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Three-Stage Six-Step Flue-Curing Technology for Viginia Tobacco Leaves and Its Application in China 被引量:1
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作者 Hongfeng Wang Houlong Jiang +3 位作者 Chen Xu Daibin Wang Chao Yang Bojun Wang 《Journal of Agricultural Science and Technology(A)》 2016年第4期232-238,共7页
In order to overcome the problem that the leaf yellowing stage in tobacco curing reduces the fragrance of the product,this study introduced the principle and key parameters of a three-stage six-step curing technology.... In order to overcome the problem that the leaf yellowing stage in tobacco curing reduces the fragrance of the product,this study introduced the principle and key parameters of a three-stage six-step curing technology.The field trail was conducted in Wulong county of Chongqing city in 2010,and Virginia K326 was the tested cultivar.The middle leaves were harvested and plaited after maturity,and then cured with the three-stage six-step curing technology and traditional three-stage curing technology,respectively.Three replicates were established for each of the two curing technologies.Then,chemical constituents,aromatic substances and smoking quality were analyzed.The results show that compared with the traditional three-stage curing technology,the three-stage six-step curing technology produces more suitable ratios of chemical constituents,higher aromatic component content and higher smoking quality grade of tobacco leaves.Therefore,the continuous improvement of corollary equipment and matching technologies is expected to widen the applications of the three-stage six-step curing technology in tobacco and provide a strong technical support for the development of the tobacco industry. 展开更多
关键词 flue-cured tobacco curing technology quality index.
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Advances in interdisciplinary ocean geoscience and technology
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作者 Jian LIN Wule LIN +1 位作者 Zhiyuan ZHOU Fan ZHANG 《Science China Earth Sciences》 2026年第2期463-477,共15页
Ocean geoscience is a highly integrated and interdisciplinary field that plays a critical role in understanding the interaction between Earth’s lithosphere,hydrosphere,atmosphere,biosphere,and anthroposphere.Recent y... Ocean geoscience is a highly integrated and interdisciplinary field that plays a critical role in understanding the interaction between Earth’s lithosphere,hydrosphere,atmosphere,biosphere,and anthroposphere.Recent years have seen tremendous progress in global ocean research,driven by rapid advancements in deep-sea manned and unmanned submersibles,ocean drilling,seafloor observatories,big data assimilation,and supercomputing simulations.Representative examples of breakthroughs are highlighted in this work:(1)Probing sub-seafloor processes.A 10,000-meter ocean-bottom seismometer array has achieved high-resolution imaging of the deepest ocean on the Earth-the Challenger Deep of the Mariana Trench,revealing the role of key tectonic and hydrological processes within the subduction zone.The first sub-ice seafloor seismic and magnetotelluric experiments were successfully conducted at the Arctic Gakkel Ridge,providing significant insights into the dynamics of ultraslow seafloor spreading.(2)Exploration of seafloor resources.Near-seafloor investigations employing underwater robotics and multi-sensor systems have been carried out in areas of hydrothermal vents and cold seeps at global locations,including the Southwest Indian Ridge.These efforts have combined geophysical,oceanographic,chemical,and biological observations with extensive seafloor sampling.(3)Interdisciplinary research of complex catastrophic events.High-resolution simulations integrating ocean observations with supercomputing modeling have made it possible to fully model earthquake-induced seafloor deformation,tsunami propagation,and ocean basin-scale transport of the Fukushima Power Plant-derived radionuclides associated with the 2011 Tohoku earthquake.Among the world’s three major oceans,the Indian Ocean is still relatively underexplored.Major scientific challenges include elucidating crust-mantle interaction,air-sea dynamic coupling,large-scale marine hazards,and responses of ecosystems to major environmental changes,all of which require interdisciplinary collaboration.Future efforts should focus on developing intelligent unmanned observation platform systems,big data and digital twins,and AI-driven hazard modeling.Meanwhile,higher educational reforms should emphasize fostering a new generation of students and young scientists with a solid background and strong critical analysis skills to accelerate technological innovation. 展开更多
关键词 Ocean geoscience Cutting-edge ocean technology Deep-sea science and technology Multi-sphere coupling Sustainable development
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Research on the Optimal Allocation of Community Elderly Care Service Resources Based on Big Data Technology
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作者 Shuying Li 《Journal of Clinical and Nursing Research》 2026年第1期241-246,共6页
