The unique fruity aroma of strawberries enhances their appeal to consumers.Fragaria nilgerrensis,a wild strawberry distributed in Southwest China and known for its distinctive peach-like aroma,represents a valuable re...The unique fruity aroma of strawberries enhances their appeal to consumers.Fragaria nilgerrensis,a wild strawberry distributed in Southwest China and known for its distinctive peach-like aroma,represents a valuable resource for improving the fragrance of cultivated strawberries.However,the molecular mechanism underlying the peach-like aroma biosynthesis remains largely unexplored.In this study,we integrated metabolomic and transcriptomic data across four developmental stages of F.nilgerrensis fruits to construct a detailed profile of volatile organic compounds(VOCs)and the associated gene expression alterations during fruit maturation.Our findings reveal that g-decalactone,d-decalactone,and g-undecalactone are the primary compounds responsible for the pronounced peach-like aroma,with their levels showing a significant correlation with the activity of the FngFAD2 enzyme.The silencing of the FngFAD2 gene through tobacco rattle viral(TRV)induction resulted in notable reductions in both the peach-like aroma and lactone content in the fruit.In addition,integrating dual luciferase assays,yeast one-hybrid(Y1H)and subcellular localization experiments,we also identified three transcription factors(FngDOF1.2,FngWRKY3,and FngWRI1)that enhance FngFAD2 expression.These findings elucidate the molecular regulatory network involved in the complex developmental process of peach-like flavor in strawberry fruits.Additionally,our research also provides a foundation for the utilization of the wild strawberry as well as improving the flavor and quality of cultivated strawberries.展开更多
Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessi...Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessitates post-harvest treatment to extend their shelf life and preserve their quality,as strawberries are known for their fragile skin and soft texture.To fully utilize konjac and develop high-quality coatingfilms,native konjacflour(NKF)and konjac glucomannan(KGM)were extracted from its corm and used as a coatingfilm for strawberries in the present study.Therefore,this study aimed to compare the physical properties of thefilm coatings between NKF and KGM,and evaluate their effects on strawberries preservation over 7 days of storage.A multistage extrac-tion process was employed to isolate NKF and KGM,after which the glucomannan content was measured.NKF yield was 31.81%,exceeding KGM yield of 26.42%,and the glucomannan content obtained of NKF(25.93%)was higher than KGM(21.41%).Nuclear magnetic resonance spectroscopy confirmed that both NKF and KGM con-tain glucomannan in their structure.Furthermore,both NKF and KGM were combined with carboxymethyl cel-lulose(CMC)and glycerol to produce eight thin-layerfilms to assess their physical and mechanical properties.Compared to the KGM variant,the NKF variant generally exhibited higher moisture content,water vapor trans-mission rate,and tensile strength.However,NKF was less effective than KGM in extending strawberry storage life,leading to faster color changes and greater weight loss,despite maintaining similar hardness values.Nonetheless,konjac-based coatings were generally effective at maintaining the freshness and quality of strawberries compared to uncoated samples.Konjac shows promise as an edible coating,improving fresh produce shelf life and appeal,aligning with consumer preferences for natural and sustainable products.展开更多
Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,an...Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,and less competitive individual farmers,struggle to meet the compliance and scalability demands of E-commerce,thereby constraining high-quality local economic development.Aiming to address this issue,this paper,guided by relevant policies and strategies,employs case analysis and logical deduction to explore the industrialization path of the"Cooperative+E-commerce"model for the strawberry industry.The research finds that by optimizing the cooperative's organizational structure,implementing multi-channel E-commerce strategies,upgrading the supply chain(including cold chain and quality traceability),and engaging in collaborative brand building,a robust industrial system can be formed.Supplemented by benefit evaluation,policy support,and regulatory oversight,this system can effectively bridge small-scale production with the broader market.This study concludes that this pathway can enhance the added value of the strawberry industry,increase farmer incomes,and provide practical insights for promoting high-quality local economic development.展开更多
