Quantitative Structure-Activity Relationship (2D-QSAR) models for binding affinity constants (log Ki) of 78 flavanoid ligands towards the benzodiazepine site of GABA (A) receptor complex were estimated using the PRECL...Quantitative Structure-Activity Relationship (2D-QSAR) models for binding affinity constants (log Ki) of 78 flavanoid ligands towards the benzodiazepine site of GABA (A) receptor complex were estimated using the PRECLAV (Property-Evaluation by Class Variables) program. The best MLR equation with nine PRECLAV descriptors has R2 = 0.843 and R2C = 0.782. Attempt is also made for obtaining 2D-QSAR model using NCSS software. The comparison of the results indicated that the PRECLAV method is very efficient in detecting structure-activity correlation with good predictive power.展开更多
以牡丹叶为原料,采用酶法提取牡丹叶黄酮,以纤维素酶用量、p H值、酶解温度、酶解时间为单因素,以牡丹叶黄酮提取率为考察指标,通过正交试验确定酶法提取牡丹叶黄酮的最佳工艺参数为:纤维素酶用量12.5 U/m L、p H 4.5、酶解温度45℃、...以牡丹叶为原料,采用酶法提取牡丹叶黄酮,以纤维素酶用量、p H值、酶解温度、酶解时间为单因素,以牡丹叶黄酮提取率为考察指标,通过正交试验确定酶法提取牡丹叶黄酮的最佳工艺参数为:纤维素酶用量12.5 U/m L、p H 4.5、酶解温度45℃、酶解时间4 h。在此条件下牡丹叶黄酮提取率为2.43%。提取得到的牡丹叶黄酮浸提液可用于亚硝酸盐清除,通过正交试验优化得到牡丹叶黄酮清除亚硝酸盐的最佳反应条件为:反应温度70℃、p H 4.0、牡丹叶黄酮提取液添加量25 m L(10μg亚硝酸钠)、反应时间20 min。在此条件下牡丹叶黄酮对亚硝酸盐清除率可达62.15%。展开更多
文摘Quantitative Structure-Activity Relationship (2D-QSAR) models for binding affinity constants (log Ki) of 78 flavanoid ligands towards the benzodiazepine site of GABA (A) receptor complex were estimated using the PRECLAV (Property-Evaluation by Class Variables) program. The best MLR equation with nine PRECLAV descriptors has R2 = 0.843 and R2C = 0.782. Attempt is also made for obtaining 2D-QSAR model using NCSS software. The comparison of the results indicated that the PRECLAV method is very efficient in detecting structure-activity correlation with good predictive power.
文摘以牡丹叶为原料,采用酶法提取牡丹叶黄酮,以纤维素酶用量、p H值、酶解温度、酶解时间为单因素,以牡丹叶黄酮提取率为考察指标,通过正交试验确定酶法提取牡丹叶黄酮的最佳工艺参数为:纤维素酶用量12.5 U/m L、p H 4.5、酶解温度45℃、酶解时间4 h。在此条件下牡丹叶黄酮提取率为2.43%。提取得到的牡丹叶黄酮浸提液可用于亚硝酸盐清除,通过正交试验优化得到牡丹叶黄酮清除亚硝酸盐的最佳反应条件为:反应温度70℃、p H 4.0、牡丹叶黄酮提取液添加量25 m L(10μg亚硝酸钠)、反应时间20 min。在此条件下牡丹叶黄酮对亚硝酸盐清除率可达62.15%。
