We have cloned the E6 gene of human papillomavirus type 18 into anexpression plasmid pBD2.One of the recombinant plasmids (named pDV11) wasidentified by DNA analysis and protein product analysis.It could express a new...We have cloned the E6 gene of human papillomavirus type 18 into anexpression plasmid pBD2.One of the recombinant plasmids (named pDV11) wasidentified by DNA analysis and protein product analysis.It could express a newprotein whose molecular weight correspods well with the expected one.Afterpurification,the expressed protein showed a positive result in countercurrentimmuno-electrophoresis with anti-β-gal serum and was proved to be the expectedβ-gal/E6 fusion protein.The physical map of pDV11 was also prepared.展开更多
In this article,the basic concept,constitute and brief development history of Nuclear Explosions Fusion Power Plant is introduced.A series of technique is put forward to solve the implement safety of nuclear explosion...In this article,the basic concept,constitute and brief development history of Nuclear Explosions Fusion Power Plant is introduced.A series of technique is put forward to solve the implement safety of nuclear explosion;the designs of nuclear devicein deuterium-type and the reclamation of nuclear fuel are put forward.The technique possibility of power station is analyzed,and the prospect of all kinds of nuclear energy project to provide energy of the mankind future are compared.展开更多
BACKGROUND Colorectal cancer(CRC)is the second leading cause of cancer-related death,lar-gely due to limited treatment options in advanced stages.Genomic alterations in advanced CRC(aCRC)are complex and not fully char...BACKGROUND Colorectal cancer(CRC)is the second leading cause of cancer-related death,lar-gely due to limited treatment options in advanced stages.Genomic alterations in advanced CRC(aCRC)are complex and not fully characterized,with only 30%of patients benefiting from targeted therapies.AIM To investigate the molecular heterogeneity of primary aCRC in order to identify clinically relevant genomic alterations.METHODS We conducted a retrospective molecular analysis of 73 consecutive patients with histologically confirmed primary aCRC(stage pT4a-b).All molecular findings were correlated with available clinicopathological data.In addition,we performed RESULTS Genetic abnormalities identified in primary tumors were most frequently mutations in tumor protein p53(58%of cases),Kirsten rat sarcoma viral oncogene homolog(52%),phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(25%),B-Raf kinase(11%)and fibroblast growth factor receptor 3(8%),as well as R-spondin 3(RSPO3)fusions(8%).Alterations in the tumor protein p53 and neuroblastoma RAS viral oncogene homolog genes were predominantly observed in tumors from the left colon,whereas B-Raf kinase mutations and RSPO3 fusions were more frequently detected in the right or transverse colon.We also show a strong association between the presence of RSPO3 rearrangements and patients with small tumors,normal carcinoembryonic antigen levels,and microsatellite stable tumors.Furthermore,aCRC patients with protein tyrosine phosphatase receptor type k::RSPO3 fusions exhibited a higher mortality rate.Elevated RSPO3 gene expression levels were also significantly correlated with poorer OS across two large,independent CRC cohorts.CONCLUSION This study identifies a relatively high incidence of RSPO3 rearrangements in aCRC and a strong association with clinical features.Furthermore,we find that RSPO3 fusions are associated with poorer OS.展开更多
基金The project was supported by National Natural Science Foundation of China
文摘We have cloned the E6 gene of human papillomavirus type 18 into anexpression plasmid pBD2.One of the recombinant plasmids (named pDV11) wasidentified by DNA analysis and protein product analysis.It could express a newprotein whose molecular weight correspods well with the expected one.Afterpurification,the expressed protein showed a positive result in countercurrentimmuno-electrophoresis with anti-β-gal serum and was proved to be the expectedβ-gal/E6 fusion protein.The physical map of pDV11 was also prepared.
文摘In this article,the basic concept,constitute and brief development history of Nuclear Explosions Fusion Power Plant is introduced.A series of technique is put forward to solve the implement safety of nuclear explosion;the designs of nuclear devicein deuterium-type and the reclamation of nuclear fuel are put forward.The technique possibility of power station is analyzed,and the prospect of all kinds of nuclear energy project to provide energy of the mankind future are compared.
文摘BACKGROUND Colorectal cancer(CRC)is the second leading cause of cancer-related death,lar-gely due to limited treatment options in advanced stages.Genomic alterations in advanced CRC(aCRC)are complex and not fully characterized,with only 30%of patients benefiting from targeted therapies.AIM To investigate the molecular heterogeneity of primary aCRC in order to identify clinically relevant genomic alterations.METHODS We conducted a retrospective molecular analysis of 73 consecutive patients with histologically confirmed primary aCRC(stage pT4a-b).All molecular findings were correlated with available clinicopathological data.In addition,we performed RESULTS Genetic abnormalities identified in primary tumors were most frequently mutations in tumor protein p53(58%of cases),Kirsten rat sarcoma viral oncogene homolog(52%),phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(25%),B-Raf kinase(11%)and fibroblast growth factor receptor 3(8%),as well as R-spondin 3(RSPO3)fusions(8%).Alterations in the tumor protein p53 and neuroblastoma RAS viral oncogene homolog genes were predominantly observed in tumors from the left colon,whereas B-Raf kinase mutations and RSPO3 fusions were more frequently detected in the right or transverse colon.We also show a strong association between the presence of RSPO3 rearrangements and patients with small tumors,normal carcinoembryonic antigen levels,and microsatellite stable tumors.Furthermore,aCRC patients with protein tyrosine phosphatase receptor type k::RSPO3 fusions exhibited a higher mortality rate.Elevated RSPO3 gene expression levels were also significantly correlated with poorer OS across two large,independent CRC cohorts.CONCLUSION This study identifies a relatively high incidence of RSPO3 rearrangements in aCRC and a strong association with clinical features.Furthermore,we find that RSPO3 fusions are associated with poorer OS.
