Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined b...Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.展开更多
基金supported by the International Scientific and Technological Cooperation Program of China(No:2009DFA31230)the Industry-University-Research Cooperation Program from Science and Technology Department of Qingyuang City(No:2012D021211001)
文摘Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.