Strawberry (Fragaria ananassa) is well known among consumers because of its attractive color, delicious taste, and nutritional benefits. It is widely grown worldwide, but its production has become a significant challe...Strawberry (Fragaria ananassa) is well known among consumers because of its attractive color, delicious taste, and nutritional benefits. It is widely grown worldwide, but its production has become a significant challenge due to changing climatic conditions that lead to abiotic stresses in plants, which results in poor root development, nutrient deficiency, and poor plant health. In this context, the major abiotic stresses are temperature fluctuations, water shortages, and high levels of soil salinity. The accumulation of salts in excessive amounts disrupts the osmotic balance and impairs physiological processes. However, drought reduces fruit size, yield, and quality. Similarly, heat and cold stresses directly affect the rate of photosynthesis. Plants respond to these changes by producing growth-promoting hormones to ensure their survival. In the context of these abiotic stresses, beneficial microbes support plant growth. Among these fungi, the most extensively studied are plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF). When applied as bioinoculants, they are associated with roots and subsequently improve soil health, fruit quality, and overall crop yield. This review highlights the impacts of abiotic stresses on strawberry roots, growth, and hormonal pathways. Moreover, it focuses on the role of beneficial soil microbes in the mitigation of these responses.展开更多
Anthocyanins are the flavonoid pigments responsible for vibrant fruit and flower colors,and they also play key roles in both plant physiology and human health.MYB transcription factors are crucial regulators of anthoc...Anthocyanins are the flavonoid pigments responsible for vibrant fruit and flower colors,and they also play key roles in both plant physiology and human health.MYB transcription factors are crucial regulators of anthocyanin biosynthesis and accumulation,but the functional differences of homologous MYB transcription factors in regulating anthocyanin content are still unclear.In strawberry(Fragaria×ananassa),FaMYB44.1 and FaMYB44.3 are highly homologous MYB transcription factors localized in the nucleus and can be significantly induced by weak light.However,they differ in their effects on anthocyanin accumulation in the fruits.FaMYB44.1 inhibits anthocyanin synthesis by transcriptionally suppressing FaF3H,which is essential for anthocyanin regulation,in the‘BeniHoppe'and‘JianDe-Hong'strawberry varieties.In contrast,FaMYB44.3 does not affect anthocyanin levels.This study provides a comprehensive overview of the roles of FaMYB44.1 and FaMYB44.3 in anthocyanin regulation in strawberry fruits.By elucidating the molecular mechanisms underlying their regulation,this study enhances our understanding of how the interactions between genetic and environmental factors control fruit pigmentation and enhance the nutritional value of the fruit.展开更多
[Objective] The objective of this study was to investigate the effects of different agricultural managements on soil microbial population,activity,functional diversity and soil enzyme activity in continuous mono-cropp...[Objective] The objective of this study was to investigate the effects of different agricultural managements on soil microbial population,activity,functional diversity and soil enzyme activity in continuous mono-cropping field of strawberry.[Method]A field plot experiment was carried out to evaluate characteristics of soil microbial community by soil enzyme analysis,microbial cultivation and Biolog analysis.[Result]The results showed that bacteria population proportion,the ration of bacteria to fungi,microorganism amount,AWCD,soil dehydrogenase activity,the Shannon,Simpson,and Mcintosh indices of soil microbial communities were obviously increased under strawberry-rice rotation,soil solarization with rice bran,and calcium cyanamide（CaCN2）treatments,in addition,soil urease activity was significantly increased under strawberry-rice rotation and soil solarization with rice bran treatment,when compared with no fertilization.When compared with conventional fertilization treatment,strawberry-rice rotation and soil solarization with rice bran both significantly increased AWCD and Mcintosh index of soil microbial communities,meanwhile respectively increased soil urease and dehydrogenase activity.PCA analyses suggested that carbon utilization of soil microbial communities under strawberry-rice rotation,soil solarization with rice bran,and calcium cyanamide treatment was obviously different from that of conventional fertilization and no fertilization treatment.[Conclusion] strawberry-rice rotation and soil solarization with rice bran were effective agricultural managements to control soil biological degradation under Continuous Cropped Strawberry.展开更多