With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service... With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service resources,uneven distribution,and prominent supply-demand contradictions have seriously affected service quality.Big data technology,with core advantages including data collection,analysis and mining,and accurate prediction,provides a new solution for the allocation of community elderly care service resources.This paper systematically studies the application value of big data technology in the allocation of community elderly care service resources from three aspects:resource allocation efficiency,service accuracy,and management intelligence.Combined with practical needs,it proposes optimal allocation strategies such as building a big data analysis platform and accurately grasping the elderly’s care needs,striving to provide operable path references for the construction of community elderly care service systems,promoting the early realization of the elderly care service goal of“adequate support and proper care for the elderly”,and boosting the high-quality development of China’s elderly care service industry. 展开更多
关键词 Big data technology COMMUNITY Elderly care Service resources
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Tracking a High-Tech Transition--How technology is powering Guangdong’s manufacturing transformation
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作者 HU FAN 《ChinAfrica》 2026年第1期30-32,共3页
The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them w... The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them was not a typical exhibition hall,but a building shaped like a gleaming stainless-steel cooking pot. 展开更多
关键词 othello kitchenware museum technology industrial strength high tech transition guangdong manufacturing transformation
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Setting the standard! The technology that shapes 40% of the world's denim finishing
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《China Textile》 2026年第1期44-44,共1页
Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision... Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision has become a global reality.Today,more than 40 percent of all jeans produced worldwide are made using technologies developed by the Valencia-based company.What started in a small laboratory in Valencia(Spain),with a pioneering team and a visionary idea,has evolved into a global benchmark in sustainable innovation.From the beginning,Jeanologia believed in technology as a driver of change,introducing solutions that eliminated harmful practices and opened the door to a new,cleaner way of making garments. 展开更多
关键词 technological transformation environmental impact textile industry garment production DENIM jeanologia sustainability innovation
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In-situ synthesis of rare earth Y-type zeolite enhanced by high-gravity technology
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作者 Lang Xu Wenhui Hou +3 位作者 Houmei You Baochang Sun Guangwen Chu Jianfeng Chen 《Chinese Journal of Chemical Engineering》 2026年第1期240-248,共9页
Rare earth(RE)Y-type zeolite was synthesized in situ by acidic co-hydrolysis route and hydrothermal method.The key process parameters were optimized based on the RE utilization rate.The effect of inducing a rotating p... Rare earth(RE)Y-type zeolite was synthesized in situ by acidic co-hydrolysis route and hydrothermal method.The key process parameters were optimized based on the RE utilization rate.The effect of inducing a rotating packed bed(RPB)in premixing and crystallization on crystallinity and RE utilization rate was further investigated.The results indicate that lanthanide(La)cations are successfully introduced into the sodalite cage of Y-type zeolite.The optimized conditions are that the molar ratio of Si/La is 150,premixing for 5 h,crystallization at 90℃ for 18 h,and calcination at 550℃ for 3.5 h.At this stage,the RE utilization rate reaches 74.5%.Compared with the conventional stirred tank reactor(STR),RPB can effectively shorten the premixing time and crystallization time by 4.3 h and 6 h,improve the crystallinity by 23%and RE utilization rate by 7.5%.The RE utilization rate is more than 80%by RPB,surpassing the effectiveness of using the one-exchange one-calcination process in the traditional liquid ion exchange process.It is expected to provide a reference for the in-situ efficient and green synthesis of RE zeolite. 展开更多
关键词 High-gravity technology Rare earth zeolite In-situ synthesis Rare earth utilization rate
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Reform of Teacher-Machine-Student Interactive Teaching Model of“HarmonyOS Development Technology”Course Based on Congyou Platform
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作者 Yixian Liu Jun Guo +1 位作者 Dongming Chen Zhiliang Zhu 《计算机教育》 2026年第3期158-166,共9页
Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model... Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model based on the Congyou platform.Through the building block curriculum system,the HarmonyOS technology stack is decoupled into dynamic capability units,and a multi-disciplinary cross-case library is jointly built with Huawei,which significantly improves the synchronization of teaching content and industrial technology.This paper innovatively designs an AI collaborative teaching system,which employs knowledge graphs to plan learning paths,utilizes virtual equipment clusters to simulate development environments,and establishes a“diagnosis-feedback-enhancement”closed loop through AI-based review,thereby effectively improving students’development efficiency and code reuse rate.A three-dimensional evaluation model integrating task outcomes,process performance,and innovation is constructed,incorporating indicators such as code standardization and an innovation index to strengthen the cultivation of engineering thinking and innovative ability.Furthermore,a data-driven support platform is built to generate student competency profiles,open up the“credit-competency-certification”pathway,promote the transformation of course achievements into contributions to the Huawei ecosystem,and significantly shorten the job adaptation cycle for graduates.The research results provide a replicable paradigm for the cultivation of domestic operating system talents. 展开更多
