Fluorous gold nanoparticle （Fluo-NP）, i.e. gold nanoparticle covered by fluorous ponytail （C8F17-） was pre- pared and found to self-assemble into supra-structures covered by carbohydrates （named supraF-NP）, due ...Fluorous gold nanoparticle （Fluo-NP）, i.e. gold nanoparticle covered by fluorous ponytail （C8F17-） was pre- pared and found to self-assemble into supra-structures covered by carbohydrates （named supraF-NP）, due to the strong fluorous interaction. More importantly, the re-dispersed supraF-NP （diameter around 200 nm） in water pro- vided an alternative strategy to detect carbohydrate-protein interaction in solution by naked eve.展开更多
We report the development of a small interfering RNA （siRNA） delivery vector based on cationic perfluorocarbon nanoemulsions. We have prepared perfluorodecalin （PFD） emulsions with a positive surface charge provid...We report the development of a small interfering RNA （siRNA） delivery vector based on cationic perfluorocarbon nanoemulsions. We have prepared perfluorodecalin （PFD） emulsions with a positive surface charge provided by a fluorinated poly（ethylenimine） （F-PEI）. The fluorinated emulsion （F-PEI@PFD） reduced cytotoxicity of F-PEI and demonstrated effective binding with siRNAs to form nanosized emulsion polyplexes. The prepared emulsion polyplexes enhanced cellular uptake and improved endosomal escape of the siRNA. In addition to increased reporter gene silencing in multiple cancer cell lines, when compared with control F-PEI and PEI polyplexes, the siR_NA emulsion polyplexes showed an excellent resistance to serum deactivation and maintained high activity, even in high-serum conditions. The F-PEI@PFD emulsion polyplexes carrying an siRNA to silence the expression of Bcl2 gene induced apoptosis and inhibited tumor growth in a melanoma mouse model in vivo and showed potential for in vivo ultrasound imaging. This study demonstrates the potential of F-PEI@PFD emulsions as a multifunctional theranostic nanoplatform for safe siRNA delivery, with integrated ultrasound imaging functionality.展开更多
基金The Ministry of Science and Technology of China，the National Natural Science Foundation of China，the Shanghai Rising-Star Program (No.13QA1400600) are acknowledged for their financial supports
文摘Fluorous gold nanoparticle （Fluo-NP）, i.e. gold nanoparticle covered by fluorous ponytail （C8F17-） was pre- pared and found to self-assemble into supra-structures covered by carbohydrates （named supraF-NP）, due to the strong fluorous interaction. More importantly, the re-dispersed supraF-NP （diameter around 200 nm） in water pro- vided an alternative strategy to detect carbohydrate-protein interaction in solution by naked eve.
基金This work was financially supported by the National Science and Technology Major Project （No. 2017YFA0205400）, the Changjiang Scholar program, the National Natural Science Foundation of China （Nos. 81373983 and 81573377）, China Postdoctoral Science Foundation （No. 2016M601923）, and Postgraduate Research ＆ Practice Innovation Program of Jiangsu Province （No. KYCX17_0671）.
文摘We report the development of a small interfering RNA （siRNA） delivery vector based on cationic perfluorocarbon nanoemulsions. We have prepared perfluorodecalin （PFD） emulsions with a positive surface charge provided by a fluorinated poly（ethylenimine） （F-PEI）. The fluorinated emulsion （F-PEI@PFD） reduced cytotoxicity of F-PEI and demonstrated effective binding with siRNAs to form nanosized emulsion polyplexes. The prepared emulsion polyplexes enhanced cellular uptake and improved endosomal escape of the siRNA. In addition to increased reporter gene silencing in multiple cancer cell lines, when compared with control F-PEI and PEI polyplexes, the siR_NA emulsion polyplexes showed an excellent resistance to serum deactivation and maintained high activity, even in high-serum conditions. The F-PEI@PFD emulsion polyplexes carrying an siRNA to silence the expression of Bcl2 gene induced apoptosis and inhibited tumor growth in a melanoma mouse model in vivo and showed potential for in vivo ultrasound imaging. This study demonstrates the potential of F-PEI@PFD emulsions as a multifunctional theranostic nanoplatform for safe siRNA delivery, with integrated ultrasound imaging functionality.
