Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modif...Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.展开更多
针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭...针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭州某处山区和纽约城市区域的高程模型;其次,结合地形高程信息,采用凸包策略编码并通过B样条曲线构建路径;最后,对樽海鞘群算法在个体位置更新公式上加入自适应Alpha稳定分布策略与非线性扰动策略,以平衡算法的全局开发能力与局部探索能力,并引入贪婪策略和鱼类聚集装置策略,提高算法搜索效率和精度。利用CEC2020测试函数对所提算法进行实验对比,验证了改进算法的性能。实验结果表明,凸包策略能有效提升算法规划能力,且与传统算法相比,改进后的算法能够使无人机的寻优精度更高,代价函数更小。展开更多
基金the National Natural Science Foundation of China,No. 30471934
文摘Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.
文摘针对启发式算法在无人机不规则复杂地形和多重威胁环境下进行三维航迹规划时,存在路径波动大和优化性能不足的问题,提出结合高程数据的凸包策略以及一种改进的樽海鞘群算法(ISSA)。首先,基于ASTER GDEMV3和Open Street Map数据,构建杭州某处山区和纽约城市区域的高程模型;其次,结合地形高程信息,采用凸包策略编码并通过B样条曲线构建路径;最后,对樽海鞘群算法在个体位置更新公式上加入自适应Alpha稳定分布策略与非线性扰动策略,以平衡算法的全局开发能力与局部探索能力,并引入贪婪策略和鱼类聚集装置策略,提高算法搜索效率和精度。利用CEC2020测试函数对所提算法进行实验对比,验证了改进算法的性能。实验结果表明,凸包策略能有效提升算法规划能力,且与传统算法相比,改进后的算法能够使无人机的寻优精度更高,代价函数更小。
