RNA干扰作为植物抗病毒免疫的核心机制,其调控网络中SGS3(suppressor of gene silencing)基因在RDR6介导的双链RNA合成过程中扮演关键角色。为深入探究SGS3基因功能,对本氏烟草SGS3基因引入新型多靶点基因沉默系统——PTA(poly-tRNA-ami...RNA干扰作为植物抗病毒免疫的核心机制,其调控网络中SGS3(suppressor of gene silencing)基因在RDR6介导的双链RNA合成过程中扮演关键角色。为深入探究SGS3基因功能,对本氏烟草SGS3基因引入新型多靶点基因沉默系统——PTA(poly-tRNA-amiRNA)技术,并与传统单个amiRNA进行RNAi效率的系统性比较。首先针对SGS3基因的锌指(ZF)、XS和卷曲螺旋(coiled coil,CC)等三个结构域各设计合成一个特异性amiRNA,通过分子克隆技术构建pBI121-SGS3-amiRNA1/2/3重组表达载体。利用农杆菌介导的瞬时表达系统,对三个重组载体进行功能验证,结果显示amiRNA2对SGS3基因的沉默效率显著高于其他两个靶点。基于此,采用Golden Gate克隆技术,将上述三个amiRNA与三个tRNA串联组装,成功构建PTA-SGS3表达盒,并整合至pBI121植物表达载体。通过与携带绿色荧光蛋白标记的大豆花叶病毒侵染性克隆SMV-GFP共侵染本氏烟草,系统检测PTA系统的RNAi效率及抗病毒活性。RTqPCR分析表明,相较于单一amiRNA,PTA系统可使SGS3基因沉默效率提升44.8%,显著增强靶基因沉默效果。通过GFP荧光强度定量分析及DAB组织化学染色实验,发现经PTA表达盒侵染的本氏烟草中,SMV病毒积累量较单amiRNA处理组增加50.6%。展开更多
Inertial response control(IRC)makes variable-speed wind turbine generators(WTGs)provide short-term frequency support during contingencies by releasing the kinetic energy stored in wind turbine rotors.When frequency su...Inertial response control(IRC)makes variable-speed wind turbine generators(WTGs)provide short-term frequency support during contingencies by releasing the kinetic energy stored in wind turbine rotors.When frequency support is terminated,the rotor speed should be restored to optimum for maximum power point tracking(MPPT).Existing IRCs utilize rotor speed recovery(RSR)strategies with a consistent power reference function.However,under real turbulent wind with alternate gusts and lulls,the consistent power reference function may fail to restore rotor speed or cause unexpected secondary frequency drop(SFD).In this regard,this paper proposes a novel adaptive RSR strategy that not only restores rotor speed via the aerodynamic power enhanced by wind gusts,but also stabilizes the turbine at wind lulls by tracking a suboptimal power curve.Experiments on a wind power-integrated power system testbed validate the proposed RSR strategy can successfully restore rotor speed while attenuating SFD under turbulent wind.展开更多
We give a new result on the construction of K-frame generators for unitary systems by using the pseudo-inverses of involved operators,which provides an improvement to one known result on this topic.We also introduce t...We give a new result on the construction of K-frame generators for unitary systems by using the pseudo-inverses of involved operators,which provides an improvement to one known result on this topic.We also introduce the concept of K-woven generators for unitary systems,by means of which we investigate the weaving properties of K-frame generators for unitary systems.展开更多
This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational ...This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational efficiencies,and technological advancements.Linear generators,such as Induction,permanent magnet synchronous,and switched reluctance types,are highlighted for their direct conversion capability,eliminating the need for mechanical gearboxes.Rotary Induction generators,permanent magnet synchronous generators,and doubly-fed Induction generators are evaluated for their established engineering principles and integration with existing grid infrastructure.The paper discusses the historical development,environmental benefits,and ongoing advancements in wave energy technologies,emphasizing the increasing feasibility and scalability of wave energy as a renewable source.Through a comprehensive analysis,this review provides insights into the current state and future prospects of electrical generators in wave energy conversion,underscoring their potential to significantly reduce reliance on fossil fuels and mitigate environmental impacts.展开更多
An islanded microgrid exhibits poor transient power sharing between synchronous generators(SGs)and inverterinterfaced distributed generators(IIDGs).This large error of transient power-sharing may result in the overloa...An islanded microgrid exhibits poor transient power sharing between synchronous generators(SGs)and inverterinterfaced distributed generators(IIDGs).This large error of transient power-sharing may result in the overload of generators and a large deviation in frequency.In this paper,the mechanism that leads to poor transient power sharing is revealed.Then,a parameter design and a coordinated control strategy are proposed to improve transient power sharing.A coordinated enhanced power-sharing(EPS)control strategy is proposed for IIDGs,which prevents the overload of IIDGs in grid-forming mode and is compatible with the existing power sharing strategies.By using a hierarchical control structure,accurate transient power sharing is achieved without the knowledge of connecting impedance.The analysis results and the proposed control method are validated by simulation.展开更多
