In this study,an improved integrated radial basis function with nonuniform shape parameter is introduced.The proposed shape parameter varies in each support domain and is defined byθ=1/d_(max),where d_(max)is the max...In this study,an improved integrated radial basis function with nonuniform shape parameter is introduced.The proposed shape parameter varies in each support domain and is defined byθ=1/d_(max),where d_(max)is the maximum distance of any pair of nodes in the support domain.The proposed method is verified and shows good performance.The results are stable and accurate with any number of nodes and an arbitrary nodal distribution.Notably,the support domain should be large enough to obtain accurate results.This method is then applied for transient analysis of curved shell structures made from functionally graded materials with complex geometries.Through several numerical examples,the accuracy of the proposed approach is demonstrated and discussed.Additionally,the influence of various factors on the dynamic behavior of the structures,including the power-law index,different materials,loading conditions,and geometrical parameters of the structures,was investigated.展开更多
By adopting stochastic density functional theory(SDFT)and mixed stochastic-deterministic density functional theory(MDFT)methods,we perform first-principles calculations to predict the shock Hugoniot curves of boron(pr...By adopting stochastic density functional theory(SDFT)and mixed stochastic-deterministic density functional theory(MDFT)methods,we perform first-principles calculations to predict the shock Hugoniot curves of boron(pressure P=7.9×10^(3)-1.6×10^(6) GPa and temperature T=25-2800 eV),silicon(P=2.6×10^(3)-7.9×10^(5) GPa and T=21.5-1393 eV),and aluminum(P=5.2×10^(3)-9.0×10^(5) GPa and T=25-1393 eV)over wide ranges of pressure and temperature.In particular,we systematically investigate the impact of different cutoff radii in norm-conserving pseudopotentials on the calculated properties at elevated temperatures,such as pressure,ionization energy,and equation of state.By comparing the SDFT and MDFT results with those of other first-principles methods,such as extended first-principles molecular dynamics and path integral Monte Carlo methods,we find that the SDFT and MDFT methods show satisfactory precision,which advances our understanding of first-principles methods when applied to studies of matter at extremely high pressures and temperatures.展开更多
Curved beams with complex geometries are vital in numerous engineering applications,where precise vibration analysis is crucial for ensuring safe and effective designs.Traditional finite element methods(FEMs) often st...Curved beams with complex geometries are vital in numerous engineering applications,where precise vibration analysis is crucial for ensuring safe and effective designs.Traditional finite element methods(FEMs) often struggle to accurately represent the dynamic characteristics of these structures due to the limitations in their shape function approximations.To overcome this challenge,the current study introduces an innovative finite element(FE)-based technique for the undamped vibrational analysis of curved beams with arbitrary curvature,employing explicitly derived interpolation functions.Initially,the exact interpolation functions are developed for circular are elements with the force method.These functions facilitate the creation of a highly accurate stiffness matrix,which is validated against the benchmark examples.To accommodate arbitrary curvature,a systematic transformation technique is established to approximate the intricate curves with a series of circular arcs.The numerical findings indicate that increasing the number of arc segments enhances accuracy,approaching the exact solutions.The analysis of free vibrations is conducted for both circular and non-circular beams.Mass matrices are derived using two methods:lumped mass and consistent mass,where the latter is based on the interpolation functions.The effectiveness of the proposed method is confirmed through the comparisons with the existing literature,demonstrating strong agreement.Finally,several practical cases involving beams with diverse curvature profiles are analyzed.Both natural frequencies and mode shapes are determined,providing significant insights into the dynamic behavior of these structures.This research offers a dependable and efficient analytical framework for the vibrational analysis of complex curved beams,with promising implications for structural and mechanical engineering.展开更多
This study investigates the properties of high-purity starches extracted from Polygonum multiflorum(PMS)and Smilax glabra(SGS).The starches were characterized by scanning electron microscopy,Fouriertransform infrared ...This study investigates the properties of high-purity starches extracted from Polygonum multiflorum(PMS)and Smilax glabra(SGS).The starches were characterized by scanning electron microscopy,Fouriertransform infrared spectroscopy,X-ray diffraction,high-performance anion-exchange chromatography,and differential scanning calorimetry.Significant differences were observed in their morphological,physicochemical,and functional properties.PMS had a smaller particle size(13.68 μm),irregular polygonal shape,A-type,lower water absorption(62.67 %),and higher oil absorption(51.17 %).In contrast,SGS exhibited larger particles(31.75 μm),a nearly spherical shape,B-type,higher crystallinity(50.66 %),and greater amylose content(21.54 %),with superior thermal stability,shear resistance,and gelatinization enthalpy.SGS also contained higher resistant starch(83.28 %) and longer average chain length(20.58 %),but showed lower solubility,swelling power,light transmittance,and freeze-thaw stability.The physicochemical properties differences in crystal pattern and particle morphology between PMS and SGS lead to distinct behaviors during in vitro digestion and fermentation.These findings highlight the potential of medicinal plant starches in functional ingredients and industrial processes.展开更多
Gauss radial basis functions(GRBF)are frequently employed in data fitting and machine learning.Their linear independence property can theoretically guarantee the avoidance of data redundancy.In this paper,one of the m...Gauss radial basis functions(GRBF)are frequently employed in data fitting and machine learning.Their linear independence property can theoretically guarantee the avoidance of data redundancy.In this paper,one of the main contributions is proving this property using linear algebra instead of profound knowledge.This makes it easy to read and understand this fundamental fact.The proof of linear independence of a set of Gauss functions relies on the constructing method for one-dimensional space and on the deducing method for higher dimensions.Additionally,under the condition of preserving the same moments between the original function and interpolating function,both the interpolating existence and uniqueness are proven for GRBF in one-dimensional space.The final work demonstrates the application of the GRBF method to locate lunar olivine.By combining preprocessed data using GRBF with the removing envelope curve method,a program is created to find the position of lunar olivine based on spectrum data,and the numerical experiment shows that it is an effective scheme.展开更多
