The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolat...The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolates with the attributes of P.fluorescens out of fifteen local isolates that are represented by the following codes and sequences(P.f9,P.f8,P.f6,P.f5,P.f4,P.f2,P.f1,P.f14,P.f13,P.f11).Results also confirmed the diagnosis of bacterial isolates by biochemical and molecular tests using a specialized primer to amplify the bp698 region of the 16S ribosomal RNA gene,approved by Macrogen/Korea.The test efficiency in dissolving solid phosphate by P.fluorescens bacteria showed that the most effective is the(P.f1)isolate,giving the highest score effectiveness in mineral phosphate dissolution by the diameter of the clear zone around the colony,which was effective in phosphate dissolution up to 6.95 mm.The efficiency of the Nitrogen Fixation Test showed that the isolate(P.f5)scored the highest nitrogen-fixing efficiency amount with a value of 6.81 mg L^(-1).The quantitative amount of the hormone for each of Auxins,Cytokinins,and Gibberellins was assayed;the results with isolate(P.f1)for IAA(Auxins)gave a concentration up to 28.6μg ml^(-1),which was the most,while the production of GA3 by isolate(P.f1)gave the maximum value of 36.7μg ml^(-1),and for synthesis of the hormone of Cytokinins represented by isolate(P.f2),the highest value in the production of Cytokinins hormone was recorded at 26.3μg ml^(-1).展开更多
Structured-illumination reflectance imaging(SIRI)provides a new means for food quality detection.This original work investigated the capability of(SIRI)technique coupled with multivariate chemometrics to evaluate the ...Structured-illumination reflectance imaging(SIRI)provides a new means for food quality detection.This original work investigated the capability of(SIRI)technique coupled with multivariate chemometrics to evaluate the microbial contamination in pork inoculated with Pseudomonas fluorescens and Brochothrix thermosphacta during storage at different temperatures.The prediction performances based on different spectrum and the textural features of direct component and amplitude component images demodulated from the SIRI pattern,as well as their data fusion were comprehensively compared.Based on the full wavelength spectrum(420-700 nm)of amplitude component images,the orthogonal signal correction coupled with support vector machine regression provided the best predictions of the number of P.fluorescens and B.thermosphacta in pork,with the determination coefficients of prediction(R_(p)^(2))values of 0.870 and 0.906,respectively.Besides,the prediction models based on the amplitude component or direct component image textural features and the data fusion models using spectrum and textural features from direct component and amplitude component images cannot significantly improve their prediction accuracy.Consequently,SIRI can be further considered as a potential technique for the rapid evaluation of microbial contaminations in pork meat.展开更多
Spraying 1-2 mmol/L solution of NaHSO 3 on rice ( Oryza sativa L.) leaves resulted in the enhancement of net photosynthetic rate for more than three days. It was also observed that NaHSO 3 application caused incr...Spraying 1-2 mmol/L solution of NaHSO 3 on rice ( Oryza sativa L.) leaves resulted in the enhancement of net photosynthetic rate for more than three days. It was also observed that NaHSO 3 application caused increases both in ATP content in leaves and the millisecond_delayed light emission of leaves. The increase in net photosynthetic rate caused by NaHSO 3 treatment was similar to that by PMS (phenazine methosulfate) treatment. The grain yield of treated rice was enhanced approximately by 10% after duplicated application of NaHSO 3 in milk_ripening stage. It is suggested that the enhancement of photosynthesis by NaHSO 3 treatment resulted from the