Eight sesquiterpenes were isolated from Ainsliaea glabra.Among them,a new guaianolide sesquiterpenoid,named ainsglaolide A(1),and seven known sesquiterpenoids 2~8 were identified.Compound 1 was a novel guaianolide ses...Eight sesquiterpenes were isolated from Ainsliaea glabra.Among them,a new guaianolide sesquiterpenoid,named ainsglaolide A(1),and seven known sesquiterpenoids 2~8 were identified.Compound 1 was a novel guaianolide sesquiterpenoid featuring a rare six-membered lactone ring formed between C-4 and C-15.The new compound structure was elucidated by HRESI-MS and NMR spectroscopy,as well as electrostatic circular dichroism(ECD)calculations were used to further confirm the absolute of 1.Anti-inflammatory activity tests were conducted on the separated compounds,indicating that compound 1 had significant inhibitory effect on NLRP3 inflammasome with an IC_(50) value of 1.86μmol/L.展开更多
To elucidate the mechanisms underlying the therapeutic effects of the herbal medicine pair Smilax Glabra and Semen Coicis in treating gout and hyperuricemia,a comprehensive analysis was conducted using network pharmac...To elucidate the mechanisms underlying the therapeutic effects of the herbal medicine pair Smilax Glabra and Semen Coicis in treating gout and hyperuricemia,a comprehensive analysis was conducted using network pharmacology and molecular docking methods.Disease-associated targets for gout and hyperuricemia were identified from the GeneCards,OMIM,Disgenet,and TTD databases,while the key active components and their corresponding targets for Smilax Glabra and Semen Coicis were obtained from the TCSMP database.The intersection of these targets enabled the construction of a protein-protein interaction(PPI)network,which was subsequently visualized and analyzed.Core targets were further subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses to elucidate the biological processes and pathways involved.Molecular docking was then employed to validate the reliability of the interactions between the active components and the identified targets.The analysis revealed that Smilax Glabra and Semen Coicis contained 15 bioactive components that interacted with 393 potential targets,while gout and hyperuricemia were associated with 660 targets in total.The primary active compounds implicated in treating these conditions included diosgenin,quercetin,and naringenin,which were found to interact with crucial hub targets such as BCL2,CASP3,and MAPK3.These interactions suggested that the herbal medicine pair modulated several biological processes,including gland development and the regulation of body fluid levels,through pathways involving membrane rafts,membrane microdomains,and nuclear receptor activities.Enrichment analyses highlighted their involvement in multiple signaling pathways,such as EGFR tyrosine kinase inhibitor resistance,phospholipase D signaling,and platelet activation.Molecular docking confirmed the strong binding affinities between the hub genes and the major active components,supporting their potential role in therapeutic efficacy.This study demonstrated that Smilax Glabra and Semen Coicis might offer a promising therapeutic strategy for gout and hyperuricemia by targeting multiple molecular components,biological functions,and pathways.The findings underscored the unique potential of traditional Chinese medicine(TCM)in managing complex diseases by leveraging synergistic effects across diverse biological mechanisms.展开更多
Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability...Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.展开更多
A novel phenylpropanoid-substituted catechin glycoside glabraoside A 1 and a new dihydrochalcone 3＇-（7＂-allylphenyl）-2＇,4＇,4＂-trihydroxy-6＇-methoxydihydrochalcone 2 were isolated from the herbs of Sarcandra gl...A novel phenylpropanoid-substituted catechin glycoside glabraoside A 1 and a new dihydrochalcone 3＇-（7＂-allylphenyl）-2＇,4＇,4＂-trihydroxy-6＇-methoxydihydrochalcone 2 were isolated from the herbs of Sarcandra glabra. Their structures were elucidated on the basis of spectroscopic analyses and chiroptical methods.展开更多
One new sesquiterpene lactone,1 α,8 α,9 α-trihydroxyeudesman-3（4）,7（11）-dien-8 β,12-olide（1） and two new phenylpropanoidsubstituted catechin glycosides,glabraoside C（2） and glabraoside D（3） were isolated...One new sesquiterpene lactone,1 α,8 α,9 α-trihydroxyeudesman-3（4）,7（11）-dien-8 β,12-olide（1） and two new phenylpropanoidsubstituted catechin glycosides,glabraoside C（2） and glabraoside D（3） were isolated from the whole plant of Sarcandra glabra. Their structures were established by the analyses of spectral and chemical evidences.展开更多
Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,w...Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material basis.Our findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis model.These improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and flaking.By transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular processes.Specifically,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase cells.Furthermore,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial cells.Of particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT cells.In summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg balance.These findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.展开更多
A new flavonol glycoside, named neosmitilbin was isolated form the rhizome of Smilax glabra. Its structure and absolute configuration were elucidated on the basis of spectroscopic studies.
