Lysozyme(EC3.2.1.17)plays an important role in the immune response;as a nonspecific immune factor,it can resist causative agents.Lysozyme can be divided into c-type and g-type in fish.In a previous study,through genom...Lysozyme(EC3.2.1.17)plays an important role in the immune response;as a nonspecific immune factor,it can resist causative agents.Lysozyme can be divided into c-type and g-type in fish.In a previous study,through genome-wide association analysis,the g-type lysozyme gene,which is named NaLyg in yellow drum(Nibea albiflora),was found to be a key candidate gene for disease resistance in response to Vibrio harveyi infection.The cDNA of NaLyg was 1025 bp,including four exons and three introns,and its open reading frame(ORF)had a full-length of 582 bp,encoding 193 amino acids.NaLyg was found to be conserved during evolution through bioinformatic analyses.The NaLyg protein possessed a sugar binding domain and three catalytic sites,including Glu71,Asp84 and Asp101.Quantitative qRT-PCR results confirmed that NaLyg gene mRNA was visibly increased after V.harveyi infection.The NaLyg protein purified by prokaryotic expression killed some gram-negative bacterial pathogens by inducing cell wall destruction,including V.harveyi,Aeromonas hydrophila and Edwardsiella tarda.Moreover,the NaLyg protein killed two gram-positive bacteria,Bacillus subtilis and Staphylococcus aureus.Taken together,the experimental results suggested that the NaLyg protein of N.albiflora played an important role in fighting bacterial infections.展开更多
Conventional squeeze film dampers have numerous challenges including lock up,bistable response and incoordinate precession.In order to resolve these nonlinear problems,a novel G-type integral squeeze film damper(GISFD...Conventional squeeze film dampers have numerous challenges including lock up,bistable response and incoordinate precession.In order to resolve these nonlinear problems,a novel G-type integral squeeze film damper(GISFD)is proposed in this research.The experimental test rig is provided to investigate the rotor system with an unbalanced single disk.Numerical simulation results show that the structural design of GISFD is reasonable,which can ensure its safe and stable operation.The influence of different support stiffnesses on the first-order speed of the rotor system is analyzed.Experimental results show that GISFD can effectively suppress the unbalanced response vibration of the rotor.In a certain range,it is found that the suppression effect of GISFD increases with the increase in the kinematic viscosity of the damping fluid.When the silicone oil with kinematic viscosity coefficients v=30.0 cm^(2)/s is employed,the vibration reduction of GISFD is approximately 71.51%.Furthermore,the experimental results show that with the increase of the unbalance,there is a linear relationship between the unbalance and the corresponding amplitude of the unbalanced response.It is concluded that GISFD has excellent linear damping characteristics and reduces the sensitivity of the rotor system to the unbalanced mass.展开更多
This paper studies dispersion of a G-type earthquake wave under the influence of a suppressed rigid boundary. Inside the Earth, the density and rigidity of the crustal layer and the mantle of the Earth vary exponentia...This paper studies dispersion of a G-type earthquake wave under the influence of a suppressed rigid boundary. Inside the Earth, the density and rigidity of the crustal layer and the mantle of the Earth vary exponentially and periodically along the depth. The displacements of the wave are found in the individual medium followed by a dispersion equation using a suitable analytic approach and a boundary condition. The prominent effect of inhomogeneity contained in the media, the rigid boundary plane, and the initial stress on the phase and group velocities is shown graphically.展开更多
To relax convexity assumptions imposed on the functions in theorems on sufficient conditions and duality,new concepts of generalized dI-G-type Ⅰ invexity were introduced for nondifferentiable multiobjective programmi...To relax convexity assumptions imposed on the functions in theorems on sufficient conditions and duality,new concepts of generalized dI-G-type Ⅰ invexity were introduced for nondifferentiable multiobjective programming problems.Based upon these generalized invexity,G-Fritz-John （G-F-J） and G-Karnsh-Kuhn-Tucker （G-K-K-T） types sufficient optimality conditions were established for a feasible solution to be an efficient solution.Moreover,weak and strict duality results were derived for a G-Mond-Weir type dual under various types of generalized dI-G-type Ⅰ invexity assumptions.展开更多
The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray ...The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is differentially expressed between two Gossypium hirsutum lines-Texas Marker-1 (TM-1) and Chromosome Substitution Line CS-B25. The CS-B25 line has chromosome 25 from G. barbadense substituted into TM-1. The complete nucleotide sequences of GhRING2 along with its 5’-flanking region were obtained by genomic walking. GhRING2 was highly expressed in elongating fiber, and GUS expression directed by the GhRING2 promoter was found in hypocotyls and young stems of transgenic Arabidopsis plants. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERMAL FACTOR1 (GhPDF1) protein. GhPDF1 was expressed preferentially in immature ovules and fiber initials, and the GhPDF1 gene had been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays further confirmed the interaction between GhRING2 and GhPDF1. The expression and protein interaction data indicate that GhRING2 may be involved in the turnover of GhPDF1 and participation in the transition from initiation to elongation stages during fiber development. Our data strongly suggest that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. The nucleotide sequence data of GhRING2 in this article have been submitted to the Gen Bank Nucleotide Sequence Data Bases under the accession number BankIt 1,742,008 SeqKM 108,000.展开更多
基金supported by the National Natural Science Foundation of China(No.32072969)the National Key R&D Program of China(No.2022YFD2401002)+1 种基金the Natural Science Foundation of Fujian Province(No.2022 J01325)the Open Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment(No.Z822280).
