Pearl millet cultivar (Dempy) was supplemented with soybean flour to raise the protein content by 5%, 10% and 15%. Defatted soybean flour, pearl millet flour and their composite flour had varying functional properties...Pearl millet cultivar (Dempy) was supplemented with soybean flour to raise the protein content by 5%, 10% and 15%. Defatted soybean flour, pearl millet flour and their composite flour had varying functional properties. Water and fat absorption, bulk density, nitrogen solubility and dispersibility were higher for soybean flour compared to that of pearl millet flour. Therefore, such properties were improved significantly (P ≤ 0.05) for pearl millet with increasing the level of soybean supplementation. The emulsifying and foaming properties of pearl millet flour were poor but after supplementation with soybean they significantly (P ≤ 0.05) improved. Supplementation of pearl millet flour with soybean had no effect on least gelation concentration of millet. The implication of these results will be realized in designing protein-enriched products based on pearl millet flour, especially for pearl millet–growing regions in the under-developing countries.展开更多
An isolated liver function test is of little role in selection of liver disease because many harmful liver diseases may be correlated with normal levels of LFT’s. The outline of enzyme abnormalities in the perspectiv...An isolated liver function test is of little role in selection of liver disease because many harmful liver diseases may be correlated with normal levels of LFT’s. The outline of enzyme abnormalities in the perspective of patient’s commonly observed symptoms and laboratory data might be helpful in directing the subsequent diagnosis of liver diseases. Liver Function Tests (LFTs) are most generally used screening blood tests for assessment of different liver diseases and these tests provide a lot of evidence for disease processes whether for the purpose of investigation of supposed liver disease or help in observing the progress of disease action or simply by blood investigation. The evaluation of different liver enzymes simply gives diagnostic information on basic level whether patient’s principal disorder is actually hepatitis or cholestasis in source. However, it is necessary in various cases to evaluate LFTs with knowledge of liver functioning enzyme fractions. The objective of this study was to explore the effects of ascorbic acid supplementation on serum liver function tests in Hepatitis C patients. A total of 100 hepatitis C patients were selected randomly. 50 were given ascorbic acid supplementation for one month along with anti HCV treatment. The other 50 HCV patients took their normal anti HCV treatment without intake of ascorbic acid supplementation, and serum ascorbic acid level and liver function test parameters were observed before and after intake of ascorbic acid in both groups. The liver function parameters determined were aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), serum total bilirubin, direct bilirubin, indirect bilirubin and serum protein (total protein, albumin, globulin and A/G ratio). These parameters along with serum ascorbic acid were measured before and 30 days after vitamin C supplementation. Various abnormally elevated LFTs were also improved more rapidly when compared to other group which was not given ascorbic acid supplements for the period of one month. There was a significant change in levels of some liver function parameters before and after intake of ascorbic acid supplementation, and various abnormally elevated LFTs were also improved when compared to other group which was not given ascorbic acid supplements for the period of one month. The effect of Vitamin C supplementation was more marked on serum aminotransferase levels. After one-month use of ascorbic acid, serum alanine aminotransferase (p < 0.042) and serum aspartate aminotransferase (p < 0.000) levels were significantly decreased in hepatitis C patient group. In HCV group with ascorbic acid supplementation, serum total bilirubin (p < 0.046) and serum direct bilirubin (p < 0.048) were found to be less than the pre values when compared to HCV group without ascorbic acid supplementation. It was also observed that some of protein values were suggestively improved after intake of ascorbic acid supplementation.展开更多
