Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modif...Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.展开更多
针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭...针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭州某处山区和纽约城市区域的高程模型;其次,结合地形高程信息,采用凸包策略编码并通过B样条曲线构建路径;最后,对樽海鞘群算法在个体位置更新公式上加入自适应Alpha稳定分布策略与非线性扰动策略,以平衡算法的全局开发能力与局部探索能力,并引入贪婪策略和鱼类聚集装置策略,提高算法搜索效率和精度。利用CEC2020测试函数对所提算法进行实验对比,验证了改进算法的性能。实验结果表明,凸包策略能有效提升算法规划能力,且与传统算法相比,改进后的算法能够使无人机的寻优精度更高,代价函数更小。展开更多
在民机自动飞行过程中,飞行导引系统的模式转换是影响安全的重要因素,应对其进行充分的安全性分析。传统安全分析方法主要关注各个组件的失效因素,忽略了由组件间非线性交互产生的安全问题。为此,采用系统理论过程分析(System Theory Pr...在民机自动飞行过程中,飞行导引系统的模式转换是影响安全的重要因素,应对其进行充分的安全性分析。传统安全分析方法主要关注各个组件的失效因素,忽略了由组件间非线性交互产生的安全问题。为此,采用系统理论过程分析(System Theory Process Analysis,STPA)方法,对飞行导引系统模式转换进行系统且完整的分析。同时,鉴于STPA方法中存在需人工分析的部分,引入了基于时间自动机理论的形式化模型检查工具UPPAAL对系统进行建模与验证,以确保控制结构图的正确性,并识别真正不安全控制行为(Unsafe Control Action,UCA),从而避免资源的浪费。最后,提出规范化的致因因素分析框架对通过验证的UCA进行逐一分析。实例证明,所提方法对航空类复杂系统安全性分析具有较好的效果。展开更多
为了减少荔枝汁在加热后产生的“蒸煮”异味,筛选了邻苯三酚、没食子酸、迷迭香酸、儿茶素、抗坏血酸及葡萄糖氧化酶六种抑制剂,从色泽、pH、香气轮廓、电子鼻、挥发性成分、降硫率以及总酚、总黄酮、抗氧化特性等角度去评价六种抑制剂...为了减少荔枝汁在加热后产生的“蒸煮”异味,筛选了邻苯三酚、没食子酸、迷迭香酸、儿茶素、抗坏血酸及葡萄糖氧化酶六种抑制剂,从色泽、pH、香气轮廓、电子鼻、挥发性成分、降硫率以及总酚、总黄酮、抗氧化特性等角度去评价六种抑制剂对荔枝汁风味及营养品质的影响。结果显示抗坏血酸和没食子酸抑制剂处理的样品与新鲜汁相比的色差值最小;整体香气轮廓显示葡萄糖氧化酶抑制蒸煮异味效果最佳,其次为迷迭香酸;电子鼻结果显示葡萄糖氧化酶处理的荔枝汁与新鲜汁最为接近,其次是儿茶素和邻苯三酚。挥发性成分的主成分分析结果显示抗坏血酸抑制剂处理的样品与新鲜汁最接近,其次为没食子酸、儿茶素、迷迭香酸。降硫率结果显示葡萄糖氧化酶的降硫效果最为显著,对二甲基硫醚、二甲基三硫醚、甲硫基丙醛的抑制率均达到100%,对二甲基二硫醚的抑制率为61.11%。营养品质方面,多酚类抑制剂与抗坏血酸处理的总酚、总黄酮含量和抗氧化性较新鲜汁均显著上升(P<0.05),而葡萄糖氧化酶处理的总酚、总黄酮含量较新鲜汁上升但抗氧化活性与新鲜汁无显著差异。综合色泽、pH、香气轮廓、营养品质以及降硫效果等因素进行偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA),发现葡萄糖氧化酶对荔枝汁整体品质影响最好。本研究可为荔枝以及其他热敏性水果加工过程中品质提升提供参考依据。展开更多
基金the National Natural Science Foundation of China,No. 30471934
文摘Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.
文摘针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭州某处山区和纽约城市区域的高程模型;其次,结合地形高程信息,采用凸包策略编码并通过B样条曲线构建路径;最后,对樽海鞘群算法在个体位置更新公式上加入自适应Alpha稳定分布策略与非线性扰动策略,以平衡算法的全局开发能力与局部探索能力,并引入贪婪策略和鱼类聚集装置策略,提高算法搜索效率和精度。利用CEC2020测试函数对所提算法进行实验对比,验证了改进算法的性能。实验结果表明,凸包策略能有效提升算法规划能力,且与传统算法相比,改进后的算法能够使无人机的寻优精度更高,代价函数更小。
文摘在民机自动飞行过程中,飞行导引系统的模式转换是影响安全的重要因素,应对其进行充分的安全性分析。传统安全分析方法主要关注各个组件的失效因素,忽略了由组件间非线性交互产生的安全问题。为此,采用系统理论过程分析(System Theory Process Analysis,STPA)方法,对飞行导引系统模式转换进行系统且完整的分析。同时,鉴于STPA方法中存在需人工分析的部分,引入了基于时间自动机理论的形式化模型检查工具UPPAAL对系统进行建模与验证,以确保控制结构图的正确性,并识别真正不安全控制行为(Unsafe Control Action,UCA),从而避免资源的浪费。最后,提出规范化的致因因素分析框架对通过验证的UCA进行逐一分析。实例证明,所提方法对航空类复杂系统安全性分析具有较好的效果。
文摘为了减少荔枝汁在加热后产生的“蒸煮”异味,筛选了邻苯三酚、没食子酸、迷迭香酸、儿茶素、抗坏血酸及葡萄糖氧化酶六种抑制剂,从色泽、pH、香气轮廓、电子鼻、挥发性成分、降硫率以及总酚、总黄酮、抗氧化特性等角度去评价六种抑制剂对荔枝汁风味及营养品质的影响。结果显示抗坏血酸和没食子酸抑制剂处理的样品与新鲜汁相比的色差值最小;整体香气轮廓显示葡萄糖氧化酶抑制蒸煮异味效果最佳,其次为迷迭香酸;电子鼻结果显示葡萄糖氧化酶处理的荔枝汁与新鲜汁最为接近,其次是儿茶素和邻苯三酚。挥发性成分的主成分分析结果显示抗坏血酸抑制剂处理的样品与新鲜汁最接近,其次为没食子酸、儿茶素、迷迭香酸。降硫率结果显示葡萄糖氧化酶的降硫效果最为显著,对二甲基硫醚、二甲基三硫醚、甲硫基丙醛的抑制率均达到100%,对二甲基二硫醚的抑制率为61.11%。营养品质方面,多酚类抑制剂与抗坏血酸处理的总酚、总黄酮含量和抗氧化性较新鲜汁均显著上升(P<0.05),而葡萄糖氧化酶处理的总酚、总黄酮含量较新鲜汁上升但抗氧化活性与新鲜汁无显著差异。综合色泽、pH、香气轮廓、营养品质以及降硫效果等因素进行偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA),发现葡萄糖氧化酶对荔枝汁整体品质影响最好。本研究可为荔枝以及其他热敏性水果加工过程中品质提升提供参考依据。