Spinal cord injury represents a severe form of central nervous system trauma for which effective treatments remain limited.Microglia is the resident immune cells of the central nervous system,play a critical role in s...Spinal cord injury represents a severe form of central nervous system trauma for which effective treatments remain limited.Microglia is the resident immune cells of the central nervous system,play a critical role in spinal cord injury.Previous studies have shown that microglia can promote neuronal survival by phagocytosing dead cells and debris and by releasing neuroprotective and anti-inflammatory factors.However,excessive activation of microglia can lead to persistent inflammation and contribute to the formation of glial scars,which hinder axonal regeneration.Despite this,the precise role and mechanisms of microglia during the acute phase of spinal cord injury remain controversial and poorly understood.To elucidate the role of microglia in spinal cord injury,we employed the colony-stimulating factor 1 receptor inhibitor PLX5622 to deplete microglia.We observed that sustained depletion of microglia resulted in an expansion of the lesion area,downregulation of brain-derived neurotrophic factor,and impaired functional recovery after spinal cord injury.Next,we generated a transgenic mouse line with conditional overexpression of brain-derived neurotrophic factor specifically in microglia.We found that brain-derived neurotrophic factor overexpression in microglia increased angiogenesis and blood flow following spinal cord injury and facilitated the recovery of hindlimb motor function.Additionally,brain-derived neurotrophic factor overexpression in microglia reduced inflammation and neuronal apoptosis during the acute phase of spinal cord injury.Furthermore,through using specific transgenic mouse lines,TMEM119,and the colony-stimulating factor 1 receptor inhibitor PLX73086,we demonstrated that the neuroprotective effects were predominantly due to brain-derived neurotrophic factor overexpression in microglia rather than macrophages.In conclusion,our findings suggest the critical role of microglia in the formation of protective glial scars.Depleting microglia is detrimental to recovery of spinal cord injury,whereas targeting brain-derived neurotrophic factor overexpression in microglia represents a promising and novel therapeutic strategy to enhance motor function recovery in patients with spinal cord injury.展开更多
This study aims to explore the impact of fatigue induced by different limb exercises on cerebral cortical oxygenation levels and functional connectivity strength using functional near-infrared spectroscopy(fNIRS).Fati...This study aims to explore the impact of fatigue induced by different limb exercises on cerebral cortical oxygenation levels and functional connectivity strength using functional near-infrared spectroscopy(fNIRS).Fatigue was induced using an upper limb ergometer or a lower limb ergometer,with the load increasing gradually each minute.fNIRS covering the prefrontal cortex and motor cortex were used to collect data during the resting state,both before and after fatigue induction.A two-way ANOVA was conducted to examine differences in oxyhemoglobin(HbO_(2))and functional connectivity before and after fatigue induction in both groups,with the significance level set at 0.05.Exercise-induced fatigue in both the upper and lower limbs leads to a significant decrease in cerebral cortical oxygenation levels.Upper limb fatigue leads to a significant reduction in functional connectivity,there were significant decreases in connectivity within the motor cortex,between the motor cortex and frontal regions,and between the right ventrolateral prefrontal cortex and other frontal regions.Conversely,no significant changes were observed before and after lower limb fatigue.Future studies should focus on examining the extent to which how changes in the cerebral cortex,induced by exercise fatigue,are linked to exercise-and/or performance-related outcomes.展开更多
In this paper,the isogeometric analysis(IGA)method is employed to analyze the oscillation characteristics of functionally graded triply periodic minimal surface(FG-TPMS)curved-doubly shells integrated with magneto-ele...In this paper,the isogeometric analysis(IGA)method is employed to analyze the oscillation characteristics of functionally graded triply periodic minimal surface(FG-TPMS)curved-doubly shells integrated with magneto-electric surface layers(referred to as"FG-TPMS-MEE curved-doubly shells")subjected to low-velocity impact loads.This study presents low-velocity impact load model based on a single springmass(S-M)approach.The FG-TPMS-MEE curved-doubly shells are covered with two magneto-electric surface layers,while the core layer consists of three types:I-graph and Wrapped Package-graph(IWP),Gyroid(G),and Primitive(P),with various graded functions.These types are notable for their exceptional stiffness-to-weight ratios,enabling a wide range of potential applications.The Maxwell equations and electromagnetic boundary conditions are applied to compute the change in electric potentials and magnetic potentials.The equilibrium equations of the shell are derived from a refined higher-order shear deformation theory(HSDT),and the transient responses of the FG-TPMS-MEE curveddoubly shells are subsequently determined using Newmark's direct integration method.These results have applications in structural vibration control and the analysis of structures subjected to impact or explosive loads.Furthermore,this study provides a theoretical prediction of the low-velocity impact load and magneto-electric-elastic effects on the free vibration and transient response of FG-TPMS-MEE curved-doubly shells.展开更多
Functional gastrointestinal disorders(FGIDs),including irritable bowel syndrome(IBS),functional dyspepsia(FD),and gastroesophageal reflux disease(GERD),present persistent diagnostic and therapeutic challenges due to s...Functional gastrointestinal disorders(FGIDs),including irritable bowel syndrome(IBS),functional dyspepsia(FD),and gastroesophageal reflux disease(GERD),present persistent diagnostic and therapeutic challenges due to symptom heterogeneity and the absence of reliable biomarkers.Artificial intelligence(AI)enables the integration of multimodal data to enhance FGID management through precision diagnostics and preventive healthcare.This minireview summarizes recent advancements in AI applications for FGIDs,highlighting progress in diagnostic accuracy,subtype classification,personalized interventions,and preventive strategies inspired by the traditional Chinese medicine concept of“treating the undiseased”.Machine learning and deep learning algorithms have demonstrated value in improving IBS diagnosis,refining FD neuro-gastrointestinal subtyping,and screening for GERD-related complications.Moreover,AI supports dietary,psychological,and integrative medicine-based interventions to improve patient adherence and quality of life.Nonetheless,key challenges remain,including data heterogeneity,limited model interpretability,and the need for robust clinical validation.Future directions emphasize interdisciplinary collaboration,the development of multimodal and explainable AI models,and the creation of patientcentered platforms to facilitate a shift from reactive treatment to proactive prevention.This review provides a systematic framework to guide the clinical application and theoretical innovation of AI in FGIDs.展开更多
The capillary pressure curve provides fundamental insights into the dynamics of fluid-fluid displacement and phase distributions.Capillary scaling is crucial for extrapolating capillary pressure-saturation data from l...The capillary pressure curve provides fundamental insights into the dynamics of fluid-fluid displacement and phase distributions.Capillary scaling is crucial for extrapolating capillary pressure-saturation data from laboratory tests to field applications.However,the classic scaling method fails to capture the effect of wettability as the pore surface approaches neutral wetting.Here,inspired by the role of pore-filling events in controlling fluid-fluid displacement,we perform a theoretical analysis of the burst events occurring during drainage processes.We find that the median threshold capillary pressure,which corresponds to the occurrence of burst events for the median pore throat,is closely correlated with the capillary pressure curve across various contact angles.Using this concept,we propose a new scaling method for capillary pressure curves under various wetting conditions.We conduct microfluidic experiments and pore-network modeling across different contact angles,porosities,and disorders to evaluate the new scaling methods,indicating that the new scaling method performs better than the Leverett J-function as the contact angle approaches 90°.We further perform geometry analysis on the critical radius of curvature for burst events in an ideal tetrahedral arrangement and extend the new scaling method to 3D(three-dimensional)porous media.Model evaluation shows that the 3D version of the scaling method also performs well but requires fewer parameters compared to the Leverett J-function.Our work enhances the prediction and interpretation of experimental data for capillary pressure curves under various wet conditions,and more importantly,establishes a methodology that relates Darcy-scale flow behavior to pore-scale fluid displacements.展开更多
Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural pro...Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural products,thereby endangering human health.Extensive studies on Aspergillus fungi have been conducted on growth and development,aflatoxin biosynthesis,and their interactions with environment.Here,we summarized a series of functional genes of the main Aspergillus fungi relative to toxins occurrence in foods,which revealed the signal transduction mechanisms of their involvement in growth and development,toxin production,and response to light,anticipating providing theoretical guidance on developing control and prevention technologies for mycotoxin contamination in agricultural products to ensure food safety.展开更多
Given the broad applicability of carbazole structural moieties in materials science and medicinal chemistry,significant efforts have been devoted to developing efficient synthetic catalytic methodologies to access thi...Given the broad applicability of carbazole structural moieties in materials science and medicinal chemistry,significant efforts have been devoted to developing efficient synthetic catalytic methodologies to access this valuable scaffold.Catalyzed direct Csp^(2)-H functionalization provides an effective and costefficient approach to synthesizing carbazoles from simple and readily available starting materials,ensuring a promising path characterized by excellent atom and step economy.This review highlights the substantial progress made in the last 10 years in advancing catalytic Csp^(2)-H functionalization techniques for synthesizing carbazoles.展开更多
Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear ...Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear and contentious.This study offered a comprehensive review and meta-analysis of research published until June 2024,analyzing the effects of multivitamins on various cognitive functions in individuals aged 65 and older.We included ten randomized controlled trials encompassing 13,600 participants from multiple databases.These studies evaluated the impact of multivitamins on reasoning,memory,learning,visual perception,idea production,cognitive speed,psychomotor abilities,and higher cognitive functions.Our meta-analysis revealed that multivitamins significantly enhanced delayed free recall(SMD=0.09,95%confidence interval(CI)=[0.05,0.13],P=0.0001).However,they had no substantial effects on immediate free recall(SMD=0.85,95%CI=[-0.1,1.9],P=0.11),idea production(SMD=0.00,95%CI=[-0.04,0.03],P=0.86),or cognitive functioning(SMD=0.07,94%CI=[-0.07,0.14],P=0.006).Thus,while multivitamins facilitated delayed free recall,they did not significantly improve other cognitive functions in older adults.展开更多
BACKGROUND An echocardiogram is an essential tool in the evaluation of potential kidney transplant recipients(KTRs).Despite cardiac clearance,potential KTRs still have structural and functional abnormalities.Identifyi...BACKGROUND An echocardiogram is an essential tool in the evaluation of potential kidney transplant recipients(KTRs).Despite cardiac clearance,potential KTRs still have structural and functional abnormalities.Identifying the prevalence of these abnormalities and understanding their predictors is vital for optimizing pretransplant risk stratification and improving post-transplant outcomes.AIM To determine the prevalence of left ventricular hypertrophy(LVH),left ventricular systolic dysfunction(LVSD),diastolic dysfunction(DD),pulmonary hypertension(PH),and their predictors,and to assess their impact on graft function in pre-transplant candidates.METHODS The study included all successful transplant candidates older than 14 who had a baseline echocardiogram.Binary logistic regression models were constructed to identify factors associated with LVH,LVSD,DD,and PH.RESULTS Out of 259 patients,LVH was present in 64%(166),12%(31)had LVSD,27.5%(71)had DD,and 66(25.5%)had PH.Independent predictors of LVH included male gender[odds ratio(OR):2.51;95%CI:1.17-5.41 P=0.02],PH(OR=2.07;95%CI:1.11-3.86;P=0.02),DD(OR:2.47;95%CI:1.29-4.73;P=0.006),and dyslipidemia(OR=1.94;95%CI:1.07-3.53;P=0.03).Predictors for LVSD included patients with DD(OR=3.3,95%CI:1.41-7.81;P=0.006)and a family history of coronary artery disease(OR=4.50,95%CI:1.33-15.20;P=0.015).Peritoneal dialysis was an independent predictor for DD(OR=10.03;95%CI:1.71-58.94,P=0.011).The presence of LVH(OR=3.32,95%CI:1.05-10.55,P=0.04)and mild to moderate or moderate to severe mitral regurgitation(OR=4.63,95%CI:1.45-14.78,P=0.01)were significant factors associated with PH.These abnormalities had no significant impact on estimated glomerular filtration at discharge,6 months,1 year,or 2 years post-transplant.CONCLUSION Significant echocardiographic abnormalities persist in a potential transplant candidate despite cardiac clearance,although they don’t affect future graft function.Understanding the risk factors associated with these abnormalities may help clinicians address these factors pre-and post-transplant to achieve better outcomes.展开更多
The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic fi...The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic field.These microorganisms produce density gradients by swimming,which induces macroscopic convection flows in the fluid.This procedure improves the mass and heat transfer,illustrating the interaction between biological activity and fluid dynamics.Furthermore,instead of considering traditional Fourier's and Fick's law the energy and concentration equations are developed by incorporating Cattaneo-Christov double diffusion theory.Moreover,to examine the influence of thermophoresis and Brownian diffusions in the fluid we have adopted the Buongiorno nanofluid model.Due to the oscillation of the surface of the channel,the mathematical development of the considered flow problem is obtained in the form of partial differential equations via the curvilinear coordinate system.The convergent series solution of the governing flow equations is obtained after applying the homotopy analysis method(HAM).The effects of different pertinent flow parameters on velocity,motile microorganism density distribution,concentration,pressure,temperature,and skin friction coefficient are examined and discussed in detail with the help of graphs and tables.It is observed during the current study that the density of microorganisms is enhanced for higher values of Reynolds number,Peclet number,radius of curvature variable,and Lewis number.展开更多
[Objectives]To investigate the efficacy and potential mechanism of the topical preparation Jineijin-Shanzha Patch(composed of Galli Gigerii Endothelium Corneum and Crataegi Fructus)in improving functional dyspepsia(FD...[Objectives]To investigate the efficacy and potential mechanism of the topical preparation Jineijin-Shanzha Patch(composed of Galli Gigerii Endothelium Corneum and Crataegi Fructus)in improving functional dyspepsia(FD)based on network pharmacology.[Methods]Firstly,we reviewed the existing research progress on each constituent drug of the Jineijin Shanzha Patch for FD improvement.Following this,identified overlapping genes were utilized to construct both a"Drug-Active Component-FD Target"network and a Protein-Protein Interaction(PPI)network specific to the patch.In addition,Gene Ontology(GO)analysis was carried out.[Results]We identified that the 13 herbs in the Jineijin Shanzha Patch are mainly used for food stagnation,qi stagnation,and spleen deficiency.Screening revealed 43 active patch components,1414 FD-related targets,and 284 shared targets between them.The PPI network analysis further identified the top 10 core targets from these shared targets.From the"Drug-Active Component-FD Target"network,we identified the core elements.These included the herbal components Vignae Semen(from Liushenqu),Crataegi Fructus,and Pseudostellariae Radix;the active components quercetin,genistein,and apigenin;and the key targets CASP3,BCL2,and CASP9.GO analysis of the 284 overlapping targets indicated that the Jineijin Shanzha Patch may exert its therapeutic effects via regulation of biological processes such as the response to lipopolysaccharide,response to bacterium-derived molecules,and regulation of the apoptotic signaling pathway.[Conclusions]The main active components of the Jineijin Shanzha Patch(quercetin,genistein,and apigenin)may improve FD by modulating signaling pathways such as the response to lipopolysaccharide,response to bacterium-derived molecules,and regulation of the apoptotic signaling pathway,thereby acting on key targets including CASP3,BCL2,and CASP9.展开更多