[Objective] The aim was to establish a set of soilless cultivation and management method of facility strawberry cultivation in the south of China.[Method] In the research,strawberry species,substrate formulae,cultivat...[Objective] The aim was to establish a set of soilless cultivation and management method of facility strawberry cultivation in the south of China.[Method] In the research,strawberry species,substrate formulae,cultivation modes and nutri ent solutions were selected and optimized.[Result] Three-dimensional strawberry cultivation was much suitable for Frandy,Hongbao and Gumei No.1; substrate with coconut shred-mushroom residue-peat soil ratio at 2∶2∶1 was the optimal; the treatment C3,containing Ca（NO3）2·4H2O,KNO3,（NH4）2HPO4,MgSO4·7H2O,and NH4NO3 at 280,303,62,246 and 80 mg/L,promoted strawberry growth,and the cultivated strawberry was large and thick in leaves,thick in stem,dark green in leaf color,with best fruit yield and quality.Among three cultivation modes,the density,space use ratio and yield in the treatment with PVC pipes in A-shape were all higher and the strawberry was easier and more convenient for picking.[Conclusion] The research provides references for soilless cultivation of strawberry in the south of China.展开更多
[Objective] This study aimed to investigate the effect of different boric fer- tilizer on the growth, yield and quality of strawberry. [Method] With a strawberry cultivar Hongyan as the test material, water, borax and...[Objective] This study aimed to investigate the effect of different boric fer- tilizer on the growth, yield and quality of strawberry. [Method] With a strawberry cultivar Hongyan as the test material, water, borax and foliar disodium octoborate tetrahydrate was respectively sprayed to the leaves of strawberry four times from the squaring stage on. At the initial flowering stage and mature stage, the plant height, stem diameter, leaf area and petiole length of strawberry were measured; at the harvest time, the single fruit weight of strawberry was measured; and at the mature stage, the VC, sugar and organic acids contents in the strawberry fruit were determined. [Result] Compared with the control group, the spraying of foliar disodi- um octoborate tetrahydrate promoted the growth and significantly increased the plant height and leaf area of strawberry. However, no significant difference was found in root shoot ratio of strawberry between foliar disodium octoborate tetrahydrate treat- ment and the control group. Spraying of boric fertilizers significantly increased the single fruit weight and yield of strawberry. Compare with the control group, the spraying of foliar disodium octoborate tetrahydrate and borax increased the single fruit weight of strawberry by 20.14% and 4.86% respectively, and increased the yield of strawberry by 17.28% and 4.02% respectively. Compared with borax treat- ment and the control group, spraying of foliar disodium octoborate tetrahydrate im- proved the quality, i.e., increased the VC content, soluble solids content and sugar- acid ratio in strawberry fruit. The B content in leaf of strawberry was increased after foliar B fertilization. Among the three groups, the B content in leaf of strawberry ranked as disodium octoborate tetrahydrate treatment＇s 〉 borax treatment＇s 〉 con- trol＇s. [Conclusion] Spraying foliar disodium octoborate tetrahydrate can promote the growth, yield and quality of strawberry.展开更多
[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed b...[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.展开更多
This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extens...This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extensively planted but not resistant to anthrax. The reciprocal crosses were performed using Benihoppe, as the female parent, four disease-resistant European cultivars Albion, Camino Real, Ven- tana and Virginia, and one Japanese cultivar Meiho as the male parent. Then, the seedling propagation coefficient, disease resistance, fruit quality and yield of F1 gen- eration of the combinations were detected and compared. Besides, the breeding and traits of a new cultivar F1-40 developed from the cross between Benihoppe and Mei- ho were introduced as an instance to prove the feasibility of the combinations/lines.展开更多