[目的]观察补肺益肾方对肺肾气虚型慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者肺康复的作用。[方法]搜集2021年10月至2023年1月浙江医院确诊的肺肾气虚型COPD稳定期患者55例,随机分成试验组(26例)和对照...[目的]观察补肺益肾方对肺肾气虚型慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者肺康复的作用。[方法]搜集2021年10月至2023年1月浙江医院确诊的肺肾气虚型COPD稳定期患者55例,随机分成试验组(26例)和对照组(29例)。对照组予稳定期吸入剂+肺康复运动训练治疗方案,试验组在此基础上予补肺益肾方加减治疗。评估两组患者治疗前,治疗第4周、第8周的肺功能指标第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)、第1秒用力呼气容积占预计值百分比(forced expiratory volume in the first second to predicted values,FEV1%)、第一秒用力呼气容积占用力肺活量比值(forced expiratory volume in one second to forced vital capacity,FEV1/FVC)、慢性阻塞性肺疾病评估测试(chronic obstructive pulmonary disease assessment test,CAT)评分、咳嗽能力评分、咳痰难度评分、体质量指数(body mass index,BMI),并监测患者不良反应情况。[结果]与本组治疗前比较,两组在治疗4周、8周,FEV1、FEV1%均有升高,CAT评分、咳嗽难度均有下降,差异均有统计学意义(P<0.05);BMI、咳嗽能力差异无统计学意义(P>0.05);试验组FEV1/FVC治疗4周差异无统计学意义(P>0.05),治疗8周升高,差异有统计学意义(P<0.05)。与对照组同期比较,试验组在治疗4周、8周,CAT评分降低、咳嗽难度下降,差异均有统计学意义(P<0.05),FEV1、FEV1%、咳嗽能力、BMI差异均无统计学意义(P>0.05);试验组FEV1/FVC在治疗4周差异无统计学意义(P>0.05),治疗8周升高,差异有统计学意义(P<0.05)。[结论]补肺益肾方在COPD稳定期患者中对肺康复起到协同作用。展开更多
Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. Current treatments often involve synthetic drugs with significant side effects a...Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. Current treatments often involve synthetic drugs with significant side effects and potential for dependency. This study investigates the effects of a natural supplement combination of Ginkgo Biloba and Acai Extract on cognitive symptoms in a 77-year-old male with PD. The participant underwent a three-month supplementation regimen, with cognitive function assessed using the Montreal Cognitive Assessment (MoCA) test before and after the intervention. The results indicated an improvement in cognitive scores, suggesting that the combination of Ginkgo Biloba and Acai Extract may offer a promising alternative or adjunct to conventional PD treatments. This study highlights the potential of natural supplements in managing PD symptoms and calls for further research with larger sample sizes to confirm these findings. Human data was performed in accordance with the Declaration of Helsinki by the Roxbury District IRB Board (IRB Number: IRB00011767).展开更多
Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on ...Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.展开更多
文摘Pearl millet cultivar (Dempy) was supplemented with soybean flour to raise the protein content by 5%, 10% and 15%. Defatted soybean flour, pearl millet flour and their composite flour had varying functional properties. Water and fat absorption, bulk density, nitrogen solubility and dispersibility were higher for soybean flour compared to that of pearl millet flour. Therefore, such properties were improved significantly (P ≤ 0.05) for pearl millet with increasing the level of soybean supplementation. The emulsifying and foaming properties of pearl millet flour were poor but after supplementation with soybean they significantly (P ≤ 0.05) improved. Supplementation of pearl millet flour with soybean had no effect on least gelation concentration of millet. The implication of these results will be realized in designing protein-enriched products based on pearl millet flour, especially for pearl millet–growing regions in the under-developing countries.