Harvesting energy from humid air to generate electricity represents a promising strategy for sustainable power generation.However,achieving high output and long-term stability in moisture-driven power generators(MPGs)...Harvesting energy from humid air to generate electricity represents a promising strategy for sustainable power generation.However,achieving high output and long-term stability in moisture-driven power generators(MPGs)remains a significant challenge.Here,we develop an efficient MPG by incorporating polymerized ionic liquid(PIL)and MXene through in-situ polymerization of cationic long chains within the MXene layers.This structural design enhances the hydrophilicity and ion dynamics,ensuring stable and sustained electrical output.A single MPG device delivers an open-circuit voltage of 0.65 V and a power density of 14.87 μW·cm^(-2),operating continuously for over 36 h.Surface characterization and quantum chemistry calculations elucidate that the mobile anions within the MPG move directionally under moisture gradients,while polymerized cations remain stationary,driving power generation.The MPG exhibits exceptional long-term stability,retaining about 80%of its initial voltage output after 30 days.Moreover,these MPGs demonstrate scalability for practical applications,capable of efficiently charging capacitors and powering LEDs through simple series-parallel configurations.This work offers a promising strategy to simultaneously enhance the performance and operational stability of MPGs,offering a sustainable solution for the direct conversion of low-grade thermal energy from moisture into clean electricity.展开更多
The petal-shaped distribution network has high power supply reliability.However,the closed-loop operation mode and the access of inverter-interfaced distributed generators(IIDGs)bring great challenges to the protectio...The petal-shaped distribution network has high power supply reliability.However,the closed-loop operation mode and the access of inverter-interfaced distributed generators(IIDGs)bring great challenges to the protection schemes.The current amplitude differential protection is an effective means to solve this problem,but the existing criterions rarely consider both sensitivity to high-resistance faults and low requirements for data synchronization.Therefore,the general variation laws of the amplitude difference between the current steady-state components at both terminals and the phase differences between current fault components at both terminals are revealed.For external faults,the steady-state-component current amplitude difference is around zero and the fault-component current phase difference is around 180◦.For internal faults,either the amplitude difference is large or the phase difference is small.Accordingly,a current differential protection scheme based on the pre-fault and postfault steady-state current is proposed.The amplitude and phase of current at both terminals of the protected line are required in the proposed scheme,which has low requirements for data synchronization.The simulation results show that the proposed protection scheme is not affected by the fault type,position,resistance and capacity of the IIDGs.It can also be applied to radial distribution networks with IIDGs.展开更多
To address the excessive complexity of monthly scheduling and the impact of uncertain net load on the chargeable energy of storage,a reduced time-period monthly scheduling model for thermal generators and energy stora...To address the excessive complexity of monthly scheduling and the impact of uncertain net load on the chargeable energy of storage,a reduced time-period monthly scheduling model for thermal generators and energy storage,incorporating daily minimum chargeable energy constraints,was developed.Firstly,considering the variations in the frequency of unit start-ups and shutdowns under different levels of net load fluctuation,a method was proposed to reduce decision time periods for unit start-up and shut-down operations.This approach,based on the characteristics of net load fluctuations,minimizes the decision variables of units,thereby simplifying the monthly schedulingmodel.Secondly,the relationship between energy storage charging and discharging power,net load,and the total maximum/minimum output of units was analyzed.Based on this,daily minimum chargeable energy constraints were established to ensure the energy storage system meets charging requirements under extreme net load scenarios.Finally,taking into account the operational costs of thermal generators and energy storage,load loss costs,and operational constraints,the reduced time-period monthly schedulingmodel was constructed.Case studies demonstrate that the proposedmethod effectively generates economical monthly operation plans for thermal generators and energy storage,significantly reduces model solution time,and satisfies the charging requirements of energy storage under extreme net load conditions.展开更多
背景全科医生的培养和发展始终是医学界关注的焦点,而各国对全科医生的培训模式和方法存在显著的差异。目的总结并揭示我国全科医生培养的热点、趋势以及挑战,同时对比国外做法,为我国全科医生的未来发展提供借鉴和指导。方法采用文献...背景全科医生的培养和发展始终是医学界关注的焦点,而各国对全科医生的培训模式和方法存在显著的差异。目的总结并揭示我国全科医生培养的热点、趋势以及挑战,同时对比国外做法,为我国全科医生的未来发展提供借鉴和指导。方法采用文献计量学的方法,以CiteSpace 6.1.R Advanced软件为工具,国内研究分析基于中国知网(CNKI),国外研究分析选取Web of Science核心数据库(WOSCC),对2013—2023年国内外全科医生培养的文献进行筛选与定量化分析,并归纳总结。结果我国全科医生教育的研究数量逐年增长;我国研究机构以首都医科大学全科医学与继续教育学院发文量最多,但各机构间的合作不紧密;国外主要发文国家为英国和澳大利亚;我国全科医生培养研究偏向于教育方法改革和服务方向优化,而同期国外全科医生培养的研究侧重于人工智能技术的应用和对精神心理疾病的关注。结论我国全科医生培养的研究活动增长显著,集中在教育方法改革和服务方向优化,但各研究机构间需加强合作。对比国外研究模式,我国需要更多地引入新兴技术(如人工智能),并提升对精神心理疾病的关注度,以促进全科医生培养的深度和广度发展。展开更多
基金supported by National Natural Science Foundation of China(51977111)the Six Talent Peaks High-level Talent Project in Jiangsu Province(XNY-025)the Special Fund of Jiangsu Province for Transformation of Scientific and Technological Achievements(BA2019045).
