The objective of this research is to lift the dormancy of seeds of S. kunthianum in with a view to domesticating them. In this experiment, we used the soaking of seeds in sulfuric acid for different durations (10 min,...The objective of this research is to lift the dormancy of seeds of S. kunthianum in with a view to domesticating them. In this experiment, we used the soaking of seeds in sulfuric acid for different durations (10 min, 30 min and 1 h). The treated seeds were sown in transparent germinators. The explants obtained were then transplanted into pots containing five types of substrate (black earth, fine sand, sawdust, mixtures 1/1 of sawdust/black earth and 1/1 of sawdust/fine sand). This second test made it possible to determine the rate of germination on the substrate. The results obtained compared to those of the control showed a clear reduction in the latency time or germination time (62 hours) instead of 6 days (control), in the germination time 3 days/7 days (control) and a very high germination rate (100%). At the 5% threshold, soaking for 10 min and 30 min (100%) is the best result on the germination of S. kunthianum seeds. Soaking for 1 hour gives (7%) very low. After transplantation of the explants, the percentages obtained independently of the treatments show that the 1/1 mixtures of sawdust/black earth (75.19%), black earth (73.33%) and sawdust/fine sand (66.30%) have a higher performance than the others. The type of germination is epigeal. This work makes it possible to domesticate S. kunthianum in Chad.展开更多
Aconitum heterophyllum Wall.ex Royle,an endangered Ayurvedic medicinal plant,is predominantly found in the alpine and subalpine zones of the Himalayas.This study aimed to enhance seed germination under various soil co...Aconitum heterophyllum Wall.ex Royle,an endangered Ayurvedic medicinal plant,is predominantly found in the alpine and subalpine zones of the Himalayas.This study aimed to enhance seed germination under various soil conditions,to facilitate conservation and provide opportunities for income generation through large-scale cultivation.The selection of soil treatments was based on their contrasting physicochemical properties and known influence on seed germination in related high altitude species.The study was conducted in a mist chamber at the Deoban nursery in Dehradun,India,using five soil conditions:normal forest soil(NS),vermiculite(Vc),vermiculite+normal forest soil(Vc+NS),normal forest soil+sand(NS+S),and a mixture of soil,sand,vermiculite,and vermicompost(NS+S+Vc+VCo).All the soil treatments were replicated ten times under the controlled environmental conditions.These substrates were selected to represent a gradient of water retention,aeration,nutrient content,and organic matter composition,which are known to affect seed germination and early seedling development.Results showed significant different among treatments in terms of physical and chemical soil properties in sand,silt,clay,pH,organic carbon,organic matter,total nitrogen,total phosphorus,as well as growth parameters such as,seed vigor index,root length,plant length,and leaf number across soil conditions.Among all treatments,vermiculite alone showed the highest germination rate(73.33%),peak germination value(0.719),and seed vigor index(24.02),along with superior growth parameters and the lowest seed mortality index(2.45).These findings in addition to its low seed mortality underscore vermiculite's potential as a suitable growth medium for the ex-situ cultivation of A.heterophyllum offering a promising alternative to wild harvesting while supporting conservation and sustainable use of this valuable Himalayan species.展开更多
Seed vigor is critical for uniform germination and emergence,directly influencing subsequent seedling development.This is especially important under both normal and stress conditions that may arise post-sowing.Chillin...Seed vigor is critical for uniform germination and emergence,directly influencing subsequent seedling development.This is especially important under both normal and stress conditions that may arise post-sowing.Chilling stress during emergence and early growth poses significant challenges for tomato seedlings,potentially leading to uneven emergence,abnormal growth,and higher seedling mortality.This study evaluated the effectiveness of combining drum-priming with melatonin to alleviate chilling stress during these two critical stages.Tomato seeds were primed with melatonin at various concentrations and compared to untreated controls under chilling stress conditions.Higher concentrations of melatonin significantly accelerated emergence and improved early growth under stress.These stress-mitigating effects were evidenced by reductions in oxidative stress markers,such as malondialdehyde and hydrogen peroxide,along with increased total polyphenol and flavonoid contents.Furthermore,melatonin priming preserved photosynthetic efficiency,typically reduced by chilling stress,and enhanced the activities of antioxidant enzymes,including catalase and peroxidase.These biochemical changes reduced oxidative damage and promoted stress resilience.Melatonin also accelerated the expression of genes within the C-repeat binding factor pathway,which is crucial for cold acclimation.This suggests that melatonin priming enabled quicker adaptation to chilling stress following sowing and bolstered seedling resilience during subsequent growth stages.Overall,our results demonstrate that melatonin priming not only enhances germination but also significantly supports seedling growth under adverse conditions.The findings highlight melatonin as a promising tool in crop management strategies to improve resilience against sudden chilling stress.展开更多
Germinal matrix hemorrhage in preterm neonates often leads to white matter injury,contributing to long-term neurodevelopmental impairments.As resident brain immune cells,microglia play a complex role in injury respons...Germinal matrix hemorrhage in preterm neonates often leads to white matter injury,contributing to long-term neurodevelopmental impairments.As resident brain immune cells,microglia play a complex role in injury response,including inflammation and repair.Although colony-stimulating factor 1 receptor inhibitors such as PLX5622 enable the selective depletion of microglia,their therapeutic potential in neonatal germinal matrix hemorrhage remains underexplored.Here,we used a collagenase-induced germinal matrix hemorrhage model in postnatal day 5 mice,and intraperitoneally administered PLX562272 hours post-germinal matrix hemorrhage to achieve targeted,temporary microglial depletion during the peak injury response.We then assessed the effects of this delayed intervention on oligodendrocyte lineage cell maturation,white matter integrity,and neurobehavioral outcomes.Additionally,RNA sequencing data from a germinal matrix hemorrhage rat model were analyzed using weighted gene co-expression network analysis to identify the critical phases for interventions.RNA sequencing data revealed a critical period in which key synaptic functions declined while immune responses intensified post-germinal matrix hemorrhage,thus pinpointing the critical response phases for potential interventions.Delayed PLX5622 treatment effectively depleted activated microglia,protecting against white matter injury and enhancing oligodendrocyte lineage cell maturation and myelination in subcortical white matter regions.Moreover,magnetic resonance imaging analysis revealed reduced brain lesion volumes in treated mice.Behaviorally,PLX5622-treated mice exhibited significant improvements in motor coordination and reduced hyperactivity compared with vehicle-treated germinal matrix hemorrhage model mice.These findings suggest that,when timed to avoid interference with initial oligodendrocyte lineage cell proliferation,targeted microglial depletion with PLX5622 significantly mitigates white matter damage and improves neurobehavioral outcomes in neonatal germinal matrix hemorrhage.The present study highlights the therapeutic potential of selectively modulating microglial reactivity to support neurodevelopment in preterm infants with brain injury.展开更多
