A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the p...A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the pulses efficiently.Other PDW information such as rise time,carrier frequency,pulse width,modulation on pulse,fall time and direction of arrival are not required.To identify the valid PRIs in a set of interleaved pulses,an innovative modification of the DBSCAN algorithm is introduced which is accurate and easy to implement.The proposed method determines valid PRIs more accurately and neglects the spurious ones more efficiently as compared to the classical histogram based algorithms such as SDIF.Furthermore,without specifying any input parameter,the proposed method can deinterleave radar pulses while up to 30%jitter is present in the associated PRI.The accuracy and efficiency of the proposed method are verified by computer simulations and real data results.Experimental simulations are based on different real and operational scenarios where the presence of missing and spurious pulses are also considered.So,the simulation results can be of practical significance.展开更多
The nucleotide (base) sequence of the genome might reflect biological information beyond the coding sequences. The appearance frequencies of successive base sequences (key sequences) were calculated for entire genomes...The nucleotide (base) sequence of the genome might reflect biological information beyond the coding sequences. The appearance frequencies of successive base sequences (key sequences) were calculated for entire genomes. Based on the appearance frequency of the key sequences of the genome, any DNA sequences on the genome could be expressed as a sequence spectrum with the adjoining base sequences, which could be used to study the corresponding biological phenomena. In this paper, we used 64 successive three- base sequences (triplets) as the key sequences, and determined and compared the spectra of specific genes to the chromosome, or specific genes to tRNA genes in Saccharomyces cerevisiae, Schizosaccharomyces pombe and Escherichia coli. Based on these analyses, a gene and its corresponding position on the chromosome showed highly similar spectra with the same fold enlargement (approximately 400-fold) in the S. cerevisiae, S. pombe and E. coli genomes. In addition, the homologous structure of genes that encode proteins was also observed with appropriate tRNA gene(s) in the genome. This analytical method might faithfully reflect the encoded biological information, that is, the conservation of the base sequences was to make sense the conservation of the translated amino acids sequence in the coding region, and might be universally applicable to other genomes, even those that consisted of multiple chromosomes.展开更多
A novel diagrammtic method is proposed to show the angular distribution of bases of human protein sequences. Using this method, the distribution sphere[1-4] is divided into four regions with same volume. The picture i...A novel diagrammtic method is proposed to show the angular distribution of bases of human protein sequences. Using this method, the distribution sphere[1-4] is divided into four regions with same volume. The picture is clearer and more intuitive than that in [1] .A rule on the angular distribution of the representative points of bases of protein sequences is given. Besides, in 300 representative pointS of human protein sequence samples we find that there are three (not only one) points outside the sphere.展开更多
In this paper, we study the invertibility of sequences consisting of finitely many bounded linear operators from a Hilbert space to others. We show that a sequence of operators is left invertible if and only if it is ...In this paper, we study the invertibility of sequences consisting of finitely many bounded linear operators from a Hilbert space to others. We show that a sequence of operators is left invertible if and only if it is a g-frame. Therefore, our result connects the invertibility of operator sequences with frame theory.展开更多
The satellite-based automatic identification system (AIS) receiver has to encounter the frequency offset caused by the Doppler effect and the oscillator instability. This paper proposes a non-coherent sequence detecti...The satellite-based automatic identification system (AIS) receiver has to encounter the frequency offset caused by the Doppler effect and the oscillator instability. This paper proposes a non-coherent sequence detection scheme for the satellite-based AIS signal transmitted over the white Gaussian noise channel. Based on the maximum likelihood estimation and a Viterbi decoder, the proposed scheme is capable of tolerating a frequency offset up to 5% of the symbol rate. The complexity of the proposed scheme is reduced by the state-complexity reduction, which is based on per-survivor processing. Simulation results prove that the proposed non-coherent sequence detection scheme has high robustness to frequency offset compared to the relative scheme when messages collision exists.展开更多