Sarglanoids A-F,six new sesquiterpenoids belonging to eudesmane(1-5)and eremophilane(6)types,were isolated from the leaves of Sarcandra glabra,a famous traditional Chinese medicine(TCM).Their structures including abso...Sarglanoids A-F,six new sesquiterpenoids belonging to eudesmane(1-5)and eremophilane(6)types,were isolated from the leaves of Sarcandra glabra,a famous traditional Chinese medicine(TCM).Their structures including absolute configurations were elucidated through extensive spectroscopic analysis and electronic circular dichroism(ECD)calculations.Compounds 1-2 were rare N-containing eudesmane-type sesquiterpenoids.Compound 3 exhibited inhibitory activity against nitric oxide(NO)production in lipopolysaccharides(LPS)-induced RAW 264.7 cells with IC_(50) values at 20.00±1.30μmol·L^(-1).These findings provide scientific evidence for sesquiterpenoids as the material foundation of S.glabra.展开更多
Sarcandra glabra(Thunb.)Nakai is a perennial evergreen herb categorised within the Sarcandra Gardner genus under the Chloranthaceae family.Indigenous to tropical and subtropical regions of East Asia and India,this spe...Sarcandra glabra(Thunb.)Nakai is a perennial evergreen herb categorised within the Sarcandra Gardner genus under the Chloranthaceae family.Indigenous to tropical and subtropical regions of East Asia and India,this species is extensively distributed across China,particularly in the southern regions(Sichuan,Yunnan,and Jiangxi).In addition to its high ornamental value,S.glabra has a rich history of use in traditional Chinese medicine,evident through its empirical prescriptions for various ailments like pneumonia,dysentery,fractures,bruises,numbness,amenorrhea,rheumatism,and other diseases.Besides,modern pharmacological studies have revealed various biological activities,such as antitumour,anti-bacterial,anti-viral anti-inflammatory and immunomodulatory effects.The diverse chemical constituents of S.glabra have fascinated natural product researchers since the 1900s.To date,over 400 compounds including terpenoids,coumarins,lignans,flavonoids,sterols,anthraquinones,organic acids,and organic esters have been isolated and characterised,some featuring unprecedented structures.This review comprehensively examines the current understanding of S.glabra’s phytochemistry and pharmacology,with emphasis on the chemistry and biosynthesis of its unique chemotaxonomic marker,the lindenane-type sesquiterpenoids.展开更多
This study was conducted to comprehensively evaluate the effects of salicylic acid and sodium molybdate on cold tolerance of an ornamental plant Bougainvillea glabra and to provide a theoretical guidance for landscape...This study was conducted to comprehensively evaluate the effects of salicylic acid and sodium molybdate on cold tolerance of an ornamental plant Bougainvillea glabra and to provide a theoretical guidance for landscape maintenance.B.glabra plants were treated with 0.5 mmol/L salicylic acid and 2.0 μmol/L alone or in combination,and then exposed to low temperature stress before physiological indices were measured.The results showed that all salicylic acid and sodium molybdate treatments reduced the relative conductivity and malondialdehyde( MDA) content of B.glabra to varying extents under the stress of low temperature,and more significant effect was achieved by using the two agents in combination.Oxygen free radicals production rate increased with decreasing temperature from 20 to 6 ℃,but declined with temperature decreasing from 3 to-3 ℃.The SOD activity of the control( CK) was significantly lower than that of other treatments at 0 and-3 ℃.The treatments with salicylic acid and sodium molybdate alone and in combination increased POD activity of B.glabra plants,especially at 0 ℃,as the POD activity of treatments T1,T2 and T3 was significantly higher than that of CK at 0 ℃.In addition,under low temperature stress,the contents of soluble sugar,starch and proline increased initially and decreased subsequently with temperature decreasing.The soluble sugar content at 3 ℃,starch and proline contents at 0 and-3 ℃ in treatments with salicylic acid and sodium molybdate alone and in combination were significantly higher than those of CK.All above results proved that salicylic acid and sodium molybdate are able to improve cold tolerance of B.glabra,and better effect can be achieved by using them together.展开更多
