with a 7-cyclohexyldecahydronaphthalene carbon skeleton from the root of L.glauca.展开更多
The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal condition...The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal conditions.In the present article,compatible sequential solvent extractions were applied to N.glauca leaves to enable the generation of enriched extracts containing higher metabolite content comparing to direct leaf extracts.Typically,between 60 to 100 metabolite components were identified within the fractions.The occurrence of plant fatty acids,fatty acid alcohols,alkanes,sterols and terpenoids was detected by gas liquid chromatography-mass spectrometry(GC-MS)and metabolite identification was confirmed by comparison of physico-chemical properties displayed by available authentic standards.Collectively,co-products such waxes,oils,fermentable sugars,and terpenoids were all identified and quantified.The enriched fractions of N.glauca revealed a high level of readily extractable hydrocarbons,oils and high value co-products.In addition,the saccharification yield and cell wall composition analyses in the stems revealed the potential of the residue material as a promising lignocellulosic substrate for the production of fermentable sugars.In conclusion a multifractional cascade for valuable compounds/commodities has been development,that uses N.glauca biomass.These data have enabled the evaluation of N.glauca material as a potential feedstock for biorefining.展开更多
Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those...Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.展开更多
The seedling height and ground diameter of 42 half-sib families of biennial Cyclobalanopsis glauca were investigated and compared. The results showed that there were extremely significant differences between different...The seedling height and ground diameter of 42 half-sib families of biennial Cyclobalanopsis glauca were investigated and compared. The results showed that there were extremely significant differences between different families in the terms of seedling height and ground diameter. According to the seedling height and ground diameter, families 39, 27, 28 and 19 of C. glauca were superior to other families at the seedling stage.展开更多
Episodic deposition has been recognized as a major factor affecting the decomposition rate of detrital material in salt marshes. In this paper, three one-off burial treatments, no burial treatment(0 cm, NBT), current ...Episodic deposition has been recognized as a major factor affecting the decomposition rate of detrital material in salt marshes. In this paper, three one-off burial treatments, no burial treatment(0 cm, NBT), current burial treatment(10 cm, CBT) and strong burial treatment(20 cm, SBT), were designed in intertidal zone of the Yellow River Estuary to determine the potential influences of episodic deposition on nutrient(C, N) and heavy metal(Pb, Cr, Cu, Zn, Ni, Mn, Cd, V and Co) variations in decomposing litters of Suaeda glauca. Results showed that although various burial treatments showed no statistical difference in decomposition rate of S. glauca, the values generally followed the sequence of CBT(0.002 403/d) > SBT(0.002 195/d) > NBT(0.002 060/d). The nutrients and heavy metals in decomposing litters of the three burial treatments exhibited different variations except for N, Cu, Cr, Ni and Co. Except for Mn, no significant differences in C, N, Pb, Cr, Cu, Zn, Ni, V and Co concentrations occurred among the three treatments(P > 0.05). With increasing burial depth, Cr and Cd levels generally increased while Cu, Ni and Mn concentrations decreased. Although episodic deposition was generally favorable for C and N release from S. glauca, its influence on release was insignificant. In the three burial treatments, Pb, Cr, Zn, Ni, Mn, V and Co stocks in S. glauca generally evidenced the export of metals from litter to environment, and, with increasing burial depth, the export amounts increased greatly. The S. glauca were particular efficient in binding Cd and releasing Pb, Cr, Zn, Ni, Mn, V and Co, and, with increasing burial depth, stocks of Cu in decomposing litters generally shifted from release to accumulation. The experiment indicated that the potential eco-toxic risk of Pb, Cr, Zn, Ni, Mn, V and Co exposure would be serious as the strong burial episodes occurred in S. glauca marsh.展开更多