In grape breeding programs,the extensive planting of seedlings is a crucial aspect.However,grape seeds display distinct dormancy traits,necessitating a prolonged cold stratification process for dormancy release.In ord...In grape breeding programs,the extensive planting of seedlings is a crucial aspect.However,grape seeds display distinct dormancy traits,necessitating a prolonged cold stratification process for dormancy release.In order to enhance the efficiency of breeding programs,this study presents an innovative in vitro embryo germination technique that eliminates the requirement for cold stratification of seeds.The method involves the disruption of peripheral tissue in grape seed embryos using a straightforward mechanical technique,resulting in the efficient production of a substantial quantity of seed embryos,with a germination rate of up to 88% for these isolated embryos.These embryos are subsequently cultured in vitro to facilitate germination into seedlings,thereby eliminating the need for cold stratification.Consequently,grape seedlings can be obtained within a significantly reduced timeframe of 30-38 d,expediting the overall grape breeding process.This novel approach not only accelerates grape hybridization but also streamlines the selection of new grape varieties,contributing to an efficient and time-sensitive breeding methodology.展开更多
Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their po...Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their population dynamics may directly influence the future alpine ecosystems.However,little is known about how climate warming affect cushion population recruitment,especially at early life-history stages.In this study,we conducted a laboratory simulation of climate warming to detect the effects of warming temperature and associated moisture and light changes on seed germination and seedling growth of the typical alpine cushion plant Arenaria oreophila.Results suggested that increasing temperature indeed exerted strong constraints on the population recruitment processes.Specifically,increased temperatures could quickly initialize seed germination(4e6 days at higher temperatures vs.29 e32 days at low temperature,respectively,after sowing),accelerate them to reach the maximum germination percentage(9e19 days at higher temperatures vs.57e86 days at low temperature,respectively,after sowing)and significantly accelerate seedling growth rate.However,higher temperatures accelerated seedling mortality(more than 80%).In addition,lower light availability also increased seedling mortality though it could generally increase the final seed germination percentage.The effects of water might be dependent on temperature and light.All results suggested that cushion A.oreophila is quite sensitive to climate warming which strongly constrains its seedling establishment process.We,therefore,speculate that the continuing climate warming in future will exert uncertain risks in the persistence of cushion A.oreophila,possibly by constraining the process of seedling recruitments.展开更多
Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt toleran...Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.展开更多
The oilseed crop Camelina sativa exhibits salinity tolerance,but the effects on early growth stages across a range of different salts and in combination with salicylic acid(SA)have not been thoroughly evaluated.In thi...The oilseed crop Camelina sativa exhibits salinity tolerance,but the effects on early growth stages across a range of different salts and in combination with salicylic acid(SA)have not been thoroughly evaluated.In this study,seeds were germinated in varying concentrations of six salts(NaCl,CaCl_(2),ZnCl_(2),KCl,MgSO_(4),and Na2SO_(4))with or without 0.5 mM SA.Using the halotime model,we estimated salt thresholds for germination and parameters of seedling growth.Germination and seedling growth parameters of camelina significantly decreased with increasing salt concentration across all salt types.Salts containing Zn and SO_(4) were most detrimental to germination and seedling growth.Except for KCl,0.5 mM SA generally reduced the salinity tolerance threshold(Saltb(50))of camelina.Specifically,Saltb(50)was 21.5%higher for KCl and 16.1%,25.0%,54.9%,21.0%,and 5.6%lower for CaCl_(2),NaCl,MgSO_(4),Na2SO_(4),and ZnCl_(2),respectively,when 0.5 mM SA was compared to 0 mM SA.Furthermore,camelina seedling growth was consistently more sensitive than germination across all salt types.SA did not significantly enhance germination or seedling growth and was harmful when combined with certain salts or at the germination stage.It can be concluded that both the type of salt and the concentration of SA are as critical as the salt concentration in saline irrigation water.展开更多
[Objectives]To identify the drought resistance of main wheat varieties in Shandong Province and screen suitable cultivars for dryland cultivation.[Methods]Employing eight varieties including Jimai 60 as test materials...[Objectives]To identify the drought resistance of main wheat varieties in Shandong Province and screen suitable cultivars for dryland cultivation.[Methods]Employing eight varieties including Jimai 60 as test materials,this study simulated drought stress using 20%PEG-6000 and measured changes in germination-stage indicators.A comprehensive evaluation was conducted using the membership function method,incorporating relative coleoptile length,relative germ length,relative radicle length,relative germination rate,relative germination potential,and stress germination index.[Results]Drought stress not only reduced wheat seed germination rate but also inhibited the growth of the germ,coleoptile,and radicle.The D values of the eight varieties were ranked as follows:Jimai 60>Linmai 9>Yannong 999>Shannong 30>Shannong 28>Luyuan 502>Yannong 1212>Jimai 22.Based on D values,the eight dominant wheat varieties were classified into three categories:highly drought-resistant varieties(Linmai 9 and Jimai 60),moderately drought-resistant varieties(Yannong 999 and Shannong 30),and sensitive varieties(the others).Linmai 9 and Jimai 60 are recommended as suitable wheat varieties for dryland cultivation in Shandong Province.[Conclusions]Drought stress induced by 20%PEG-6000 reduced germination rate,germination potential,and germination index of wheat varieties while inhibiting the growth of coleoptiles and radicles.These indicators can provide a preliminary assessment of drought resistance in wheat cultivars.However,since filter paper was selected as the growth medium,root length measurement errors were introduced during root washing,leading to variations in final experimental results.Futuer studies could attempt using sterilized sand as an alternative growth medium.展开更多
