To solve the problems of abnormal larger, abnormal lower or even negative of target yield and fertilizing amount recommended by part of non-typical fertilizer effect equations using agricultural experiments and statis...To solve the problems of abnormal larger, abnormal lower or even negative of target yield and fertilizing amount recommended by part of non-typical fertilizer effect equations using agricultural experiments and statistical analysis software,Yangzhou analyzer（2.2）, regression analysis of Excel, which objected to local actual production, the study adopted the principle and method of basic knowledge and the frequency of using probability theory, and carried out statistical analysis on the rape field fertilizer experiment data by frequency analysis method, the rape yield after optimizing fertilizing amount was 1 732.4 kg/hm^2, the ranges of N, P and K optimal combinations were： N=210.36-149.64 kg/hm^2,P2O5=81.89-58.11 kg/hm^2, K2O=81.89-58.11 kg/hm^2,which was consistent with local actual production. This study was based on frequency analysis, using weighted average method to determine the production combinations of different yield objectives, hereinto, the combinations with high yield, high frequency of occurrence（dependable crop） and fertilizer-saving were viewed as the optimizing production measures, and they had the merits of increasing fertilization decision-making information, reducing or avoiding the risk of small probability event. The results of this study can solve the problem of abnormal values fertilizing amount and target yield recommended by non-typical fertilizer effect function, which did not accord with local actual production, caused by Yangzhou analyzer（2.2）, regression analysis of Excel, and DPS statistical analysis software. For the fertilizer effect function equation established by regression analysis which did not reach significance level using variance analysis, whether the method can be adapted to for carrying out fertilization decision-making, recommending optimization combinations of N, P and K fertilizers and yield under optimized fertilizing amount should be further researched in future working practice.展开更多
Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental ...Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertilizer with organic amendments incorporated（NPKM, NPKS） could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis（PCA） based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis（RDA） indicated that soil organic carbon（SOC） availability, especially soil microbial biomass carbon（Cmic） and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China＇s soil resource.展开更多
In order to increase mulberry leaf yield and production efficiency in hilly areas of Sichuan Province through scientific fertilization,＂3414＂,a quadratic regression design for modern fertilization,was employed to si...In order to increase mulberry leaf yield and production efficiency in hilly areas of Sichuan Province through scientific fertilization,＂3414＂,a quadratic regression design for modern fertilization,was employed to simulate the regression relationship between mulberry leaf yield and major fertilizer elements including nitrogen（N）,phosphor（P） and potassium（K） among which mulberry leaf yield was the objective function and N,P and K were the regulatory factors to fertilization level.As a result,7 types of ternary,binary and monadic quadratic fertilizer effect functions of N,P and K fertilizer elements were established.The weight of fertilizer factors in the optimal mulberry leaf yield obtained by various types of functions was in decreasing order of KPKPNPKNKNNP,and the weight of fertilizer factors in the ratio of leaf yield to input was in decreasing order of K〉PK〉NPK〉P〉NK〉NP〉N.To be specific,the ternary quadratic fertilizer effect function（Y=20 313＋0.25X1＋43.48 X2＋34.47X3-0.01X1^2-0.14X2^2-0.06X3^2＋0.05X1X2＋0.02X1X3-0.07X2X3,in which X1,X2 and X3 indicate the fertilizer factors N,P and K,respectively） was a model containing all factors,thereby being a recommended fertilization program for mulberry field.Single factor effect analysis indicated that K fertilizer had the highest influence on mulberry leaf yield and ratio of leaf yield to input.Therefore,among all the simulation functions,the monadic quadratic fertilizer effect function of K fertilizer factor（Y=25 002.27＋44.728X3-0.084X32） leads to the optimal leaf yield and maximum ratio of leaf yield to input.By using the optimal fertilization program with 600 kg/hm2 N,210 kg/hm2 P2O5 and 254.33 kg/hm2 K2O,the optimal economic leaf yield obtained is 30 945.00 kg/hm2,the cost of fertilizer input is 4 403.65 yuan/hm2,and the ratio of leaf yield to input is as high as 17.57.After verification by production practices,the recommended fertilization indicators in the optimal mulberry fertilization program can provide reference for the construction of high-yield mulberry field in hilly areas of Sichuan Province.展开更多