Endophytic bacteria are promising bacterial fertilizers to improve plant growth under adverse environment.For ecological remediation of coastal wetlands,it was necessary to investigate the effect and interaction of en...Endophytic bacteria are promising bacterial fertilizers to improve plant growth under adverse environment.For ecological remediation of coastal wetlands,it was necessary to investigate the effect and interaction of endophytes on halophytes under saline-alkali stress.In this study,an endophytic bacterium strain HK1 isolated from halophytes was selected to infect Suaeda glauca under pH(7 and 8)and salinity gradient(150,300 and 450mmolL^(-1)).Strain HK1 was identified as Pantoea ananatis and it had ability to fix nitrogen,dissolve inorganic phosphorus and produce indole-3-aceticacid(IAA).The results showed that strain HK1 could promote the growth of S.glauca seedings when the salinity was less than 300mmolL^(-1),in view of longer shoot length and heavier fresh weight.The infected plants could produce more proline to decrease the permeability of cells,which content increased by 26.2%–61.1%compared to the non-infected group.Moreover,the oxidative stress of infected plants was relieved with the malondialdehyde(MDA)content decreased by 16.8%–32.9%,and the peroxidase(POD)activity and catalase(CAT)activity increased by 100%–500%and 6.2%–71.4%,respectively.Statistical analysis revealed that increasing proline content and enhancing CAT and POD activities were the main pathways to alleviate saline-alkali stress by strain HK1 infection,and the latter might be more important.This study illustrated that endophytic bacteria could promote the growth of halophytes by regulation of osmotic substances and strengthening antioxidant activities.This finding would be helpful for the bioremediation of coastal soil.展开更多
基金supported by the National Natural Science Foundation of China(No.82104524)the Basic and Applied Research Program of Guangzhou City(No.2024A04J9954)。
文摘Three novel sesterterpenoids glasesterterpenoids A-C(1-3),featuring an unprecedented 7-cyclohexyldecahydronaphthalene carbon skeleton,were isolated from the root of Lindera glauca(L.glauca).Their structures were elucidated by quantum chemical calculations and spectroscopic methods.The biogenetic pathway for 1-3 is proposed.In the bioassay,glasesterterpenoid C exhibited DNA topoisomerase 1(Top1)inhibitory activity compared with the positive control,camptothecin.These findings represent the first examples of sesterterpenoids with a 7-cyclohexyldecahydronaphthalene carbon skeleton from the root of L.glauca.
基金Open Access funding enabled and organized by Projekt DEALThe research from the MultiBioPro project leading to these results has received funding from the European Union’s Seventh Framework Programme for research,technological development and demonstration under grant agreement 311804Further funding from the BBSRC 21EBTA-Celfacto project is acknowledged by PDF.
文摘The evaluation of plant-based feedstocks is an important aspect of biorefining.Nicotiana glauca is a solanaceous,non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal conditions.In the present article,compatible sequential solvent extractions were applied to N.glauca leaves to enable the generation of enriched extracts containing higher metabolite content comparing to direct leaf extracts.Typically,between 60 to 100 metabolite components were identified within the fractions.The occurrence of plant fatty acids,fatty acid alcohols,alkanes,sterols and terpenoids was detected by gas liquid chromatography-mass spectrometry(GC-MS)and metabolite identification was confirmed by comparison of physico-chemical properties displayed by available authentic standards.Collectively,co-products such waxes,oils,fermentable sugars,and terpenoids were all identified and quantified.The enriched fractions of N.glauca revealed a high level of readily extractable hydrocarbons,oils and high value co-products.In addition,the saccharification yield and cell wall composition analyses in the stems revealed the potential of the residue material as a promising lignocellulosic substrate for the production of fermentable sugars.In conclusion a multifractional cascade for valuable compounds/commodities has been development,that uses N.glauca biomass.These data have enabled the evaluation of N.glauca material as a potential feedstock for biorefining.
基金funded by the Foundation from Education Department of Jiangxi Province (2007-149)the National Natural Science Foundation of China (30460020)the Cultivation Programs for Young Scientists of Jiangxi Province (2008DQ01500)
文摘Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.
