[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods...[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods]The chromatographic separation was performed on a GL Sciences(19H0044724)-C_(18)column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.05%formic acid solution at a flow rate of 1 mL/min.The injection volume was 10μL,detection wavelength was set at 280 nm,and column temperature was maintained at 25℃.[Results]The linear ranges of forsythin,baicalin,and chlorogenic acid were 10.5-52.5,20.6-103,and 14.2-71μg/mL,respectively,with good linear relationships between concentration and peak area(R^(2)=0.9999).The relative standard deviations(RSD s)for precision and repeatability tests were all≤1.0%.The average recoveries were 98.51%,98.48%,and 97.71%for the three components,with RSD s of 0.96%,0.97%,and 0.73%,respectively.[Conclusions]This method demonstrates strong specificity,high precision,excellent accuracy,and simplicity of operation,making it suitable for the simultaneous quantification of forsythin,baicalin,and chlorogenic acid in traditional Chinese medicine spray disinfectants.It provides a reliable basis for quality control and practical applications in animal breeding environments.展开更多
Forsythia suspensa,belonging to the deciduous shrubs of the Luteaceae family,a traditional Chinese medicine,has effects of alleviating swelling,clearing heat,detoxification and promoting blood circulation.The leaves o...Forsythia suspensa,belonging to the deciduous shrubs of the Luteaceae family,a traditional Chinese medicine,has effects of alleviating swelling,clearing heat,detoxification and promoting blood circulation.The leaves of F.suspensa contain multiple chemical components and have a long history of use in folk medicines and health foods.The purpose of this study was to explore the effects of forsythin extract from F.suspensa leaves on intestinal microbiota and short-chain fatty acid(SCFA)content in rats with obesity induced by a high-fat diet.Forsythin extract in F.suspensa leaves increased the abundance of the intestinal microbiota,ameliorated intestinal microbiota disorders and inhibited the increase in total SCFA content in the intestinal tract in rats with obesity induced by a high-fat diet.These results suggested that forsythin extract in F.suspensa leaves may slow the development of obesity induced by a high-fat diet;thus,its active components and efficacy are worthy of further study.展开更多
Objective: To access the toxicity of forsythin from Forsythia suspensa leaves and evaluate its safety,Methods: Acute toxicity was determined by oral administration of a single dose of 18 100 mg/kg forsythin in NIH mic...Objective: To access the toxicity of forsythin from Forsythia suspensa leaves and evaluate its safety,Methods: Acute toxicity was determined by oral administration of a single dose of 18 100 mg/kg forsythin in NIH mice,Sub-chronic toxicity was evaluated by oral administration of several doses of forsythin for 30 days at does of 0,540,1 620,and 6 480 mg/kg in SD rats.Results: In the acute toxicity study,mortality was not observed after 14 days,In addition,clinically relevant adverse effects,or variations in body weight or food consumption were not observed,Similarly,after 30 days in the sub-chronic toxicity study,no mortality or significant toxicological effects such as decreased food consumption,body weight,biochemical parameters and vital organs etc,were noticed,Conclusion: The results revealed that the forsythin from Forsythia suspensa leaves has low or no toxicity via oral administration,and therefore is suitable for further development and applications.展开更多
Weeping forsythia (Forsythia suspensa,Oleaceae) is a deciduous broad-leaved tree species distributed in the warm temperate zone of China.However,the species still lacks a chromosome-level genome.In this study,the form...Weeping forsythia (Forsythia suspensa,Oleaceae) is a deciduous broad-leaved tree species distributed in the warm temperate zone of China.However,the species still lacks a chromosome-level genome.In this study,the former draft genome (Accession No.WIPI00000000) of weeping forsythia was assembled into 14 chromosomes with a 712.9 Mb genome size.Weeping forsythia underwent a and b whole-genome duplication events.After the divergence between weeping forsythia and Olea europaea,1 453 gene families