The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cas...The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cassia,Mentha canadensis,Eugenia aromaticum,Coptis chinensis,Phellodendron amurense,Scutellaria baicalensis,star anise,Kaempferia galanga,Prunus mume,Magnolia sinensis,Terminalia chebula meat,Sophora flavescens,Polygonum cuspidatum,Rhizoma polygonatum,Astragalus membranaceus,Forsythia suspense and...展开更多
[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connec...[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connected into bait vector pGBKTT. The recombined vector was transformed into yeast competent ceils AH109 and carried out self-activated detection and toxicity detection in the au.xotrephic medium. [ Result] Digestion and sequencing results showed that bait vector pGBKT7-Lescpth5 was suecessfully constructed with correct reading frame. Self-activated activity and toxicity detection results showed that bait vector had no self- activated activity on yeast strain AH109, which also had no toxicity on yeast. [Condusion] Bait vector successfully constructed could be used in yeast two-hybrid system, which laid the foundation for screening of cDNA library in the next step.展开更多
By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of re...By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...展开更多
Protein baitsprays were produced from many different sources for the controlling fruit flies in the integrated pest management program around the world.Here,fruit fly baits were formulated based on cost-effective bait...Protein baitsprays were produced from many different sources for the controlling fruit flies in the integrated pest management program around the world.Here,fruit fly baits were formulated based on cost-effective bait mixtures such as local jaggery,brewers spent grain(BSG),apple cider vinegar,wheat bran powder and local red wine with the fresh enzyme from papaya and pineapple.Fruit fly Baits were prepared by mixing the small amount of local detergent as insecticide.Our results indicated that the best efficient fruit fly bait for area wide integrated pest management(AW-IPM)is the BSG-based bait whereas the second efficient bait is local red wine-based bait.The highest fruit fly per trap per day(FTD)value by BSG-based bait traps was discovered.The highest female captured percentage is BSG-based bait with papaya solution.The field analysis of one week and one month old protein-based bait showed that FTD values decreased slightly while only one month old wheat bran-based bait dropped to the base.The BSG-based baits analysis is effective not only female fruit flies but also cockroaches male in Myanmar’s tropical region.Thus our results reveal that the efficient protein-based bait is the fresh BSG-based bait with papaya solution.展开更多
The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effect...The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effects of 0. 04% indoxacarb on nests and worker ants reached 100%; the control effect of 0. 02% and0. 06% indoxacarb on active nests and worker ants were 87. 50% and 100%,respectively; 0. 000 25% and 0. 000 5% fipronil and 0. 05% chlorfenapyr also had significant control effects on active nests and worker ants. At 30 d post administration,the decline rates of ant colony grade treated by 0. 04% and 0. 06% indoxacarb,0. 000 25% fipronil and 0. 05% chlorfenapyr were 100%,100%,91. 44% and 98. 11%,respectively,and the comprehensive control effect reached 97. 18%.展开更多
Six types of termites＇ baits （cartoon, rough tissues, soft toilet tissues, acacia wood, saw dust of building wood and sackcloth） were elicited variable degree of attraction toward Anacanthotermes ochaceus, where th...Six types of termites＇ baits （cartoon, rough tissues, soft toilet tissues, acacia wood, saw dust of building wood and sackcloth） were elicited variable degree of attraction toward Anacanthotermes ochaceus, where the best one was cardboard, followed by soft and rough toilet tissue at mono-choice and multiple-choice tests. Chemical analysis showed that there were variations in the chemical constituents （lignin, cellulose, N, H and C） of the six baits studied. On the other hand, olfactory reactions of harvester termites toward eight volatile oils （four natural medical plants and four commercial terpines） were repellent, but the most repellent one was cineol and the least one was clove oil. The most tested oils were caused inhibition in feeding, except cedar wood. The most toxic oils were cineol followed by thymol.展开更多
基金Supported by National Spark Program(2007EA610021)Tianjin Spark Program(07ZHXHNC05400)~~
文摘The study aimed to discuss baiting effects of 17 kinds of Chinese medicinal plants on Brachydanio rerio.With 100 fish of Brachydanio rerio as tested objects,the average length of the 100 fish was 3.5 cm.Cinnamomum cassia,Mentha canadensis,Eugenia aromaticum,Coptis chinensis,Phellodendron amurense,Scutellaria baicalensis,star anise,Kaempferia galanga,Prunus mume,Magnolia sinensis,Terminalia chebula meat,Sophora flavescens,Polygonum cuspidatum,Rhizoma polygonatum,Astragalus membranaceus,Forsythia suspense and...