The present study examines the toxicological effects and bioaccumulation of strontium(Sr^(2+))in two Moroccan wheat species,Triticum durum(Nachit)and Triticum aestivum(Ibtissam).Germination rates and seedling growth p...The present study examines the toxicological effects and bioaccumulation of strontium(Sr^(2+))in two Moroccan wheat species,Triticum durum(Nachit)and Triticum aestivum(Ibtissam).Germination rates and seedling growth parameters were evaluated under Sr^(2+)concentrations of 0,10,100,and 1000 mM.Results revealed a significant decline in germination rates as Sr^(2+)concentrations increased,with T.aestivum showing higher sensitivity compared to T.durum.Low Sr^(2+)concentrations(10 mM)initially promoted stem growth in T.aestivum,but growth declined sharply at higher concentrations.Both species sustained growth at moderate Sr^(2+)concentrations(100 mM),but experienced a substantial reduction at 1000 mM,with T.durum demonstrating slightly better tolerance.Elevated Sr^(2+)concentrations notably affected root length and stem branching,indicating severe phytotoxic effects.Enzyme activity assays showed that at low Sr^(2+)concentration(10 mM),GST,CAT,POD,and SOD activities were similar to the control.At moderate concentrations(100 mM),GST and POD activities increased,suggesting enhanced detoxification.At high concentrations(1000 mM),all enzyme activities were significantly elevated,reflecting a robust antioxidative defense mechanism.This study provides valuable insights into the toxicological impacts and physiological responses of these wheat species to Sr^(2+)stress.展开更多
Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roa...Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roasting time(0–50 min)at a specific temperature of 160?C on the physicochemical properties,bioactive compounds,antioxidant capacity,and volatile compounds of GFO from three flaxseed varieties was examined.An extension in roasting time increased the a^(*)value of GFO,while the L^(*)and b^(*)values of GFO decreased.Higher chlorophylls(1.63±0.01–2.18±0.01 mg/kg),carotenoids(2.25±0.00–2.69±0.02 mg/kg),total phenolic content(156.75±2.93–200.03±6.29 mg GAE/kg),phytosterol(1801.93±30.64–1965.23±13.71 mg GAE/kg)and DPPH(320.11±18.22–432.19±14.95μmol TE/kg)and ABTS radical scavenging activity(810.68±16.29–1119.01±14.25μmol TE/kg)were detected in GFO after roasting for 20–30 min.However,the total tocopherol and unsaturated fatty acid content declined,and the composition of fatty acids changed insignificantly(P>0.05).GC–MS recognized 68 volatile compounds in GFO,in which pyrazine imparted a nutty flavor to the GFO.This study provides important information for the development of high-quality GFO.展开更多
Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are kno...Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.展开更多
Salt stress is a major threat to crop agricultural productivity.Salinity affects plants’physiological and biochemical functions by hampering metabolic functions and decreasing photosynthetic rates.Salinity causes hyp...Salt stress is a major threat to crop agricultural productivity.Salinity affects plants’physiological and biochemical functions by hampering metabolic functions and decreasing photosynthetic rates.Salinity causes hyperosmotic and hyperionic stress,directly impairing plant growth.In this study,eggplant seeds primed with moringa leaf extract(5%,10%,and 15%),nano-titaniumdioxide(0.02%,0.04%,and 0.06%),and ascorbic acid(0.5,1,and 2 mM)at different NaCl salt(0,75,and 150 mM)concentration were grown.The germination attributes(final germination percentage,germination index,mean germination time,and mean germination rate)and growth(root length,shoot length,fresh biomass,and dry biomass)were enhanced in the primed seedlings by the different priming agents,more prominently in ascorbic acid primed seedlings.The accumulation of hydrogen peroxide was greater in seedlings with higher salt levels.Similarly,the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase)was higher in primed seedlings compared to the control.At 150 mM,the antioxidant capacity was higher than 75 mM,and the seedlings’sodiumand chloride content was higher.The results demonstrate that seedling germination,growth,and activity of the antioxidant enzymes in ascorbic acid-primed seedlings increase their tolerance to salinity.Therefore,using different ascorbic acid concentrations(0.5,1,and 2 mM)as a priming agent to enhance germination and growth in saline conditions has proven effective.展开更多
Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determin...Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and yield.This study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical insecticides.Seeds were primed for six hours,air-dried,and sown in the field.Germination rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop maturity.Cry1Ac gene expression was quantified to explore the influence of priming treatments on transgene activity.Results demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 treatment.These findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene expression.This study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.展开更多
[Objectives]Amygdalus tangutica is a new and excellent tree species for afforestation in arid areas of central China for soil and water conservation.It has the characteristics of drought resistance,cold resistance and...[Objectives]Amygdalus tangutica is a new and excellent tree species for afforestation in arid areas of central China for soil and water conservation.It has the characteristics of drought resistance,cold resistance and tolerance to poor soil,and has strong ecological,landscape and economic value.To provide a theoretical basis for the propagation and application of A.tangutica in Minqin and similar arid sandy areas of Northwest China,this paper explored its introduction and cultivation technology through field experiments,considering the natural geographical conditions of the study area.[Methods]High-quality seeds were introduced from forest farms of Chankou Town,Anding District,Dingxi City,and Dian ga Town,Diebu County,Gannan Prefecture,and the introduction and cultivation experiments of A.tangutica were carried out in the greenhouse of Wuwei Oasis Station.[Results](1)Soaking treatment at different temperatures combined with gibberellin treatment and stratification germination could effectively break the dormancy of A.tangutica seeds,thereby accelerating germination and shortening the germination cycle.The germination peak appeared 15-20 d after sowing,and the final germination rate could reach about 50%.(2)In the introduction and cultivation of A.tangutica in arid areas,large fruit seeds with higher thousand-grain weight should be given priority.The emergence rate of large fruit seeds was significantly higher than that of small fruit seeds,with the highest reaching 57%,while the highest of small fruit seeds was only 20%.Soaking treatment at different temperatures had no significant difference in the germination performance and germination potential of A.tangutica seeds,but the germination index of large fruit seeds was higher than that of small fruit seeds.(3)Under the current conditions,seedling propagation is a reliable way to propagate A.tangutica in arid areas.The cutting propagation technology,particularly hardwood cutting which exhibits an extremely low survival rate,is not yet mature and is currently unsuitable for large-scale production.Consequently,future efforts should prioritize more in-depth research on softwood cutting techniques.[Conclusions]This study provides a theoretical foundation for the propagation and broader application of A.tangutica in Minqin and similar arid sandy areas of Northwest China.展开更多