Background:Targeted delivery of biological macromolecules to the small intestine remains challenging due to their susceptibility to degradation in the hostile gastric environment.Methods:This study introduces a minima...Background:Targeted delivery of biological macromolecules to the small intestine remains challenging due to their susceptibility to degradation in the hostile gastric environment.Methods:This study introduces a minimally invasive,in situ injection technique for the murine small intestine that facilitates localized luminal delivery while circumventing gastric barriers.The procedure involves a small abdominal incision for direct injection into the duodenum near the pylorus.Postsurgical monitoring of physiological parameters,systemic inflammatory markers,liver function,and intestinal integrity was conducted over 72 h.Histopathological analysis was performed.The delivery of the functional protein TAT-EGFP(Tat protein fused to enhanced green fluorescent protein)to intestinal epithelial cells was evaluated and compared with oral gavage.As a proof of concept,single-cell RNA sequencing of the intestinal epithelium was performed after high-mobility group box 1 administration.Results:Postsurgical monitoring indicated only transient,anesthesia-related hypo-thermia and minor behavioral alterations.No significant changes were observed over 72 h in body weight,core temperature,clinical severity scores,systemic inflammatory markers(C-reactive protein and leukocytes),liver function(alanine aminotransferase),or intestinal integrity.Histopathological analysis confirmed preserved tissue architec-ture and normal digestive,absorptive,and barrier functions.The model successfully delivered TAT-EGFP to intestinal epithelial cells,an outcome not achievable via oral gavage due to gastric degradation.Single-cell RNA sequencing of the intestinal epi-thelium after high-mobility group box 1 administration revealed inflammatory gene expression patterns in specific epithelial subpopulations.Conclusions:Compared to traditional methods such as oral gavage or organoid cul-ture,this technique offers precise,degradation-resistant delivery of macromolecules in a physiological context.The model's versatility makes it a powerful platform for intestinal research,with applications in drug delivery assessment,gene therapy evalu-ation,and host-microbiota interaction studies.展开更多
The original online version of this article was revised:The layout update for Article 758 has impacted the page range in the published issue,but did not affect the scholarly content.To ensure consistency with the orig...The original online version of this article was revised:The layout update for Article 758 has impacted the page range in the published issue,but did not affect the scholarly content.To ensure consistency with the originally assigned pages(2595-2614),we will need to publish an erratum to correct the article and restore the original page range.The original article has been corrected.展开更多
Ln@MOFs by anchoring rare metal ions(Ln) into metal-organic frameworks(MOFs) are proved to have great potential in the field of luminescent molecular thermometer.Nevertheless,the current research indicated that the po...Ln@MOFs by anchoring rare metal ions(Ln) into metal-organic frameworks(MOFs) are proved to have great potential in the field of luminescent molecular thermometer.Nevertheless,the current research indicated that the poor structural stability and low sensitivity hindered their application scope.In this work,a new MOF Zn-450 luminescent thermometer with multiple emission fluorescence characteristics was synthesized by the combination of 3,3,5,5-biphenyl tetracarboxylic acid(H_(4)L) and Zn^(2+) ion under solvothermal conditions.Interestingly,a high relative sensitivity of 1.43 % K^(-1) was found within 80-300 K based on Zn-450.Subsequently,two high-sensitivity luminescent Ln@MOFs(Ln = Eu and Tb) were further fabricated by doping rare earth ions into Zn-450 based on the post-synthesis strategy.Among them,the Eu@Zn-450 demonstrates various luminous behaviors while achieving an increased relative sensitivity of 1.63 % K^(-1).In addition,the continuously visible red,pink,and purple luminescent emissions at the same temperature range were observed,suggesting that the Eu@Zn-450 could be utilized as a luminescent colorimetric molecular thermometer.Importantly,this work can present new possibilities for the development of rare earth-doped luminescence and its temperature sensing properties.展开更多
BACKGROUND Suicide constitutes the second leading cause of death among adolescents globally and represents a critical public health concern.The neural mechanisms underlying suicidal behavior in adolescents with major ...BACKGROUND Suicide constitutes the second leading cause of death among adolescents globally and represents a critical public health concern.The neural mechanisms underlying suicidal behavior in adolescents with major depressive disorder(MDD)remain poorly understood.Aberrant resting-state functional connectivity(rsFC)in the amygdala,a key region implicated in emotional regulation and threat detection,is strongly implicated in depression and suicidal behavior.AIM To investigate rsFC alterations between amygdala subregions and whole-brain networks in adolescent patients with depression and suicide attempts.METHODS Resting-state functional magnetic resonance imaging data were acquired from 32 adolescents with MDD and suicide attempts(sMDD)group,33 adolescents with MDD but without suicide attempts(nsMDD)group,and 34 demographically matched healthy control(HC)group,with the lateral and medial amygdala(MeA)defined as regions of interest.The rsFC patterns of amygdala subregions were compared across the three groups,and associations between aberrant rsFC values and clinical symptom severity scores were examined.RESULTS Compared with the nsMDD group,the sMDD group exhibited reduced rsFC between the right lateral amygdala(LA)and the right inferior occipital gyrus as well as the left middle occipital gyrus.Compared with the HC group,the abnormal brain regions of rsFC in the sMDD group and nsMDD group involve the parahippocampal gyrus(PHG)and fusiform gyrus.In the sMDD group,right MeA and right temporal pole:Superior temporal gyrus rsFC value negatively correlated with the Rosenberg Self-Esteem Scale scores(r=-0.409,P=0.025),while left LA and right PHG rsFC value positively correlated with the Adolescent Self-Rating Life Events Checklist interpersonal relationship scores(r=0.372,P=0.043).CONCLUSION Aberrant rsFC changes between amygdala subregions and these brain regions provide novel insights into the underlying neural mechanisms of suicide attempts in adolescents with MDD.展开更多
基金Ho Chi Minh City University of Technology (HCMUT), VNU-HCM for supporting this study
文摘In this study,an improved integrated radial basis function with nonuniform shape parameter is introduced.The proposed shape parameter varies in each support domain and is defined byθ=1/d_(max),where d_(max)is the maximum distance of any pair of nodes in the support domain.The proposed method is verified and shows good performance.The results are stable and accurate with any number of nodes and an arbitrary nodal distribution.Notably,the support domain should be large enough to obtain accurate results.This method is then applied for transient analysis of curved shell structures made from functionally graded materials with complex geometries.Through several numerical examples,the accuracy of the proposed approach is demonstrated and discussed.Additionally,the influence of various factors on the dynamic behavior of the structures,including the power-law index,different materials,loading conditions,and geometrical parameters of the structures,was investigated.