This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. Acc...This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. According to the results of sin-gle factor test, an orthogonal experiment was designed to determine the optimum extraction conditions. The effects of the four factors on anthocyanins extraction from strawberry were listed here in an decreasing order： ethanol concentration 〉 solid-liq-uid ratio〉duration of ultrasonic treatment〉ultrasonic power. And the optimal condi-tions of ultrasonic extraction of anthocyanins from strawberry were： ultrasonic treat-ment of 30 min, solid-liquid ratio of 1：15, ethanol concentration of 60% and ultra-sonic power of 300 W. Under such conditions, the content of extracted antho-cyanins was 33.247 mg/100g, which was 1.3 folds higher than that by extraction without ultrasonic treatment.展开更多
[Objective] The aim was to investigate the application of plant growth regulator thidiazuron（TDZ）in strawberry anther culture.[Method]Different combinations of TDZ and naphthalene acetic acid（NAA）auxine with diffe...[Objective] The aim was to investigate the application of plant growth regulator thidiazuron（TDZ）in strawberry anther culture.[Method]Different combinations of TDZ and naphthalene acetic acid（NAA）auxine with different concentrations were used for induction culture,and then its effect on callus induction and plant regeneration were observed.[Result] The combination of 1.0 mg/L TDZ＋0.5 mg/LNAA was effective to accelerating the differentiation and regeneration of strawberries,and the differentiation ratio was as high as 75%.However,TDZ showed insignificant effect on strawberries anther induction.[Conclusion]This research had provided reference for large-scale production of strawberry through anther culture.展开更多
It is proved that the treatment with white mulch and black thin films per- formed the best in terms of heat preservation in cultivation of strawberry with shelves. In winter, drip irrigation performed significantly in...It is proved that the treatment with white mulch and black thin films per- formed the best in terms of heat preservation in cultivation of strawberry with shelves. In winter, drip irrigation performed significantly in transporting hot water through solar energy. The combination of the two methods resolved the issue of heat preservation difficulty and guaranteed growth of strawberry in winter.展开更多
[Objective] This study aimed to identify strawberry anthracnose pathogens and screen appropriate germicides. [Method] Strawberry anthracnose pathogens in Jurong City of Jiangsu Province were isolated and identified. T...[Objective] This study aimed to identify strawberry anthracnose pathogens and screen appropriate germicides. [Method] Strawberry anthracnose pathogens in Jurong City of Jiangsu Province were isolated and identified. The toxicities of sever-al germicides to Col etotrichum fragariae were determined with indoor mycelial growth inhibition assay. Field control test was preformed to further verify the control efficacy of germicides. [Result] The isolated strawberry anthracnose pathogenic strains were identified as C. gloeosporioide from genus Col etotrichum of subphylum Deuteromycotina. Prochloraz, pyrazole Kresoxim and hexaconazole al exhibited good indoor control effects; 25% pyrazole Kresoxim EC2000-fold solution, 24% azoxys-trobin＆#183;hexaconazole （azoxystrobin∶hexaconazole=1∶2） SC1500-fold solution and 25%prochloraz EC1000-fold solution al exhibited significant field control efficacy. [Con-clusion] The study provides reference for the alternate use of germicides in actual production.展开更多
From middle December, 2012 to early January, 2013, Jinan City was in- fluenced by cold airs and successive rainy and snowy days lasted for 13 d. Consequently, strawberry in greenhouse was seriously affected. In the re...From middle December, 2012 to early January, 2013, Jinan City was in- fluenced by cold airs and successive rainy and snowy days lasted for 13 d. Consequently, strawberry in greenhouse was seriously affected. In the research, the causes of strawberry cold injury were surveyed and analyzed, to provide references for scientific management for farmers.展开更多
In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote t...In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.展开更多
基金supported by grants from National Natural Science Foundation of China(Grant Nos.32372655,32060085,32260094,32060237)Applied Basic Research Project of Yunnan(Grant Nos.202301AS070071,and 202101AZ070001-166)Yunnan Innovation Guidance and Technology Enterprise Cultivation Plan Project(Grant No.202304BT090032).