A nucleic acid sequence-based amplification（NASBA）assay was established for the detection of Macrobrachium rosenbergii Nodavirus（MrNV）.The specific primers were designed according to the high conserved region of R...A nucleic acid sequence-based amplification（NASBA）assay was established for the detection of Macrobrachium rosenbergii Nodavirus（MrNV）.The specific primers were designed according to the high conserved region of RNA2 sequence of MrNV.The 224 bp specific amplification product was obtained in positive sample determined with 3%agarose gel electrophoresis,while no product was generated from shrimp infected with other viruses including DNA viruses（IHHNV,WSSV）and RNA viruses（TSV,IMNV,YHV）.The detecting limit of the assay was 8pg nucleic acid,which is more sensitive than that of PCR method.展开更多
In the present paper, we discuss some properties of piecewise linear spectral sequences introduced by Liu and Xu. We have a study on the pointwise and almost everywhere convergence of its corresponding series. Also, i...In the present paper, we discuss some properties of piecewise linear spectral sequences introduced by Liu and Xu. We have a study on the pointwise and almost everywhere convergence of its corresponding series. Also, it is shown that the set G constructed from piecewise linear spectral sequences are bases, but not unconditional bases, for LP（0, 1） where 1 〈 p 〈 ∞, p ≠2.展开更多
Base-level is a kind of surface which controls sedimentation and erosion. So, it can be concluded that it is base-level change that controls the formation and internal structure of a sequence. A single cycle of base-l...Base-level is a kind of surface which controls sedimentation and erosion. So, it can be concluded that it is base-level change that controls the formation and internal structure of a sequence. A single cycle of base-level change can generate four sets of different stacking patterns. They are two sets of aggradation, one progradation and one retrogradation, which affects the features of the internal structure of a sequence. Lishu fault subsidence of Songliao basin is a typical half-graben lacustrine basin. Comprehensive base-level change analysis indicates that six base-level cycles and their related six sequences can be recognized between T 4 and T 5 seismic reflection surface. The contemporaneous fault is the main controlling factor of the fault lacustrine basin. There are obvious differences exist in the composition of sedimentary systems and all systems tracts between its steep slope (the side that basin control fault existed) and flat slope. Except highstand systems tract is composed of fan delta-lacustrine system, lowstand systems tract, transgressive systems tract and regressive systems tract are all made up of fan delta-underwater fan-lacustrine sedimentary systems in the side of steep slope.展开更多
In ports,inbound and outbound ships usually need tugboats to provide berthing and unberthing services.The decision-making problem on tugboat scheduling is important because it involves not only ships’turnaround time ...In ports,inbound and outbound ships usually need tugboats to provide berthing and unberthing services.The decision-making problem on tugboat scheduling is important because it involves not only ships’turnaround time at port but also tugboat operation costs.Encouraged by the problem faced by the tugboat operator,we formulate a mixed-integer programming model for tugboat scheduling problem with several practical constraints considered,such as dynamic arrival and departure of ships,qualification of tugboats,synchronization,and a flexible returning way to base to minimize the tugboat operation costs generated within the planning period.The model is inspired by genetic algorithm framework with three-dimensional coding.Effectiveness of our model and proposed solution method are testified and validated through experiments and computational results.This research helps to provide a scientific scheduling method and some insights for managers.展开更多
To understand the gene-based biological processes in-depth,the single-molecule real-time sequencing has drawn increasing attention with promoted by the Human Genome Project.Herein,a set of newly designed canonical flu...To understand the gene-based biological processes in-depth,the single-molecule real-time sequencing has drawn increasing attention with promoted by the Human Genome Project.Herein,a set of newly designed canonical fluorescent bases(A_(y),tC,G_(b),T_(p))are proposed for four-color DNA sequencing.These quasi-intrinsic probes are derived from the fluorophore replacement and ring expansion on natural bases,which still keep the pyrimidine or purine underlying skeleton and Watson–Crick hydrogen bonding face to allow minimal perturbation to the native DNA duplex.More importantly,these nucleobase analogues possess red-shifted absorption and efficient photoluminescence due to the enhancedπ-conjugation in character.Meanwhile,the four analogues could generate distinct emission wavelength(Δλ~50 nm)for real-time sequencing.To assess the biological employment of the proposed biosensors,the effects of base pairing and linking deoxyribose are also considered.展开更多
BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cau...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cause molecular variations in ESCC patients via genomic sequencing 299 samples.METHODS Here,we sequenced samples from 299 ESCC patients collected from Henan Key Laboratory for Esophageal Cancer Research and National Key Laboratory of Metabolic Dysregulation and Esophageal Cancer Prevention and Treatment,The First Affiliated Hospital of Zhengzhou University,including Han and Kazakh ethnic groups,and performed a genomic comparative analysis of these two ethnic cohorts.RESULTS ESCC patients of Kazakh ethnicity present with a later age of onset compared to Han.Kazakh patients exhibit a slightly higher tumor mutation burden compared to their Han counterparts.Three genes GIGYF1,CACNA1D,and ACOT11 exhibited mutation frequencies threefold higher in Kazakh patients than in Han.This enrichment may be associated with Kazakhs’adaptation to cold climates and consumption of high-calorie diets.Among Han patients,the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptide(APOBEC)-associated single base substitutions(SBS)13 mutational signature is more prevalent,whereas SBS6,indicative of DNA mismatch repair deficiency,is more common in Kazakh patients.Additionally,Han Chinese patients with APOBEC-enriched tumors exhibit a significantly higher mutation load than those without.Moreover,patients lacking the APOBEC signature demonstrate superior survival probability compared to the APOBEC-enriched group.CONCLUSION Living environment and diet are major factors in the development of ESCC.Genomic difference may provide guidance for the formulation of clinical treatment plans for ESCC from different ethnics regions.展开更多
Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍...Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍型相近或者相同的数据时,难以进行谱系的精细化区分。基于二代测序技术解析Y-STR的序列信息为解决上述困境带来了新的路径,并已成功应用于实际案例。然而,当前Y-STR序列特征的基础数据积累显著不足,序列多态性的研究也较为零散,缺乏对Y-STR序列变异的系统性总结,在一定程度上限制了Y-STR序列多态性在法庭科学的深度应用。本文基于文献公开数据,归纳在法庭科学中应用较广泛的41个Y-STR基因座重复区及侧翼区的序列特征,梳理Y-STR基因座重复区和侧翼区内可定义单倍群的单核苷酸多态性,并展望序列多态性可以拓展应用的场景,以期为利用Y-STR序列多态性精细化区分男性谱系、溯源父系生物地理祖先等研究提供参考。展开更多
文摘A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the pulses efficiently.Other PDW information such as rise time,carrier frequency,pulse width,modulation on pulse,fall time and direction of arrival are not required.To identify the valid PRIs in a set of interleaved pulses,an innovative modification of the DBSCAN algorithm is introduced which is accurate and easy to implement.The proposed method determines valid PRIs more accurately and neglects the spurious ones more efficiently as compared to the classical histogram based algorithms such as SDIF.Furthermore,without specifying any input parameter,the proposed method can deinterleave radar pulses while up to 30%jitter is present in the associated PRI.The accuracy and efficiency of the proposed method are verified by computer simulations and real data results.Experimental simulations are based on different real and operational scenarios where the presence of missing and spurious pulses are also considered.So,the simulation results can be of practical significance.
文摘The nucleotide (base) sequence of the genome might reflect biological information beyond the coding sequences. The appearance frequencies of successive base sequences (key sequences) were calculated for entire genomes. Based on the appearance frequency of the key sequences of the genome, any DNA sequences on the genome could be expressed as a sequence spectrum with the adjoining base sequences, which could be used to study the corresponding biological phenomena. In this paper, we used 64 successive three- base sequences (triplets) as the key sequences, and determined and compared the spectra of specific genes to the chromosome, or specific genes to tRNA genes in Saccharomyces cerevisiae, Schizosaccharomyces pombe and Escherichia coli. Based on these analyses, a gene and its corresponding position on the chromosome showed highly similar spectra with the same fold enlargement (approximately 400-fold) in the S. cerevisiae, S. pombe and E. coli genomes. In addition, the homologous structure of genes that encode proteins was also observed with appropriate tRNA gene(s) in the genome. This analytical method might faithfully reflect the encoded biological information, that is, the conservation of the base sequences was to make sense the conservation of the translated amino acids sequence in the coding region, and might be universally applicable to other genomes, even those that consisted of multiple chromosomes.