关键词 Interactive teaching model HarmonyOS development technology Building-block curriculum system Domestic software ecosystem
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Chinese Rural Women's Social Media Use: An Empirical Study Adopting the Technology Acceptance Model
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作者 Zhang Hui Mahadevan Krishnan 《Contemporary Social Sciences》 2026年第1期104-122,共19页
Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Accept... Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Acceptance Model(TAM). Employing quantitative research methods, the study conducted empirical tests based on 367 valid questionnaires using Partial Least Squares Structural Equation Modeling(PLS-SEM) via SmartPLS 4.0 software. Results indicate that significant associations exist between perceived ease of use, perceived usefulness, attitudes toward use, behavioral intention, and actual usage behavior. Specifically, the study finds that rural women's perceived ease of use of social media has a significant and positive influence on both perceived usefulness and attitudes toward use. Perceived usefulness further significantly promotes attitudes toward use and behavioral intention. Moreover, positive attitudes toward usage and strong behavioral intentions were effectively converted into actual social media usage behaviors. This study not only validates the applicability and explanatory power of the TAM model in understanding the digital behavior of Chinese rural women but also provides quantitative evidence for how social media enhances their “digital visibility.” These findings offer practical insights for governments and platform providers to optimize user experiences and strengthen digital skills training. Despite its limitations, including a cross-sectional design and a regional sample, this research holds significant theoretical and practical implications. 展开更多
关键词 Chinese rural women social media technology Acceptance Model(TAM) digital visibility
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Between Themis and Prometheus:a history of the ethics of science and technology
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作者 Jia-Yun Chen Zong-Zhen Wu +7 位作者 Qiang-Yu Xiang Lu Zhang Jia-Lin Diao Ya-Hui Zhang Shen-Shen Yan Kun-Lan Zuo Jian-Liang Huang Huan Liu 《History and Philosophy of Medicine》 2026年第1期49-59,共11页
Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide ... Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide technology for good and prevent and control technological risks has become an important issue of global concern.Research on science and technology ethics is dedicated to integrating ethical theories into governance practices and constructing ethical models that adapt to the development of the times.Methods:This article systematically reviews the six core approaches of scientific and technological ethics thought,including technological autonomy and political philosophy criticism,responsibility ethics and intergenerational obligations,technological intermediation and the integration of life and the world,ethical principles and normative frameworks,participatory governance and ethical practice innovation,as well as domain-specific ethical norms,thereby constructing an ethical analysis framework applicable to medical technology risks.And cross-analysis was conducted by taking medical events such as gene editing and xenotransplantation as examples.Results:Research shows that a single ethical approach has limitations in addressing complex medical ethical challenges,while the six approaches are complementary and synergistic.By criticizing technological autonomy,establishing a responsibility ethics orientation,setting the bottom line of ethical principles,promoting participatory governance,formulating domain norms,and continuously reflecting on the intermediary nature of technology,a multi-level and dynamically adaptive governance system for scientific and technological ethics can be constructed.Conclusion:The key to addressing contemporary medical ethics challenges lies in the comprehensive application of science and technology ethics theories and the integration of ethical considerations throughout the entire process of scientific and technological research and development.In the future,a governance framework that adapts to the development of new technologies should be established to promote cross-cultural and cross-disciplinary ethical dialogue and public participation,ensuring that scientific and technological innovation always serves the dignity of human life and overall well-being. 展开更多
关键词 ethics of science and technology history of science ethical governance responsible innovation medical ethics