The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature...The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature.Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens.However,only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites.In this study,we conducted a metabolomic analysis on both blast-resistant(Digu)and-susceptible(Lijiangxintuanheigu)rice varieties to uncover novel metabolites that enhance blast resistance.We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling,based on the infected status of M.oryzae in rice and the observed differences in shikimate accumulation between the two varieties.Following metabolomic analysis,we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control.Among these,apigenin-7-glucoside,rhamnetin,and spireoside were found to be effective in controlling blast disease,with spireoside demonstrating the most pronounced efficacy.We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M.oryzae,primarily through disrupting cell membrane integrity.However,spireoside did not induce rice immunity.Furthermore,spireoside was also effective in controlling sheath blight disease.Thus,spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases.展开更多
Low-temperature(LT)stress is a significant abiotic stress in rice growth,especially under direct seeding cultivation,where low temperatures can significantly affect seed germination and seedling growth of direct-seede...Low-temperature(LT)stress is a significant abiotic stress in rice growth,especially under direct seeding cultivation,where low temperatures can significantly affect seed germination and seedling growth of direct-seeded rice,thereby impacting the final yield of rice.In this study,we have identified a trehalose synthesis pathway gene,trehalose-6-phosphate phosphatase 3(Os TPP3),involved in the regulation of low-temperature(LT)germination in rice,as well as its upstream regulatory factor,the ABA signaling pathway gene OsbZIP23.LT stress induced the accumulation of ABA by upregulating the expression of Os NCED3.Consistently,the overexpression of Os NCED3 significantly inhibited seed germination under LT.RT-q PCR experiments found that the expression of OsbZIP23 was also significantly induced under LT stress and ABA treatment.Overexpression of OsbZIP23 has increased the sensitivity to LT stress of rice seed,resembling the phenotype of Os NCED3 overexpressing seeds.Furthermore,both LT stress and exogenous ABA treatment increased the trehalose content in WT seeds by upregulating the expression of Os TPP3.Enhancing the expression of Os TPP3 or application of exogenous trehalose have significantly increased the sensitivity to LT stress during seed germination.Transcriptional activation and yeast one-hybrid assays demonstrated that OsbZIP23 bound to the promoter of Os TPP3 and activated its expression,which was intensified by LT stress or the application of ABA.Our study discovered an ABAdependent OsbZIP23–Os TPP3 module that responds to LT stress,inhibiting seed germination under LT conditions by increasing trehalose accumulation,thus might balance the growth and stress resistance and provide a new insight into the genetic improvement of rice cultivars with better LT germination performance.展开更多
This study explores the dose-dependent impacts of light crude oil contamination on seed germination and seedling growth in Zea mays L.(maize),a critical agricultural species.We hypothesized that higher concentrations ...This study explores the dose-dependent impacts of light crude oil contamination on seed germination and seedling growth in Zea mays L.(maize),a critical agricultural species.We hypothesized that higher concentrations of light crude oil in soil would progressively suppress germination kinetics and seedling vigor.To test this,Zea mays seeds were exposed to light crude oil at concentrations ranging from 0.0%to 10.0%(v/v)mixed with soil.The experimental design included a control group treated with distilled water to establish baseline germination and growth metrics.Results revealed a clear concentration-dependent phytotoxic effect.Germination percentage significantly declined from 93.3%in the control to 40.0%at 8.0%(v/v)oil concentration(p<0.05),with complete inhibition of germination observed at 10.0%(v/v).Seedling vigor,assessed through shoot length,exhibited a drastic 93%reduction at 8.0%(v/v)compared to the control,while concentrations up to 4.0%(v/v)showed minimal impact on growth.Germination indices,such as Mean Germination Time(MGT)and Coefficient of Velocity of Germination(CGV),further corroborated the inhibitory effects,with higher oil concentrations delaying and reducing germination rates.These findings suggest a phytotoxicity threshold for Zea mays around 6.0%(v/v)light crude oil,beyond which severe impairments occur.The data provide valuable insights for developing phytoremediation strategies in oil-contaminated agricultural soils.However,the study’s limitations include its focus on a single species and the absence of field-based validation,necessitating further research to confirm these findings under natural conditions and across diverse plant species.展开更多
Porcine epidemic diarrhea virus(PEDV),an enteric coronavirus,is widely spread worldwide and causes huge economic losses.The effective measure to control the viral infection is to develop ideal vaccines.Here,the collag...Porcine epidemic diarrhea virus(PEDV),an enteric coronavirus,is widely spread worldwide and causes huge economic losses.The effective measure to control the viral infection is to develop ideal vaccines.Here,the collagenase equivalent domain(COE)of PEDV was displayed on the surface of nanoparticles(NPs)in order to develop a newer,safer and more effective subunit vaccine against PEDV.The monomeric COE was displayed on the mi3 protein,which self-assembles into nanoparticles composed of 60 subunits,using the SpyTag/SpyCatcher system.The size,zeta potential,microstructure of the COE-mi3 virus-like particles(VLPs)were investigated.The COE-mi3 VLPs that possessed good security,stability and better retention can be more efficiently taken up by antigen-presenting cells(APCs)and help promote dendritic cells(DCs)maturation.Moreover,COE-mi3 VLPs could prominently improve specifc antibody levels including neutralizing antibodies(NAbs),and serum IgG,mucosal IgA.Moreover,COE-mi3 VLPs elicited more activation of CD4^(+)and CD8^(+)T cells and production of IFN-γand IL-4 cytokines.In particular,COE-mi3 VLPs is an effectual antigen-delivery platform to enhance germinal center(GC)B cell responses.This structure-based self-assembly of NP gives great potential to be developed as a new subunit vaccines attractive platform,and may also provide new ideas for the development of other enteric coronavirus vaccines.展开更多
文摘The objective of this research is to lift the dormancy of seeds of S. kunthianum in with a view to domesticating them. In this experiment, we used the soaking of seeds in sulfuric acid for different durations (10 min, 30 min and 1 h). The treated seeds were sown in transparent germinators. The explants obtained were then transplanted into pots containing five types of substrate (black earth, fine sand, sawdust, mixtures 1/1 of sawdust/black earth and 1/1 of sawdust/fine sand). This second test made it possible to determine the rate of germination on the substrate. The results obtained compared to those of the control showed a clear reduction in the latency time or germination time (62 hours) instead of 6 days (control), in the germination time 3 days/7 days (control) and a very high germination rate (100%). At the 5% threshold, soaking for 10 min and 30 min (100%) is the best result on the germination of S. kunthianum seeds. Soaking for 1 hour gives (7%) very low. After transplantation of the explants, the percentages obtained independently of the treatments show that the 1/1 mixtures of sawdust/black earth (75.19%), black earth (73.33%) and sawdust/fine sand (66.30%) have a higher performance than the others. The type of germination is epigeal. This work makes it possible to domesticate S. kunthianum in Chad.
