FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex...FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex determination and gonadal development in Cyprinus carpio var.koi were explored using a non-invasive RNA interference(RNAi)method,histopathological observation and qPCR.Results demonstrate that foxl 2 exhibited a sexually dimorphic expression pattern in gonads,with a notable expression in ovaries;cyp 19 b was expressed in all peripheral tissues,with a particularly prominent expression in brain and gonads.The knockdown of foxl 2 by RNAi resulted in delay in the development of the female gonads.Conversely,no notable alterations were discerned in the gonads of C.carpio var.koi following the knockdown of cyp 19 b.The upregulation of sox 9 a,amh,and cyp 19 b following foxl 2 knockdown indicates that foxl 2 may play a pivotal role in gonadal development.Nevertheless,further investigation is required to ascertain the potential role of cyp 19 b.This study elucidated the role of foxl 2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C.carpio var.koi.展开更多
Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes.The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system,where most males initially ...Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes.The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system,where most males initially develop juvenile ovaries before committing to male fate.In species with chromosomal sex determination,gonadal somatic cells are recognized as key determinants of germ cell fate.Notably,the loss of germ cells in zebrafish leads to masculinization,implying that germ cells harbor an intrinsic feminization signal.However,the specific signal triggering oogenesis in zebrafish remains unclear.In the present study,we identified foxl2l as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells.Results showed that foxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males.Further analysis revealed that loss of foxl2l hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis,leading to premature spermatogenesis during early gonadal development.Furthermore,while mutation of the pro-male gene dmrt1 led to fertile female differentiation,simultaneous disruption of foxl2l in dmrt1 mutants completely blocked oogenesis,with a large proportion of germ cells arrested as germline stem cells,highlighting the crucial role of foxl2l in oogenesis.Overall,this study highlights the unique function of foxl2l as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.展开更多
基金Supported by the Qingdao Aquarium Technology Collaborative Innovation Center Cooperation Project(No.20210021)the Researching Key Technologies for Selecting Excellent Koi Carp Germplasm(No.20223702032291)the Qingdao Agricultural University Tangwang Koi Carp Joint R&D Center Collaborative Project(No.20220271)。
文摘FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex determination and gonadal development in Cyprinus carpio var.koi were explored using a non-invasive RNA interference(RNAi)method,histopathological observation and qPCR.Results demonstrate that foxl 2 exhibited a sexually dimorphic expression pattern in gonads,with a notable expression in ovaries;cyp 19 b was expressed in all peripheral tissues,with a particularly prominent expression in brain and gonads.The knockdown of foxl 2 by RNAi resulted in delay in the development of the female gonads.Conversely,no notable alterations were discerned in the gonads of C.carpio var.koi following the knockdown of cyp 19 b.The upregulation of sox 9 a,amh,and cyp 19 b following foxl 2 knockdown indicates that foxl 2 may play a pivotal role in gonadal development.Nevertheless,further investigation is required to ascertain the potential role of cyp 19 b.This study elucidated the role of foxl 2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C.carpio var.koi.
基金supported by the National Natural Science Foundation of China(32025037)Ministry of Science and Technology of China(2023YFD2401603)+2 种基金Ministry of Agriculture and Rural Affairs of China(NK2022010207)Science and Technology Special Fund of Hainan Province(ZDYF2024XDNY256)Natural Science Foundation of Wuhan。
文摘Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes.The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system,where most males initially develop juvenile ovaries before committing to male fate.In species with chromosomal sex determination,gonadal somatic cells are recognized as key determinants of germ cell fate.Notably,the loss of germ cells in zebrafish leads to masculinization,implying that germ cells harbor an intrinsic feminization signal.However,the specific signal triggering oogenesis in zebrafish remains unclear.In the present study,we identified foxl2l as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells.Results showed that foxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males.Further analysis revealed that loss of foxl2l hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis,leading to premature spermatogenesis during early gonadal development.Furthermore,while mutation of the pro-male gene dmrt1 led to fertile female differentiation,simultaneous disruption of foxl2l in dmrt1 mutants completely blocked oogenesis,with a large proportion of germ cells arrested as germline stem cells,highlighting the crucial role of foxl2l in oogenesis.Overall,this study highlights the unique function of foxl2l as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.