文摘A novel diagrammtic method is proposed to show the angular distribution of bases of human protein sequences. Using this method, the distribution sphere[1-4] is divided into four regions with same volume. The picture is clearer and more intuitive than that in [1] .A rule on the angular distribution of the representative points of bases of protein sequences is given. Besides, in 300 representative pointS of human protein sequence samples we find that there are three (not only one) points outside the sphere.
基金supported partially by the National Natural Science Foundation of China (10971105 and 10990012)the Natural Science Foundation of Tianjin (09JCYBJC01000)
文摘In this paper, we study the invertibility of sequences consisting of finitely many bounded linear operators from a Hilbert space to others. We show that a sequence of operators is left invertible if and only if it is a g-frame. Therefore, our result connects the invertibility of operator sequences with frame theory.
文摘The satellite-based automatic identification system (AIS) receiver has to encounter the frequency offset caused by the Doppler effect and the oscillator instability. This paper proposes a non-coherent sequence detection scheme for the satellite-based AIS signal transmitted over the white Gaussian noise channel. Based on the maximum likelihood estimation and a Viterbi decoder, the proposed scheme is capable of tolerating a frequency offset up to 5% of the symbol rate. The complexity of the proposed scheme is reduced by the state-complexity reduction, which is based on per-survivor processing. Simulation results prove that the proposed non-coherent sequence detection scheme has high robustness to frequency offset compared to the relative scheme when messages collision exists.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201103034)Huzhou Science and Technology Project(2012GN08,2011ZD2005)Science and Technology Innovation Team Project of Freshwater Aquaculture of Zhejiang Province(2012R10026-11)
文摘A nucleic acid sequence-based amplification（NASBA）assay was established for the detection of Macrobrachium rosenbergii Nodavirus（MrNV）.The specific primers were designed according to the high conserved region of RNA2 sequence of MrNV.The 224 bp specific amplification product was obtained in positive sample determined with 3%agarose gel electrophoresis,while no product was generated from shrimp infected with other viruses including DNA viruses（IHHNV,WSSV）and RNA viruses（TSV,IMNV,YHV）.The detecting limit of the assay was 8pg nucleic acid,which is more sensitive than that of PCR method.
基金Supported by Prof.Y.Xu under his grant in program of"One Hundred Distingulshed Chi-nese Scientists"of the Chinese Academy of Sciences,the NNSF(10371122)of China and Poetgraduate Innovation Fund of Chinese Academy of Sciences.
文摘In the present paper, we discuss some properties of piecewise linear spectral sequences introduced by Liu and Xu. We have a study on the pointwise and almost everywhere convergence of its corresponding series. Also, it is shown that the set G constructed from piecewise linear spectral sequences are bases, but not unconditional bases, for LP（0, 1） where 1 〈 p 〈 ∞, p ≠2.
文摘Base-level is a kind of surface which controls sedimentation and erosion. So, it can be concluded that it is base-level change that controls the formation and internal structure of a sequence. A single cycle of base-level change can generate four sets of different stacking patterns. They are two sets of aggradation, one progradation and one retrogradation, which affects the features of the internal structure of a sequence. Lishu fault subsidence of Songliao basin is a typical half-graben lacustrine basin. Comprehensive base-level change analysis indicates that six base-level cycles and their related six sequences can be recognized between T 4 and T 5 seismic reflection surface. The contemporaneous fault is the main controlling factor of the fault lacustrine basin. There are obvious differences exist in the composition of sedimentary systems and all systems tracts between its steep slope (the side that basin control fault existed) and flat slope. Except highstand systems tract is composed of fan delta-lacustrine system, lowstand systems tract, transgressive systems tract and regressive systems tract are all made up of fan delta-underwater fan-lacustrine sedimentary systems in the side of steep slope.