To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical c...To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical constituents and distribution characteristics of roots,stems and leaves in both wild and cultivated S.glabra were investigated through UHPLC-TOF-MS method.The result revealed that there were significant differences between roots,stems and leaves in S.glabra.And the chemical contents in the root part were less or even absence than those in leaf and stem,which suggested the root organ could be excluded as medicine.Meanwhile,the chemical contents of stems and leaves in cultivated S.glabra was sightly higher than that of wild samples.Therefore,cultivated S.glabra may have a high potential for substitution of wild S.glabra without affecting its pharmaceutical properties.In summary,our study could provide important information to the molecular basis for quality control of S.glabra.展开更多
Microwave-assisted extraction(MAE)was used for extraction of effective components of sarcandra glabra(Thunb.),and then chromatographic fingerprint of sarcandra glabra(Thunb.)was studied by high performance liquid chro...Microwave-assisted extraction(MAE)was used for extraction of effective components of sarcandra glabra(Thunb.),and then chromatographic fingerprint of sarcandra glabra(Thunb.)was studied by high performance liquid chromatography/diode array detector(HPLC/DAD).The conditions of MAE were optimized by an orthogonal experiment,and then the authentication and validation of the chromatographic fingerprint were conducted.Nine peaks were identified as common peaks in the fingerprint chromatograms,and isofraxidin was considered as a reference compound and quantified.Relative standard deviations of retention time and peak area of each component were less than 3%and 8%,respectively.Similarity and difference analysis were conducted by use of PCA and relation coefficient.Twenty batches of sarcandra glabra(Thunb.)samples from two different producing areas could be classified into two different groups in the PCA model.The results showed that MAE-HPLC/DAD method was simple,efficient and stable for the study of complex chromatographic fingerprint of sarcandra glabra(Thunb.),which could provide more reliable and precise information for quality evaluation.展开更多
A novel morphine alkaloid, named gindarudine 1 has been isolated from ethanol extract of Stephania glabra tubers, together with four known alkaloids, palmatine, dehydrocorydalmine, stepharanine, and 8-（4＇-methoxyben...A novel morphine alkaloid, named gindarudine 1 has been isolated from ethanol extract of Stephania glabra tubers, together with four known alkaloids, palmatine, dehydrocorydalmine, stepharanine, and 8-（4＇-methoxybenzyl）-xylopinine. Compound 1 was elucidated as 3,6-O,N-detrimethyl-10-hydroxy-l-methoxy-thebaine by means of spectroscopic data including 2D NMR studies. C 2009 Deepak Kumar Semwal. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
Sarcandra glabra(Thunb.)Nakai.,belonging to Chloranthaceae genus of Sarcandra family,is widely used as the common Chinese herbal medicine in folk.As a traditional medicinal agent,a wide spectrum of chemical constituen...Sarcandra glabra(Thunb.)Nakai.,belonging to Chloranthaceae genus of Sarcandra family,is widely used as the common Chinese herbal medicine in folk.As a traditional medicinal agent,a wide spectrum of chemical constituents have been isolated from the S.glabra,including sesquiterpenoids,triperpenoids,flavonoids,coumarins,phenolic acids and volatile oil.Modern pharmacological studies have shown that S.glabra has many biological activities such as anti-cancer,hepatoprotective activity,anti-inflammatory and anti-asthmatic activities.For the further development of this plant,the primary chemical constitutions of S.glabra and their activities have been summarized in this study.展开更多
An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. T...An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile：2% acetic acid in water （75：25, v/v） with a flow rate of 200 μL/min. The qTOF-MS was operated under multiple reaction monitoring （MRM） mode using the electrospray ionization （ESI） technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction （ASE）, extraction under ultrasonic waves （USW） and the classical extraction by percolation （CE） method was done and quantification of these extracts was also carried out by the proposed method.展开更多