基金supported by the National Key R&D Program of China under Grant No.2025YFB3003603the National Natural Science Foundation of China under Grant Nos.12135002 and 12105209.
文摘By adopting stochastic density functional theory(SDFT)and mixed stochastic-deterministic density functional theory(MDFT)methods,we perform first-principles calculations to predict the shock Hugoniot curves of boron(pressure P=7.9×10^(3)-1.6×10^(6) GPa and temperature T=25-2800 eV),silicon(P=2.6×10^(3)-7.9×10^(5) GPa and T=21.5-1393 eV),and aluminum(P=5.2×10^(3)-9.0×10^(5) GPa and T=25-1393 eV)over wide ranges of pressure and temperature.In particular,we systematically investigate the impact of different cutoff radii in norm-conserving pseudopotentials on the calculated properties at elevated temperatures,such as pressure,ionization energy,and equation of state.By comparing the SDFT and MDFT results with those of other first-principles methods,such as extended first-principles molecular dynamics and path integral Monte Carlo methods,we find that the SDFT and MDFT methods show satisfactory precision,which advances our understanding of first-principles methods when applied to studies of matter at extremely high pressures and temperatures.
文摘Curved beams with complex geometries are vital in numerous engineering applications,where precise vibration analysis is crucial for ensuring safe and effective designs.Traditional finite element methods(FEMs) often struggle to accurately represent the dynamic characteristics of these structures due to the limitations in their shape function approximations.To overcome this challenge,the current study introduces an innovative finite element(FE)-based technique for the undamped vibrational analysis of curved beams with arbitrary curvature,employing explicitly derived interpolation functions.Initially,the exact interpolation functions are developed for circular are elements with the force method.These functions facilitate the creation of a highly accurate stiffness matrix,which is validated against the benchmark examples.To accommodate arbitrary curvature,a systematic transformation technique is established to approximate the intricate curves with a series of circular arcs.The numerical findings indicate that increasing the number of arc segments enhances accuracy,approaching the exact solutions.The analysis of free vibrations is conducted for both circular and non-circular beams.Mass matrices are derived using two methods:lumped mass and consistent mass,where the latter is based on the interpolation functions.The effectiveness of the proposed method is confirmed through the comparisons with the existing literature,demonstrating strong agreement.Finally,several practical cases involving beams with diverse curvature profiles are analyzed.Both natural frequencies and mode shapes are determined,providing significant insights into the dynamic behavior of these structures.This research offers a dependable and efficient analytical framework for the vibrational analysis of complex curved beams,with promising implications for structural and mechanical engineering.
基金supported by the National Natural Science Foundation of China (No.82174074)。
文摘This study investigates the properties of high-purity starches extracted from Polygonum multiflorum(PMS)and Smilax glabra(SGS).The starches were characterized by scanning electron microscopy,Fouriertransform infrared spectroscopy,X-ray diffraction,high-performance anion-exchange chromatography,and differential scanning calorimetry.Significant differences were observed in their morphological,physicochemical,and functional properties.PMS had a smaller particle size(13.68 μm),irregular polygonal shape,A-type,lower water absorption(62.67 %),and higher oil absorption(51.17 %).In contrast,SGS exhibited larger particles(31.75 μm),a nearly spherical shape,B-type,higher crystallinity(50.66 %),and greater amylose content(21.54 %),with superior thermal stability,shear resistance,and gelatinization enthalpy.SGS also contained higher resistant starch(83.28 %) and longer average chain length(20.58 %),but showed lower solubility,swelling power,light transmittance,and freeze-thaw stability.The physicochemical properties differences in crystal pattern and particle morphology between PMS and SGS lead to distinct behaviors during in vitro digestion and fermentation.These findings highlight the potential of medicinal plant starches in functional ingredients and industrial processes.
基金Supported by the National Basic Research Program of China(2012CB025904)Zhengzhou Shengda University of Economics,Business and Management(SD-YB2025085)。
文摘Gauss radial basis functions(GRBF)are frequently employed in data fitting and machine learning.Their linear independence property can theoretically guarantee the avoidance of data redundancy.In this paper,one of the main contributions is proving this property using linear algebra instead of profound knowledge.This makes it easy to read and understand this fundamental fact.The proof of linear independence of a set of Gauss functions relies on the constructing method for one-dimensional space and on the deducing method for higher dimensions.Additionally,under the condition of preserving the same moments between the original function and interpolating function,both the interpolating existence and uniqueness are proven for GRBF in one-dimensional space.The final work demonstrates the application of the GRBF method to locate lunar olivine.By combining preprocessed data using GRBF with the removing envelope curve method,a program is created to find the position of lunar olivine based on spectrum data,and the numerical experiment shows that it is an effective scheme.
基金supported by the National Natural Science Foundation of China,Nos.82072165 and 82272256(both to XM)the Key Project of Xiangyang Central Hospital,No.2023YZ03(to RM)。
文摘Spinal cord injury represents a severe form of central nervous system trauma for which effective treatments remain limited.Microglia is the resident immune cells of the central nervous system,play a critical role in spinal cord injury.Previous studies have shown that microglia can promote neuronal survival by phagocytosing dead cells and debris and by releasing neuroprotective and anti-inflammatory factors.However,excessive activation of microglia can lead to persistent inflammation and contribute to the formation of glial scars,which hinder axonal regeneration.Despite this,the precise role and mechanisms of microglia during the acute phase of spinal cord injury remain controversial and poorly understood.To elucidate the role of microglia in spinal cord injury,we employed the colony-stimulating factor 1 receptor inhibitor PLX5622 to deplete microglia.We observed that sustained depletion of microglia resulted in an expansion of the lesion area,downregulation of brain-derived neurotrophic factor,and impaired functional recovery after spinal cord injury.Next,we generated a transgenic mouse line with conditional overexpression of brain-derived neurotrophic factor specifically in microglia.We found that brain-derived neurotrophic factor overexpression in microglia increased angiogenesis and blood flow following spinal cord injury and facilitated the recovery of hindlimb motor function.Additionally,brain-derived neurotrophic factor overexpression in microglia reduced inflammation and neuronal apoptosis during the acute phase of spinal cord injury.Furthermore,through using specific transgenic mouse lines,TMEM119,and the colony-stimulating factor 1 receptor inhibitor PLX73086,we demonstrated that the neuroprotective effects were predominantly due to brain-derived neurotrophic factor overexpression in microglia rather than macrophages.In conclusion,our findings suggest the critical role of microglia in the formation of protective glial scars.Depleting microglia is detrimental to recovery of spinal cord injury,whereas targeting brain-derived neurotrophic factor overexpression in microglia represents a promising and novel therapeutic strategy to enhance motor function recovery in patients with spinal cord injury.