Paddy fields,as the largest anthropogenic wetlands on Earth,face a high risk of micronutrient loss through surface runoff and leaching due to their frequent irrigation-drainage cycles,as well as removal with crop harv...Paddy fields,as the largest anthropogenic wetlands on Earth,face a high risk of micronutrient loss through surface runoff and leaching due to their frequent irrigation-drainage cycles,as well as removal with crop harvest.While micronutrient’s losses largely impede biological nitrogen fixation(BNF)in soils,agricultural practices and mechanisms that retain micronutrients and thus increase BNF in paddy soils remain underexplored.Here we showed that the long-term(40 years)application of fertilizers increased the contents of microbial necromass in paddy soils by 20%-43%compared to the unfertilized control.In particular,long-term organic fertilizations increased poorly crystalline minerals through intensifying mineral weathering,which further contributed to the increased stable carbon burials in paddy soils.Synchrotron radiation based spectromicroscopy analysis provided direct evidence demonstrating a differential control of hydroxyl functional groups from mineral surfaces on C functional groups at the submicron scale in paddy soils.Notably,microbial necromass and short-range ordered minerals had a strong correlation with the content and bioavailability of micronutrients in paddy soils,indicating their key roles in the retention of micronutrients.Metagenomic sequencing analysis further indicated that the content and bioavailability of micronutrients were strongly correlated with the abundance of the key N-fixing genera(i.e.,Azospirillum and Bradyrhizobium).Unexpectedly,structural equation modeling(SEM)identified that microbial necromass exerted the strongest control on N-fixing genera,highlighting an underappreciated role of microbial necromass as a reservoir of micronutrients.Based on micronutrient's bioavailability and metagenomic sequencing,we conclude that micronutrients are the key factor for BNF in paddy soils,offering significant implications for managing BNF in paddy soils.展开更多
基金Supported by Fiscal Subsidy Project Fund of National Soil Testing and Formulated Fertilization(Yun Cai Nong[2009]2045)~~
文摘To solve the problems of abnormal larger, abnormal lower or even negative of target yield and fertilizing amount recommended by part of non-typical fertilizer effect equations using agricultural experiments and statistical analysis software,Yangzhou analyzer（2.2）, regression analysis of Excel, which objected to local actual production, the study adopted the principle and method of basic knowledge and the frequency of using probability theory, and carried out statistical analysis on the rape field fertilizer experiment data by frequency analysis method, the rape yield after optimizing fertilizing amount was 1 732.4 kg/hm^2, the ranges of N, P and K optimal combinations were： N=210.36-149.64 kg/hm^2,P2O5=81.89-58.11 kg/hm^2, K2O=81.89-58.11 kg/hm^2,which was consistent with local actual production. This study was based on frequency analysis, using weighted average method to determine the production combinations of different yield objectives, hereinto, the combinations with high yield, high frequency of occurrence（dependable crop） and fertilizer-saving were viewed as the optimizing production measures, and they had the merits of increasing fertilization decision-making information, reducing or avoiding the risk of small probability event. The results of this study can solve the problem of abnormal values fertilizing amount and target yield recommended by non-typical fertilizer effect function, which did not accord with local actual production, caused by Yangzhou analyzer（2.2）, regression analysis of Excel, and DPS statistical analysis software. For the fertilizer effect function equation established by regression analysis which did not reach significance level using variance analysis, whether the method can be adapted to for carrying out fertilization decision-making, recommending optimization combinations of N, P and K fertilizers and yield under optimized fertilizing amount should be further researched in future working practice.
基金funded by the National Natural Science Foundation of China(NSFC31301843)the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences(IARRP-202-5)
文摘Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertilizer with organic amendments incorporated（NPKM, NPKS） could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis（PCA） based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis（RDA） indicated that soil organic carbon（SOC） availability, especially soil microbial biomass carbon（Cmic） and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China＇s soil resource.
