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Evaluation and Exploration of Citrus Germplasm Resources in the Origin of Shatangju(Citrus flamea Hort.ex Tseng Shiyueju)
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作者 Yanjun GUO Qianhua JI +6 位作者 Liying GUO Guochan HUANG Bingwa FANG Liujian HUANG Hui JIANG Fengmei YANG Yaping HU 《Asian Agricultural Research》 2023年第10期28-33,共6页
[Objectives]To protect the local rare citrus germplasm resources and further develop and cultivate new citrus varieties.[Methods]The germplasm resources of Shatangju(Citrus flamea Hort.Ex Tseng Shiyueju)in Sihui City ... [Objectives]To protect the local rare citrus germplasm resources and further develop and cultivate new citrus varieties.[Methods]The germplasm resources of Shatangju(Citrus flamea Hort.Ex Tseng Shiyueju)in Sihui City of Guangdong Province and other 8 citrus varieties from the same origin were evaluated.The appearance characteristics of tree shape,the length and thickness of shoot at each shoot stage,the appearance characteristics of leaves and the fruit quality of each variety were investigated.[Results]Through the comparison of various citrus varieties grafted with sour mandarin,the results showed that the overall advantages of the length and thickness of the shoots of Shatang tangerine in summer and autumn were more prominent,which was conducive to fruit setting.The sprouting time of the three shoots of each variety was different,and there were sporadic sprouting phenomena in different seasons,so the growth was different,and the length and thickness of the shoots were different in different seasons.There were significant differences in shoot length and diameter among different varieties in the same season.The coefficient of variation of Shatangju's leaves was low,and the traits were stable;leaf shape,leaf base shape,leaf margin and other indicators among the three shoots of the same variety and the differences among the same shoots of different varieties were compared.All varieties had strong flavor,separately sweet and sour,sweet and sweet taste.Shatangju has many segments,orange pericarp,rough texture,easy peeling,and high oblate fruit shape.The orange pericarp was favored by the participants,while the rough and thick peel was not favored by the participants.[Conclusions]Based on the improvement of local varieties,it is recommended to improve the smoothness of pericarp,reducing the thickness of pericarp and improving the flavor of fruit will be conducive to increasing sales.In the cultivation process of various quality resources,especially in the period of fruit growth and development,the plan of nutrition and fertilization should be formulated according to the rules of fruit growth and development,the rules of yield formation and the rules of quality composition,so as to establish the integrated nutrient management system of various varieties. 展开更多
关键词 Shatangju(Citrus flamea Hort.ex Tseng Shiyueju) Germplasm resources EVALUATION Zhaoqing City
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砂糖桔全长cDNA文库的构建及病程相关蛋白基因的克隆与分析
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作者 胡亚平 郭雁君 +4 位作者 吉前华 蒋惠 郭丽英 张小凤 周希琴 《广东农业科学》 CAS 2015年第16期89-95,F0003,共8页
为从分子水平上了解影响砂糖桔生理代谢、生长发育、病虫害抗性和品质的众多基因,以无籽砂糖桔幼叶为材料,应用SMART技术构建了cDNA文库,检测文库发现其库容量达到1.36×10^6,重组率为95%,插入片段绝大多数分布在0.6~2kb... 为从分子水平上了解影响砂糖桔生理代谢、生长发育、病虫害抗性和品质的众多基因,以无籽砂糖桔幼叶为材料,应用SMART技术构建了cDNA文库,检测文库发现其库容量达到1.36×10^6,重组率为95%,插入片段绝大多数分布在0.6~2kb之间,有36%的插入片段是全长cDNA。随机挑选224个克隆进行测序,获得了1个病程相关蛋白基因的全长cDNA序列。将基因序列提交GenBank,编号为KJ000019。基因的CDS长429bp,5’UTR为43bp,3’UTR为235bp,编码长度为143个氨基酸残基的蛋白。基因位于第8染色体上,有1个长度为193bp的内含子,基因编码的蛋白质分子量为15.4ku,等电点为6.96。该蛋白N端含有长度约为22aa的信号肽序列,指导蛋白分泌到细胞外;剩余部分是一个典型的Barwin保守结构域,具有几丁质酶的活性,在受到细菌、真菌侵染时可以分解部分真菌和细菌的细胞壁,发挥抗病作用。 展开更多
关键词 砂糖桔 CDNA文库 病程相关蛋白
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基于SRAP分子标记的11份柑橘种质材料遗传多样性分析 被引量:6
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作者 燕佳文 武晓晓 +2 位作者 唐艳 陈传武 邓崇岭 《分子植物育种》 CAS 北大核心 2021年第2期664-671,共8页
为分析11份柑橘种质材料的遗传多样性,本研究以7份沙糖橘种质以及4份其他柑橘种质为试验材料,利用SRAP分子标记进行分析,并用UPGMA法进行聚类。结果表明:160对引物组合筛选出20对引物组合扩增条带清晰,11份种质材料两两之间遗传相似系数... 为分析11份柑橘种质材料的遗传多样性,本研究以7份沙糖橘种质以及4份其他柑橘种质为试验材料,利用SRAP分子标记进行分析,并用UPGMA法进行聚类。结果表明:160对引物组合筛选出20对引物组合扩增条带清晰,11份种质材料两两之间遗传相似系数在0.322 1~0.931 6之间。沙糖橘和金葵蜜橘间的遗传相似系数最大,为0.931 6;姑婆山野生元橘和宫川间的遗传相似系数最小,为0.322 1。聚类结果显示,在相似系数为0.861 9时,早熟沙糖橘、沙糖橘、金葵蜜橘、灯笼沙糖橘、八月橘聚为一组,在0.810 8时与红皮酸橘聚为一组,继而再与别的柑橘品种聚为一组。金葵蜜橘与沙糖橘间不同引物扩增的电泳条带存在特异性,证实了金葵蜜橘是沙糖橘的芽变品种。灯笼沙糖橘是沙糖橘变异新类型,二者间遗传相似系数为0.921 1,不同引物扩增结果存在特异性条带,证实了灯笼沙糖橘是沙糖橘的芽变新类型,可以登记为新品种;根据遗传相似系数、聚类分析、不同引物扩增产物的电泳结果推测沙糖蜜柑是沙糖橘和温州蜜柑的自然杂交后代,可以登记为新品种;姑婆山野生元橘与其它柑橘材料亲缘关系较远,也佐证了姑婆山野生元橘比道县野橘更为原始,是一个独特的、原始的宽皮柑橘类型;在7份沙糖橘材料及4份其它柑橘材料中,沙糖橘与红皮酸橘亲缘关系最近,二者在遗传相似系数0.822 5处聚为一类,由此推测沙糖橘有可能由红皮酸橘进化而来。目前关于沙糖橘遗传多样性方面的研究较少,本研究结果可为沙糖橘的遗传多样性以及不同沙糖橘品种的来源和亲缘关系研究提供理论依据。 展开更多
关键词 沙糖橘 SRAP分子标记 遗传多样性 聚类分析
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