Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn...Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn SD rats and the sagittal suture from the mice, were cultured with a serum-free medium and treated with and without insulin-like growth factor 1. The osteoblast phetotypes (osteocalcin, alkaline, osteoponcin and type-1 collagen) were measured with the RT -PCR and ELISA, and the explanted sagittal sutures were then evaluated under light microscopy. Results The cells, treated with the insulin-like growth factor 1, significantly produced more osteocalcin, alkaline, osteoponcin and type-1 collagen than those without insulin-like growth factor 1. The fusion of the sagittal suture explants will delay till to 30 days when it was not treated with IGF1. However, in the group with IGF1 the fusion was observed to start in 8 days, and a small amount of the sagittal suture fusion was found at the 20th展开更多
Objective Loss-of-function mutation of p53,a tumor suppressor gene,is an important mechanism for the development of human cancers. In this study we tried to transfect p53N15-based fusion peptide into H1299,a lung canc...Objective Loss-of-function mutation of p53,a tumor suppressor gene,is an important mechanism for the development of human cancers. In this study we tried to transfect p53N15-based fusion peptide into H1299,a lung cancer cell line,and evaluate the anti-tumor effects of the fusion peptide. Methods Adeno-associated virus (AAV) vectors were used for transfecting p53N15 fusion peptide into p53-null lung adenocarcinoma H1299 cells.展开更多
A key step is to extract valid information region in the fusion of multi-voltage X-ray image sequence for complicated components. To improve the self-adaption of extraction, a method is presented in this paper. In thi...A key step is to extract valid information region in the fusion of multi-voltage X-ray image sequence for complicated components. To improve the self-adaption of extraction, a method is presented in this paper. In this paper, the valid informa-tion region is selected by the grey level interval, which is computed by the optimization of image quality evaluation model. The model is based on the histogram equalization and the grey level interval. Then, every valid region of images at different voltages is extracted and they are fused according their grey level transformation function. The fusion image contains completed struc-ture information of the component. The fusion experiment of a cylinder head shows the effectiveness of the presented method.展开更多
To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant euk...To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE elec- trophoresis were used to determine the stable transfection and expression of recombinant protein. Each mouse was inoculated with plasmids intramuscularly （i.m.） three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies （NAb） were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1：20-1：80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine.展开更多
Quantum effects play an enhancement role in p-p chain reactions occurring within stars. Such an enhancement is quantified by a wave penetration factor that is proportional to the density of the participating fuel part...Quantum effects play an enhancement role in p-p chain reactions occurring within stars. Such an enhancement is quantified by a wave penetration factor that is proportional to the density of the participating fuel particles. This leads to an innovative theory for dense plasma, and its result shows good agreement with independent data derived from the solar energy output. An analysis of the first Z-pinch machine in mankind＇s history exhibiting neutron emission leads to a derived deuterium plasma beam density greater than that of water, with plasma velocities exceeding 10000 km/s. Fusion power could be achieved by the intersection of four such pinched plasma beams with powerful head-on collisions in their common focal region due to the beam and target enhanced reaction.展开更多
Using a compact heap of Fullerene nano particle moduli of a nano matrix device we propose that by maximizing the Casimir forces between these particles as a desirable effect, we can achieve a gradual rather than a sud...Using a compact heap of Fullerene nano particle moduli of a nano matrix device we propose that by maximizing the Casimir forces between these particles as a desirable effect, we can achieve a gradual rather than a sudden implosion pressure. This we expect will result in a mini holographic universe from which energy can be extracted in a way constituting a nano energy reactor functioning effectively on a hybrid principle somewhere between a Casimir effect and a cold fusion process.展开更多
Up to now, no a real full-cover liquid metal (LM) free surface flow have been successfully used in magnetic fusion devices as MHD instability and unavoidable rivulet flow. Recently, after we carried out a guidable fre...Up to now, no a real full-cover liquid metal (LM) free surface flow have been successfully used in magnetic fusion devices as MHD instability and unavoidable rivulet flow. Recently, after we carried out a guidable free curve-surface flow on theoretically and experimentally, seeking for other way to get a full-cover free surface flow is also in implementing. The superficial layer MHD effect in free surface flow is experimentally observed. After compared and analyzed the characteristic parameters of the free surface flow, the conditions of full-cover free surface flow are found. Meanwhile, the new two parameters of surface cover ratio and rivulet flow index are introduced to characterize the flowing characteristic of the full-cover free surface flow under magnetic field. According to the analysis rule, for different liquid metal, there are the different unique conditions to meet full-cover free surface flow under magnetic field. This may be a way to solve free surface flow major MHD key issue for LM PFCs.展开更多