effect of increasing ATP supplement. Concomitant with an increase in the photosynthetic rate and ATP content in leaves, the transient increase in chlorophyll fluorescence after the termination of actinic light, which could be used as an index of the cyclic electron flow, was also enhanced by low concentration of NaHSO 3 treatment. Basing on these results it is proposed that the increase in rice photosynthesis caused by low concentrations of NaHSO 3 could be due to the stimulation of the cyclic electron flow around PSⅠ which in turn the enhancement of the coupled photophosphorylation and photosynthesis.展开更多
A new trinuclear nickel complex,[Ni3（pdc）3（2,2＇-bipy）3（H2O）2]·2H2O（H2pdc = pyridine 2,6-dicarboxylic acid,2,2＇-bipy = 2,2＇-bipyridine） has been hydrothermally synthesized and characterized by IR,elemen...A new trinuclear nickel complex,[Ni3（pdc）3（2,2＇-bipy）3（H2O）2]·2H2O（H2pdc = pyridine 2,6-dicarboxylic acid,2,2＇-bipy = 2,2＇-bipyridine） has been hydrothermally synthesized and characterized by IR,elemental analysis and X-ray diffraction methods.Crystal data for this complex：monoclinic,space group P21/n,a = 21.206（4）,b = 10.002（2）,c = 28.066（6）,β = 108.18（3）°,C51H41N9Ni3O16,Mr = 1212.06,V = 5.656（2） nm3,Dc = 1.423 g·cm-3,μ（MoKα） = 1.062 mm-1,Z = 4,F（000） = 2488,GOOF = 1.034,the final R = 0.0543 and wR = 0.1237 for 6149 observed reflections with Ⅰ 〉 2σ（Ⅰ）.In the complex,three nickel（Ⅱ） ions are bridged by the pyridine 2,6-dicarboxylic acid groups,and all nickel（Ⅱ） ions are seven-coordinated by nitrogen atoms of 2,2＇-bipyridine and pyridine 2,6-dicarboxylic acid and oxygen atoms from pyridine 2,6-dicarboxylic and water to adopt a severely distorted pentagonal bipyramidal geometry.The emission and excitation peaks of the complex in ethanol solutions are located at 336 and 316 nm,respectively.展开更多
目的:用已知细菌革兰阴性、阳性菌双重16S r RNA基因荧光定量PCR方法(q-PCR)应用于妇产科患者菌血症的诊断,探讨该方法检测妇产专科患者菌血症的临床应用价值。方法:2013年1月~2014年12月对100例疑为全身感染处于菌血症状态的住院分娩...目的:用已知细菌革兰阴性、阳性菌双重16S r RNA基因荧光定量PCR方法(q-PCR)应用于妇产科患者菌血症的诊断,探讨该方法检测妇产专科患者菌血症的临床应用价值。方法:2013年1月~2014年12月对100例疑为全身感染处于菌血症状态的住院分娩孕产妇进行常规血液培养,同时用细菌革兰阴性、阳性菌双重16S r RNA基因q-PCR方法检测,分析2种方法诊断菌血症的阳性率、敏感性和特异性。结果 :通过q-PCR方法检测菌血症阳性率44%,血液培养阳性率16%,两者差异有统计学意义(P<0.01);菌血症临床诊断阳性率为37%,与q-PCR法阳性率存在显著性差异(P<0.01)。以血液培养阳性和(或)临床诊断菌血症的标准作为对照,q-PCR方法诊断敏感性为89.2%,特异性82.5%。结论:细菌革兰阴性、阳性菌双重16S r RNA基因q-PCR方法检测妇产科菌血症的阳性率高于血液培养,可快速为孕产妇患者感染提供早期、敏感的病原学诊断依据。展开更多
New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle ...New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.展开更多
文摘The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolates with the attributes of P.fluorescens out of fifteen local isolates that are represented by the following codes and sequences(P.f9,P.f8,P.f6,P.f5,P.f4,P.f2,P.f1,P.f14,P.f13,P.f11).Results also confirmed the diagnosis of bacterial isolates by biochemical and molecular tests using a specialized primer to amplify the bp698 region of the 16S ribosomal RNA gene,approved by Macrogen/Korea.The test efficiency in dissolving solid phosphate by P.fluorescens bacteria showed that the most effective is the(P.f1)isolate,giving the highest score effectiveness in mineral phosphate dissolution by the diameter of the clear zone around the colony,which was effective in phosphate dissolution up to 6.95 mm.The efficiency of the Nitrogen Fixation Test showed that the isolate(P.f5)scored the highest nitrogen-fixing efficiency amount with a value of 6.81 mg L^(-1).The quantitative amount of the hormone for each of Auxins,Cytokinins,and Gibberellins was assayed;the results with isolate(P.f1)for IAA(Auxins)gave a concentration up to 28.6μg ml^(-1),which was the most,while the production of GA3 by isolate(P.f1)gave the maximum value of 36.7μg ml^(-1),and for synthesis of the hormone of Cytokinins represented by isolate(P.f2),the highest value in the production of Cytokinins hormone was recorded at 26.3μg ml^(-1).