基金Supported by Natural Science Foundation of Beijing (6012017)Dr. Funding of Xinjiang Production and Construction Corps~~
文摘[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connected into bait vector pGBKTT. The recombined vector was transformed into yeast competent ceils AH109 and carried out self-activated detection and toxicity detection in the au.xotrephic medium. [ Result] Digestion and sequencing results showed that bait vector pGBKT7-Lescpth5 was suecessfully constructed with correct reading frame. Self-activated activity and toxicity detection results showed that bait vector had no self- activated activity on yeast strain AH109, which also had no toxicity on yeast. [Condusion] Bait vector successfully constructed could be used in yeast two-hybrid system, which laid the foundation for screening of cDNA library in the next step.
基金supported by grants from National Natural Sciences Foundation of China(No.30672227,30600668)"973"Program of China(No.2009CB521800)Joint Research Fund for Overseas Chinese,Hong Kong and Macao Young Scholars(No.30628029)
文摘By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...
文摘Protein baitsprays were produced from many different sources for the controlling fruit flies in the integrated pest management program around the world.Here,fruit fly baits were formulated based on cost-effective bait mixtures such as local jaggery,brewers spent grain(BSG),apple cider vinegar,wheat bran powder and local red wine with the fresh enzyme from papaya and pineapple.Fruit fly Baits were prepared by mixing the small amount of local detergent as insecticide.Our results indicated that the best efficient fruit fly bait for area wide integrated pest management(AW-IPM)is the BSG-based bait whereas the second efficient bait is local red wine-based bait.The highest fruit fly per trap per day(FTD)value by BSG-based bait traps was discovered.The highest female captured percentage is BSG-based bait with papaya solution.The field analysis of one week and one month old protein-based bait showed that FTD values decreased slightly while only one month old wheat bran-based bait dropped to the base.The BSG-based baits analysis is effective not only female fruit flies but also cockroaches male in Myanmar’s tropical region.Thus our results reveal that the efficient protein-based bait is the fresh BSG-based bait with papaya solution.
基金Supported by Project of Liuzhou Science and Technology Bureau"Investigation and Prevention Countermeasures of Fire Ants in Liuzhou City"(2013J020101)
文摘The field control effects of different concentrations of indoxacarb baits,fipronil baits and chlorfenapyr baits on Solenopsis invicta was evaluated. The results showed that at 21 post administration,the control effects of 0. 04% indoxacarb on nests and worker ants reached 100%; the control effect of 0. 02% and0. 06% indoxacarb on active nests and worker ants were 87. 50% and 100%,respectively; 0. 000 25% and 0. 000 5% fipronil and 0. 05% chlorfenapyr also had significant control effects on active nests and worker ants. At 30 d post administration,the decline rates of ant colony grade treated by 0. 04% and 0. 06% indoxacarb,0. 000 25% fipronil and 0. 05% chlorfenapyr were 100%,100%,91. 44% and 98. 11%,respectively,and the comprehensive control effect reached 97. 18%.
文摘Six types of termites＇ baits （cartoon, rough tissues, soft toilet tissues, acacia wood, saw dust of building wood and sackcloth） were elicited variable degree of attraction toward Anacanthotermes ochaceus, where the best one was cardboard, followed by soft and rough toilet tissue at mono-choice and multiple-choice tests. Chemical analysis showed that there were variations in the chemical constituents （lignin, cellulose, N, H and C） of the six baits studied. On the other hand, olfactory reactions of harvester termites toward eight volatile oils （four natural medical plants and four commercial terpines） were repellent, but the most repellent one was cineol and the least one was clove oil. The most tested oils were caused inhibition in feeding, except cedar wood. The most toxic oils were cineol followed by thymol.
文摘红火蚁Solenopsis invicta Buren是全球百种最具危害性的外来入侵物种之一,严重威胁农林业生产、人畜健康、公共安全及生物多样性。精准高效监测红火蚁是其防控的关键,而诱饵诱集是常用的监测手段。本研究在前期已研发的专业监测诱饵内添加5%、10%及15%的NaCl,并测试其储存期及对红火蚁诱集效果的影响,旨在进一步提升诱饵使用效能。结果表明,NaCl浓度越高,诱饵颜色与气味指标降级延迟效应越显著,说明NaCl对诱饵的腐败具有延缓作用。室内测试发现红火蚁的诱集效果总体上随着诱饵储存期的延长而降低,5%NaCl处理在第19天至第25天期间的诱集效果显著高于未添加NaCl的对照,10%NaCl处理仅在第19天、第22天显著,而15%NaCl处理仅在第16天显著。室外测试发现,前20 d NaCl处理与对照差异不显著;第30天,5%NaCl处理的诱集效果显著高于其他处理与对照。综上所述,添加5%NaCl的诱饵在储存期和诱集效果上均表现最佳,可为开发精准高效且低成本的红火蚁监测诱饵提供科学依据。
