目的:探讨微小RNA-122(miR-122)在小鼠急性缺血性脑卒中的影响及其作用机制。方法:80只健康雄性C57BL/6小鼠随机分为4组,假手术组(sham)、大脑中动脉阻塞组(MCAO)、miR-122模拟物注射组(MACO+miR-122 mimics)和miR-122抑制物注射组(MACO...目的:探讨微小RNA-122(miR-122)在小鼠急性缺血性脑卒中的影响及其作用机制。方法:80只健康雄性C57BL/6小鼠随机分为4组,假手术组(sham)、大脑中动脉阻塞组(MCAO)、miR-122模拟物注射组(MACO+miR-122 mimics)和miR-122抑制物注射组(MACO+miR-122 inhibitor)。电凝法建立永久性局灶性小鼠MCAO模型,脑室注射miR-122模拟物、miR-122抑制物后采用Longa神经功能评分评估小鼠神经功能缺损程度;real time RT-PCR检测各组小鼠脑组织NOD样受体蛋白家族炎症小体3(NLRP3)的受体蛋白NLRP3、白介素1β(IL-1β)mRNA的表达差异;2,3,5-氯化三苯基四氮唑(TTC)染色评估小鼠脑梗死体积;酶联免疫吸附测定(ELISA)检测小鼠血清NLRP3、IL-1β蛋白表达。结果:Longa神经功能评分结果显示脑室注射miR-122模拟物后可显著改善小鼠神经功能评分,差异有统计学意义(P<0.05)。与sham组相比MCAO组脑组织NLRP3、IL1βmRNA表达显著升高;real time RT-PCR、ELISA的结果显示脑室注射miR-122模拟物后NLRP3和IL-1β表达量降低,差异均有统计学意义(P<0.05),脑室注射miR-122抑制物后NLRP3和IL-1β表达量增高,差异均有统计学意义(P<0.05);TTC染色结果显示脑室注射miR-122模拟物可减小小鼠脑梗死体积,差异有统计学意义(P<0.05)。结论:MCAO模型小鼠中miR-122通过下调炎症因子NLRP3和IL-1β的表达发挥神经保护作用,从而减轻小鼠脑梗死体积。展开更多
Hills Like White Elephants is written by Ernest Hemingway,who is one of the greatest representatives of the American literature.Ingenious symbolism and efficient and powerful dialogue combine to make the story a repre...Hills Like White Elephants is written by Ernest Hemingway,who is one of the greatest representatives of the American literature.Ingenious symbolism and efficient and powerful dialogue combine to make the story a representative of Hemingway's short stories.What is especially successful and notable about this book is that it fully represents the author’s style of the"iceberg",where space of multi-profound meanings is hidden in the simple plot.It is so impressive because Ernest Hemingway has succeeded in presenting the deep meaning as close as possible to the readers through the simple and concise words.This paper tries to carry out a research on the narrative rhetoric of Hills Like White Elephants from three aspects.The first part begins with the analysis of its narrator and narrative situation.The second part centers on the focalization.In the last part,the emphasis is put on the narrative modes.展开更多
文摘目的:探讨微小RNA-122(miR-122)在小鼠急性缺血性脑卒中的影响及其作用机制。方法:80只健康雄性C57BL/6小鼠随机分为4组,假手术组(sham)、大脑中动脉阻塞组(MCAO)、miR-122模拟物注射组(MACO+miR-122 mimics)和miR-122抑制物注射组(MACO+miR-122 inhibitor)。电凝法建立永久性局灶性小鼠MCAO模型,脑室注射miR-122模拟物、miR-122抑制物后采用Longa神经功能评分评估小鼠神经功能缺损程度;real time RT-PCR检测各组小鼠脑组织NOD样受体蛋白家族炎症小体3(NLRP3)的受体蛋白NLRP3、白介素1β(IL-1β)mRNA的表达差异;2,3,5-氯化三苯基四氮唑(TTC)染色评估小鼠脑梗死体积;酶联免疫吸附测定(ELISA)检测小鼠血清NLRP3、IL-1β蛋白表达。结果:Longa神经功能评分结果显示脑室注射miR-122模拟物后可显著改善小鼠神经功能评分,差异有统计学意义(P<0.05)。与sham组相比MCAO组脑组织NLRP3、IL1βmRNA表达显著升高;real time RT-PCR、ELISA的结果显示脑室注射miR-122模拟物后NLRP3和IL-1β表达量降低,差异均有统计学意义(P<0.05),脑室注射miR-122抑制物后NLRP3和IL-1β表达量增高,差异均有统计学意义(P<0.05);TTC染色结果显示脑室注射miR-122模拟物可减小小鼠脑梗死体积,差异有统计学意义(P<0.05)。结论:MCAO模型小鼠中miR-122通过下调炎症因子NLRP3和IL-1β的表达发挥神经保护作用,从而减轻小鼠脑梗死体积。
文摘Hills Like White Elephants is written by Ernest Hemingway,who is one of the greatest representatives of the American literature.Ingenious symbolism and efficient and powerful dialogue combine to make the story a representative of Hemingway's short stories.What is especially successful and notable about this book is that it fully represents the author’s style of the"iceberg",where space of multi-profound meanings is hidden in the simple plot.It is so impressive because Ernest Hemingway has succeeded in presenting the deep meaning as close as possible to the readers through the simple and concise words.This paper tries to carry out a research on the narrative rhetoric of Hills Like White Elephants from three aspects.The first part begins with the analysis of its narrator and narrative situation.The second part centers on the focalization.In the last part,the emphasis is put on the narrative modes.