文摘Aconitum heterophyllum Wall.ex Royle,an endangered Ayurvedic medicinal plant,is predominantly found in the alpine and subalpine zones of the Himalayas.This study aimed to enhance seed germination under various soil conditions,to facilitate conservation and provide opportunities for income generation through large-scale cultivation.The selection of soil treatments was based on their contrasting physicochemical properties and known influence on seed germination in related high altitude species.The study was conducted in a mist chamber at the Deoban nursery in Dehradun,India,using five soil conditions:normal forest soil(NS),vermiculite(Vc),vermiculite+normal forest soil(Vc+NS),normal forest soil+sand(NS+S),and a mixture of soil,sand,vermiculite,and vermicompost(NS+S+Vc+VCo).All the soil treatments were replicated ten times under the controlled environmental conditions.These substrates were selected to represent a gradient of water retention,aeration,nutrient content,and organic matter composition,which are known to affect seed germination and early seedling development.Results showed significant different among treatments in terms of physical and chemical soil properties in sand,silt,clay,pH,organic carbon,organic matter,total nitrogen,total phosphorus,as well as growth parameters such as,seed vigor index,root length,plant length,and leaf number across soil conditions.Among all treatments,vermiculite alone showed the highest germination rate(73.33%),peak germination value(0.719),and seed vigor index(24.02),along with superior growth parameters and the lowest seed mortality index(2.45).These findings in addition to its low seed mortality underscore vermiculite's potential as a suitable growth medium for the ex-situ cultivation of A.heterophyllum offering a promising alternative to wild harvesting while supporting conservation and sustainable use of this valuable Himalayan species.
文摘Seed vigor is critical for uniform germination and emergence,directly influencing subsequent seedling development.This is especially important under both normal and stress conditions that may arise post-sowing.Chilling stress during emergence and early growth poses significant challenges for tomato seedlings,potentially leading to uneven emergence,abnormal growth,and higher seedling mortality.This study evaluated the effectiveness of combining drum-priming with melatonin to alleviate chilling stress during these two critical stages.Tomato seeds were primed with melatonin at various concentrations and compared to untreated controls under chilling stress conditions.Higher concentrations of melatonin significantly accelerated emergence and improved early growth under stress.These stress-mitigating effects were evidenced by reductions in oxidative stress markers,such as malondialdehyde and hydrogen peroxide,along with increased total polyphenol and flavonoid contents.Furthermore,melatonin priming preserved photosynthetic efficiency,typically reduced by chilling stress,and enhanced the activities of antioxidant enzymes,including catalase and peroxidase.These biochemical changes reduced oxidative damage and promoted stress resilience.Melatonin also accelerated the expression of genes within the C-repeat binding factor pathway,which is crucial for cold acclimation.This suggests that melatonin priming enabled quicker adaptation to chilling stress following sowing and bolstered seedling resilience during subsequent growth stages.Overall,our results demonstrate that melatonin priming not only enhances germination but also significantly supports seedling growth under adverse conditions.The findings highlight melatonin as a promising tool in crop management strategies to improve resilience against sudden chilling stress.
基金supported by the National Key Research and Development Program of China,No.2022YFC2704801(to CZhu)the National Natural Science Foundation of China,Nos.U21A20347(to CZhu),82203969(to YX),82371472(to XZ)+3 种基金Health Commission of Henan Province,Nos.SBGJ202303039(to XZ),SBGJ202301009(to CZhu),YQRC2024018(to XZ),YQRC2024019(to YX)Henan Science and Technology Department,Nos.242102311054(to XZ),241111521300(to CZhu),GZS2023003(to XW)Swedish Research Council,Nos.2022-01019(to CZhu),2021-01950(to XW)Swedish Governmental Grants to Scientists Working in Healthcare,Nos.ALFGBG-1005209(to CZhu),ALFBG-1005257(to XW),ALFGBG-965197(to CZhu).
文摘Germinal matrix hemorrhage in preterm neonates often leads to white matter injury,contributing to long-term neurodevelopmental impairments.As resident brain immune cells,microglia play a complex role in injury response,including inflammation and repair.Although colony-stimulating factor 1 receptor inhibitors such as PLX5622 enable the selective depletion of microglia,their therapeutic potential in neonatal germinal matrix hemorrhage remains underexplored.Here,we used a collagenase-induced germinal matrix hemorrhage model in postnatal day 5 mice,and intraperitoneally administered PLX562272 hours post-germinal matrix hemorrhage to achieve targeted,temporary microglial depletion during the peak injury response.We then assessed the effects of this delayed intervention on oligodendrocyte lineage cell maturation,white matter integrity,and neurobehavioral outcomes.Additionally,RNA sequencing data from a germinal matrix hemorrhage rat model were analyzed using weighted gene co-expression network analysis to identify the critical phases for interventions.RNA sequencing data revealed a critical period in which key synaptic functions declined while immune responses intensified post-germinal matrix hemorrhage,thus pinpointing the critical response phases for potential interventions.Delayed PLX5622 treatment effectively depleted activated microglia,protecting against white matter injury and enhancing oligodendrocyte lineage cell maturation and myelination in subcortical white matter regions.Moreover,magnetic resonance imaging analysis revealed reduced brain lesion volumes in treated mice.Behaviorally,PLX5622-treated mice exhibited significant improvements in motor coordination and reduced hyperactivity compared with vehicle-treated germinal matrix hemorrhage model mice.These findings suggest that,when timed to avoid interference with initial oligodendrocyte lineage cell proliferation,targeted microglial depletion with PLX5622 significantly mitigates white matter damage and improves neurobehavioral outcomes in neonatal germinal matrix hemorrhage.The present study highlights the therapeutic potential of selectively modulating microglial reactivity to support neurodevelopment in preterm infants with brain injury.