文摘The unique fruity aroma of strawberries enhances their appeal to consumers.Fragaria nilgerrensis,a wild strawberry distributed in Southwest China and known for its distinctive peach-like aroma,represents a valuable resource for improving the fragrance of cultivated strawberries.However,the molecular mechanism underlying the peach-like aroma biosynthesis remains largely unexplored.In this study,we integrated metabolomic and transcriptomic data across four developmental stages of F.nilgerrensis fruits to construct a detailed profile of volatile organic compounds(VOCs)and the associated gene expression alterations during fruit maturation.Our findings reveal that g-decalactone,d-decalactone,and g-undecalactone are the primary compounds responsible for the pronounced peach-like aroma,with their levels showing a significant correlation with the activity of the FngFAD2 enzyme.The silencing of the FngFAD2 gene through tobacco rattle viral(TRV)induction resulted in notable reductions in both the peach-like aroma and lactone content in the fruit.In addition,integrating dual luciferase assays,yeast one-hybrid(Y1H)and subcellular localization experiments,we also identified three transcription factors(FngDOF1.2,FngWRKY3,and FngWRI1)that enhance FngFAD2 expression.These findings elucidate the molecular regulatory network involved in the complex developmental process of peach-like flavor in strawberry fruits.Additionally,our research also provides a foundation for the utilization of the wild strawberry as well as improving the flavor and quality of cultivated strawberries.
基金funded by the Academic Leadership Grant of Universitas Padjadjaran,Bandung,Indonesia,with grant number 1540/UN6.3.1/PT.00/2024the Research Collaboration Center for Biomass and Biorefinery,Bandung,Indonesia,with grant number B-1723/II.7/HK.01.00/4/2024.
文摘Konjac is an ideal candidate for edible coatings on fruits due to its hydrophilic properties,film-forming ability,barrier properties,safety,and biodegradability.Meanwhile,the high market demand for strawberries necessitates post-harvest treatment to extend their shelf life and preserve their quality,as strawberries are known for their fragile skin and soft texture.To fully utilize konjac and develop high-quality coatingfilms,native konjacflour(NKF)and konjac glucomannan(KGM)were extracted from its corm and used as a coatingfilm for strawberries in the present study.Therefore,this study aimed to compare the physical properties of thefilm coatings between NKF and KGM,and evaluate their effects on strawberries preservation over 7 days of storage.A multistage extrac-tion process was employed to isolate NKF and KGM,after which the glucomannan content was measured.NKF yield was 31.81%,exceeding KGM yield of 26.42%,and the glucomannan content obtained of NKF(25.93%)was higher than KGM(21.41%).Nuclear magnetic resonance spectroscopy confirmed that both NKF and KGM con-tain glucomannan in their structure.Furthermore,both NKF and KGM were combined with carboxymethyl cel-lulose(CMC)and glycerol to produce eight thin-layerfilms to assess their physical and mechanical properties.Compared to the KGM variant,the NKF variant generally exhibited higher moisture content,water vapor trans-mission rate,and tensile strength.However,NKF was less effective than KGM in extending strawberry storage life,leading to faster color changes and greater weight loss,despite maintaining similar hardness values.Nonetheless,konjac-based coatings were generally effective at maintaining the freshness and quality of strawberries compared to uncoated samples.Konjac shows promise as an edible coating,improving fresh produce shelf life and appeal,aligning with consumer preferences for natural and sustainable products.
基金General Project of Philosophy and Social Sciences Research in Universities of Jiangsu Province,2024(2024SJYB1650).
文摘Under the background of"Digital Commerce for Rural Vitalization",rural E-commerce has experienced rapid development.However,agricultural products like strawberries,often produced by small-scale,fragmented,and less competitive individual farmers,struggle to meet the compliance and scalability demands of E-commerce,thereby constraining high-quality local economic development.Aiming to address this issue,this paper,guided by relevant policies and strategies,employs case analysis and logical deduction to explore the industrialization path of the"Cooperative+E-commerce"model for the strawberry industry.The research finds that by optimizing the cooperative's organizational structure,implementing multi-channel E-commerce strategies,upgrading the supply chain(including cold chain and quality traceability),and engaging in collaborative brand building,a robust industrial system can be formed.Supplemented by benefit evaluation,policy support,and regulatory oversight,this system can effectively bridge small-scale production with the broader market.This study concludes that this pathway can enhance the added value of the strawberry industry,increase farmer incomes,and provide practical insights for promoting high-quality local economic development.