had a significant expansion,and 1 146 gene families had a significant contraction.The enrichment pathways and ontologies of expanded genes suggested that the tillering,photosynthesis and growth capacity of weeping forsythia were enhanced after the divergence of weeping forsythia and O.europaea.The contracted genes suggested that the resistance of weeping forsythia to cold and drought was weakened.The last glacial period led to a significant decline in the effective population size of weeping forsythia.Forty-six candidate genes were identified for the synthesis of the forsythin and forsythoside A by genomic and transcriptomic data.In this study,we improved the previous draft genome of weeping forsythia.Our genome will provide genomic resources for the subsequent evolution and breeding research of weeping forsythia.展开更多
[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitati...[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.展开更多
目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质...目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质量控制与评价提供方法及参考依据。方法HPLC法分析QHY中黄芩苷、连翘酯苷A、熊果苷、五福花苷酸、连翘酯苷I、连翘苷、汉黄芩苷、黄芩素、汉黄芩素、水苏糖、棉子糖11种成分含量,对9个厂家的177批次QHY样品进行定量测定;利用SIMCA 14.1和SPSS 25.0软件,对上述11种成分含量检测结果进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA),筛选造成QHY质量差异的特征标志物;在PCA基础上设定Hotlling’s T2和DMod X控制限,对177批间样品进行质量差异的监控;运用熵权-TOPSIS模型对9个厂家QHY质量的优劣排序,多角度建立QHY质量综合评价方法。结果11种成分含量测定方法学考察结果精密度、重复性、稳定性及准确度的RSD值均小于3.0%。HCA和PCA结果均显示,不同厂家间及厂家内部不同批次间样品质量均有差异。OPLS-DA模型以VIP值>1为标准,筛选出水苏糖、棉子糖、汉黄芩素、黄芩苷、连翘酯苷I、连翘酯苷A 6个特征标志物,可能是影响QHY质量的主要成分。熵权-TOPSIS法结果显示,相对接近度C在0.084~0.850,表明不同厂家间QHY质量差异较大,厂家TCQ排序最优,厂家JM排序最后。结论建立的QHY中11种化学成分含量测定方法准确可靠,HCA、PCA和OPLS-DA可有效筛选影响QHY质量的特征标志物,熵权-TOPSIS法可综合评价QHY质量。展开更多
基金Supported by Shandong Province Major Agricultural Technologies Collaborative Promotion Project(SDNYXTTG-2024-04)National Key Laboratory for Quality Control of Chinese Medicinal Materials and Decoction Pieces Project(2024GSMPA-KL16)Weifang Municipal Science and Technology Bureau Project(2021GX031).
文摘[Objectives]To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of forsythin,baicalin,and chlorogenic acid in a traditional Chinese medicine spray disinfectant.[Methods]The chromatographic separation was performed on a GL Sciences(19H0044724)-C_(18)column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.05%formic acid solution at a flow rate of 1 mL/min.The injection volume was 10μL,detection wavelength was set at 280 nm,and column temperature was maintained at 25℃.[Results]The linear ranges of forsythin,baicalin,and chlorogenic acid were 10.5-52.5,20.6-103,and 14.2-71μg/mL,respectively,with good linear relationships between concentration and peak area(R^(2)=0.9999).The relative standard deviations(RSD s)for precision and repeatability tests were all≤1.0%.The average recoveries were 98.51%,98.48%,and 97.71%for the three components,with RSD s of 0.96%,0.97%,and 0.73%,respectively.[Conclusions]This method demonstrates strong specificity,high precision,excellent accuracy,and simplicity of operation,making it suitable for the simultaneous quantification of forsythin,baicalin,and chlorogenic acid in traditional Chinese medicine spray disinfectants.It provides a reliable basis for quality control and practical applications in animal breeding environments.
基金funded by grants from the National Key R&D Program of China(2016YFD0500604)。
文摘Forsythia suspensa,belonging to the deciduous shrubs of the Luteaceae family,a traditional Chinese medicine,has effects of alleviating swelling,clearing heat,detoxification and promoting blood circulation.The leaves of F.suspensa contain multiple chemical components and have a long history of use in folk medicines and health foods.The purpose of this study was to explore the effects of forsythin extract from F.suspensa leaves on intestinal microbiota and short-chain fatty acid(SCFA)content in rats with obesity induced by a high-fat diet.Forsythin extract in F.suspensa leaves increased the abundance of the intestinal microbiota,ameliorated intestinal microbiota disorders and inhibited the increase in total SCFA content in the intestinal tract in rats with obesity induced by a high-fat diet.These results suggested that forsythin extract in F.suspensa leaves may slow the development of obesity induced by a high-fat diet;thus,its active components and efficacy are worthy of further study.