基金supported by Natural Science Foundation of Beijing Academy of Agriculture and Forestry Sciences(Grant No.QNJJ202301)the Youth Research Foundation of Institute of Forestry and Pomology+2 种基金Beijing Academy of Agriculture and Forestry Science(Grant No.LGJJ202202)the BAAFS Funding for the Development of Distinguished Scientist(Grant No.JKZX202402)the Beijing Natural Science Foundation(Grant No.6242019)。
文摘In grape breeding programs,the extensive planting of seedlings is a crucial aspect.However,grape seeds display distinct dormancy traits,necessitating a prolonged cold stratification process for dormancy release.In order to enhance the efficiency of breeding programs,this study presents an innovative in vitro embryo germination technique that eliminates the requirement for cold stratification of seeds.The method involves the disruption of peripheral tissue in grape seed embryos using a straightforward mechanical technique,resulting in the efficient production of a substantial quantity of seed embryos,with a germination rate of up to 88% for these isolated embryos.These embryos are subsequently cultured in vitro to facilitate germination into seedlings,thereby eliminating the need for cold stratification.Consequently,grape seedlings can be obtained within a significantly reduced timeframe of 30-38 d,expediting the overall grape breeding process.This novel approach not only accelerates grape hybridization but also streamlines the selection of new grape varieties,contributing to an efficient and time-sensitive breeding methodology.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502 to H.S.)the Yunnan Applied Basic Research Project(202001AT070060 to J.G.C.)+1 种基金the CAS“Light ofWest China”Program(J.G.C.)the Young Academic and Technical Leader Raising Foundation of Yunnan Province(202205AC160053 to J.G.C.).
文摘Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their population dynamics may directly influence the future alpine ecosystems.However,little is known about how climate warming affect cushion population recruitment,especially at early life-history stages.In this study,we conducted a laboratory simulation of climate warming to detect the effects of warming temperature and associated moisture and light changes on seed germination and seedling growth of the typical alpine cushion plant Arenaria oreophila.Results suggested that increasing temperature indeed exerted strong constraints on the population recruitment processes.Specifically,increased temperatures could quickly initialize seed germination(4e6 days at higher temperatures vs.29 e32 days at low temperature,respectively,after sowing),accelerate them to reach the maximum germination percentage(9e19 days at higher temperatures vs.57e86 days at low temperature,respectively,after sowing)and significantly accelerate seedling growth rate.However,higher temperatures accelerated seedling mortality(more than 80%).In addition,lower light availability also increased seedling mortality though it could generally increase the final seed germination percentage.The effects of water might be dependent on temperature and light.All results suggested that cushion A.oreophila is quite sensitive to climate warming which strongly constrains its seedling establishment process.We,therefore,speculate that the continuing climate warming in future will exert uncertain risks in the persistence of cushion A.oreophila,possibly by constraining the process of seedling recruitments.
基金funded by the Natural Science Foundation of Shandong Province(ZR2020MC144)the Scientific Research Training Program for Undergraduates of Qufu Normal University(XJ2024016).
文摘Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.
基金the Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT) Sari Agricultural Sciences and Natural Resources University (SANRU) for the use of the services and financial supports of this research
文摘The oilseed crop Camelina sativa exhibits salinity tolerance,but the effects on early growth stages across a range of different salts and in combination with salicylic acid(SA)have not been thoroughly evaluated.In this study,seeds were germinated in varying concentrations of six salts(NaCl,CaCl_(2),ZnCl_(2),KCl,MgSO_(4),and Na2SO_(4))with or without 0.5 mM SA.Using the halotime model,we estimated salt thresholds for germination and parameters of seedling growth.Germination and seedling growth parameters of camelina significantly decreased with increasing salt concentration across all salt types.Salts containing Zn and SO_(4) were most detrimental to germination and seedling growth.Except for KCl,0.5 mM SA generally reduced the salinity tolerance threshold(Saltb(50))of camelina.Specifically,Saltb(50)was 21.5%higher for KCl and 16.1%,25.0%,54.9%,21.0%,and 5.6%lower for CaCl_(2),NaCl,MgSO_(4),Na2SO_(4),and ZnCl_(2),respectively,when 0.5 mM SA was compared to 0 mM SA.Furthermore,camelina seedling growth was consistently more sensitive than germination across all salt types.SA did not significantly enhance germination or seedling growth and was harmful when combined with certain salts or at the germination stage.It can be concluded that both the type of salt and the concentration of SA are as critical as the salt concentration in saline irrigation water.
基金Supported by National Wheat Industry Technology System"Linyi Integrated Experimental Station"(CARS-03-66)Shandong Provincial Modern Agricultural Industry Technology System"Linyi Integrated Experimental Station"(SDATT-01-18).
文摘[Objectives]To identify the drought resistance of main wheat varieties in Shandong Province and screen suitable cultivars for dryland cultivation.[Methods]Employing eight varieties including Jimai 60 as test materials,this study simulated drought stress using 20%PEG-6000 and measured changes in germination-stage indicators.A comprehensive evaluation was conducted using the membership function method,incorporating relative coleoptile length,relative germ length,relative radicle length,relative germination rate,relative germination potential,and stress germination index.[Results]Drought stress not only reduced wheat seed germination rate but also inhibited the growth of the germ,coleoptile,and radicle.The D values of the eight varieties were ranked as follows:Jimai 60>Linmai 9>Yannong 999>Shannong 30>Shannong 28>Luyuan 502>Yannong 1212>Jimai 22.Based on D values,the eight dominant wheat varieties were classified into three categories:highly drought-resistant varieties(Linmai 9 and Jimai 60),moderately drought-resistant varieties(Yannong 999 and Shannong 30),and sensitive varieties(the others).Linmai 9 and Jimai 60 are recommended as suitable wheat varieties for dryland cultivation in Shandong Province.[Conclusions]Drought stress induced by 20%PEG-6000 reduced germination rate,germination potential,and germination index of wheat varieties while inhibiting the growth of coleoptiles and radicles.These indicators can provide a preliminary assessment of drought resistance in wheat cultivars.However,since filter paper was selected as the growth medium,root length measurement errors were introduced during root washing,leading to variations in final experimental results.Futuer studies could attempt using sterilized sand as an alternative growth medium.
文摘The present study examines the toxicological effects and bioaccumulation of strontium(Sr^(2+))in two Moroccan wheat species,Triticum durum(Nachit)and Triticum aestivum(Ibtissam).Germination rates and seedling growth parameters were evaluated under Sr^(2+)concentrations of 0,10,100,and 1000 mM.Results revealed a significant decline in germination rates as Sr^(2+)concentrations increased,with T.aestivum showing higher sensitivity compared to T.durum.Low Sr^(2+)concentrations(10 mM)initially promoted stem growth in T.aestivum,but growth declined sharply at higher concentrations.Both species sustained growth at moderate Sr^(2+)concentrations(100 mM),but experienced a substantial reduction at 1000 mM,with T.durum demonstrating slightly better tolerance.Elevated Sr^(2+)concentrations notably affected root length and stem branching,indicating severe phytotoxic effects.Enzyme activity assays showed that at low Sr^(2+)concentration(10 mM),GST,CAT,POD,and SOD activities were similar to the control.At moderate concentrations(100 mM),GST and POD activities increased,suggesting enhanced detoxification.At high concentrations(1000 mM),all enzyme activities were significantly elevated,reflecting a robust antioxidative defense mechanism.This study provides valuable insights into the toxicological impacts and physiological responses of these wheat species to Sr^(2+)stress.