文摘Strawberry (Fragaria ananassa) is well known among consumers because of its attractive color, delicious taste, and nutritional benefits. It is widely grown worldwide, but its production has become a significant challenge due to changing climatic conditions that lead to abiotic stresses in plants, which results in poor root development, nutrient deficiency, and poor plant health. In this context, the major abiotic stresses are temperature fluctuations, water shortages, and high levels of soil salinity. The accumulation of salts in excessive amounts disrupts the osmotic balance and impairs physiological processes. However, drought reduces fruit size, yield, and quality. Similarly, heat and cold stresses directly affect the rate of photosynthesis. Plants respond to these changes by producing growth-promoting hormones to ensure their survival. In the context of these abiotic stresses, beneficial microbes support plant growth. Among these fungi, the most extensively studied are plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF). When applied as bioinoculants, they are associated with roots and subsequently improve soil health, fruit quality, and overall crop yield. This review highlights the impacts of abiotic stresses on strawberry roots, growth, and hormonal pathways. Moreover, it focuses on the role of beneficial soil microbes in the mitigation of these responses.
基金sponsored by the Zhongshan Biological Breeding Laboratory Grant,China(ZSBBL-KY2023-08)the Natural Science Foundation of Jiangsu Province,China(BK20230572)the Basic Sciences(Natural Sciences)Research Project in Universities of Jiangsu Province,China(23KJB210015)。
文摘Anthocyanins are the flavonoid pigments responsible for vibrant fruit and flower colors,and they also play key roles in both plant physiology and human health.MYB transcription factors are crucial regulators of anthocyanin biosynthesis and accumulation,but the functional differences of homologous MYB transcription factors in regulating anthocyanin content are still unclear.In strawberry(Fragaria×ananassa),FaMYB44.1 and FaMYB44.3 are highly homologous MYB transcription factors localized in the nucleus and can be significantly induced by weak light.However,they differ in their effects on anthocyanin accumulation in the fruits.FaMYB44.1 inhibits anthocyanin synthesis by transcriptionally suppressing FaF3H,which is essential for anthocyanin regulation,in the‘BeniHoppe'and‘JianDe-Hong'strawberry varieties.In contrast,FaMYB44.3 does not affect anthocyanin levels.This study provides a comprehensive overview of the roles of FaMYB44.1 and FaMYB44.3 in anthocyanin regulation in strawberry fruits.By elucidating the molecular mechanisms underlying their regulation,this study enhances our understanding of how the interactions between genetic and environmental factors control fruit pigmentation and enhance the nutritional value of the fruit.
基金Supported by the National Science and Technology Funds for Agriculture(2009GB24910540)Special fund for National Public Service Sectors(Agriculture)Research(200903011)+1 种基金Natural Science Fund Project of Hohai University(2008429811)Central University Basic Research Operating Expenses Project(2010B05314)~~
文摘[Objective] The objective of this study was to investigate the effects of different agricultural managements on soil microbial population,activity,functional diversity and soil enzyme activity in continuous mono-cropping field of strawberry.[Method]A field plot experiment was carried out to evaluate characteristics of soil microbial community by soil enzyme analysis,microbial cultivation and Biolog analysis.[Result]The results showed that bacteria population proportion,the ration of bacteria to fungi,microorganism amount,AWCD,soil dehydrogenase activity,the Shannon,Simpson,and Mcintosh indices of soil microbial communities were obviously increased under strawberry-rice rotation,soil solarization with rice bran,and calcium cyanamide（CaCN2）treatments,in addition,soil urease activity was significantly increased under strawberry-rice rotation and soil solarization with rice bran treatment,when compared with no fertilization.When compared with conventional fertilization treatment,strawberry-rice rotation and soil solarization with rice bran both significantly increased AWCD and Mcintosh index of soil microbial communities,meanwhile respectively increased soil urease and dehydrogenase activity.PCA analyses suggested that carbon utilization of soil microbial communities under strawberry-rice rotation,soil solarization with rice bran,and calcium cyanamide treatment was obviously different from that of conventional fertilization and no fertilization treatment.[Conclusion] strawberry-rice rotation and soil solarization with rice bran were effective agricultural managements to control soil biological degradation under Continuous Cropped Strawberry.
