Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCU...Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCUS T） gene was involved in the photoperiod pathway to regulate fowering in the model plant. In this study, transcriptome sequencing method was used to obtain cDNA sequence of FT homologous gene in onion, named AcFT3 （KF864665）. AcFT3 had a full-length of 540 bp, encoded 179 amino acids, with 98.31% homology to AfFT （Allium fstulosum）, and 63.0%-84.0% homology to other higher plants. Phylogenetic tree analysis indicated that AcFT3 had the closest relationship with AfFT. The results of quantitative RT-PCR showed the expression pattern of AcFT3 both in vegetative growth of onion and in different organs of bolting and fowering, the expression level of AcFT3 reached the highest in the leaves before bolting and in the fower organs after bolting.展开更多
A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by...A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by Ministry of Agriculture of China The results showed that five characteristics such as the surface colour, the back color of ray floret among them were siginificantly different, their MS （mo by AFLP showed that rphological s GS （genetic imilarity） was 91.4%, and MD （morphological difference） was 8.62%. DNA polymorphic analysis similarity） was 98.6%, and GD （genetic polymorphic diversity） was 2.81%. Five distinct bands which may include the flower-colour sport genes or be the molecular marker linkaged flower-color characteristics were amplified.展开更多
Although the effects of arbuscular mycorrhizal fungi(AMF)on host plants have been well documented,whether the effects of AMF on parental generations affect offspring performance is not fully clear.We conducted a commo...Although the effects of arbuscular mycorrhizal fungi(AMF)on host plants have been well documented,whether the effects of AMF on parental generations affect offspring performance is not fully clear.We conducted a common garden experiment to determine whether AMF status of host plants(Medicago truncatula)affects phenotype and transcriptome expression of their offspring.Seeds from four type parental treatments(low-phosphorus(P)soil without AMF,low-P soil with AMF,high-P soil without AMF and high-P soil with AMF)were grown under low-P(LPS)and normal-P soil(OHS)conditions.The fowering pattern of LP offspring was similar to their parents,such that plants with AMF fowered earlier than those without AMF under OHS condition but were opposite under LPS condition.The transcriptome differential analysis showed that some differential transcripts(45 for parental plants growing under low-P condition and 3 for parental plants growing under high-P condition)expression patterns between offspring were comparable,and that only affected by parental AMF status regardless of the P environment that offspring was grown.Others(146 for parental plants growing under low-P condition and 2 for parental plants growing under high-P condition),however,were affected both by the parental AMF status and the offspring P environment.In addition,the number of differential transcripts between offspring whose parental plants grew under high-P condition was far less than under low-P condition.These results indicate that AMF may not only affect the current generation of host plants but also affect the offspring especially when their parents have experienced a stressful environment.展开更多
Burans is an evergreen tree that bears fowers with colors ranging from bright red to pink and are distributed throughout the world.The fowers of Burans exhibit many therapeutic,nutritional,and aromatic properties alon...Burans is an evergreen tree that bears fowers with colors ranging from bright red to pink and are distributed throughout the world.The fowers of Burans exhibit many therapeutic,nutritional,and aromatic properties along with diverse health benefts.These characteristics of Burans can have a great impact on the quality of alcoholic wines and provide value addition to the Burans fowers as a fermented product,which is otherwise wasted.Thus,in this study,Burans fowers were used for the preparation of a new favor of the wine to enhance the utility of this fower.Fermentation time(5,8 and 11 days),temperature(30,32 and 34℃),pH(3.5,4.0 and 4.50),and total soluble solids(TSS:22,24 and 26°Bx)were varied to identify the most suitable conditions for high-quality wine production.The titratable acidity,reducing sugar,pH,and TSS of Burans wine were analyzed after fermentation which ranged from 0.36 to 0.78%,9.8 to 13.3%,3.43 to 4.13,and 6.9 to 16.8ºBx,respectively.This study also provides a useful benchmark for the industrial production of Burans fower wine with a standard alcohol content of 14.1%.展开更多
基金Supported by Fund Project of Heilongjiang Province(C2015017)Heilongjiang Provincial Science and Technology Commissioner Project(GC13B09)
文摘Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCUS T） gene was involved in the photoperiod pathway to regulate fowering in the model plant. In this study, transcriptome sequencing method was used to obtain cDNA sequence of FT homologous gene in onion, named AcFT3 （KF864665）. AcFT3 had a full-length of 540 bp, encoded 179 amino acids, with 98.31% homology to AfFT （Allium fstulosum）, and 63.0%-84.0% homology to other higher plants. Phylogenetic tree analysis indicated that AcFT3 had the closest relationship with AfFT. The results of quantitative RT-PCR showed the expression pattern of AcFT3 both in vegetative growth of onion and in different organs of bolting and fowering, the expression level of AcFT3 reached the highest in the leaves before bolting and in the fower organs after bolting.
