Tsetse(Glossina spp.)are vectors of African trypanosomes that cause devastating human and animal African trypanosomiases.While much of the research to better understand tsetse genetics and physiology relies on colony-...Tsetse(Glossina spp.)are vectors of African trypanosomes that cause devastating human and animal African trypanosomiases.While much of the research to better understand tsetse genetics and physiology relies on colony-reared flies,these flies may not represent the genetic diversity found in natural wild populations due to their long-term captivity.To enhance the translation of colony research into field applications,we utilized Nanopore sequencing to assemble genomes for a wild-caught female Glossina fuscipes fuscipes(Gff)from northwestern Uganda and for a female Gff from a laboratory line originally sourced from the Central African Republic in 1986.The new assemblies,from the wild-caught Gff(405.98 Mb,N50:56.86 Mb)and the laboratory-derived Gff(398.22 Mb,N50:47.811 Mb),demonstrate near-chromosomal level contiguity,high BUSCO scores(>99.5%),high QV scores(>37),and over 12345 genes.Alignments between both new genomes reveal conserved synteny with only minor structural variants in the X,1L,1R,2L,and 2R tsetse chromosomes.While most orthologs(10730)were shared between both new genomes,we identified 381 unique orthologs and a small number of highly diverged shared single-copy homologs(3.84%).These gene-set differences could represent population-level variation due to the distinct geographic origin of these flies or adaptation to colony conditions.Our new high-quality genomes,with improvements in contiguity and completeness compared to the current NCBI RefSeq Gff genome,lay the foundation for advanced tsetse research,enabling robust lab-to-field translational applications to deepen our understanding of vector biology and disease transmission dynamics.展开更多
基金support provided by the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture,Insect Pest Control Subprograms under the CRP D4201supported with funding from Ambrose Monell Foundation(to SA),and National Institutes of Health(R01AI068932 and R01AI139525 to SA)National Institutes of Health(R21AI163969 to SA and BW).
文摘Tsetse(Glossina spp.)are vectors of African trypanosomes that cause devastating human and animal African trypanosomiases.While much of the research to better understand tsetse genetics and physiology relies on colony-reared flies,these flies may not represent the genetic diversity found in natural wild populations due to their long-term captivity.To enhance the translation of colony research into field applications,we utilized Nanopore sequencing to assemble genomes for a wild-caught female Glossina fuscipes fuscipes(Gff)from northwestern Uganda and for a female Gff from a laboratory line originally sourced from the Central African Republic in 1986.The new assemblies,from the wild-caught Gff(405.98 Mb,N50:56.86 Mb)and the laboratory-derived Gff(398.22 Mb,N50:47.811 Mb),demonstrate near-chromosomal level contiguity,high BUSCO scores(>99.5%),high QV scores(>37),and over 12345 genes.Alignments between both new genomes reveal conserved synteny with only minor structural variants in the X,1L,1R,2L,and 2R tsetse chromosomes.While most orthologs(10730)were shared between both new genomes,we identified 381 unique orthologs and a small number of highly diverged shared single-copy homologs(3.84%).These gene-set differences could represent population-level variation due to the distinct geographic origin of these flies or adaptation to colony conditions.Our new high-quality genomes,with improvements in contiguity and completeness compared to the current NCBI RefSeq Gff genome,lay the foundation for advanced tsetse research,enabling robust lab-to-field translational applications to deepen our understanding of vector biology and disease transmission dynamics.