基金Supported by National Natural Science Foundation of China(31101534)Youth Foundation of Tutorial System,Fujian Academy of Agricultural Sciences(2013DQB-8)~~
文摘[Objective] The aim was to establish a set of soilless cultivation and management method of facility strawberry cultivation in the south of China.[Method] In the research,strawberry species,substrate formulae,cultivation modes and nutri ent solutions were selected and optimized.[Result] Three-dimensional strawberry cultivation was much suitable for Frandy,Hongbao and Gumei No.1; substrate with coconut shred-mushroom residue-peat soil ratio at 2∶2∶1 was the optimal; the treatment C3,containing Ca（NO3）2·4H2O,KNO3,（NH4）2HPO4,MgSO4·7H2O,and NH4NO3 at 280,303,62,246 and 80 mg/L,promoted strawberry growth,and the cultivated strawberry was large and thick in leaves,thick in stem,dark green in leaf color,with best fruit yield and quality.Among three cultivation modes,the density,space use ratio and yield in the treatment with PVC pipes in A-shape were all higher and the strawberry was easier and more convenient for picking.[Conclusion] The research provides references for soilless cultivation of strawberry in the south of China.
文摘[Objective] This study aimed to investigate the effect of different boric fer- tilizer on the growth, yield and quality of strawberry. [Method] With a strawberry cultivar Hongyan as the test material, water, borax and foliar disodium octoborate tetrahydrate was respectively sprayed to the leaves of strawberry four times from the squaring stage on. At the initial flowering stage and mature stage, the plant height, stem diameter, leaf area and petiole length of strawberry were measured; at the harvest time, the single fruit weight of strawberry was measured; and at the mature stage, the VC, sugar and organic acids contents in the strawberry fruit were determined. [Result] Compared with the control group, the spraying of foliar disodi- um octoborate tetrahydrate promoted the growth and significantly increased the plant height and leaf area of strawberry. However, no significant difference was found in root shoot ratio of strawberry between foliar disodium octoborate tetrahydrate treat- ment and the control group. Spraying of boric fertilizers significantly increased the single fruit weight and yield of strawberry. Compare with the control group, the spraying of foliar disodium octoborate tetrahydrate and borax increased the single fruit weight of strawberry by 20.14% and 4.86% respectively, and increased the yield of strawberry by 17.28% and 4.02% respectively. Compared with borax treat- ment and the control group, spraying of foliar disodium octoborate tetrahydrate im- proved the quality, i.e., increased the VC content, soluble solids content and sugar- acid ratio in strawberry fruit. The B content in leaf of strawberry was increased after foliar B fertilization. Among the three groups, the B content in leaf of strawberry ranked as disodium octoborate tetrahydrate treatment＇s 〉 borax treatment＇s 〉 con- trol＇s. [Conclusion] Spraying foliar disodium octoborate tetrahydrate can promote the growth, yield and quality of strawberry.
基金Supported by the National Natural Science Foundation of China(30370743)Foundation of Jiangxi Normal University (2441,2732)~~
文摘[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.
基金Supported by Fund for Independent Innovation in Agricultural Science and Technology of Jiangsu Province[CX(13)2012]~~
文摘This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extensively planted but not resistant to anthrax. The reciprocal crosses were performed using Benihoppe, as the female parent, four disease-resistant European cultivars Albion, Camino Real, Ven- tana and Virginia, and one Japanese cultivar Meiho as the male parent. Then, the seedling propagation coefficient, disease resistance, fruit quality and yield of F1 gen- eration of the combinations were detected and compared. Besides, the breeding and traits of a new cultivar F1-40 developed from the cross between Benihoppe and Mei- ho were introduced as an instance to prove the feasibility of the combinations/lines.