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Teaching Reform of Chinese Medicine Processing Technology Course under the Integration of Technical and Higher Vocational Education
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作者 Lianfang LI 《Medicinal Plant》 2026年第1期72-77,82,共7页
Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This pap... Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This paper analyzes the existing problems in the current teaching of Chinese Medicine Processing Technology courses,discusses the necessity of reforming the teaching model under the context of integration,and proposes the construction of a"Dual-Capability Progression,Six-Dimensional Empowerment"teaching model.The aim is to enhance the teaching quality of Chinese Medicine Processing Technology courses and cultivate high-quality skilled talents in Chinese medicine processing who can meet industry demands. 展开更多
关键词 Technical education Higher vocational education Integrated development Chinese Medicine Processing technology Course Teaching reform
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Optical lateral flow immune assay technology for body fluid sensing
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作者 Chen Liu Tianqi Zhao +5 位作者 Jialing Zhou Xiaoyun Hu Dinghao Pan Jinlong Li Wei Li Zhihui Dai 《Chinese Chemical Letters》 2026年第1期106-115,共10页
Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly b... Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly becoming the preferred choice of physicians and patients for point-of-care testing due to its simplicity,cost-effectiveness,and rapid detection.Observing the optical signal change from the colloidal gold of the traditional LFIA strip has been widely applied for various biomarkers detection in body fluids.Despite the significant progress,rapid real-time detection of color changes in the colloidal gold by the naked eye still faces many limitations,such as large errors and the inability to quantify and accurately detect.New optical LFIA strip technology has emerged in recent years to extend its application scenarios for achieving quantitative detection such as fluorescence,afterglow,and chemiluminescence.Herein,we summarized the development of optical LFIA technology from single to hyphenated optical signals for biomarkers detection in body fluids from invasive and non-invasive sources.Moreover,the challenge and outlook of optical LFIA strip technology are highlighted to inspire the designing of next-generation diagnostic platforms. 展开更多
关键词 Optical signal Lateral flow immune assay Hyphenated optical technology Body fluid sensing Point-of-care testing
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Application of Whole Slide Imaging Technology in Clinical Pathological Diagnosis
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作者 Zhenjie Wang Jianguo Huai 《Journal of Clinical and Nursing Research》 2026年第1期21-26,共6页
Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass patho... Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass pathological sections into complete digital images through high-resolution scanning, it provides a new method for pathological diagnosis. Based on this, this paper studies the application of WSI technology in clinical pathological diagnosis, elaborates on its application value, analyzes the current application status, and proposes corresponding application countermeasures, aiming to provide reference for the standardized and popularized development of this technology in clinical pathological diagnosis. 展开更多
关键词 Whole slide imaging(WSI)technology Clinical pathological diagnosis Application value Current status Application countermeasures
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Defence Technology Aims and Scope
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《Defence Technology(防务技术)》 2026年第2期F0002-F0002,共1页
Defence Technology(ISSN 2214-9147(O);2096-3459(P)),sponsored by China Ordnance Society,is published monthly and aims to become one of the well-known comprehensive journals in the world,which reports on the breakthroug... Defence Technology(ISSN 2214-9147(O);2096-3459(P)),sponsored by China Ordnance Society,is published monthly and aims to become one of the well-known comprehensive journals in the world,which reports on the breakthroughs in defence technology by building up an international academic exchange platform for the defence technology related research.It publishes original research papers having direct bearing on defence,with a balanced coverage on analytical,experimental,numerical simulation and applied investigations.It covers various disciplines of science,technology and engineering. 展开更多
关键词 defence technology applied investigation original research papers analytical methods academic exchange platform breakthroughs experimental methods international academic exchange
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Low-temperature bonding of W90 tungsten heavy alloy using Sn−2Al filler metal by ultrasonic-assisted coating technology
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作者 Xiao-guo SONG Xu-dong ZHANG +3 位作者 Wei FU Xiao-yu TIAN Guang-dong WU Sheng-peng HU 《Transactions of Nonferrous Metals Society of China》 2026年第1期259-272,共14页
