Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth ...Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.展开更多
A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelect...A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship（R2=0.9977） with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT（3-（4,5-dimethylthiazol-2-yl）-2,5diphenyl tetrazolium bromide） assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.展开更多
In the late 1980s,superparamagnetic iron oxide nanoparticles（SPIO）moved into focus as contrast agents in magnetic resonance imaging（MRI）,due to their strong relaxivity and resulting higher resolution of images.At ...In the late 1980s,superparamagnetic iron oxide nanoparticles（SPIO）moved into focus as contrast agents in magnetic resonance imaging（MRI）,due to their strong relaxivity and resulting higher resolution of images.At the time,no one anticipated their high potential in basic research or for medical diagnostic andtreatment. Since then, SPIO have been evaluated notonly as spe- cific markers for MRI, but also for cell labeling and tracking （Li et al., 2013）.展开更多
A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in P...A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in PBS buffer. The excellent chemoselectivity of ADNO for NO over other ROS/RNS （reactive oxygen species or nitrogen species） and common metal ions was observed. Moreover, ADNO has been successfully applied in fluorescence imaging of NO of living cells using both one-photon microscopy （OPM） and two-~hoton microscopy （TPM）,展开更多
Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes b...Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylene- diamine as a NO reaction site and 4-（2-aminoethyl）-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity （4.57 μmol/L） toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.展开更多
The fluorescence of graphene oxide quantum dots （GOQDs） that are infiltrated into porous silicon （PSi） is investigated. By dropping activated GOQDs solution onto silanized PSi samples, GOQDs are successfully in- f...The fluorescence of graphene oxide quantum dots （GOQDs） that are infiltrated into porous silicon （PSi） is investigated. By dropping activated GOQDs solution onto silanized PSi samples, GOQDs are successfully in- filtrated into a PSi device. The results indicate that the intensity of the fluorescence of the GOQD-inflltrated multilayer with a high reflection band located at its fluorescence spectra scope is approximately double that of the single layer sample. This indicates that the multilayer GOQD-infiltrated PSi substrate is a suitable material for the preparation of sensitive photoluminescence biosensors.展开更多
基金support for this work by the Archimedes project 3.2.1101.12-0011supported in part by the institutional research funding IUT (IUT 2-26)of the Estonian Ministry of Education and Research.
文摘Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.
基金Funded by the National Natural Science Foundation of China(Nos.50802069,81100890,51272191)the Fundamental Research Funds for the Central Unversities(WUT:2013-IV-010)the Students Innovation and Entrepreneurship Training Program of WHUT(20141049701012)
文摘A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship（R2=0.9977） with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT（3-（4,5-dimethylthiazol-2-yl）-2,5diphenyl tetrazolium bromide） assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.
基金supported by deutsche Forschungsgemeinschaft Grant Klinische Forschungsgruppe 213 to JG
文摘In the late 1980s,superparamagnetic iron oxide nanoparticles（SPIO）moved into focus as contrast agents in magnetic resonance imaging（MRI）,due to their strong relaxivity and resulting higher resolution of images.At the time,no one anticipated their high potential in basic research or for medical diagnostic andtreatment. Since then, SPIO have been evaluated notonly as spe- cific markers for MRI, but also for cell labeling and tracking （Li et al., 2013）.
基金the National Natural Science Foundation of China(Nos.21102148 and 21125205)National Basic Research Program of China(No.2011CB935800)the State Key Laboratory of Fine Chemicals,Department of Chemical Engineering,Dalian University of Technology for financial supports
文摘A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in PBS buffer. The excellent chemoselectivity of ADNO for NO over other ROS/RNS （reactive oxygen species or nitrogen species） and common metal ions was observed. Moreover, ADNO has been successfully applied in fluorescence imaging of NO of living cells using both one-photon microscopy （OPM） and two-~hoton microscopy （TPM）,
基金financial supports from the National Natural Science Foundation of China (Nos. 21276251, 21506206, 21402191, 21502189)the 100 talents program funded by Chinese Academy of Sciences, Dalian Cultivation Fund for Distinguished Young Scholars (Nos. 2014J11JH130, 2015J12JH205)the National Science Fund for Excellent Young Scholars (No. 21422606)
文摘Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylene- diamine as a NO reaction site and 4-（2-aminoethyl）-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity （4.57 μmol/L） toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.
基金supported by the National Natural Science Foundation of China(Nos.61575168 and 61265009)the Xinjiang Science and Technology Project(No.201412112)
文摘The fluorescence of graphene oxide quantum dots （GOQDs） that are infiltrated into porous silicon （PSi） is investigated. By dropping activated GOQDs solution onto silanized PSi samples, GOQDs are successfully in- filtrated into a PSi device. The results indicate that the intensity of the fluorescence of the GOQD-inflltrated multilayer with a high reflection band located at its fluorescence spectra scope is approximately double that of the single layer sample. This indicates that the multilayer GOQD-infiltrated PSi substrate is a suitable material for the preparation of sensitive photoluminescence biosensors.
