Human cytomegalovirus is an important pathogen which widely infects human.In this study,an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore t...Human cytomegalovirus is an important pathogen which widely infects human.In this study,an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly,the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot.The maxiprepared plasmid pVAX1/gB680+pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined.Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells,and could induce antibodies and cellular immune responses in BALB/c mice.展开更多
文摘Human cytomegalovirus is an important pathogen which widely infects human.In this study,an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly,the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot.The maxiprepared plasmid pVAX1/gB680+pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined.Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells,and could induce antibodies and cellular immune responses in BALB/c mice.