文摘Inertial response control(IRC)makes variable-speed wind turbine generators(WTGs)provide short-term frequency support during contingencies by releasing the kinetic energy stored in wind turbine rotors.When frequency support is terminated,the rotor speed should be restored to optimum for maximum power point tracking(MPPT).Existing IRCs utilize rotor speed recovery(RSR)strategies with a consistent power reference function.However,under real turbulent wind with alternate gusts and lulls,the consistent power reference function may fail to restore rotor speed or cause unexpected secondary frequency drop(SFD).In this regard,this paper proposes a novel adaptive RSR strategy that not only restores rotor speed via the aerodynamic power enhanced by wind gusts,but also stabilizes the turbine at wind lulls by tracking a suboptimal power curve.Experiments on a wind power-integrated power system testbed validate the proposed RSR strategy can successfully restore rotor speed while attenuating SFD under turbulent wind.
基金Supported by NSFC(Nos.12361028,11761057)Science Foundation of Jiangxi Education Department(Nos.GJJ202302,GJJ202303,GJJ202319).
文摘We give a new result on the construction of K-frame generators for unitary systems by using the pseudo-inverses of involved operators,which provides an improvement to one known result on this topic.We also introduce the concept of K-woven generators for unitary systems,by means of which we investigate the weaving properties of K-frame generators for unitary systems.
文摘This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational efficiencies,and technological advancements.Linear generators,such as Induction,permanent magnet synchronous,and switched reluctance types,are highlighted for their direct conversion capability,eliminating the need for mechanical gearboxes.Rotary Induction generators,permanent magnet synchronous generators,and doubly-fed Induction generators are evaluated for their established engineering principles and integration with existing grid infrastructure.The paper discusses the historical development,environmental benefits,and ongoing advancements in wave energy technologies,emphasizing the increasing feasibility and scalability of wave energy as a renewable source.Through a comprehensive analysis,this review provides insights into the current state and future prospects of electrical generators in wave energy conversion,underscoring their potential to significantly reduce reliance on fossil fuels and mitigate environmental impacts.
基金supported in part by National Natural Science Foundation of China under Grant 52125705in part by National Natural Science Foundation of China under Grant 52107194.
文摘An islanded microgrid exhibits poor transient power sharing between synchronous generators(SGs)and inverterinterfaced distributed generators(IIDGs).This large error of transient power-sharing may result in the overload of generators and a large deviation in frequency.In this paper,the mechanism that leads to poor transient power sharing is revealed.Then,a parameter design and a coordinated control strategy are proposed to improve transient power sharing.A coordinated enhanced power-sharing(EPS)control strategy is proposed for IIDGs,which prevents the overload of IIDGs in grid-forming mode and is compatible with the existing power sharing strategies.By using a hierarchical control structure,accurate transient power sharing is achieved without the knowledge of connecting impedance.The analysis results and the proposed control method are validated by simulation.
基金the National Natural Science Foundation of China(22278401 and 92163209)the ANSO Collaborative Research Program(ANSO-CR-KP-2022-12)+2 种基金Beijing Natural Science Foundation(2252011 and JQ22004)Beijing Nova Program(20230484478)for financial supportsupported by Public Computing Cloud,Renmin University of China.