基金Supported by Excellent Paper Fund from Sichuan Academy of Agricultural Sciences(No.2011LWJJ-008)Breeding Genetic Engineering Project from Sichuan Provincial Department of Finance of the 12th Five-Year Plan(2011JYGC10-027-02)Special Fund for Construction of Modern Agricultural Industry Technology System(Sericulture)~~
文摘In order to increase mulberry leaf yield and production efficiency in hilly areas of Sichuan Province through scientific fertilization,＂3414＂,a quadratic regression design for modern fertilization,was employed to simulate the regression relationship between mulberry leaf yield and major fertilizer elements including nitrogen（N）,phosphor（P） and potassium（K） among which mulberry leaf yield was the objective function and N,P and K were the regulatory factors to fertilization level.As a result,7 types of ternary,binary and monadic quadratic fertilizer effect functions of N,P and K fertilizer elements were established.The weight of fertilizer factors in the optimal mulberry leaf yield obtained by various types of functions was in decreasing order of KPKPNPKNKNNP,and the weight of fertilizer factors in the ratio of leaf yield to input was in decreasing order of K〉PK〉NPK〉P〉NK〉NP〉N.To be specific,the ternary quadratic fertilizer effect function（Y=20 313＋0.25X1＋43.48 X2＋34.47X3-0.01X1^2-0.14X2^2-0.06X3^2＋0.05X1X2＋0.02X1X3-0.07X2X3,in which X1,X2 and X3 indicate the fertilizer factors N,P and K,respectively） was a model containing all factors,thereby being a recommended fertilization program for mulberry field.Single factor effect analysis indicated that K fertilizer had the highest influence on mulberry leaf yield and ratio of leaf yield to input.Therefore,among all the simulation functions,the monadic quadratic fertilizer effect function of K fertilizer factor（Y=25 002.27＋44.728X3-0.084X32） leads to the optimal leaf yield and maximum ratio of leaf yield to input.By using the optimal fertilization program with 600 kg/hm2 N,210 kg/hm2 P2O5 and 254.33 kg/hm2 K2O,the optimal economic leaf yield obtained is 30 945.00 kg/hm2,the cost of fertilizer input is 4 403.65 yuan/hm2,and the ratio of leaf yield to input is as high as 17.57.After verification by production practices,the recommended fertilization indicators in the optimal mulberry fertilization program can provide reference for the construction of high-yield mulberry field in hilly areas of Sichuan Province.
基金supported by the National Natural Science Foundation of China(U22A20608,42293264,and 41977271)Fujian Natural Science Foundation(2021J01479)+1 种基金Fujian Field Scientific Observation and Research Station of Minhou Farmland Ecosystem(MKJ[2018]No.17)“5511”Collaborative Innovation Project(XTCXGC2021009).
文摘Paddy fields,as the largest anthropogenic wetlands on Earth,face a high risk of micronutrient loss through surface runoff and leaching due to their frequent irrigation-drainage cycles,as well as removal with crop harvest.While micronutrient’s losses largely impede biological nitrogen fixation(BNF)in soils,agricultural practices and mechanisms that retain micronutrients and thus increase BNF in paddy soils remain underexplored.Here we showed that the long-term(40 years)application of fertilizers increased the contents of microbial necromass in paddy soils by 20%-43%compared to the unfertilized control.In particular,long-term organic fertilizations increased poorly crystalline minerals through intensifying mineral weathering,which further contributed to the increased stable carbon burials in paddy soils.Synchrotron radiation based spectromicroscopy analysis provided direct evidence demonstrating a differential control of hydroxyl functional groups from mineral surfaces on C functional groups at the submicron scale in paddy soils.Notably,microbial necromass and short-range ordered minerals had a strong correlation with the content and bioavailability of micronutrients in paddy soils,indicating their key roles in the retention of micronutrients.Metagenomic sequencing analysis further indicated that the content and bioavailability of micronutrients were strongly correlated with the abundance of the key N-fixing genera(i.e.,Azospirillum and Bradyrhizobium).Unexpectedly,structural equation modeling(SEM)identified that microbial necromass exerted the strongest control on N-fixing genera,highlighting an underappreciated role of microbial necromass as a reservoir of micronutrients.Based on micronutrient's bioavailability and metagenomic sequencing,we conclude that micronutrients are the key factor for BNF in paddy soils,offering significant implications for managing BNF in paddy soils.
