The coupling effects of axial pre-stress, temperature and magnetic field on magne- tostrictive strain and magnetization as well as Young＇s modulus ofa Terfenol-D （Tbo.3Dyo.rFei.93） rod are tested to give a good und...The coupling effects of axial pre-stress, temperature and magnetic field on magne- tostrictive strain and magnetization as well as Young＇s modulus ofa Terfenol-D （Tbo.3Dyo.rFei.93） rod are tested to give a good understanding of magneto-thermal-mecha- nical characteristics of giant magnetostrictive materials. Results show that magneto-thermo-mechanical coupling of giant magnetostrictive materials is very strong; and the influences of pre-stress and temperature on magnetostrictive strain and Young＇s modulus vary with the intensity of magnetic field.展开更多
Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the xenografts.To overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigen...Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the xenografts.To overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigens[Gal,Neu5 Gc,and Sd(a)]present on pig cells and tissues.Triple gene(GGTAI,CMAH,and β4 GalNT2)knockout(TKO)pigs were produced in our laboratory by CRISPR-Cas9 targeting.To investigate the antigenicity reduction in the TKO pigs,the expression levels of these three xenoantigens in the cornea,heart,liver,spleen,lung,kidney,and pancreas tissues were examined.The level of human IgG/IgM binding to those tissues was also investigated,with wildtype pig tissues as control.The results showed that aGal,Neu5 Gc,and Sd(a)were markedly positive in all the examined tissues in wildtype pigs but barely detected in TKO pigs.Compared to wildtype pigs,the liver,spleen,and pancreas of TKO pigs showed comparable levels of human IgG and IgM binding,whereas corneas,heart,lung,and kidney of TKO pigs exhibited significantly reduced human IgG and IgM binding.These results indicate that the antigenicity of TKO pig is significantly reduced and the remaining xenoantigens on porcine tissues can be eliminated via a gene targeting approach.展开更多
基金Project supported by the National Natural Science Foundation of China (No.90405005)the Ph.D Fund of the Ministry of Education of China (No.20050730016).
文摘The coupling effects of axial pre-stress, temperature and magnetic field on magne- tostrictive strain and magnetization as well as Young＇s modulus ofa Terfenol-D （Tbo.3Dyo.rFei.93） rod are tested to give a good understanding of magneto-thermal-mecha- nical characteristics of giant magnetostrictive materials. Results show that magneto-thermo-mechanical coupling of giant magnetostrictive materials is very strong; and the influences of pre-stress and temperature on magnetostrictive strain and Young＇s modulus vary with the intensity of magnetic field.
基金supported by grants from the National Natural Science Foundation of China(81570402&31701283)the National Key R&D Program of China(2017YFC1103701&2017YFC1103702)+3 种基金the Jiangsu Key Laboratory of Xenotransplantation(BM2012116)the Sanming Project of Medicine in Shenzhenthe Fund for High Level Medical Discipline Construction of Shenzhen(2016031638)the Shenzhen Foundation of Science and Technology(JCYJ20160229204849975&GCZX2015043017281705)
文摘Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the xenografts.To overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigens[Gal,Neu5 Gc,and Sd(a)]present on pig cells and tissues.Triple gene(GGTAI,CMAH,and β4 GalNT2)knockout(TKO)pigs were produced in our laboratory by CRISPR-Cas9 targeting.To investigate the antigenicity reduction in the TKO pigs,the expression levels of these three xenoantigens in the cornea,heart,liver,spleen,lung,kidney,and pancreas tissues were examined.The level of human IgG/IgM binding to those tissues was also investigated,with wildtype pig tissues as control.The results showed that aGal,Neu5 Gc,and Sd(a)were markedly positive in all the examined tissues in wildtype pigs but barely detected in TKO pigs.Compared to wildtype pigs,the liver,spleen,and pancreas of TKO pigs showed comparable levels of human IgG and IgM binding,whereas corneas,heart,lung,and kidney of TKO pigs exhibited significantly reduced human IgG and IgM binding.These results indicate that the antigenicity of TKO pig is significantly reduced and the remaining xenoantigens on porcine tissues can be eliminated via a gene targeting approach.