基金supported by National Natural Science Foundation of China[NO.11932013].
文摘This study aims to explore the impact of fatigue induced by different limb exercises on cerebral cortical oxygenation levels and functional connectivity strength using functional near-infrared spectroscopy(fNIRS).Fatigue was induced using an upper limb ergometer or a lower limb ergometer,with the load increasing gradually each minute.fNIRS covering the prefrontal cortex and motor cortex were used to collect data during the resting state,both before and after fatigue induction.A two-way ANOVA was conducted to examine differences in oxyhemoglobin(HbO_(2))and functional connectivity before and after fatigue induction in both groups,with the significance level set at 0.05.Exercise-induced fatigue in both the upper and lower limbs leads to a significant decrease in cerebral cortical oxygenation levels.Upper limb fatigue leads to a significant reduction in functional connectivity,there were significant decreases in connectivity within the motor cortex,between the motor cortex and frontal regions,and between the right ventrolateral prefrontal cortex and other frontal regions.Conversely,no significant changes were observed before and after lower limb fatigue.Future studies should focus on examining the extent to which how changes in the cerebral cortex,induced by exercise fatigue,are linked to exercise-and/or performance-related outcomes.
文摘In this paper,the isogeometric analysis(IGA)method is employed to analyze the oscillation characteristics of functionally graded triply periodic minimal surface(FG-TPMS)curved-doubly shells integrated with magneto-electric surface layers(referred to as"FG-TPMS-MEE curved-doubly shells")subjected to low-velocity impact loads.This study presents low-velocity impact load model based on a single springmass(S-M)approach.The FG-TPMS-MEE curved-doubly shells are covered with two magneto-electric surface layers,while the core layer consists of three types:I-graph and Wrapped Package-graph(IWP),Gyroid(G),and Primitive(P),with various graded functions.These types are notable for their exceptional stiffness-to-weight ratios,enabling a wide range of potential applications.The Maxwell equations and electromagnetic boundary conditions are applied to compute the change in electric potentials and magnetic potentials.The equilibrium equations of the shell are derived from a refined higher-order shear deformation theory(HSDT),and the transient responses of the FG-TPMS-MEE curveddoubly shells are subsequently determined using Newmark's direct integration method.These results have applications in structural vibration control and the analysis of structures subjected to impact or explosive loads.Furthermore,this study provides a theoretical prediction of the low-velocity impact load and magneto-electric-elastic effects on the free vibration and transient response of FG-TPMS-MEE curved-doubly shells.
基金Supported by The Natural Science Foundation of China,No.82374292the Plans for Major Provincial Science and Technology Projects of Anhui Province,No.202303a07020003the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine,No.ZYYCXTD-C-202401.
文摘Functional gastrointestinal disorders(FGIDs),including irritable bowel syndrome(IBS),functional dyspepsia(FD),and gastroesophageal reflux disease(GERD),present persistent diagnostic and therapeutic challenges due to symptom heterogeneity and the absence of reliable biomarkers.Artificial intelligence(AI)enables the integration of multimodal data to enhance FGID management through precision diagnostics and preventive healthcare.This minireview summarizes recent advancements in AI applications for FGIDs,highlighting progress in diagnostic accuracy,subtype classification,personalized interventions,and preventive strategies inspired by the traditional Chinese medicine concept of“treating the undiseased”.Machine learning and deep learning algorithms have demonstrated value in improving IBS diagnosis,refining FD neuro-gastrointestinal subtyping,and screening for GERD-related complications.Moreover,AI supports dietary,psychological,and integrative medicine-based interventions to improve patient adherence and quality of life.Nonetheless,key challenges remain,including data heterogeneity,limited model interpretability,and the need for robust clinical validation.Future directions emphasize interdisciplinary collaboration,the development of multimodal and explainable AI models,and the creation of patientcentered platforms to facilitate a shift from reactive treatment to proactive prevention.This review provides a systematic framework to guide the clinical application and theoretical innovation of AI in FGIDs.
基金supported by the National Natural Science Foundation of China(Grant Nos.52379107 and 52309141).
文摘The capillary pressure curve provides fundamental insights into the dynamics of fluid-fluid displacement and phase distributions.Capillary scaling is crucial for extrapolating capillary pressure-saturation data from laboratory tests to field applications.However,the classic scaling method fails to capture the effect of wettability as the pore surface approaches neutral wetting.Here,inspired by the role of pore-filling events in controlling fluid-fluid displacement,we perform a theoretical analysis of the burst events occurring during drainage processes.We find that the median threshold capillary pressure,which corresponds to the occurrence of burst events for the median pore throat,is closely correlated with the capillary pressure curve across various contact angles.Using this concept,we propose a new scaling method for capillary pressure curves under various wetting conditions.We conduct microfluidic experiments and pore-network modeling across different contact angles,porosities,and disorders to evaluate the new scaling methods,indicating that the new scaling method performs better than the Leverett J-function as the contact angle approaches 90°.We further perform geometry analysis on the critical radius of curvature for burst events in an ideal tetrahedral arrangement and extend the new scaling method to 3D(three-dimensional)porous media.Model evaluation shows that the 3D version of the scaling method also performs well but requires fewer parameters compared to the Leverett J-function.Our work enhances the prediction and interpretation of experimental data for capillary pressure curves under various wet conditions,and more importantly,establishes a methodology that relates Darcy-scale flow behavior to pore-scale fluid displacements.
基金supported by the key project of National Natural Sciences Foundation of China(U22A20551,32030085)the Major Project of Hubei Hongshan Laboratory,China(2021hszd015)+2 种基金the Hubei Province Major Science and Technology Special Project,China(2023BBA002)the National Natural Sciences Foundation of China(U22A20551)the National Natural Science Foundation of China Excellent Youth Fund(32422072)。
文摘Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural products,thereby endangering human health.Extensive studies on Aspergillus fungi have been conducted on growth and development,aflatoxin biosynthesis,and their interactions with environment.Here,we summarized a series of functional genes of the main Aspergillus fungi relative to toxins occurrence in foods,which revealed the signal transduction mechanisms of their involvement in growth and development,toxin production,and response to light,anticipating providing theoretical guidance on developing control and prevention technologies for mycotoxin contamination in agricultural products to ensure food safety.