基金supported by the Basic Science Research Program of Guangdong Province Science and Technology Plan Projects Fund[grant numbers:2016A010119136]the High-level Leading Talent Introduction Program of GDAS[grant numbers:2016GDASRC-0104]
文摘Objective: To access the toxicity of forsythin from Forsythia suspensa leaves and evaluate its safety,Methods: Acute toxicity was determined by oral administration of a single dose of 18 100 mg/kg forsythin in NIH mice,Sub-chronic toxicity was evaluated by oral administration of several doses of forsythin for 30 days at does of 0,540,1 620,and 6 480 mg/kg in SD rats.Results: In the acute toxicity study,mortality was not observed after 14 days,In addition,clinically relevant adverse effects,or variations in body weight or food consumption were not observed,Similarly,after 30 days in the sub-chronic toxicity study,no mortality or significant toxicological effects such as decreased food consumption,body weight,biochemical parameters and vital organs etc,were noticed,Conclusion: The results revealed that the forsythin from Forsythia suspensa leaves has low or no toxicity via oral administration,and therefore is suitable for further development and applications.
基金supported by the Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry)(Grant No.TGB2021004)National Natural Science Foundation of China (Grant Nos.31770225,31570594)Program of Guangzhou Municipal Science and Technology Bureau(Grant No.202102021257)。
文摘Weeping forsythia (Forsythia suspensa,Oleaceae) is a deciduous broad-leaved tree species distributed in the warm temperate zone of China.However,the species still lacks a chromosome-level genome.In this study,the former draft genome (Accession No.WIPI00000000) of weeping forsythia was assembled into 14 chromosomes with a 712.9 Mb genome size.Weeping forsythia underwent a and b whole-genome duplication events.After the divergence between weeping forsythia and Olea europaea,1 453 gene families had a significant expansion,and 1 146 gene families had a significant contraction.The enrichment pathways and ontologies of expanded genes suggested that the tillering,photosynthesis and growth capacity of weeping forsythia were enhanced after the divergence of weeping forsythia and O.europaea.The contracted genes suggested that the resistance of weeping forsythia to cold and drought was weakened.The last glacial period led to a significant decline in the effective population size of weeping forsythia.Forty-six candidate genes were identified for the synthesis of the forsythin and forsythoside A by genomic and transcriptomic data.In this study,we improved the previous draft genome of weeping forsythia.Our genome will provide genomic resources for the subsequent evolution and breeding research of weeping forsythia.
文摘[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.
文摘目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质量控制与评价提供方法及参考依据。方法HPLC法分析QHY中黄芩苷、连翘酯苷A、熊果苷、五福花苷酸、连翘酯苷I、连翘苷、汉黄芩苷、黄芩素、汉黄芩素、水苏糖、棉子糖11种成分含量,对9个厂家的177批次QHY样品进行定量测定;利用SIMCA 14.1和SPSS 25.0软件,对上述11种成分含量检测结果进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA),筛选造成QHY质量差异的特征标志物;在PCA基础上设定Hotlling’s T2和DMod X控制限,对177批间样品进行质量差异的监控;运用熵权-TOPSIS模型对9个厂家QHY质量的优劣排序,多角度建立QHY质量综合评价方法。结果11种成分含量测定方法学考察结果精密度、重复性、稳定性及准确度的RSD值均小于3.0%。HCA和PCA结果均显示,不同厂家间及厂家内部不同批次间样品质量均有差异。OPLS-DA模型以VIP值>1为标准,筛选出水苏糖、棉子糖、汉黄芩素、黄芩苷、连翘酯苷I、连翘酯苷A 6个特征标志物,可能是影响QHY质量的主要成分。熵权-TOPSIS法结果显示,相对接近度C在0.084~0.850,表明不同厂家间QHY质量差异较大,厂家TCQ排序最优,厂家JM排序最后。结论建立的QHY中11种化学成分含量测定方法准确可靠,HCA、PCA和OPLS-DA可有效筛选影响QHY质量的特征标志物,熵权-TOPSIS法可综合评价QHY质量。
