The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards...The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards. It can also attacks pears, walnuts and other tree fruits. The codling moth is an important invasive species in China, which first appeared in Kuerle, Xijiang in 1953 and rapidly expanded in the whole area, in 1987 the codling moth spread to Dunhuang, Gansu. Recently, researchers from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Nanjing Agricultural University and so on initiated a genome-sequencing project of the codling moth. The genome size of the codling moth is 650 Mb, as estimated by flow cytometry and survey sequencing. Since the heterozygousity is a potential obstacle in sequencing the insect genome, the research consortium carefully measured the heterozygousity of the codling moth by SNP analysis and 17-mer estimation, showing that the heterozygousity of the codling moth is around 0.3-0.6%. The whole genome shotgun strategy is adopted and a sequencing plan has been made and officially started. The availability of the codling moth genome should be of great value to uncover the molecular invasion mechanism, high adaptive ability to the stress and to develop efficient control strategies of this pest worldwide and in China.展开更多
With the increasing demand for diversified health,the characteristic probiotics species resources need to be expanded.Here,a novel marine multi-stress-tolerant aromatic yeast Pichia kudriavzevii HJ2 was identified fro...With the increasing demand for diversified health,the characteristic probiotics species resources need to be expanded.Here,a novel marine multi-stress-tolerant aromatic yeast Pichia kudriavzevii HJ2 was identified from marine mangrove.Based on the characterization of probiotic properties in vivo and in vitro,the yeast could assimilate multiple carbon sources,and generate volatile flavor metabolites,achieved a 79.98%cholesterol removal rate with efficient in vivo survival and adhesion capabilities,and showed a strong antioxidant capacity.HJ2 also released hydrolase and fermented dietary fiber to yield SCFAs,contributing to disease prevention and treatment.Then,the safety was assessed according to phenotype and whole genome-sequencing analysis.No virulence genes or ARGs were annotated in the HJ2 genome.A few biogenic amines(BA)-related genes were observed,but the production of various BA and total BA were far below the food-defined thresholds.HJ2 did not produce virulence enzymes such as DNase,phospholipase,gelatinase,or coagulase,and exhibited sensitivity to amphotericin B and anidulafungin withoutβ-hemolysis.An in vivo acute toxicity test conducted on mice demonstrated that HJ2 is safe.In conclusion,HJ2 could be considered safe and be further used as a potential probiotic candidate for further clinical trials.展开更多
文摘The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards. It can also attacks pears, walnuts and other tree fruits. The codling moth is an important invasive species in China, which first appeared in Kuerle, Xijiang in 1953 and rapidly expanded in the whole area, in 1987 the codling moth spread to Dunhuang, Gansu. Recently, researchers from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Nanjing Agricultural University and so on initiated a genome-sequencing project of the codling moth. The genome size of the codling moth is 650 Mb, as estimated by flow cytometry and survey sequencing. Since the heterozygousity is a potential obstacle in sequencing the insect genome, the research consortium carefully measured the heterozygousity of the codling moth by SNP analysis and 17-mer estimation, showing that the heterozygousity of the codling moth is around 0.3-0.6%. The whole genome shotgun strategy is adopted and a sequencing plan has been made and officially started. The availability of the codling moth genome should be of great value to uncover the molecular invasion mechanism, high adaptive ability to the stress and to develop efficient control strategies of this pest worldwide and in China.
基金supported by the Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development[Grant No.GUIKEZY21195021]the Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China[Grant No.2019GXNSFFA245011]+2 种基金the Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development[Grant No.20231012]the Funding Project of Technological Development from Angel Yeast[Chongzuo]Co.,Ltd.[Grant No.JS1006020230722019]the Basic Research Fund of Guangxi Academy of Sciences[Grant No.CQ-C-202202].
文摘With the increasing demand for diversified health,the characteristic probiotics species resources need to be expanded.Here,a novel marine multi-stress-tolerant aromatic yeast Pichia kudriavzevii HJ2 was identified from marine mangrove.Based on the characterization of probiotic properties in vivo and in vitro,the yeast could assimilate multiple carbon sources,and generate volatile flavor metabolites,achieved a 79.98%cholesterol removal rate with efficient in vivo survival and adhesion capabilities,and showed a strong antioxidant capacity.HJ2 also released hydrolase and fermented dietary fiber to yield SCFAs,contributing to disease prevention and treatment.Then,the safety was assessed according to phenotype and whole genome-sequencing analysis.No virulence genes or ARGs were annotated in the HJ2 genome.A few biogenic amines(BA)-related genes were observed,but the production of various BA and total BA were far below the food-defined thresholds.HJ2 did not produce virulence enzymes such as DNase,phospholipase,gelatinase,or coagulase,and exhibited sensitivity to amphotericin B and anidulafungin withoutβ-hemolysis.An in vivo acute toxicity test conducted on mice demonstrated that HJ2 is safe.In conclusion,HJ2 could be considered safe and be further used as a potential probiotic candidate for further clinical trials.