基金support and funding by the European Union-Next Generation EU under the Italian Ministry of University and Research (MUR) National Innovation Ecosystem (No.ECS00000041-VITALITY and also “Ecosistema TECH4YOU-(Spoke 3-Goal 3.5)MUR is thanked for PRIN-PNRR 2022 project "P2022XKWH7-Circular Waste+3 种基金The University of Perugia is acknowledged for financial support to the university project “Fondo Ricerca di Ateneo,edizione 2022”The National Ph D program in Catalysis coordinated by the University of Perugia is also thankedthe financial supports of key research and development and technology transfer projects of Inner Mongolia Autonomous Region (No.2025KJHZ0008)major special projects of science and technology of Ordos (No.2022EEDSKJZDZX003)。
文摘Given the broad applicability of carbazole structural moieties in materials science and medicinal chemistry,significant efforts have been devoted to developing efficient synthetic catalytic methodologies to access this valuable scaffold.Catalyzed direct Csp^(2)-H functionalization provides an effective and costefficient approach to synthesizing carbazoles from simple and readily available starting materials,ensuring a promising path characterized by excellent atom and step economy.This review highlights the substantial progress made in the last 10 years in advancing catalytic Csp^(2)-H functionalization techniques for synthesizing carbazoles.
基金supported by the Fundamental Research Funds for the Central Universities(2042023gf0003)Hubei Provincial Natural Science Foundation of China(2024AFD126)National Key Research and Development Program of China(2023YFF1104404).
文摘Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear and contentious.This study offered a comprehensive review and meta-analysis of research published until June 2024,analyzing the effects of multivitamins on various cognitive functions in individuals aged 65 and older.We included ten randomized controlled trials encompassing 13,600 participants from multiple databases.These studies evaluated the impact of multivitamins on reasoning,memory,learning,visual perception,idea production,cognitive speed,psychomotor abilities,and higher cognitive functions.Our meta-analysis revealed that multivitamins significantly enhanced delayed free recall(SMD=0.09,95%confidence interval(CI)=[0.05,0.13],P=0.0001).However,they had no substantial effects on immediate free recall(SMD=0.85,95%CI=[-0.1,1.9],P=0.11),idea production(SMD=0.00,95%CI=[-0.04,0.03],P=0.86),or cognitive functioning(SMD=0.07,94%CI=[-0.07,0.14],P=0.006).Thus,while multivitamins facilitated delayed free recall,they did not significantly improve other cognitive functions in older adults.
文摘BACKGROUND An echocardiogram is an essential tool in the evaluation of potential kidney transplant recipients(KTRs).Despite cardiac clearance,potential KTRs still have structural and functional abnormalities.Identifying the prevalence of these abnormalities and understanding their predictors is vital for optimizing pretransplant risk stratification and improving post-transplant outcomes.AIM To determine the prevalence of left ventricular hypertrophy(LVH),left ventricular systolic dysfunction(LVSD),diastolic dysfunction(DD),pulmonary hypertension(PH),and their predictors,and to assess their impact on graft function in pre-transplant candidates.METHODS The study included all successful transplant candidates older than 14 who had a baseline echocardiogram.Binary logistic regression models were constructed to identify factors associated with LVH,LVSD,DD,and PH.RESULTS Out of 259 patients,LVH was present in 64%(166),12%(31)had LVSD,27.5%(71)had DD,and 66(25.5%)had PH.Independent predictors of LVH included male gender[odds ratio(OR):2.51;95%CI:1.17-5.41 P=0.02],PH(OR=2.07;95%CI:1.11-3.86;P=0.02),DD(OR:2.47;95%CI:1.29-4.73;P=0.006),and dyslipidemia(OR=1.94;95%CI:1.07-3.53;P=0.03).Predictors for LVSD included patients with DD(OR=3.3,95%CI:1.41-7.81;P=0.006)and a family history of coronary artery disease(OR=4.50,95%CI:1.33-15.20;P=0.015).Peritoneal dialysis was an independent predictor for DD(OR=10.03;95%CI:1.71-58.94,P=0.011).The presence of LVH(OR=3.32,95%CI:1.05-10.55,P=0.04)and mild to moderate or moderate to severe mitral regurgitation(OR=4.63,95%CI:1.45-14.78,P=0.01)were significant factors associated with PH.These abnormalities had no significant impact on estimated glomerular filtration at discharge,6 months,1 year,or 2 years post-transplant.CONCLUSION Significant echocardiographic abnormalities persist in a potential transplant candidate despite cardiac clearance,although they don’t affect future graft function.Understanding the risk factors associated with these abnormalities may help clinicians address these factors pre-and post-transplant to achieve better outcomes.
文摘The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic field.These microorganisms produce density gradients by swimming,which induces macroscopic convection flows in the fluid.This procedure improves the mass and heat transfer,illustrating the interaction between biological activity and fluid dynamics.Furthermore,instead of considering traditional Fourier's and Fick's law the energy and concentration equations are developed by incorporating Cattaneo-Christov double diffusion theory.Moreover,to examine the influence of thermophoresis and Brownian diffusions in the fluid we have adopted the Buongiorno nanofluid model.Due to the oscillation of the surface of the channel,the mathematical development of the considered flow problem is obtained in the form of partial differential equations via the curvilinear coordinate system.The convergent series solution of the governing flow equations is obtained after applying the homotopy analysis method(HAM).The effects of different pertinent flow parameters on velocity,motile microorganism density distribution,concentration,pressure,temperature,and skin friction coefficient are examined and discussed in detail with the help of graphs and tables.It is observed during the current study that the density of microorganisms is enhanced for higher values of Reynolds number,Peclet number,radius of curvature variable,and Lewis number.
基金Supported by Putuo District Science and Technology R&D Platform Project,Shanghai(2024QX04).
