Amphioxus or lancelets are regarded as a promising model animals for studying developmental mechanisms in chordates, and the evolution of vertebrate characters, because of their important phylogenetic position and the...Amphioxus or lancelets are regarded as a promising model animals for studying developmental mechanisms in chordates, and the evolution of vertebrate characters, because of their important phylogenetic position and their genomic and anatomical simplicity （Bertrand and Escriva, 2011; Holland and Yu, 2004）.展开更多
Hypertrophic chondrocytes(HCs)could transform into osteoblastic lineage cells while the pathophysiological implications of HC transformation remain largely unknown.Here,we generated a mouse line utilizing Col10a1-Cre ...Hypertrophic chondrocytes(HCs)could transform into osteoblastic lineage cells while the pathophysiological implications of HC transformation remain largely unknown.Here,we generated a mouse line utilizing Col10a1-Cre to induce DTA expression to genetically ablate HCs and their descendants.Col10a1-Cre;R26^(DTA/+)mice displayed dwarf phenotype,abnormal spongy bone,and significantly delayed drill-hole injuries healing,suggesting an indispensable role of HC lineage extension in bone growth and injury repair.Intriguingly,single-cell RNA sequencing analysis revealed the most significant loss of a cell cluster expressing multiple angiogenic factors(Pro-Angiogenic Descendants of HCs,PADs)among cells derived from Col10a1-Cre;R26^(DTA/+)and control femurs.In silico analysis of cell-cell communication supported Thrombospondin 4(THBS4)as a specific angiogenic factor mediating the crosstalk between PADs and vascular endothelial cells.Concordantly,analyses using immunostaining combined with tissue clearing revealed that PADs physically contacted with endothelial cells,whereas Col10a1-Cre;R26^(DTA/+)mice showed defective metaphyseal and cortical vessel formation and post-injury angiogenesis along with a significant loss of THBS4.Moreover,in vitro assays showed that supplying THBS4 was sufficient to promote proliferation and tube formation of endothelial cells and rescue defective angiogenesis of Col10a1-Cre;R26D TA/+metatarsal explants.Collectively,these findings demonstrate a critical role of PADs in bone growth and injury repair by secreting THBS4 to regulate angiogenesis.展开更多
Applying the Fourier transform to the wave equations of elastic medium at its surface a laser pulse line source is acted, the integral representations of solutions are obtained. Displacement waveforms are calculated n...Applying the Fourier transform to the wave equations of elastic medium at its surface a laser pulse line source is acted, the integral representations of solutions are obtained. Displacement waveforms are calculated numerically by using double FFT. The calculated results include the displacements of the elastic half space of Aluminum medium, and epicenter and off-epicenter of an Aluminum plate. The two exciting sources of thermoelastic and ablating generation are considered respectively. The experiment was made on the Aluminum medium with a Nd:YAG laser and the normal displacement signals are detected by a laser interferometer. The numerical results are quite in agreement with experiments.展开更多
The generation of genetically modified lines in model and economically important species typically requires months or even years.Developing strategies to accelerate this process thus represents a critical need in the ...The generation of genetically modified lines in model and economically important species typically requires months or even years.Developing strategies to accelerate this process thus represents a critical need in the field.Transplantation of allogeneic gonadal primordia into near-mature recipients offers a promising route to rapid gamete production.However,a major obstacle lies in overcoming the immune rejection of donor tissues.展开更多
基金supported by the grants from National Natural Science Foundation of China (Nos.31372188,31471986 and 31672246)Fundamental Research Funds for the Central Universities, China (No.20720160056)
文摘Amphioxus or lancelets are regarded as a promising model animals for studying developmental mechanisms in chordates, and the evolution of vertebrate characters, because of their important phylogenetic position and their genomic and anatomical simplicity （Bertrand and Escriva, 2011; Holland and Yu, 2004）.
基金financial support from the National Natural Science Foundation of China(grants 82394442,82422043,82272442)the Key Industrial Chain Program of Shaanxi,China(No.2022ZDLSF02-12)。
文摘Hypertrophic chondrocytes(HCs)could transform into osteoblastic lineage cells while the pathophysiological implications of HC transformation remain largely unknown.Here,we generated a mouse line utilizing Col10a1-Cre to induce DTA expression to genetically ablate HCs and their descendants.Col10a1-Cre;R26^(DTA/+)mice displayed dwarf phenotype,abnormal spongy bone,and significantly delayed drill-hole injuries healing,suggesting an indispensable role of HC lineage extension in bone growth and injury repair.Intriguingly,single-cell RNA sequencing analysis revealed the most significant loss of a cell cluster expressing multiple angiogenic factors(Pro-Angiogenic Descendants of HCs,PADs)among cells derived from Col10a1-Cre;R26^(DTA/+)and control femurs.In silico analysis of cell-cell communication supported Thrombospondin 4(THBS4)as a specific angiogenic factor mediating the crosstalk between PADs and vascular endothelial cells.Concordantly,analyses using immunostaining combined with tissue clearing revealed that PADs physically contacted with endothelial cells,whereas Col10a1-Cre;R26^(DTA/+)mice showed defective metaphyseal and cortical vessel formation and post-injury angiogenesis along with a significant loss of THBS4.Moreover,in vitro assays showed that supplying THBS4 was sufficient to promote proliferation and tube formation of endothelial cells and rescue defective angiogenesis of Col10a1-Cre;R26D TA/+metatarsal explants.Collectively,these findings demonstrate a critical role of PADs in bone growth and injury repair by secreting THBS4 to regulate angiogenesis.
基金This work is supported by the National Natural Science Foundation of ChinaState key Lab.of Information Function Material of
文摘Applying the Fourier transform to the wave equations of elastic medium at its surface a laser pulse line source is acted, the integral representations of solutions are obtained. Displacement waveforms are calculated numerically by using double FFT. The calculated results include the displacements of the elastic half space of Aluminum medium, and epicenter and off-epicenter of an Aluminum plate. The two exciting sources of thermoelastic and ablating generation are considered respectively. The experiment was made on the Aluminum medium with a Nd:YAG laser and the normal displacement signals are detected by a laser interferometer. The numerical results are quite in agreement with experiments.
文摘The generation of genetically modified lines in model and economically important species typically requires months or even years.Developing strategies to accelerate this process thus represents a critical need in the field.Transplantation of allogeneic gonadal primordia into near-mature recipients offers a promising route to rapid gamete production.However,a major obstacle lies in overcoming the immune rejection of donor tissues.
