Background and Objective:Recent studies proved that P21-activated kinase 1(PAK1) is highly expressed in many kinds of tumor and plays an important role in genesis,development,and metastasis of tumor.We aimed to detect...Background and Objective:Recent studies proved that P21-activated kinase 1(PAK1) is highly expressed in many kinds of tumor and plays an important role in genesis,development,and metastasis of tumor.We aimed to detect the expression of PAK1 in gastric carcinoma and to analyze its relationship with clinicopathological features and prognosis of gastric carcinoma.Met hods:Tissue microarray and immunohistochemical staining were performed to detect PAK1 in paraffin specimens of 189 gastric carcinomas,54 paracancer tissues,40 lymph nodes and 30 healthy tissues.Clinicopathologic features and follow-up data of the patients were analyzed by the χ2 test and the Kaplan-Meier method.Result s:Positive rate of PAK1 was 73.0% in gastric carcinoma,57.4% in paracancer tissues and 23.3% in healthy controls(χ2 = 29.364,P < 0.05).Expression of PAK1 was significantly correlated with tumor size,tumor differentiation,lymph node metastasis,Lauren classification and invasive depth(all P < 0.05).The positive rate of PAK1 was significantly higher in primary gastric carcinomas than in metastatic lymph nodes(75.0% vs.52.5%,χ2 = 4.381,P < 0.05).Survival analysis using the Kaplan-Meier method showed that the expression of PAK1 was a predictor for poor prognosis of the patients with gastric carcinoma(χ2 = 6.857,P < 0.01).Conclusions:Expression of PAK1 is an early molecular event in the tumorigenesis of gastric carcinoma.It is also closely correlated the development of gastric carcinoma and the patients' prognosis.展开更多
Objective： To investigate the effect of Musa sapientum L. （MS） bark juice in diabetic gastroparesis and its effect on pharrnacokinetic of metformin （MET）. Methods： Diabetes was induced in rats by administering a...Objective： To investigate the effect of Musa sapientum L. （MS） bark juice in diabetic gastroparesis and its effect on pharrnacokinetic of metformin （MET）. Methods： Diabetes was induced in rats by administering alloxan （120 mg/kg） saline solution and maintained for 8 week. All the 18 Sprague-Dawley rats were divided into three groups （n=6 in each group）： normal control, diabetic control and MS bark juice. Assessment of diabetes was done by glucose oxidase-peroxidase method on the 3rd day of alloxan administration. The effects of MS bark juice （100 mL/kg） on gastric emptying time, intestinal transit time, contractility of fundus and pylorus as well as gastdc acid secretion in chronic diabetic rats were observed after 8 weeks of aUoxan administration. The effect of MS bark juice on the pharmacokinetic of orally administered single dose of MET （350 mg/kg） was evaluated on the 57th day of protocol. Any drugs that may reduce the blood glucose level or influence the fibrinolytic system were not used in this study. Results： The MS bark juice significantly reduced the blood glucose level in the diabetic rats （P〈0.01）. There was significant decrease in the pylorus motility and increase in the gastric emptying time, intestinal transit time, contractility of fundus, gastric acid secretion in the MS bark juice treated group （P〈0.01）. There was significant decrease in the time at which drug at a maximum concentration, haft life of drug and increase in the maximum concentration of drug in the plasma of MET in MS bark juice treated group as compared to diabetic control group （P〈0.01）. Conclusion： MS bark juice effectively manages diabetic gastroparesis and thereby improves the bioavailabilty of MET when administered with MS bark iuice.展开更多
基金National Natural Science Foundation of China (No. 30872478)
文摘Background and Objective:Recent studies proved that P21-activated kinase 1(PAK1) is highly expressed in many kinds of tumor and plays an important role in genesis,development,and metastasis of tumor.We aimed to detect the expression of PAK1 in gastric carcinoma and to analyze its relationship with clinicopathological features and prognosis of gastric carcinoma.Met hods:Tissue microarray and immunohistochemical staining were performed to detect PAK1 in paraffin specimens of 189 gastric carcinomas,54 paracancer tissues,40 lymph nodes and 30 healthy tissues.Clinicopathologic features and follow-up data of the patients were analyzed by the χ2 test and the Kaplan-Meier method.Result s:Positive rate of PAK1 was 73.0% in gastric carcinoma,57.4% in paracancer tissues and 23.3% in healthy controls(χ2 = 29.364,P < 0.05).Expression of PAK1 was significantly correlated with tumor size,tumor differentiation,lymph node metastasis,Lauren classification and invasive depth(all P < 0.05).The positive rate of PAK1 was significantly higher in primary gastric carcinomas than in metastatic lymph nodes(75.0% vs.52.5%,χ2 = 4.381,P < 0.05).Survival analysis using the Kaplan-Meier method showed that the expression of PAK1 was a predictor for poor prognosis of the patients with gastric carcinoma(χ2 = 6.857,P < 0.01).Conclusions:Expression of PAK1 is an early molecular event in the tumorigenesis of gastric carcinoma.It is also closely correlated the development of gastric carcinoma and the patients' prognosis.
文摘Objective： To investigate the effect of Musa sapientum L. （MS） bark juice in diabetic gastroparesis and its effect on pharrnacokinetic of metformin （MET）. Methods： Diabetes was induced in rats by administering alloxan （120 mg/kg） saline solution and maintained for 8 week. All the 18 Sprague-Dawley rats were divided into three groups （n=6 in each group）： normal control, diabetic control and MS bark juice. Assessment of diabetes was done by glucose oxidase-peroxidase method on the 3rd day of alloxan administration. The effects of MS bark juice （100 mL/kg） on gastric emptying time, intestinal transit time, contractility of fundus and pylorus as well as gastdc acid secretion in chronic diabetic rats were observed after 8 weeks of aUoxan administration. The effect of MS bark juice on the pharmacokinetic of orally administered single dose of MET （350 mg/kg） was evaluated on the 57th day of protocol. Any drugs that may reduce the blood glucose level or influence the fibrinolytic system were not used in this study. Results： The MS bark juice significantly reduced the blood glucose level in the diabetic rats （P〈0.01）. There was significant decrease in the pylorus motility and increase in the gastric emptying time, intestinal transit time, contractility of fundus, gastric acid secretion in the MS bark juice treated group （P〈0.01）. There was significant decrease in the time at which drug at a maximum concentration, haft life of drug and increase in the maximum concentration of drug in the plasma of MET in MS bark juice treated group as compared to diabetic control group （P〈0.01）. Conclusion： MS bark juice effectively manages diabetic gastroparesis and thereby improves the bioavailabilty of MET when administered with MS bark iuice.