文摘[Objectives]To investigate the efficacy and potential mechanism of the topical preparation Jineijin-Shanzha Patch(composed of Galli Gigerii Endothelium Corneum and Crataegi Fructus)in improving functional dyspepsia(FD)based on network pharmacology.[Methods]Firstly,we reviewed the existing research progress on each constituent drug of the Jineijin Shanzha Patch for FD improvement.Following this,identified overlapping genes were utilized to construct both a"Drug-Active Component-FD Target"network and a Protein-Protein Interaction(PPI)network specific to the patch.In addition,Gene Ontology(GO)analysis was carried out.[Results]We identified that the 13 herbs in the Jineijin Shanzha Patch are mainly used for food stagnation,qi stagnation,and spleen deficiency.Screening revealed 43 active patch components,1414 FD-related targets,and 284 shared targets between them.The PPI network analysis further identified the top 10 core targets from these shared targets.From the"Drug-Active Component-FD Target"network,we identified the core elements.These included the herbal components Vignae Semen(from Liushenqu),Crataegi Fructus,and Pseudostellariae Radix;the active components quercetin,genistein,and apigenin;and the key targets CASP3,BCL2,and CASP9.GO analysis of the 284 overlapping targets indicated that the Jineijin Shanzha Patch may exert its therapeutic effects via regulation of biological processes such as the response to lipopolysaccharide,response to bacterium-derived molecules,and regulation of the apoptotic signaling pathway.[Conclusions]The main active components of the Jineijin Shanzha Patch(quercetin,genistein,and apigenin)may improve FD by modulating signaling pathways such as the response to lipopolysaccharide,response to bacterium-derived molecules,and regulation of the apoptotic signaling pathway,thereby acting on key targets including CASP3,BCL2,and CASP9.
基金National Natural Science Foundation of China,Grant/Award Number:82172140。
文摘Background:Targeted delivery of biological macromolecules to the small intestine remains challenging due to their susceptibility to degradation in the hostile gastric environment.Methods:This study introduces a minimally invasive,in situ injection technique for the murine small intestine that facilitates localized luminal delivery while circumventing gastric barriers.The procedure involves a small abdominal incision for direct injection into the duodenum near the pylorus.Postsurgical monitoring of physiological parameters,systemic inflammatory markers,liver function,and intestinal integrity was conducted over 72 h.Histopathological analysis was performed.The delivery of the functional protein TAT-EGFP(Tat protein fused to enhanced green fluorescent protein)to intestinal epithelial cells was evaluated and compared with oral gavage.As a proof of concept,single-cell RNA sequencing of the intestinal epithelium was performed after high-mobility group box 1 administration.Results:Postsurgical monitoring indicated only transient,anesthesia-related hypo-thermia and minor behavioral alterations.No significant changes were observed over 72 h in body weight,core temperature,clinical severity scores,systemic inflammatory markers(C-reactive protein and leukocytes),liver function(alanine aminotransferase),or intestinal integrity.Histopathological analysis confirmed preserved tissue architec-ture and normal digestive,absorptive,and barrier functions.The model successfully delivered TAT-EGFP to intestinal epithelial cells,an outcome not achievable via oral gavage due to gastric degradation.Single-cell RNA sequencing of the intestinal epi-thelium after high-mobility group box 1 administration revealed inflammatory gene expression patterns in specific epithelial subpopulations.Conclusions:Compared to traditional methods such as oral gavage or organoid cul-ture,this technique offers precise,degradation-resistant delivery of macromolecules in a physiological context.The model's versatility makes it a powerful platform for intestinal research,with applications in drug delivery assessment,gene therapy evalu-ation,and host-microbiota interaction studies.
文摘The original online version of this article was revised:The layout update for Article 758 has impacted the page range in the published issue,but did not affect the scholarly content.To ensure consistency with the originally assigned pages(2595-2614),we will need to publish an erratum to correct the article and restore the original page range.The original article has been corrected.
基金supported by the National Natural Science Foundation of China (No.21801111)the Training Plan for Young Core Teachers in Higher Education of Henan Province (No.2021GGJS131)+1 种基金Natural Science Foundation of Henan Province (No.232300421232)the Heluo Young Talent Lifting Project (No.2023HLTJ02)。
文摘Ln@MOFs by anchoring rare metal ions(Ln) into metal-organic frameworks(MOFs) are proved to have great potential in the field of luminescent molecular thermometer.Nevertheless,the current research indicated that the poor structural stability and low sensitivity hindered their application scope.In this work,a new MOF Zn-450 luminescent thermometer with multiple emission fluorescence characteristics was synthesized by the combination of 3,3,5,5-biphenyl tetracarboxylic acid(H_(4)L) and Zn^(2+) ion under solvothermal conditions.Interestingly,a high relative sensitivity of 1.43 % K^(-1) was found within 80-300 K based on Zn-450.Subsequently,two high-sensitivity luminescent Ln@MOFs(Ln = Eu and Tb) were further fabricated by doping rare earth ions into Zn-450 based on the post-synthesis strategy.Among them,the Eu@Zn-450 demonstrates various luminous behaviors while achieving an increased relative sensitivity of 1.63 % K^(-1).In addition,the continuously visible red,pink,and purple luminescent emissions at the same temperature range were observed,suggesting that the Eu@Zn-450 could be utilized as a luminescent colorimetric molecular thermometer.Importantly,this work can present new possibilities for the development of rare earth-doped luminescence and its temperature sensing properties.
基金Supported by Suzhou Clinical Medical Center for Mood Disorders,No.Szlcyxzx202109Suzhou Key Laboratory,No.SZS2024016Multicenter Clinical Research on Major Diseases in Suzhou,No.DZXYJ202413.
文摘BACKGROUND Suicide constitutes the second leading cause of death among adolescents globally and represents a critical public health concern.The neural mechanisms underlying suicidal behavior in adolescents with major depressive disorder(MDD)remain poorly understood.Aberrant resting-state functional connectivity(rsFC)in the amygdala,a key region implicated in emotional regulation and threat detection,is strongly implicated in depression and suicidal behavior.AIM To investigate rsFC alterations between amygdala subregions and whole-brain networks in adolescent patients with depression and suicide attempts.METHODS Resting-state functional magnetic resonance imaging data were acquired from 32 adolescents with MDD and suicide attempts(sMDD)group,33 adolescents with MDD but without suicide attempts(nsMDD)group,and 34 demographically matched healthy control(HC)group,with the lateral and medial amygdala(MeA)defined as regions of interest.The rsFC patterns of amygdala subregions were compared across the three groups,and associations between aberrant rsFC values and clinical symptom severity scores were examined.RESULTS Compared with the nsMDD group,the sMDD group exhibited reduced rsFC between the right lateral amygdala(LA)and the right inferior occipital gyrus as well as the left middle occipital gyrus.Compared with the HC group,the abnormal brain regions of rsFC in the sMDD group and nsMDD group involve the parahippocampal gyrus(PHG)and fusiform gyrus.In the sMDD group,right MeA and right temporal pole:Superior temporal gyrus rsFC value negatively correlated with the Rosenberg Self-Esteem Scale scores(r=-0.409,P=0.025),while left LA and right PHG rsFC value positively correlated with the Adolescent Self-Rating Life Events Checklist interpersonal relationship scores(r=0.372,P=0.043).CONCLUSION Aberrant rsFC changes between amygdala subregions and these brain regions provide novel insights into the underlying neural mechanisms of suicide attempts in adolescents with MDD.