The Sn−2Al filler metal was utilized to bond W90 tungsten heavy alloys by the ultrasonic-assisted coating technology in atmospheric environment at 250℃.The effects of ultrasonic power and ultrasonic time on microstru... The Sn−2Al filler metal was utilized to bond W90 tungsten heavy alloys by the ultrasonic-assisted coating technology in atmospheric environment at 250℃.The effects of ultrasonic power and ultrasonic time on microstructure and interfacial strength of Sn−2Al/W90 interface were investigated.The ultrasound improved the wettability of Sn−2Al filler metal on W90 surface.As the ultrasonic power increased and ultrasonic time increased,the size of Al phase in seam decreased.The maximum value of Sn−2Al/W90 interfacial strength reached 30.1 MPa.Based on the acoustic pressure simulation and bubble dynamics,the intensity of cavitation effect was proportional to ultrasonic power.The generated high temperature and high pressure by cavitation effect reached 83799.6 K and 1.26×10^(14) Pa,respectively. 展开更多
关键词 W−Ni−Fe alloy Sn−Al filler metal ultrasonic-assisted coating technology acoustic pressure CAVITATION bubble dynamics
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A review of microfluidic technology for CO_(2)sequestration in saline aquifers
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作者 Yiyan Zhong Qi Li +2 位作者 Wenbin Gao Yiping Wen Yao Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期810-829,共20页
In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction proces... In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction processes in porous media.This review examines the application of microfluidic technology in CO_(2)sequestration in saline aquifers,emphasizing the advantages of saline aquifer for geological sequestration,including safety,high storage capacity,stability,and cost-effectiveness.The materials used for microfluidic chips and the design of microchannels are systematically reviewed,offering forward-looking recommendations for chip selection and microchannel characterization in future research on CO_(2)sequestration in saline aquifer.Based on a detailed analysis of advancements in microfluidic technology,this review highlights key findings related to CO_(2)trapping mechanisms,salt precipitation,and CO_(2)-water-rock chemical interactions within saline aquifers.Although microfluidic technology shows great promise in these areas,this review identifies limitations in current studies and outlines future research directions,aiming to promote further innovation and broader application of microfluidic technology in the field of CO_(2)sequestration in saline aquifer. 展开更多
关键词 Microfluidic technology Chip materials Microchannel fabrication CO_(2)trapping mechanisms Salt precipitation CO_(2)-water-rock interaction
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Major Cultivation Technology of HQ No.1, a New Flue-cured Tobacco Cultivar
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作者 李文宇 李爱华 《Agricultural Science & Technology》 CAS 2015年第3期497-499,共3页
The transplanting period, density, the number of left leaves and fertilizer amounts of HQ No.1 were explored in Hengyang. The results showed that with transplanting periods of March 5-March 15, and planting space of 1... The transplanting period, density, the number of left leaves and fertilizer amounts of HQ No.1 were explored in Hengyang. The results showed that with transplanting periods of March 5-March 15, and planting space of 120 cmx(50-60) cm, agronomic and economic characters of tobaccos showed insignificant differences. Specifically, plant height was growing upon the number of left leaf and fertilizer amount; tobacco yield and output value kept increasing upon fertilizer, and both reached maximums with pure N at 195 kg/hm^2, showing significant differences with the treatment of pure N at 135 kg/hm^2; the number of left leaf had the least effects on agronomic and economic characters of tobaccos. The research indicated that highly-qualified HQ No.1 can be produced, given that tobaccos are transplanted during March 10-March 15, planting density of 15 000-16 500 seedlings/hm^2, the number of left leaves of 22-24 leaves per seedling, and pure N of 150-165 kg/hm^2 in Hengyang. 展开更多
关键词 flue-cured tobaccos HQ No.1 Major cultivation technology Agronomic characters Economic character Tolerance to disease
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A new horizon for neuroscience:terahertz biotechnology in brain research 被引量:2
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作者 Zhengping Pu Yu Wu +2 位作者 Zhongjie Zhu Hongwei Zhao Donghong Cui 《Neural Regeneration Research》 SCIE CAS 2025年第2期309-325,共17页
Terahertz biotechnology has been increasingly applied in various biomedical fields and has especially shown great potential for application in brain sciences.In this article,we review the development of terahertz biot... Terahertz biotechnology has been increasingly applied in various biomedical fields and has especially shown great potential for application in brain sciences.In this article,we review the development of terahertz biotechnology and its applications in the field of neuropsychiatry.Available evidence indicates promising prospects for the use of terahertz spectroscopy and terahertz imaging techniques in the diagnosis of amyloid disease,cerebrovascular disease,glioma,psychiatric disease,traumatic brain injury,and myelin deficit.In vitro and animal experiments have also demonstrated the potential therapeutic value of terahertz technology in some neuropsychiatric diseases.Although the precise underlying mechanism of the interactions between terahertz electromagnetic waves and the biosystem is not yet fully understood,the research progress in this field shows great potential for biomedical noninvasive diagnostic and therapeutic applications.However,the biosafety of terahertz radiation requires further exploration regarding its two-sided efficacy in practical applications.This review demonstrates that terahertz biotechnology has the potential to be a promising method in the field of neuropsychiatry based on its unique advantages. 展开更多