文摘Lysozyme(EC3.2.1.17)plays an important role in the immune response;as a nonspecific immune factor,it can resist causative agents.Lysozyme can be divided into c-type and g-type in fish.In a previous study,through genome-wide association analysis,the g-type lysozyme gene,which is named NaLyg in yellow drum(Nibea albiflora),was found to be a key candidate gene for disease resistance in response to Vibrio harveyi infection.The cDNA of NaLyg was 1025 bp,including four exons and three introns,and its open reading frame(ORF)had a full-length of 582 bp,encoding 193 amino acids.NaLyg was found to be conserved during evolution through bioinformatic analyses.The NaLyg protein possessed a sugar binding domain and three catalytic sites,including Glu71,Asp84 and Asp101.Quantitative qRT-PCR results confirmed that NaLyg gene mRNA was visibly increased after V.harveyi infection.The NaLyg protein purified by prokaryotic expression killed some gram-negative bacterial pathogens by inducing cell wall destruction,including V.harveyi,Aeromonas hydrophila and Edwardsiella tarda.Moreover,the NaLyg protein killed two gram-positive bacteria,Bacillus subtilis and Staphylococcus aureus.Taken together,the experimental results suggested that the NaLyg protein of N.albiflora played an important role in fighting bacterial infections.
基金Supported by the National Science and Technology Major Project(No.2017-Ⅳ-0010-0047)the Fundamental Research Funds for the Central Universities(No.JD2003)。
文摘Conventional squeeze film dampers have numerous challenges including lock up,bistable response and incoordinate precession.In order to resolve these nonlinear problems,a novel G-type integral squeeze film damper(GISFD)is proposed in this research.The experimental test rig is provided to investigate the rotor system with an unbalanced single disk.Numerical simulation results show that the structural design of GISFD is reasonable,which can ensure its safe and stable operation.The influence of different support stiffnesses on the first-order speed of the rotor system is analyzed.Experimental results show that GISFD can effectively suppress the unbalanced response vibration of the rotor.In a certain range,it is found that the suppression effect of GISFD increases with the increase in the kinematic viscosity of the damping fluid.When the silicone oil with kinematic viscosity coefficients v=30.0 cm^(2)/s is employed,the vibration reduction of GISFD is approximately 71.51%.Furthermore,the experimental results show that with the increase of the unbalance,there is a linear relationship between the unbalance and the corresponding amplitude of the unbalanced response.It is concluded that GISFD has excellent linear damping characteristics and reduces the sensitivity of the rotor system to the unbalanced mass.
基金supported by the National Natural Science Foundation of China(No.11471087)the China Postdoctoral Science Foundation(No.2013M540270)+2 种基金the Heilongjiang Postdoctoral Foundation(No.LBH-Z13056)the Support Plan for the Young College Academic Backbone of Heilongjiang Province(No.1252G020)the Fundamental Research Funds for the Central Universities
文摘This paper studies dispersion of a G-type earthquake wave under the influence of a suppressed rigid boundary. Inside the Earth, the density and rigidity of the crustal layer and the mantle of the Earth vary exponentially and periodically along the depth. The displacements of the wave are found in the individual medium followed by a dispersion equation using a suitable analytic approach and a boundary condition. The prominent effect of inhomogeneity contained in the media, the rigid boundary plane, and the initial stress on the phase and group velocities is shown graphically.
基金National Natural Science Foundation of China(No.11071110)
文摘To relax convexity assumptions imposed on the functions in theorems on sufficient conditions and duality,new concepts of generalized dI-G-type Ⅰ invexity were introduced for nondifferentiable multiobjective programming problems.Based upon these generalized invexity,G-Fritz-John （G-F-J） and G-Karnsh-Kuhn-Tucker （G-K-K-T） types sufficient optimality conditions were established for a feasible solution to be an efficient solution.Moreover,weak and strict duality results were derived for a G-Mond-Weir type dual under various types of generalized dI-G-type Ⅰ invexity assumptions.
文摘The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is differentially expressed between two Gossypium hirsutum lines-Texas Marker-1 (TM-1) and Chromosome Substitution Line CS-B25. The CS-B25 line has chromosome 25 from G. barbadense substituted into TM-1. The complete nucleotide sequences of GhRING2 along with its 5’-flanking region were obtained by genomic walking. GhRING2 was highly expressed in elongating fiber, and GUS expression directed by the GhRING2 promoter was found in hypocotyls and young stems of transgenic Arabidopsis plants. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERMAL FACTOR1 (GhPDF1) protein. GhPDF1 was expressed preferentially in immature ovules and fiber initials, and the GhPDF1 gene had been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays further confirmed the interaction between GhRING2 and GhPDF1. The expression and protein interaction data indicate that GhRING2 may be involved in the turnover of GhPDF1 and participation in the transition from initiation to elongation stages during fiber development. Our data strongly suggest that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. The nucleotide sequence data of GhRING2 in this article have been submitted to the Gen Bank Nucleotide Sequence Data Bases under the accession number BankIt 1,742,008 SeqKM 108,000.