文摘Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn SD rats and the sagittal suture from the mice, were cultured with a serum-free medium and treated with and without insulin-like growth factor 1. The osteoblast phetotypes (osteocalcin, alkaline, osteoponcin and type-1 collagen) were measured with the RT -PCR and ELISA, and the explanted sagittal sutures were then evaluated under light microscopy. Results The cells, treated with the insulin-like growth factor 1, significantly produced more osteocalcin, alkaline, osteoponcin and type-1 collagen than those without insulin-like growth factor 1. The fusion of the sagittal suture explants will delay till to 30 days when it was not treated with IGF1. However, in the group with IGF1 the fusion was observed to start in 8 days, and a small amount of the sagittal suture fusion was found at the 20th
文摘Objective Loss-of-function mutation of p53,a tumor suppressor gene,is an important mechanism for the development of human cancers. In this study we tried to transfect p53N15-based fusion peptide into H1299,a lung cancer cell line,and evaluate the anti-tumor effects of the fusion peptide. Methods Adeno-associated virus (AAV) vectors were used for transfecting p53N15 fusion peptide into p53-null lung adenocarcinoma H1299 cells.
基金National Natural Science Foundation of China(No.61227003,No.61301259,No.61471325and No.61571404)Natural Science Foundation of Shanxi Province(No.2015021099)
文摘A key step is to extract valid information region in the fusion of multi-voltage X-ray image sequence for complicated components. To improve the self-adaption of extraction, a method is presented in this paper. In this paper, the valid informa-tion region is selected by the grey level interval, which is computed by the optimization of image quality evaluation model. The model is based on the histogram equalization and the grey level interval. Then, every valid region of images at different voltages is extracted and they are fused according their grey level transformation function. The fusion image contains completed struc-ture information of the component. The fusion experiment of a cylinder head shows the effectiveness of the presented method.
基金This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30170819)
文摘To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE elec- trophoresis were used to determine the stable transfection and expression of recombinant protein. Each mouse was inoculated with plasmids intramuscularly （i.m.） three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies （NAb） were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1：20-1：80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine.
基金supported by the Fund for the Construction of Graduate Degree of China(No.2014XWD-S0805)
文摘Quantum effects play an enhancement role in p-p chain reactions occurring within stars. Such an enhancement is quantified by a wave penetration factor that is proportional to the density of the participating fuel particles. This leads to an innovative theory for dense plasma, and its result shows good agreement with independent data derived from the solar energy output. An analysis of the first Z-pinch machine in mankind＇s history exhibiting neutron emission leads to a derived deuterium plasma beam density greater than that of water, with plasma velocities exceeding 10000 km/s. Fusion power could be achieved by the intersection of four such pinched plasma beams with powerful head-on collisions in their common focal region due to the beam and target enhanced reaction.
文摘Using a compact heap of Fullerene nano particle moduli of a nano matrix device we propose that by maximizing the Casimir forces between these particles as a desirable effect, we can achieve a gradual rather than a sudden implosion pressure. This we expect will result in a mini holographic universe from which energy can be extracted in a way constituting a nano energy reactor functioning effectively on a hybrid principle somewhere between a Casimir effect and a cold fusion process.
文摘Up to now, no a real full-cover liquid metal (LM) free surface flow have been successfully used in magnetic fusion devices as MHD instability and unavoidable rivulet flow. Recently, after we carried out a guidable free curve-surface flow on theoretically and experimentally, seeking for other way to get a full-cover free surface flow is also in implementing. The superficial layer MHD effect in free surface flow is experimentally observed. After compared and analyzed the characteristic parameters of the free surface flow, the conditions of full-cover free surface flow are found. Meanwhile, the new two parameters of surface cover ratio and rivulet flow index are introduced to characterize the flowing characteristic of the full-cover free surface flow under magnetic field. According to the analysis rule, for different liquid metal, there are the different unique conditions to meet full-cover free surface flow under magnetic field. This may be a way to solve free surface flow major MHD key issue for LM PFCs.