文摘A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by Ministry of Agriculture of China The results showed that five characteristics such as the surface colour, the back color of ray floret among them were siginificantly different, their MS （mo by AFLP showed that rphological s GS （genetic imilarity） was 91.4%, and MD （morphological difference） was 8.62%. DNA polymorphic analysis similarity） was 98.6%, and GD （genetic polymorphic diversity） was 2.81%. Five distinct bands which may include the flower-colour sport genes or be the molecular marker linkaged flower-color characteristics were amplified.
基金supported by the National Natural Science Foundation of China(31470483,31570411)the Postdoctoral Science Foundation of China(2021M693732)the Postdoctoral Science Foundation of Chongqing,China(cstc2021jcyj-bshX0165)。
文摘Although the effects of arbuscular mycorrhizal fungi(AMF)on host plants have been well documented,whether the effects of AMF on parental generations affect offspring performance is not fully clear.We conducted a common garden experiment to determine whether AMF status of host plants(Medicago truncatula)affects phenotype and transcriptome expression of their offspring.Seeds from four type parental treatments(low-phosphorus(P)soil without AMF,low-P soil with AMF,high-P soil without AMF and high-P soil with AMF)were grown under low-P(LPS)and normal-P soil(OHS)conditions.The fowering pattern of LP offspring was similar to their parents,such that plants with AMF fowered earlier than those without AMF under OHS condition but were opposite under LPS condition.The transcriptome differential analysis showed that some differential transcripts(45 for parental plants growing under low-P condition and 3 for parental plants growing under high-P condition)expression patterns between offspring were comparable,and that only affected by parental AMF status regardless of the P environment that offspring was grown.Others(146 for parental plants growing under low-P condition and 2 for parental plants growing under high-P condition),however,were affected both by the parental AMF status and the offspring P environment.In addition,the number of differential transcripts between offspring whose parental plants grew under high-P condition was far less than under low-P condition.These results indicate that AMF may not only affect the current generation of host plants but also affect the offspring especially when their parents have experienced a stressful environment.
文摘Burans is an evergreen tree that bears fowers with colors ranging from bright red to pink and are distributed throughout the world.The fowers of Burans exhibit many therapeutic,nutritional,and aromatic properties along with diverse health benefts.These characteristics of Burans can have a great impact on the quality of alcoholic wines and provide value addition to the Burans fowers as a fermented product,which is otherwise wasted.Thus,in this study,Burans fowers were used for the preparation of a new favor of the wine to enhance the utility of this fower.Fermentation time(5,8 and 11 days),temperature(30,32 and 34℃),pH(3.5,4.0 and 4.50),and total soluble solids(TSS:22,24 and 26°Bx)were varied to identify the most suitable conditions for high-quality wine production.The titratable acidity,reducing sugar,pH,and TSS of Burans wine were analyzed after fermentation which ranged from 0.36 to 0.78%,9.8 to 13.3%,3.43 to 4.13,and 6.9 to 16.8ºBx,respectively.This study also provides a useful benchmark for the industrial production of Burans fower wine with a standard alcohol content of 14.1%.