基金supported by Key Research&Development Program of Jiangsu Province in China(BE2020693)Major Project of Science and Technology of Anhui Province(201903a06020010)+1 种基金Joint Key Project of Science and Technology Innovation of Yangtze River Delta in Anhui Province(202004g01020009)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Structured-illumination reflectance imaging(SIRI)provides a new means for food quality detection.This original work investigated the capability of(SIRI)technique coupled with multivariate chemometrics to evaluate the microbial contamination in pork inoculated with Pseudomonas fluorescens and Brochothrix thermosphacta during storage at different temperatures.The prediction performances based on different spectrum and the textural features of direct component and amplitude component images demodulated from the SIRI pattern,as well as their data fusion were comprehensively compared.Based on the full wavelength spectrum(420-700 nm)of amplitude component images,the orthogonal signal correction coupled with support vector machine regression provided the best predictions of the number of P.fluorescens and B.thermosphacta in pork,with the determination coefficients of prediction(R_(p)^(2))values of 0.870 and 0.906,respectively.Besides,the prediction models based on the amplitude component or direct component image textural features and the data fusion models using spectrum and textural features from direct component and amplitude component images cannot significantly improve their prediction accuracy.Consequently,SIRI can be further considered as a potential technique for the rapid evaluation of microbial contaminations in pork meat.
文摘Spraying 1-2 mmol/L solution of NaHSO 3 on rice ( Oryza sativa L.) leaves resulted in the enhancement of net photosynthetic rate for more than three days. It was also observed that NaHSO 3 application caused increases both in ATP content in leaves and the millisecond_delayed light emission of leaves. The increase in net photosynthetic rate caused by NaHSO 3 treatment was similar to that by PMS (phenazine methosulfate) treatment. The grain yield of treated rice was enhanced approximately by 10% after duplicated application of NaHSO 3 in milk_ripening stage. It is suggested that the enhancement of photosynthesis by NaHSO 3 treatment resulted from the effect of increasing ATP supplement. Concomitant with an increase in the photosynthetic rate and ATP content in leaves, the transient increase in chlorophyll fluorescence after the termination of actinic light, which could be used as an index of the cyclic electron flow, was also enhanced by low concentration of NaHSO 3 treatment. Basing on these results it is proposed that the increase in rice photosynthesis caused by low concentrations of NaHSO 3 could be due to the stimulation of the cyclic electron flow around PSⅠ which in turn the enhancement of the coupled photophosphorylation and photosynthesis.
基金Supported by the Science and Technology Fund of Hengyang Science and Technology Bureau (No. 2008KS035)the Construct Program of the Key Discipline in Hunan Province
文摘A new trinuclear nickel complex,[Ni3（pdc）3（2,2＇-bipy）3（H2O）2]·2H2O（H2pdc = pyridine 2,6-dicarboxylic acid,2,2＇-bipy = 2,2＇-bipyridine） has been hydrothermally synthesized and characterized by IR,elemental analysis and X-ray diffraction methods.Crystal data for this complex：monoclinic,space group P21/n,a = 21.206（4）,b = 10.002（2）,c = 28.066（6）,β = 108.18（3）°,C51H41N9Ni3O16,Mr = 1212.06,V = 5.656（2） nm3,Dc = 1.423 g·cm-3,μ（MoKα） = 1.062 mm-1,Z = 4,F（000） = 2488,GOOF = 1.034,the final R = 0.0543 and wR = 0.1237 for 6149 observed reflections with Ⅰ 〉 2σ（Ⅰ）.In the complex,three nickel（Ⅱ） ions are bridged by the pyridine 2,6-dicarboxylic acid groups,and all nickel（Ⅱ） ions are seven-coordinated by nitrogen atoms of 2,2＇-bipyridine and pyridine 2,6-dicarboxylic acid and oxygen atoms from pyridine 2,6-dicarboxylic and water to adopt a severely distorted pentagonal bipyramidal geometry.The emission and excitation peaks of the complex in ethanol solutions are located at 336 and 316 nm,respectively.
文摘目的:用已知细菌革兰阴性、阳性菌双重16S r RNA基因荧光定量PCR方法(q-PCR)应用于妇产科患者菌血症的诊断,探讨该方法检测妇产专科患者菌血症的临床应用价值。方法:2013年1月~2014年12月对100例疑为全身感染处于菌血症状态的住院分娩孕产妇进行常规血液培养,同时用细菌革兰阴性、阳性菌双重16S r RNA基因q-PCR方法检测,分析2种方法诊断菌血症的阳性率、敏感性和特异性。结果 :通过q-PCR方法检测菌血症阳性率44%,血液培养阳性率16%,两者差异有统计学意义(P<0.01);菌血症临床诊断阳性率为37%,与q-PCR法阳性率存在显著性差异(P<0.01)。以血液培养阳性和(或)临床诊断菌血症的标准作为对照,q-PCR方法诊断敏感性为89.2%,特异性82.5%。结论:细菌革兰阴性、阳性菌双重16S r RNA基因q-PCR方法检测妇产科菌血症的阳性率高于血液培养,可快速为孕产妇患者感染提供早期、敏感的病原学诊断依据。
文摘New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.