基金Supported by National Natural Science Foundation of China(31201402)Research Award Fund for Outstanding Young Scientist of Shandong Province(BS2011NY016)~~
文摘This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. According to the results of sin-gle factor test, an orthogonal experiment was designed to determine the optimum extraction conditions. The effects of the four factors on anthocyanins extraction from strawberry were listed here in an decreasing order： ethanol concentration 〉 solid-liq-uid ratio〉duration of ultrasonic treatment〉ultrasonic power. And the optimal condi-tions of ultrasonic extraction of anthocyanins from strawberry were： ultrasonic treat-ment of 30 min, solid-liquid ratio of 1：15, ethanol concentration of 60% and ultra-sonic power of 300 W. Under such conditions, the content of extracted antho-cyanins was 33.247 mg/100g, which was 1.3 folds higher than that by extraction without ultrasonic treatment.
基金Supported by Science and Technology Research Projects in Ningxia Hui Autonomous Region(KGZ-16-07-02)Opening project in National Engineering Laboratory of Tree Breeding in Beijing Forestry University~~
文摘[Objective] The aim was to investigate the application of plant growth regulator thidiazuron（TDZ）in strawberry anther culture.[Method]Different combinations of TDZ and naphthalene acetic acid（NAA）auxine with different concentrations were used for induction culture,and then its effect on callus induction and plant regeneration were observed.[Result] The combination of 1.0 mg/L TDZ＋0.5 mg/LNAA was effective to accelerating the differentiation and regeneration of strawberries,and the differentiation ratio was as high as 75%.However,TDZ showed insignificant effect on strawberries anther induction.[Conclusion]This research had provided reference for large-scale production of strawberry through anther culture.
基金Supported by Zhenjiang Science&Technology Pillar Program(NY2013001)~~
文摘It is proved that the treatment with white mulch and black thin films per- formed the best in terms of heat preservation in cultivation of strawberry with shelves. In winter, drip irrigation performed significantly in transporting hot water through solar energy. The combination of the two methods resolved the issue of heat preservation difficulty and guaranteed growth of strawberry in winter.
基金Supported by Agricultural Science and Technology Support Program of Jiangsu Province(BE2012378)2011"Six Talent Peaks"Program of Jiangsu Province(2013-NY-001)Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province[CX(11)2018]~~
文摘[Objective] This study aimed to identify strawberry anthracnose pathogens and screen appropriate germicides. [Method] Strawberry anthracnose pathogens in Jurong City of Jiangsu Province were isolated and identified. The toxicities of sever-al germicides to Col etotrichum fragariae were determined with indoor mycelial growth inhibition assay. Field control test was preformed to further verify the control efficacy of germicides. [Result] The isolated strawberry anthracnose pathogenic strains were identified as C. gloeosporioide from genus Col etotrichum of subphylum Deuteromycotina. Prochloraz, pyrazole Kresoxim and hexaconazole al exhibited good indoor control effects; 25% pyrazole Kresoxim EC2000-fold solution, 24% azoxys-trobin＆#183;hexaconazole （azoxystrobin∶hexaconazole=1∶2） SC1500-fold solution and 25%prochloraz EC1000-fold solution al exhibited significant field control efficacy. [Con-clusion] The study provides reference for the alternate use of germicides in actual production.
文摘From middle December, 2012 to early January, 2013, Jinan City was in- fluenced by cold airs and successive rainy and snowy days lasted for 13 d. Consequently, strawberry in greenhouse was seriously affected. In the research, the causes of strawberry cold injury were surveyed and analyzed, to provide references for scientific management for farmers.
基金Supported by Demonstration and Promotion Project of Shanghai Municipal Committee of Agriculture[HNKT(2015)2-7]Jiangsu Agricultural"Three New Engineering"Project[SXGC(2017)208]Jiangsu Agricultural Science and Technology Innovation Fund[CX(15)1029]~~
文摘In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.
