The inhibitory effect of gadolinium on Sinorhizobium fredii USDA 205 was studied on a global scale using twodimensional gel electrophoresis and MALDI-TOF MS. The results indicated that 22 proteins were significantly a...The inhibitory effect of gadolinium on Sinorhizobium fredii USDA 205 was studied on a global scale using twodimensional gel electrophoresis and MALDI-TOF MS. The results indicated that 22 proteins were significantly affected by 1 mmol · L^-1 Gd^3 ＋ treatment when compared with an untreated control. Among these proteins, nine were up-regulated and thirteen were down-regulated. The differently expressed proteins were classified into 8 functional categories based on their functions, including transporters, proteins for cellular defence, and proteins involved in metabolism.展开更多
The effect of pH on the nodulation of Sinorhizobium fredii and Bradyrhizobium japonicum was examined by analy- zing the indigent soybean rhizobia,predominant indigent rhizobia,and specific rhizobia,respectively.The re...The effect of pH on the nodulation of Sinorhizobium fredii and Bradyrhizobium japonicum was examined by analy- zing the indigent soybean rhizobia,predominant indigent rhizobia,and specific rhizobia,respectively.The results showed that very acid and very alkaline environment could retard the nodulation and inhibit the growth of the rhizobia.Sinorhizohium fredii could endure environment more strongly than Bradyrhizobium japonicum,and had a high competitive nodulation capacity.Bradyhizobium japonicum could endure acid environment more strongly than Sinorhizobium fredii.In very acid and very alkaline environment,the nodulation capacity of S.fredii and B.japonicum was mainly determined by their physiological characteristics.展开更多
为了进一步研究发掘新的根瘤菌资源库,采用平板划线分离的方法从大豆根瘤中分离纯化根瘤菌,并且将获得的菌株进行盆栽回接试验,结果表明,分离获得的大豆根瘤菌菌株均可在大豆和苜蓿上结瘤,具有结瘤能力。根据已公布大豆根瘤菌共同结瘤基...为了进一步研究发掘新的根瘤菌资源库,采用平板划线分离的方法从大豆根瘤中分离纯化根瘤菌,并且将获得的菌株进行盆栽回接试验,结果表明,分离获得的大豆根瘤菌菌株均可在大豆和苜蓿上结瘤,具有结瘤能力。根据已公布大豆根瘤菌共同结瘤基因nod A、nif H、16S r DNA保守区域设计引物,并且对nod A、nif H、16S r DNA序列进行扩增分析和系统发育分析,由此鉴定分离的根瘤菌为费式中华根瘤菌,从而在分子水平上实现了对大豆根瘤菌的快速鉴定。展开更多
基金Project supported by the National Natural Science Foundation of China (G2001CB108902 ,2004CB418506)
文摘The inhibitory effect of gadolinium on Sinorhizobium fredii USDA 205 was studied on a global scale using twodimensional gel electrophoresis and MALDI-TOF MS. The results indicated that 22 proteins were significantly affected by 1 mmol · L^-1 Gd^3 ＋ treatment when compared with an untreated control. Among these proteins, nine were up-regulated and thirteen were down-regulated. The differently expressed proteins were classified into 8 functional categories based on their functions, including transporters, proteins for cellular defence, and proteins involved in metabolism.
基金ACKN0WLEDGEMENT This work is part of the joint EC-prokect no. TS3-CT92-0127 and was supported in part by the National Natural Science Foundation of China （NSFC）.
文摘The effect of pH on the nodulation of Sinorhizobium fredii and Bradyrhizobium japonicum was examined by analy- zing the indigent soybean rhizobia,predominant indigent rhizobia,and specific rhizobia,respectively.The results showed that very acid and very alkaline environment could retard the nodulation and inhibit the growth of the rhizobia.Sinorhizohium fredii could endure environment more strongly than Bradyrhizobium japonicum,and had a high competitive nodulation capacity.Bradyhizobium japonicum could endure acid environment more strongly than Sinorhizobium fredii.In very acid and very alkaline environment,the nodulation capacity of S.fredii and B.japonicum was mainly determined by their physiological characteristics.
文摘为了进一步研究发掘新的根瘤菌资源库,采用平板划线分离的方法从大豆根瘤中分离纯化根瘤菌,并且将获得的菌株进行盆栽回接试验,结果表明,分离获得的大豆根瘤菌菌株均可在大豆和苜蓿上结瘤,具有结瘤能力。根据已公布大豆根瘤菌共同结瘤基因nod A、nif H、16S r DNA保守区域设计引物,并且对nod A、nif H、16S r DNA序列进行扩增分析和系统发育分析,由此鉴定分离的根瘤菌为费式中华根瘤菌,从而在分子水平上实现了对大豆根瘤菌的快速鉴定。
