Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alp...Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alpha-olide 2.展开更多
A novel eremophilane dimer,named fischelactone（1）,was isolated from the roots of Ligularia fischeri.Complete structural elucidation of this compound was performed by HRESIMS,IR,1D NMR and 2D NMR（;H-;H COSY,HMBC,HMQ...A novel eremophilane dimer,named fischelactone（1）,was isolated from the roots of Ligularia fischeri.Complete structural elucidation of this compound was performed by HRESIMS,IR,1D NMR and 2D NMR（;H-;H COSY,HMBC,HMQC and NOESY） data.展开更多
Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still li...Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.展开更多
A hydroponic cultivation system was established to improve the nutraceutical properties of Ligularia fischeri(Ledeb.) Turcz, during which nutrient uptake by the plant from nutrient solution was measured using inductiv...A hydroponic cultivation system was established to improve the nutraceutical properties of Ligularia fischeri(Ledeb.) Turcz, during which nutrient uptake by the plant from nutrient solution was measured using inductive coupled plasma-atomic emission spectroscopy(ICP-AES).Based on the obtained data, the uptake of macro and micro elements per gram of fresh weight was calculated. The uptake of macro-elements of NH_4^+-N,NO_3^--N, K, S, P, Ca,and Mg were 1,62,4.27, 8.41,1.19, 2.59, 2,79, and 0.84 mg·g^(-1) FW and micro-elements of B, Fe, Mn, Mo, Cu, and Zn were 9.91,22.31,25.73,2.51,2.91, and 5.07 μg·g^(-1) FW. Moreover, the effects of cultivation systems on growth and phytochemical composition of L.fischeri were compared. The greatest biomass was observed in the hydroponic cultivation system with continuous circulation nutrient solution compared to natural soil and Tosilee media based culture systems. The recirculated hydroponic system significantly increased the total phenol contents of the leaf, petiole, and root extracts by 17.6%, 30.60%, and 20.9% more compared to the soil grown. The recirculated hydroponic system treatment significantly increased the total antioxidant capacity of root extracts by 55.9% more compared to the soil treatment. Based on the contents of elements, total phenolic and flavonoid, it was concluded that hydroponic cultivation system is the optimal method to enhance medicinal value.展开更多
文摘为获得酶学性质优良的α-半乳糖苷酶,研究根据Gen Bank数据库提供的嗜热微生物Neosartorya fischeri的α-半乳糖苷酶的氨基酸序列,经密码子优化合成该酶的编码基因。将合成的基因转化大肠杆菌BL21进行原核表达,获得重组α-半乳糖苷酶,并对酶学性质进行研究。结果显示:以棉籽糖为底物时,酶的最适反应温度为55℃,最适p H 6.0,有较好的热稳定性和酸碱稳定性。Fe2+(铁)、Mn2+(锰)和Ca2+(钙)离子可使酶活性大幅提高,而Cu2+(铜)离子则可强烈抑制酶的活性,Pb2+(铅)、Ba2+(钡)、Al3+(铝)、EDTA(乙二胺四乙酸)和SDS(十二烷基硫酸钠)对酶活也有一定的抑制作用。在模拟胃肠环境时发现该酶有良好的稳定性,肠液对该酶甚至有轻微的激活作用。
文摘Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alpha-olide 2.
基金financially supported by State Key Laboratory of Applied Organic Chemistry,Lanzhou University, China and Natural Science Foundation of Shandong Province,China(No.Y2008D23).
文摘A novel eremophilane dimer,named fischelactone（1）,was isolated from the roots of Ligularia fischeri.Complete structural elucidation of this compound was performed by HRESIMS,IR,1D NMR and 2D NMR（;H-;H COSY,HMBC,HMQC and NOESY） data.
文摘聚半乳糖醛酸酶在食品和饲料行业具有很大的应用价值,发现新的聚半乳糖醛酸酶不仅能够补充该酶的相关信息,还能为工业应用提供更多选择。从一株嗜热费希新萨托菌（Neosartorya fischeri P1）的基因组中克隆得到一个外切聚半乳糖醛酸酶基因（Nf-pg）,将Nf-pg在毕赤酵母GS115中进行异源表达,重组酶Nf-PG纯化后进行性质测定。Nf-PG在p H 4.5和55℃时活性最高,在p H 2.0~9.0具有较好的稳定性,50℃热处理60 min仍能保持80%的剩余酶活;去除N-糖基化的酶蛋白Nf-PG-D最适温度为50℃,热稳定性降低,最适p H和酸碱稳定性无明显改变。产物分析证明Nf-PG是严格的外切聚半乳糖醛酸酶,优异的性质使其成为食品加工行业的良好选择。
文摘Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.
基金supported from the grants of the BK21 Plus program (Brain Korea 21) of the Ministry of Education, Republic of Korea
文摘A hydroponic cultivation system was established to improve the nutraceutical properties of Ligularia fischeri(Ledeb.) Turcz, during which nutrient uptake by the plant from nutrient solution was measured using inductive coupled plasma-atomic emission spectroscopy(ICP-AES).Based on the obtained data, the uptake of macro and micro elements per gram of fresh weight was calculated. The uptake of macro-elements of NH_4^+-N,NO_3^--N, K, S, P, Ca,and Mg were 1,62,4.27, 8.41,1.19, 2.59, 2,79, and 0.84 mg·g^(-1) FW and micro-elements of B, Fe, Mn, Mo, Cu, and Zn were 9.91,22.31,25.73,2.51,2.91, and 5.07 μg·g^(-1) FW. Moreover, the effects of cultivation systems on growth and phytochemical composition of L.fischeri were compared. The greatest biomass was observed in the hydroponic cultivation system with continuous circulation nutrient solution compared to natural soil and Tosilee media based culture systems. The recirculated hydroponic system significantly increased the total phenol contents of the leaf, petiole, and root extracts by 17.6%, 30.60%, and 20.9% more compared to the soil grown. The recirculated hydroponic system treatment significantly increased the total antioxidant capacity of root extracts by 55.9% more compared to the soil treatment. Based on the contents of elements, total phenolic and flavonoid, it was concluded that hydroponic cultivation system is the optimal method to enhance medicinal value.