基金supported by Shaanxi Province Qin Chuangyuan“Scientist+Engineer”team construction project(2024QCY-KXJ-079)Shaanxi Science and Technology Innovation Team Project(2024RSCXTD-70)for the financial support。
文摘Roasting is important for improving the content of bioactive compounds and enhancing the flavor of flaxseed oil.However,the effect of roasting time on germinated flaxseed oil(GFO)is unclear.Herein,the influence of roasting time(0–50 min)at a specific temperature of 160?C on the physicochemical properties,bioactive compounds,antioxidant capacity,and volatile compounds of GFO from three flaxseed varieties was examined.An extension in roasting time increased the a^(*)value of GFO,while the L^(*)and b^(*)values of GFO decreased.Higher chlorophylls(1.63±0.01–2.18±0.01 mg/kg),carotenoids(2.25±0.00–2.69±0.02 mg/kg),total phenolic content(156.75±2.93–200.03±6.29 mg GAE/kg),phytosterol(1801.93±30.64–1965.23±13.71 mg GAE/kg)and DPPH(320.11±18.22–432.19±14.95μmol TE/kg)and ABTS radical scavenging activity(810.68±16.29–1119.01±14.25μmol TE/kg)were detected in GFO after roasting for 20–30 min.However,the total tocopherol and unsaturated fatty acid content declined,and the composition of fatty acids changed insignificantly(P>0.05).GC–MS recognized 68 volatile compounds in GFO,in which pyrazine imparted a nutty flavor to the GFO.This study provides important information for the development of high-quality GFO.
基金supported by the National Natural Science Foundation of China(31901300).
文摘Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.
文摘Salt stress is a major threat to crop agricultural productivity.Salinity affects plants’physiological and biochemical functions by hampering metabolic functions and decreasing photosynthetic rates.Salinity causes hyperosmotic and hyperionic stress,directly impairing plant growth.In this study,eggplant seeds primed with moringa leaf extract(5%,10%,and 15%),nano-titaniumdioxide(0.02%,0.04%,and 0.06%),and ascorbic acid(0.5,1,and 2 mM)at different NaCl salt(0,75,and 150 mM)concentration were grown.The germination attributes(final germination percentage,germination index,mean germination time,and mean germination rate)and growth(root length,shoot length,fresh biomass,and dry biomass)were enhanced in the primed seedlings by the different priming agents,more prominently in ascorbic acid primed seedlings.The accumulation of hydrogen peroxide was greater in seedlings with higher salt levels.Similarly,the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase)was higher in primed seedlings compared to the control.At 150 mM,the antioxidant capacity was higher than 75 mM,and the seedlings’sodiumand chloride content was higher.The results demonstrate that seedling germination,growth,and activity of the antioxidant enzymes in ascorbic acid-primed seedlings increase their tolerance to salinity.Therefore,using different ascorbic acid concentrations(0.5,1,and 2 mM)as a priming agent to enhance germination and growth in saline conditions has proven effective.
基金National Natural Science Foundation of China(3216045632360474+2 种基金32360486)grants from the Provincial Basic Research Program(Natural Science)([2020]1Z018)Provincial Key Technology R&D Program([2021]YiBan272).
文摘Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and yield.This study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical insecticides.Seeds were primed for six hours,air-dried,and sown in the field.Germination rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop maturity.Cry1Ac gene expression was quantified to explore the influence of priming treatments on transgene activity.Results demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 treatment.These findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene expression.This study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.
基金Supported by Gansu Provincial Key Talent Project(GZTZ20240415)The Central Financial Forestry Science and Technology Promotion Demonstration Fund Project(2024ZYTG04)+1 种基金Gansu Provincial Key Research and Development Program(25YFFA073)"Jiebangguashuai"Project for Key Technology Research and Development of the"Three North"Program of National Forestry and Grassland Administration(202401-05).
文摘[Objectives]Amygdalus tangutica is a new and excellent tree species for afforestation in arid areas of central China for soil and water conservation.It has the characteristics of drought resistance,cold resistance and tolerance to poor soil,and has strong ecological,landscape and economic value.To provide a theoretical basis for the propagation and application of A.tangutica in Minqin and similar arid sandy areas of Northwest China,this paper explored its introduction and cultivation technology through field experiments,considering the natural geographical conditions of the study area.[Methods]High-quality seeds were introduced from forest farms of Chankou Town,Anding District,Dingxi City,and Dian ga Town,Diebu County,Gannan Prefecture,and the introduction and cultivation experiments of A.tangutica were carried out in the greenhouse of Wuwei Oasis Station.[Results](1)Soaking treatment at different temperatures combined with gibberellin treatment and stratification germination could effectively break the dormancy of A.tangutica seeds,thereby accelerating germination and shortening the germination cycle.The germination peak appeared 15-20 d after sowing,and the final germination rate could reach about 50%.(2)In the introduction and cultivation of A.tangutica in arid areas,large fruit seeds with higher thousand-grain weight should be given priority.The emergence rate of large fruit seeds was significantly higher than that of small fruit seeds,with the highest reaching 57%,while the highest of small fruit seeds was only 20%.Soaking treatment at different temperatures had no significant difference in the germination performance and germination potential of A.tangutica seeds,but the germination index of large fruit seeds was higher than that of small fruit seeds.(3)Under the current conditions,seedling propagation is a reliable way to propagate A.tangutica in arid areas.The cutting propagation technology,particularly hardwood cutting which exhibits an extremely low survival rate,is not yet mature and is currently unsuitable for large-scale production.Consequently,future efforts should prioritize more in-depth research on softwood cutting techniques.[Conclusions]This study provides a theoretical foundation for the propagation and broader application of A.tangutica in Minqin and similar arid sandy areas of Northwest China.