文摘Eight sesquiterpenes were isolated from Ainsliaea glabra.Among them,a new guaianolide sesquiterpenoid,named ainsglaolide A(1),and seven known sesquiterpenoids 2~8 were identified.Compound 1 was a novel guaianolide sesquiterpenoid featuring a rare six-membered lactone ring formed between C-4 and C-15.The new compound structure was elucidated by HRESI-MS and NMR spectroscopy,as well as electrostatic circular dichroism(ECD)calculations were used to further confirm the absolute of 1.Anti-inflammatory activity tests were conducted on the separated compounds,indicating that compound 1 had significant inhibitory effect on NLRP3 inflammasome with an IC_(50) value of 1.86μmol/L.
文摘To elucidate the mechanisms underlying the therapeutic effects of the herbal medicine pair Smilax Glabra and Semen Coicis in treating gout and hyperuricemia,a comprehensive analysis was conducted using network pharmacology and molecular docking methods.Disease-associated targets for gout and hyperuricemia were identified from the GeneCards,OMIM,Disgenet,and TTD databases,while the key active components and their corresponding targets for Smilax Glabra and Semen Coicis were obtained from the TCSMP database.The intersection of these targets enabled the construction of a protein-protein interaction(PPI)network,which was subsequently visualized and analyzed.Core targets were further subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses to elucidate the biological processes and pathways involved.Molecular docking was then employed to validate the reliability of the interactions between the active components and the identified targets.The analysis revealed that Smilax Glabra and Semen Coicis contained 15 bioactive components that interacted with 393 potential targets,while gout and hyperuricemia were associated with 660 targets in total.The primary active compounds implicated in treating these conditions included diosgenin,quercetin,and naringenin,which were found to interact with crucial hub targets such as BCL2,CASP3,and MAPK3.These interactions suggested that the herbal medicine pair modulated several biological processes,including gland development and the regulation of body fluid levels,through pathways involving membrane rafts,membrane microdomains,and nuclear receptor activities.Enrichment analyses highlighted their involvement in multiple signaling pathways,such as EGFR tyrosine kinase inhibitor resistance,phospholipase D signaling,and platelet activation.Molecular docking confirmed the strong binding affinities between the hub genes and the major active components,supporting their potential role in therapeutic efficacy.This study demonstrated that Smilax Glabra and Semen Coicis might offer a promising therapeutic strategy for gout and hyperuricemia by targeting multiple molecular components,biological functions,and pathways.The findings underscored the unique potential of traditional Chinese medicine(TCM)in managing complex diseases by leveraging synergistic effects across diverse biological mechanisms.
文摘Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.
基金the National Natural Science Foundation of China （No. 20432030） for financial support.
文摘A novel phenylpropanoid-substituted catechin glycoside glabraoside A 1 and a new dihydrochalcone 3＇-（7＂-allylphenyl）-2＇,4＇,4＂-trihydroxy-6＇-methoxydihydrochalcone 2 were isolated from the herbs of Sarcandra glabra. Their structures were elucidated on the basis of spectroscopic analyses and chiroptical methods.
基金supported by the National Natural Science Foundation of China(Nos.20772157 and 21132009)National Science and Technology Project of China(No.2011ZX09307-002-01)the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT1007)
文摘One new sesquiterpene lactone,1 α,8 α,9 α-trihydroxyeudesman-3（4）,7（11）-dien-8 β,12-olide（1） and two new phenylpropanoidsubstituted catechin glycosides,glabraoside C（2） and glabraoside D（3） were isolated from the whole plant of Sarcandra glabra. Their structures were established by the analyses of spectral and chemical evidences.