文摘Harvesting energy from humid air to generate electricity represents a promising strategy for sustainable power generation.However,achieving high output and long-term stability in moisture-driven power generators(MPGs)remains a significant challenge.Here,we develop an efficient MPG by incorporating polymerized ionic liquid(PIL)and MXene through in-situ polymerization of cationic long chains within the MXene layers.This structural design enhances the hydrophilicity and ion dynamics,ensuring stable and sustained electrical output.A single MPG device delivers an open-circuit voltage of 0.65 V and a power density of 14.87 μW·cm^(-2),operating continuously for over 36 h.Surface characterization and quantum chemistry calculations elucidate that the mobile anions within the MPG move directionally under moisture gradients,while polymerized cations remain stationary,driving power generation.The MPG exhibits exceptional long-term stability,retaining about 80%of its initial voltage output after 30 days.Moreover,these MPGs demonstrate scalability for practical applications,capable of efficiently charging capacitors and powering LEDs through simple series-parallel configurations.This work offers a promising strategy to simultaneously enhance the performance and operational stability of MPGs,offering a sustainable solution for the direct conversion of low-grade thermal energy from moisture into clean electricity.
基金supported in part by Science and Technology Project of State Grid Corporation of China:Research on Key Protection Technologies for New-type Urban Distribution Network with Controllable Sources and Loads.
文摘The petal-shaped distribution network has high power supply reliability.However,the closed-loop operation mode and the access of inverter-interfaced distributed generators(IIDGs)bring great challenges to the protection schemes.The current amplitude differential protection is an effective means to solve this problem,but the existing criterions rarely consider both sensitivity to high-resistance faults and low requirements for data synchronization.Therefore,the general variation laws of the amplitude difference between the current steady-state components at both terminals and the phase differences between current fault components at both terminals are revealed.For external faults,the steady-state-component current amplitude difference is around zero and the fault-component current phase difference is around 180◦.For internal faults,either the amplitude difference is large or the phase difference is small.Accordingly,a current differential protection scheme based on the pre-fault and postfault steady-state current is proposed.The amplitude and phase of current at both terminals of the protected line are required in the proposed scheme,which has low requirements for data synchronization.The simulation results show that the proposed protection scheme is not affected by the fault type,position,resistance and capacity of the IIDGs.It can also be applied to radial distribution networks with IIDGs.
基金This study was supported by State Grid Corporation headquarters technology project(4000-202399368A-2-2-ZB).
文摘To address the excessive complexity of monthly scheduling and the impact of uncertain net load on the chargeable energy of storage,a reduced time-period monthly scheduling model for thermal generators and energy storage,incorporating daily minimum chargeable energy constraints,was developed.Firstly,considering the variations in the frequency of unit start-ups and shutdowns under different levels of net load fluctuation,a method was proposed to reduce decision time periods for unit start-up and shut-down operations.This approach,based on the characteristics of net load fluctuations,minimizes the decision variables of units,thereby simplifying the monthly schedulingmodel.Secondly,the relationship between energy storage charging and discharging power,net load,and the total maximum/minimum output of units was analyzed.Based on this,daily minimum chargeable energy constraints were established to ensure the energy storage system meets charging requirements under extreme net load scenarios.Finally,taking into account the operational costs of thermal generators and energy storage,load loss costs,and operational constraints,the reduced time-period monthly schedulingmodel was constructed.Case studies demonstrate that the proposedmethod effectively generates economical monthly operation plans for thermal generators and energy storage,significantly reduces model solution time,and satisfies the charging requirements of energy storage under extreme net load conditions.
文摘背景全科医生的培养和发展始终是医学界关注的焦点,而各国对全科医生的培训模式和方法存在显著的差异。目的总结并揭示我国全科医生培养的热点、趋势以及挑战,同时对比国外做法,为我国全科医生的未来发展提供借鉴和指导。方法采用文献计量学的方法,以CiteSpace 6.1.R Advanced软件为工具,国内研究分析基于中国知网(CNKI),国外研究分析选取Web of Science核心数据库(WOSCC),对2013—2023年国内外全科医生培养的文献进行筛选与定量化分析,并归纳总结。结果我国全科医生教育的研究数量逐年增长;我国研究机构以首都医科大学全科医学与继续教育学院发文量最多,但各机构间的合作不紧密;国外主要发文国家为英国和澳大利亚;我国全科医生培养研究偏向于教育方法改革和服务方向优化,而同期国外全科医生培养的研究侧重于人工智能技术的应用和对精神心理疾病的关注。结论我国全科医生培养的研究活动增长显著,集中在教育方法改革和服务方向优化,但各研究机构间需加强合作。对比国外研究模式,我国需要更多地引入新兴技术(如人工智能),并提升对精神心理疾病的关注度,以促进全科医生培养的深度和广度发展。