文摘An isolated liver function test is of little role in selection of liver disease because many harmful liver diseases may be correlated with normal levels of LFT’s. The outline of enzyme abnormalities in the perspective of patient’s commonly observed symptoms and laboratory data might be helpful in directing the subsequent diagnosis of liver diseases. Liver Function Tests (LFTs) are most generally used screening blood tests for assessment of different liver diseases and these tests provide a lot of evidence for disease processes whether for the purpose of investigation of supposed liver disease or help in observing the progress of disease action or simply by blood investigation. The evaluation of different liver enzymes simply gives diagnostic information on basic level whether patient’s principal disorder is actually hepatitis or cholestasis in source. However, it is necessary in various cases to evaluate LFTs with knowledge of liver functioning enzyme fractions. The objective of this study was to explore the effects of ascorbic acid supplementation on serum liver function tests in Hepatitis C patients. A total of 100 hepatitis C patients were selected randomly. 50 were given ascorbic acid supplementation for one month along with anti HCV treatment. The other 50 HCV patients took their normal anti HCV treatment without intake of ascorbic acid supplementation, and serum ascorbic acid level and liver function test parameters were observed before and after intake of ascorbic acid in both groups. The liver function parameters determined were aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), serum total bilirubin, direct bilirubin, indirect bilirubin and serum protein (total protein, albumin, globulin and A/G ratio). These parameters along with serum ascorbic acid were measured before and 30 days after vitamin C supplementation. Various abnormally elevated LFTs were also improved more rapidly when compared to other group which was not given ascorbic acid supplements for the period of one month. There was a significant change in levels of some liver function parameters before and after intake of ascorbic acid supplementation, and various abnormally elevated LFTs were also improved when compared to other group which was not given ascorbic acid supplements for the period of one month. The effect of Vitamin C supplementation was more marked on serum aminotransferase levels. After one-month use of ascorbic acid, serum alanine aminotransferase (p < 0.042) and serum aspartate aminotransferase (p < 0.000) levels were significantly decreased in hepatitis C patient group. In HCV group with ascorbic acid supplementation, serum total bilirubin (p < 0.046) and serum direct bilirubin (p < 0.048) were found to be less than the pre values when compared to HCV group without ascorbic acid supplementation. It was also observed that some of protein values were suggestively improved after intake of ascorbic acid supplementation.
文摘[目的]观察补肺益肾方对肺肾气虚型慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者肺康复的作用。[方法]搜集2021年10月至2023年1月浙江医院确诊的肺肾气虚型COPD稳定期患者55例,随机分成试验组(26例)和对照组(29例)。对照组予稳定期吸入剂+肺康复运动训练治疗方案,试验组在此基础上予补肺益肾方加减治疗。评估两组患者治疗前,治疗第4周、第8周的肺功能指标第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)、第1秒用力呼气容积占预计值百分比(forced expiratory volume in the first second to predicted values,FEV1%)、第一秒用力呼气容积占用力肺活量比值(forced expiratory volume in one second to forced vital capacity,FEV1/FVC)、慢性阻塞性肺疾病评估测试(chronic obstructive pulmonary disease assessment test,CAT)评分、咳嗽能力评分、咳痰难度评分、体质量指数(body mass index,BMI),并监测患者不良反应情况。[结果]与本组治疗前比较,两组在治疗4周、8周,FEV1、FEV1%均有升高,CAT评分、咳嗽难度均有下降,差异均有统计学意义(P<0.05);BMI、咳嗽能力差异无统计学意义(P>0.05);试验组FEV1/FVC治疗4周差异无统计学意义(P>0.05),治疗8周升高,差异有统计学意义(P<0.05)。与对照组同期比较,试验组在治疗4周、8周,CAT评分降低、咳嗽难度下降,差异均有统计学意义(P<0.05),FEV1、FEV1%、咳嗽能力、BMI差异均无统计学意义(P>0.05);试验组FEV1/FVC在治疗4周差异无统计学意义(P>0.05),治疗8周升高,差异有统计学意义(P<0.05)。[结论]补肺益肾方在COPD稳定期患者中对肺康复起到协同作用。
文摘Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. Current treatments often involve synthetic drugs with significant side effects and potential for dependency. This study investigates the effects of a natural supplement combination of Ginkgo Biloba and Acai Extract on cognitive symptoms in a 77-year-old male with PD. The participant underwent a three-month supplementation regimen, with cognitive function assessed using the Montreal Cognitive Assessment (MoCA) test before and after the intervention. The results indicated an improvement in cognitive scores, suggesting that the combination of Ginkgo Biloba and Acai Extract may offer a promising alternative or adjunct to conventional PD treatments. This study highlights the potential of natural supplements in managing PD symptoms and calls for further research with larger sample sizes to confirm these findings. Human data was performed in accordance with the Declaration of Helsinki by the Roxbury District IRB Board (IRB Number: IRB00011767).
基金funded by Regione Emilia-Romagna POR-FESR 2014-2020"Innovare la filiera suina mediante la valorizzazione di sottoprodotti vegetali e l’impiego di avanzate tecnologie "omiche" e di processo,per la produzione sostenibile di carne e salumi ad impatto positivo sulla salute"-Green Charcuterie grant n.PG/2015/730542
文摘Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.