基金supported by the National Natural Science Foundation of China(Nos.82074114,81973562 and 81773995),the Scholar of the 14th batch of“Six Talents Peak”highlevel talent selection(No.SWYY-094)the“Double First-Class”University project(No.CPU2018GY33).
文摘Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material basis.Our findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis model.These improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and flaking.By transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular processes.Specifically,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase cells.Furthermore,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial cells.Of particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT cells.In summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg balance.These findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.
文摘A new flavonol glycoside, named neosmitilbin was isolated form the rhizome of Smilax glabra. Its structure and absolute configuration were elucidated on the basis of spectroscopic studies.
基金supported in part by the National Natural Science Foundation of China(No.320703S9)the 111 Project from Ministry of Education of China and the State Administration of Foreign Export Affairs of China(No.B18056)the Drug Innovation Major Project(No.2018ZX09735002-003).
文摘Sarglanoids A-F,six new sesquiterpenoids belonging to eudesmane(1-5)and eremophilane(6)types,were isolated from the leaves of Sarcandra glabra,a famous traditional Chinese medicine(TCM).Their structures including absolute configurations were elucidated through extensive spectroscopic analysis and electronic circular dichroism(ECD)calculations.Compounds 1-2 were rare N-containing eudesmane-type sesquiterpenoids.Compound 3 exhibited inhibitory activity against nitric oxide(NO)production in lipopolysaccharides(LPS)-induced RAW 264.7 cells with IC_(50) values at 20.00±1.30μmol·L^(-1).These findings provide scientific evidence for sesquiterpenoids as the material foundation of S.glabra.
文摘Sarcandra glabra(Thunb.)Nakai is a perennial evergreen herb categorised within the Sarcandra Gardner genus under the Chloranthaceae family.Indigenous to tropical and subtropical regions of East Asia and India,this species is extensively distributed across China,particularly in the southern regions(Sichuan,Yunnan,and Jiangxi).In addition to its high ornamental value,S.glabra has a rich history of use in traditional Chinese medicine,evident through its empirical prescriptions for various ailments like pneumonia,dysentery,fractures,bruises,numbness,amenorrhea,rheumatism,and other diseases.Besides,modern pharmacological studies have revealed various biological activities,such as antitumour,anti-bacterial,anti-viral anti-inflammatory and immunomodulatory effects.The diverse chemical constituents of S.glabra have fascinated natural product researchers since the 1900s.To date,over 400 compounds including terpenoids,coumarins,lignans,flavonoids,sterols,anthraquinones,organic acids,and organic esters have been isolated and characterised,some featuring unprecedented structures.This review comprehensively examines the current understanding of S.glabra’s phytochemistry and pharmacology,with emphasis on the chemistry and biosynthesis of its unique chemotaxonomic marker,the lindenane-type sesquiterpenoids.
基金Supported by Applied Basic Research Project of Yunnan Province(2017FD087)National Natural Science Foundation of China(31660559)
文摘This study was conducted to comprehensively evaluate the effects of salicylic acid and sodium molybdate on cold tolerance of an ornamental plant Bougainvillea glabra and to provide a theoretical guidance for landscape maintenance.B.glabra plants were treated with 0.5 mmol/L salicylic acid and 2.0 μmol/L alone or in combination,and then exposed to low temperature stress before physiological indices were measured.The results showed that all salicylic acid and sodium molybdate treatments reduced the relative conductivity and malondialdehyde( MDA) content of B.glabra to varying extents under the stress of low temperature,and more significant effect was achieved by using the two agents in combination.Oxygen free radicals production rate increased with decreasing temperature from 20 to 6 ℃,but declined with temperature decreasing from 3 to-3 ℃.The SOD activity of the control( CK) was significantly lower than that of other treatments at 0 and-3 ℃.The treatments with salicylic acid and sodium molybdate alone and in combination increased POD activity of B.glabra plants,especially at 0 ℃,as the POD activity of treatments T1,T2 and T3 was significantly higher than that of CK at 0 ℃.In addition,under low temperature stress,the contents of soluble sugar,starch and proline increased initially and decreased subsequently with temperature decreasing.The soluble sugar content at 3 ℃,starch and proline contents at 0 and-3 ℃ in treatments with salicylic acid and sodium molybdate alone and in combination were significantly higher than those of CK.All above results proved that salicylic acid and sodium molybdate are able to improve cold tolerance of B.glabra,and better effect can be achieved by using them together.
