目的利用线粒体细胞色素c氧化酶亚基Ⅰ(cytochrome c oxidase subunitⅠ,COⅠ)基因序列对长三角地区常见嗜尸性蝇类物种进行分子鉴定并验证其可靠性。方法于野外放置的大型家猪尸体上采集并经实验室多代培养、遗传稳定的长三角地区常见...目的利用线粒体细胞色素c氧化酶亚基Ⅰ(cytochrome c oxidase subunitⅠ,COⅠ)基因序列对长三角地区常见嗜尸性蝇类物种进行分子鉴定并验证其可靠性。方法于野外放置的大型家猪尸体上采集并经实验室多代培养、遗传稳定的长三角地区常见嗜尸性蝇类共3科6属7个物种,提取全基因组DNA后扩增COⅠ基因片段,正反向测序后拼接,通过Mega 7.0.26等软件分析扩增片段的碱基组成和种间进化分歧率,并采用邻接(neighbor joining,NJ)法和非加权组平均法(unweighted pair-group method with arithmetic means,UPGMA)构建长三角地区常见嗜尸性蝇类COⅠ基因序列的系统发育树。结果不同蝇类物种的平均碱基组成为A(30.14%)、T(38.23%)、C(15.98%)、G(15.65%)。种间进化分歧率在2.2%~15.3%,最低在大头金蝇和肥躯金蝇之间,最高在厩腐蝇和棕尾别麻蝇之间。结论利用COⅠ基因能够较好地鉴别长三角地区的常见嗜尸性蝇类物种。展开更多
There have been increasing efforts to utilize energy by-products （EBP） all over the world. In the Czech Re- public fly ash is usually used in ceramic technology, es- pecially in brick manufacturing and for ceramic t...There have been increasing efforts to utilize energy by-products （EBP） all over the world. In the Czech Re- public fly ash is usually used in ceramic technology, es- pecially in brick manufacturing and for ceramic tiles. The average production of EBP is about Ig million tons per year. The range of potential products, where EBP could be used, is very wide and energy by-products have become an important raw material source. In this paper the attention was focused on class C fly ash and its usage in field of refractory materials. Experimental works were carried out on mixtures with fly ash and clay. There were also tested batches for lightweight fireclay bricks. The maximal amount of CFA should be up to 50%.展开更多
文摘There have been increasing efforts to utilize energy by-products （EBP） all over the world. In the Czech Re- public fly ash is usually used in ceramic technology, es- pecially in brick manufacturing and for ceramic tiles. The average production of EBP is about Ig million tons per year. The range of potential products, where EBP could be used, is very wide and energy by-products have become an important raw material source. In this paper the attention was focused on class C fly ash and its usage in field of refractory materials. Experimental works were carried out on mixtures with fly ash and clay. There were also tested batches for lightweight fireclay bricks. The maximal amount of CFA should be up to 50%.
