Objective:To explore the target gene and mechanism of effective components of Renshen-Gansong in the treatment of paroxysmal atrial fibrillation based on network pharmacology.Methods:The chemical constituents of Rensh...Objective:To explore the target gene and mechanism of effective components of Renshen-Gansong in the treatment of paroxysmal atrial fibrillation based on network pharmacology.Methods:The chemical constituents of Renshen-Gansong drug pairs were searched by TCMSP traditional Chinese medicine database.The potentially effective components were screened under the conditions of bioavailability(OB)≥30%and drug-like(DL)≥0.18,and the potential targets were predicted by TCMSP database.The human gene name corresponding to the potential target was found by Uniprot database,and the disease target of paroxysmal atrial fibrillation was searched by Genecards database,the intersection target was mapped with the potential target of drug pair,the Wayne diagram was drawn,and the disease-drug-component-target network map was constructed by Cytoscape3.7.2.The PPI protein interaction network map was constructed by STRING database to select the core targets,and finally GO analysis and KEGG analysis were carried out.Results:A total of 28 active components and 45 effective targets were obtained.GO enrichment analysis showed that the main pathways were neurotransmitter receptor activity,ion gated channel activity,passive transmembrane transporter activity,G protein coupled neurotransmitter receptor activity and so on.KEGG pathway enrichment analysis showed that the main pathways were IL-17 signal pathway,calcium signal pathway,TNF signal pathway and so on.Conclusion:Renshen-Gansong has a synergistic effect on the treatment of paroxysmal atrial fibrillation through multi-targets,multi-pathways and multiple signal pathways,which provides a basis for further study of drug mechanism and clinical guidance.展开更多
Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to veri...Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.展开更多
目的:运用文献计量学软件VOSviewer和CiteSpace对甘松的研究热点及发展趋势进行可视化分析。方法:以甘松为主题词,系统检索中国知网(CNKI)和Web of Science(WOS)核心合集数据库中的相关研究文献,检索时间自建库至2024年6月30日,借助VOSv...目的:运用文献计量学软件VOSviewer和CiteSpace对甘松的研究热点及发展趋势进行可视化分析。方法:以甘松为主题词,系统检索中国知网(CNKI)和Web of Science(WOS)核心合集数据库中的相关研究文献,检索时间自建库至2024年6月30日,借助VOSviewer、CiteSpace、Excel软件分别对甘松中、英文文献的发文量、核心作者群、机构及国家分布、期刊和被引文献、关键词特征等进行分析,并绘制可视化知识图谱。结果:共纳入文献1108篇,涵盖中文文献898篇,英文文献210篇。甘松年发文量总体呈波动增长趋势,共有37个国家参与甘松的研究,其中以中国为主。中文文献发文量最多的作者是南一和袁玲,英文文献发文量最多的作者是Wu Honghua;北京中医药大学和中国科学院发文量分别居于中、英文文献发文量首位,各研究机构及团队间的交流合作有待进一步深化。中、英文关键词共现、聚类分析结果表明,当前甘松研究主要集中于化学成分、药理作用、临床应用和机制研究等方向。其中,中文文献侧重甘松的心脏保护作用及复方配伍研究,英文文献关注甘松的药物活性成分及神经药理机制。结论:甘松研究目前仍处于上升阶段,结合网络药理学及组学技术等的多靶点机制研究、药代动力学及毒理学研究、新产品开发、人工栽培技术优化及替代资源的挖掘等是甘松未来研究的新方向。展开更多
基金National Science and Technology Major Special Project on Major New Drug Innovation(No.2017ZX09301003)。
文摘Objective:To explore the target gene and mechanism of effective components of Renshen-Gansong in the treatment of paroxysmal atrial fibrillation based on network pharmacology.Methods:The chemical constituents of Renshen-Gansong drug pairs were searched by TCMSP traditional Chinese medicine database.The potentially effective components were screened under the conditions of bioavailability(OB)≥30%and drug-like(DL)≥0.18,and the potential targets were predicted by TCMSP database.The human gene name corresponding to the potential target was found by Uniprot database,and the disease target of paroxysmal atrial fibrillation was searched by Genecards database,the intersection target was mapped with the potential target of drug pair,the Wayne diagram was drawn,and the disease-drug-component-target network map was constructed by Cytoscape3.7.2.The PPI protein interaction network map was constructed by STRING database to select the core targets,and finally GO analysis and KEGG analysis were carried out.Results:A total of 28 active components and 45 effective targets were obtained.GO enrichment analysis showed that the main pathways were neurotransmitter receptor activity,ion gated channel activity,passive transmembrane transporter activity,G protein coupled neurotransmitter receptor activity and so on.KEGG pathway enrichment analysis showed that the main pathways were IL-17 signal pathway,calcium signal pathway,TNF signal pathway and so on.Conclusion:Renshen-Gansong has a synergistic effect on the treatment of paroxysmal atrial fibrillation through multi-targets,multi-pathways and multiple signal pathways,which provides a basis for further study of drug mechanism and clinical guidance.
基金National Natural Science Foundation of People's Republic of China(No.30660060)
文摘Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.
文摘目的:运用文献计量学软件VOSviewer和CiteSpace对甘松的研究热点及发展趋势进行可视化分析。方法:以甘松为主题词,系统检索中国知网(CNKI)和Web of Science(WOS)核心合集数据库中的相关研究文献,检索时间自建库至2024年6月30日,借助VOSviewer、CiteSpace、Excel软件分别对甘松中、英文文献的发文量、核心作者群、机构及国家分布、期刊和被引文献、关键词特征等进行分析,并绘制可视化知识图谱。结果:共纳入文献1108篇,涵盖中文文献898篇,英文文献210篇。甘松年发文量总体呈波动增长趋势,共有37个国家参与甘松的研究,其中以中国为主。中文文献发文量最多的作者是南一和袁玲,英文文献发文量最多的作者是Wu Honghua;北京中医药大学和中国科学院发文量分别居于中、英文文献发文量首位,各研究机构及团队间的交流合作有待进一步深化。中、英文关键词共现、聚类分析结果表明,当前甘松研究主要集中于化学成分、药理作用、临床应用和机制研究等方向。其中,中文文献侧重甘松的心脏保护作用及复方配伍研究,英文文献关注甘松的药物活性成分及神经药理机制。结论:甘松研究目前仍处于上升阶段,结合网络药理学及组学技术等的多靶点机制研究、药代动力学及毒理学研究、新产品开发、人工栽培技术优化及替代资源的挖掘等是甘松未来研究的新方向。