基金the Science and Technology Development Fund,Macao SAR(No.0023/2019/AKP)Guangxi Science and Technology Department Fund(No.AD17195002)。
文摘To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical constituents and distribution characteristics of roots,stems and leaves in both wild and cultivated S.glabra were investigated through UHPLC-TOF-MS method.The result revealed that there were significant differences between roots,stems and leaves in S.glabra.And the chemical contents in the root part were less or even absence than those in leaf and stem,which suggested the root organ could be excluded as medicine.Meanwhile,the chemical contents of stems and leaves in cultivated S.glabra was sightly higher than that of wild samples.Therefore,cultivated S.glabra may have a high potential for substitution of wild S.glabra without affecting its pharmaceutical properties.In summary,our study could provide important information to the molecular basis for quality control of S.glabra.
基金supported the National Natural Science Foundation of China(Nos.20375050 and 20905080)National Key Technologies R&D Program of the 11th-five-year Plan(No.2006BAK03A08)Guangdong Provincial Department of Science and Technology(No.2009B010900021)
文摘Microwave-assisted extraction(MAE)was used for extraction of effective components of sarcandra glabra(Thunb.),and then chromatographic fingerprint of sarcandra glabra(Thunb.)was studied by high performance liquid chromatography/diode array detector(HPLC/DAD).The conditions of MAE were optimized by an orthogonal experiment,and then the authentication and validation of the chromatographic fingerprint were conducted.Nine peaks were identified as common peaks in the fingerprint chromatograms,and isofraxidin was considered as a reference compound and quantified.Relative standard deviations of retention time and peak area of each component were less than 3%and 8%,respectively.Similarity and difference analysis were conducted by use of PCA and relation coefficient.Twenty batches of sarcandra glabra(Thunb.)samples from two different producing areas could be classified into two different groups in the PCA model.The results showed that MAE-HPLC/DAD method was simple,efficient and stable for the study of complex chromatographic fingerprint of sarcandra glabra(Thunb.),which could provide more reliable and precise information for quality evaluation.
文摘A novel morphine alkaloid, named gindarudine 1 has been isolated from ethanol extract of Stephania glabra tubers, together with four known alkaloids, palmatine, dehydrocorydalmine, stepharanine, and 8-（4＇-methoxybenzyl）-xylopinine. Compound 1 was elucidated as 3,6-O,N-detrimethyl-10-hydroxy-l-methoxy-thebaine by means of spectroscopic data including 2D NMR studies. C 2009 Deepak Kumar Semwal. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
文摘Sarcandra glabra(Thunb.)Nakai.,belonging to Chloranthaceae genus of Sarcandra family,is widely used as the common Chinese herbal medicine in folk.As a traditional medicinal agent,a wide spectrum of chemical constituents have been isolated from the S.glabra,including sesquiterpenoids,triperpenoids,flavonoids,coumarins,phenolic acids and volatile oil.Modern pharmacological studies have shown that S.glabra has many biological activities such as anti-cancer,hepatoprotective activity,anti-inflammatory and anti-asthmatic activities.For the further development of this plant,the primary chemical constitutions of S.glabra and their activities have been summarized in this study.
文摘An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry （UPLC-qTOF-MS/MS） method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile：2% acetic acid in water （75：25, v/v） with a flow rate of 200 μL/min. The qTOF-MS was operated under multiple reaction monitoring （MRM） mode using the electrospray ionization （ESI） technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction （ASE）, extraction under ultrasonic waves （USW） and the classical extraction by percolation （CE） method was done and quantification of these extracts was also carried out by the proposed method.