关键词 biological effect brain NEURON NEUROPSYCHIATRY NEUROSCIENCE non-thermal effect terahertz imaging terahertz radiation terahertz spectroscopy terahertz technology
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Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum) 被引量:3
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作者 Xiu-ping LU Bing-guang XIAO +3 位作者 Yong-ping LI Yi-jie GUI Yu WANG Long-jiang FAN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第7期570-577,共8页
Diversity arrays technology (DART) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we g... Diversity arrays technology (DART) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which sug- gested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2-4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibil, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed. 展开更多
关键词 Nicofiana tabacum Diversity arrays technology (DART) Genetic diversity flue-cured tobacco
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Effects of Different Flue-curing Technologies on Microscopic Structure of Flue-cured Tobacco Leaves 被引量:1
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作者 Guomin CUI Bojun WANG +4 位作者 Rongchun LI Anding XU Yiyin CHEN Chao YANG Min PU 《Agricultural Biotechnology》 CAS 2014年第2期28-34,共7页
[Objective] This study aimed to compare the effects of Chinese quality-improving fragrunce-increasing tobacco flue-curing technology, Chinese three- stage tobaeeo flue-curing technology and Zimbabwean tobaceo flue-eur... [Objective] This study aimed to compare the effects of Chinese quality-improving fragrunce-increasing tobacco flue-curing technology, Chinese three- stage tobaeeo flue-curing technology and Zimbabwean tobaceo flue-euring technology on microscopie structure of flue-cured tobacco leaves to provide theoretical basis for tobacco flue-curing. [ Method ] Middle leaves of tobacco cultivar K326 were collected in Pengshui County of Chongqing City for flue-curing experiment using three flue-curing technologies. Leaf samples were collected regularly in the flue-curing process, to investigate the microscopic structure of flue-cured tobaceo leaves. [ Result] During three flue-curing processes, leaves, palisade tissues and sponge tissues shrank gradually. Three flue-euring processes exhibited significant differ- ences in the peak of tissue shrinkage : microscopic structure of flue-cured tobacco leaves in Chinese three-stage tobacco flue-curing process shrank earliest, follower by Chinese quality-improving fragrance-increasing tobacco flue-curing process; flue-eured tobacco leaves in Zimbabwean tobacco flue-curing process presented the latest shrinkage. At 84 h post-curing, leaf thickness in three flue-curing processes showed a descend order of Chinese three-stage tobacco flue-curing process 〉 Chinese quality-improving fragrance-increasing tobacco flue-curing process 〉 Zimbabwean tobacco flue-curing process. Upper and lower epidermal cells in Zimb- abwean tobacco flue-curing process ruptured earlier than other two flue-curing processes; eventually, the majority of cells ruptured and mixed with palisade tissues and sponge tissues. In Chinese quality-improving fragranee-increasing tobacco flue-curing process, only a small number of epidermal cells in dried leaves ruptured. Among three flue-curing technologies, Zimbabwean tobacco flue-curing technology exhibited the greatest damage to epidermal cells, followed by Chinese three-stage tobaceo flue-curing technology; Chinese quality-improving fragrance-increasing tobacco flue-curing technology had the minimum damage to epidermal cells. Mese- phyll cross-section exhibited significant morphological changes in stomata. To be specific, at 0 - 12 h post-curing, stomata of tobacco leaves closed with slightly prominent stamatal apparatuses on upper and lower epidermis; at 24 -72 h post-curing, stomata of tobaceo leaves changed gradually from opening to closure with significantly prominent stomatal apparatuses on upper and lower epidermis; at 84 h post-curing, stomata of tobacco leaves closed, and the majority of stomatal appa- ratuses were significantly prominent. In Chinese quality-improving fragrance-increasing tobacco flue-curing process, only a small number of epidermal ceils ruptured at 84 h pest-flue-curing; palisade tissues and sponge tissues shrank almost simultaneously. Significant gaps were observed between palisade tissues and between pal- isade tissues and sponge tissues. Chinese quality-improving fragrance-increasing tobacco flue-curing technology exhibited lower disorder level compared with other two flue-curing technologies. [ Conclusion] Chinese quality-improving fragrance-increasing tobacco flue-curlng technology was conducive to maintaining the micro- scopic structure integrity of flue-cured tobacco leaves and obtaining high-quality flue-cured tobacco leaves. 展开更多
关键词 flue-cured tobacco leaves flue-curing technologies Microscopic structure
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