基金supported by the National Natural Science Foundation of China(Grant Nos.32172419,32372555,32121003,32425005,32072407,and 32272033)the National Key Research and Development Program of China(Grant No.2021YFA1300702)the Sichuan Science and Technology Program,China(Grant Nos.2023NSFSC0005,2023NSFSC1996,2024NSFSC0322,2024YFNH0014,2022NSFSC1755,2022-NSFSC1650,2022NSFSC0156,and 2022NSFSC0166).
文摘The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature.Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens.However,only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites.In this study,we conducted a metabolomic analysis on both blast-resistant(Digu)and-susceptible(Lijiangxintuanheigu)rice varieties to uncover novel metabolites that enhance blast resistance.We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling,based on the infected status of M.oryzae in rice and the observed differences in shikimate accumulation between the two varieties.Following metabolomic analysis,we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control.Among these,apigenin-7-glucoside,rhamnetin,and spireoside were found to be effective in controlling blast disease,with spireoside demonstrating the most pronounced efficacy.We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M.oryzae,primarily through disrupting cell membrane integrity.However,spireoside did not induce rice immunity.Furthermore,spireoside was also effective in controlling sheath blight disease.Thus,spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases.
基金funded by the Science and Technology Innovation Program of Hunan Province(2023NK1010,2024NK1010,2023NK2002)National Natural Science Foundation of China(U21A20208,32171927)+3 种基金Natural Science Foundation of Hunan Province(2025JJ30010,2023JJ40318)Guangdong Basic and Applied Basic Research Foundation(2022A1515111230)Shenzhen Science and Technology Program(JCYJ20220531103803008)the Hong Kong Research Grant Council(Ao E/M-05/12,Ao E/M-403/16,GRF12102423,12101722,12105824)。
文摘Low-temperature(LT)stress is a significant abiotic stress in rice growth,especially under direct seeding cultivation,where low temperatures can significantly affect seed germination and seedling growth of direct-seeded rice,thereby impacting the final yield of rice.In this study,we have identified a trehalose synthesis pathway gene,trehalose-6-phosphate phosphatase 3(Os TPP3),involved in the regulation of low-temperature(LT)germination in rice,as well as its upstream regulatory factor,the ABA signaling pathway gene OsbZIP23.LT stress induced the accumulation of ABA by upregulating the expression of Os NCED3.Consistently,the overexpression of Os NCED3 significantly inhibited seed germination under LT.RT-q PCR experiments found that the expression of OsbZIP23 was also significantly induced under LT stress and ABA treatment.Overexpression of OsbZIP23 has increased the sensitivity to LT stress of rice seed,resembling the phenotype of Os NCED3 overexpressing seeds.Furthermore,both LT stress and exogenous ABA treatment increased the trehalose content in WT seeds by upregulating the expression of Os TPP3.Enhancing the expression of Os TPP3 or application of exogenous trehalose have significantly increased the sensitivity to LT stress during seed germination.Transcriptional activation and yeast one-hybrid assays demonstrated that OsbZIP23 bound to the promoter of Os TPP3 and activated its expression,which was intensified by LT stress or the application of ABA.Our study discovered an ABAdependent OsbZIP23–Os TPP3 module that responds to LT stress,inhibiting seed germination under LT conditions by increasing trehalose accumulation,thus might balance the growth and stress resistance and provide a new insight into the genetic improvement of rice cultivars with better LT germination performance.
文摘This study explores the dose-dependent impacts of light crude oil contamination on seed germination and seedling growth in Zea mays L.(maize),a critical agricultural species.We hypothesized that higher concentrations of light crude oil in soil would progressively suppress germination kinetics and seedling vigor.To test this,Zea mays seeds were exposed to light crude oil at concentrations ranging from 0.0%to 10.0%(v/v)mixed with soil.The experimental design included a control group treated with distilled water to establish baseline germination and growth metrics.Results revealed a clear concentration-dependent phytotoxic effect.Germination percentage significantly declined from 93.3%in the control to 40.0%at 8.0%(v/v)oil concentration(p<0.05),with complete inhibition of germination observed at 10.0%(v/v).Seedling vigor,assessed through shoot length,exhibited a drastic 93%reduction at 8.0%(v/v)compared to the control,while concentrations up to 4.0%(v/v)showed minimal impact on growth.Germination indices,such as Mean Germination Time(MGT)and Coefficient of Velocity of Germination(CGV),further corroborated the inhibitory effects,with higher oil concentrations delaying and reducing germination rates.These findings suggest a phytotoxicity threshold for Zea mays around 6.0%(v/v)light crude oil,beyond which severe impairments occur.The data provide valuable insights for developing phytoremediation strategies in oil-contaminated agricultural soils.However,the study’s limitations include its focus on a single species and the absence of field-based validation,necessitating further research to confirm these findings under natural conditions and across diverse plant species.
基金supported by the Major Scientific and Technological Project of the Henan Province,China(221100110600)the Beijing Life Science Academy,China(2024500CA0010)+1 种基金the Major Program of National Natural Science Foundation of China(32192452)the Chinese Postdoctoral Science Foundation(2023M743209)。
文摘Porcine epidemic diarrhea virus(PEDV),an enteric coronavirus,is widely spread worldwide and causes huge economic losses.The effective measure to control the viral infection is to develop ideal vaccines.Here,the collagenase equivalent domain(COE)of PEDV was displayed on the surface of nanoparticles(NPs)in order to develop a newer,safer and more effective subunit vaccine against PEDV.The monomeric COE was displayed on the mi3 protein,which self-assembles into nanoparticles composed of 60 subunits,using the SpyTag/SpyCatcher system.The size,zeta potential,microstructure of the COE-mi3 virus-like particles(VLPs)were investigated.The COE-mi3 VLPs that possessed good security,stability and better retention can be more efficiently taken up by antigen-presenting cells(APCs)and help promote dendritic cells(DCs)maturation.Moreover,COE-mi3 VLPs could prominently improve specifc antibody levels including neutralizing antibodies(NAbs),and serum IgG,mucosal IgA.Moreover,COE-mi3 VLPs elicited more activation of CD4^(+)and CD8^(+)T cells and production of IFN-γand IL-4 cytokines.In particular,COE-mi3 VLPs is an effectual antigen-delivery platform to enhance germinal center(GC)B cell responses.This structure-based self-assembly of NP gives great potential to be developed as a new subunit vaccines attractive platform,and may also provide new ideas for the development of other enteric coronavirus vaccines.