基金Supported by Special Scientific Research Project for Forestry Public Welfare Industry(201504301)
文摘The seedling height and ground diameter of 42 half-sib families of biennial Cyclobalanopsis glauca were investigated and compared. The results showed that there were extremely significant differences between different families in the terms of seedling height and ground diameter. According to the seedling height and ground diameter, families 39, 27, 28 and 19 of C. glauca were superior to other families at the seedling stage.
基金Under the auspices of National Natural Science Foundation of China(No.41971128,41371104)Key Foundation of Science and Technology Department of Fujian Province(No.2016R1032-1)the Award Program for Min River Scholar in Fujian Province(No.Min 201531)。
文摘Episodic deposition has been recognized as a major factor affecting the decomposition rate of detrital material in salt marshes. In this paper, three one-off burial treatments, no burial treatment(0 cm, NBT), current burial treatment(10 cm, CBT) and strong burial treatment(20 cm, SBT), were designed in intertidal zone of the Yellow River Estuary to determine the potential influences of episodic deposition on nutrient(C, N) and heavy metal(Pb, Cr, Cu, Zn, Ni, Mn, Cd, V and Co) variations in decomposing litters of Suaeda glauca. Results showed that although various burial treatments showed no statistical difference in decomposition rate of S. glauca, the values generally followed the sequence of CBT(0.002 403/d) > SBT(0.002 195/d) > NBT(0.002 060/d). The nutrients and heavy metals in decomposing litters of the three burial treatments exhibited different variations except for N, Cu, Cr, Ni and Co. Except for Mn, no significant differences in C, N, Pb, Cr, Cu, Zn, Ni, V and Co concentrations occurred among the three treatments(P > 0.05). With increasing burial depth, Cr and Cd levels generally increased while Cu, Ni and Mn concentrations decreased. Although episodic deposition was generally favorable for C and N release from S. glauca, its influence on release was insignificant. In the three burial treatments, Pb, Cr, Zn, Ni, Mn, V and Co stocks in S. glauca generally evidenced the export of metals from litter to environment, and, with increasing burial depth, the export amounts increased greatly. The S. glauca were particular efficient in binding Cd and releasing Pb, Cr, Zn, Ni, Mn, V and Co, and, with increasing burial depth, stocks of Cu in decomposing litters generally shifted from release to accumulation. The experiment indicated that the potential eco-toxic risk of Pb, Cr, Zn, Ni, Mn, V and Co exposure would be serious as the strong burial episodes occurred in S. glauca marsh.
基金supported by the Shandong Province’s Natural Science Foundation(No.ZR2019MD033).
文摘Endophytic bacteria are promising bacterial fertilizers to improve plant growth under adverse environment.For ecological remediation of coastal wetlands,it was necessary to investigate the effect and interaction of endophytes on halophytes under saline-alkali stress.In this study,an endophytic bacterium strain HK1 isolated from halophytes was selected to infect Suaeda glauca under pH(7 and 8)and salinity gradient(150,300 and 450mmolL^(-1)).Strain HK1 was identified as Pantoea ananatis and it had ability to fix nitrogen,dissolve inorganic phosphorus and produce indole-3-aceticacid(IAA).The results showed that strain HK1 could promote the growth of S.glauca seedings when the salinity was less than 300mmolL^(-1),in view of longer shoot length and heavier fresh weight.The infected plants could produce more proline to decrease the permeability of cells,which content increased by 26.2%–61.1%compared to the non-infected group.Moreover,the oxidative stress of infected plants was relieved with the malondialdehyde(MDA)content decreased by 16.8%–32.9%,and the peroxidase(POD)activity and catalase(CAT)activity increased by 100%–500%and 6.2%–71.4%,respectively.Statistical analysis revealed that increasing proline content and enhancing CAT and POD activities were the main pathways to alleviate saline-alkali stress by strain HK1 infection,and the latter might be more important.This study illustrated that endophytic bacteria could promote the growth of halophytes by regulation of osmotic substances and strengthening antioxidant activities.This finding would be helpful for the bioremediation of coastal soil.