基金the National Social Science Foundation of China(No.22BGJ034)。
文摘In ports,inbound and outbound ships usually need tugboats to provide berthing and unberthing services.The decision-making problem on tugboat scheduling is important because it involves not only ships’turnaround time at port but also tugboat operation costs.Encouraged by the problem faced by the tugboat operator,we formulate a mixed-integer programming model for tugboat scheduling problem with several practical constraints considered,such as dynamic arrival and departure of ships,qualification of tugboats,synchronization,and a flexible returning way to base to minimize the tugboat operation costs generated within the planning period.The model is inspired by genetic algorithm framework with three-dimensional coding.Effectiveness of our model and proposed solution method are testified and validated through experiments and computational results.This research helps to provide a scientific scheduling method and some insights for managers.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11804195,11847224,11674198,and 12274265)the Natural Science Foundation of Shandong Province,China(Grant Nos.ZR2018BA034 and ZR2022MA006)。
文摘To understand the gene-based biological processes in-depth,the single-molecule real-time sequencing has drawn increasing attention with promoted by the Human Genome Project.Herein,a set of newly designed canonical fluorescent bases(A_(y),tC,G_(b),T_(p))are proposed for four-color DNA sequencing.These quasi-intrinsic probes are derived from the fluorophore replacement and ring expansion on natural bases,which still keep the pyrimidine or purine underlying skeleton and Watson–Crick hydrogen bonding face to allow minimal perturbation to the native DNA duplex.More importantly,these nucleobase analogues possess red-shifted absorption and efficient photoluminescence due to the enhancedπ-conjugation in character.Meanwhile,the four analogues could generate distinct emission wavelength(Δλ~50 nm)for real-time sequencing.To assess the biological employment of the proposed biosensors,the effects of base pairing and linking deoxyribose are also considered.
基金Supported by the National Natural Science Foundation of China,No.U1301227National Major Science and Technology Projects,No.2024ZD05209060Henan Province Jointly Built Science and Technology Key Projects,No.LHGJ20210337.
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cause molecular variations in ESCC patients via genomic sequencing 299 samples.METHODS Here,we sequenced samples from 299 ESCC patients collected from Henan Key Laboratory for Esophageal Cancer Research and National Key Laboratory of Metabolic Dysregulation and Esophageal Cancer Prevention and Treatment,The First Affiliated Hospital of Zhengzhou University,including Han and Kazakh ethnic groups,and performed a genomic comparative analysis of these two ethnic cohorts.RESULTS ESCC patients of Kazakh ethnicity present with a later age of onset compared to Han.Kazakh patients exhibit a slightly higher tumor mutation burden compared to their Han counterparts.Three genes GIGYF1,CACNA1D,and ACOT11 exhibited mutation frequencies threefold higher in Kazakh patients than in Han.This enrichment may be associated with Kazakhs’adaptation to cold climates and consumption of high-calorie diets.Among Han patients,the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptide(APOBEC)-associated single base substitutions(SBS)13 mutational signature is more prevalent,whereas SBS6,indicative of DNA mismatch repair deficiency,is more common in Kazakh patients.Additionally,Han Chinese patients with APOBEC-enriched tumors exhibit a significantly higher mutation load than those without.Moreover,patients lacking the APOBEC signature demonstrate superior survival probability compared to the APOBEC-enriched group.CONCLUSION Living environment and diet are major factors in the development of ESCC.Genomic difference may provide guidance for the formulation of clinical treatment plans for ESCC from different ethnics regions.
文摘Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍型相近或者相同的数据时,难以进行谱系的精细化区分。基于二代测序技术解析Y-STR的序列信息为解决上述困境带来了新的路径,并已成功应用于实际案例。然而,当前Y-STR序列特征的基础数据积累显著不足,序列多态性的研究也较为零散,缺乏对Y-STR序列变异的系统性总结,在一定程度上限制了Y-STR序列多态性在法庭科学的深度应用。本文基于文献公开数据,归纳在法庭科学中应用较广泛的41个Y-STR基因座重复区及侧翼区的序列特征,梳理Y-STR基因座重复区和侧翼区内可定义单倍群的单核苷酸多态性,并展望序列多态性可以拓展应用的场景,以期为利用Y-STR序列多态性精细化区分男性谱系、溯源父系生物地理祖先等研究提供参考。
