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调胃承气汤对急性脑梗死合并胃损伤大鼠SCF/c-Kit信号通路的影响
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作者 张增 刘欢欢 +5 位作者 兰雅文 王春燕 高召凯 安朋朋 袁山政 唐明 《中国民间疗法》 2026年第4期72-76,共5页
目的:本研究评估调胃承气汤对急性脑梗死合并胃损伤大鼠干细胞因子/酪氨酸激酶受体(SCF/c-Kit)信号通路的影响。方法:将80只处于10周龄且健康的雄性Wistar大鼠随机分为5组,Ⅰ组为对照组、Ⅱ组为假手术组、Ⅲ组为模型组、Ⅳ组为中药组、... 目的:本研究评估调胃承气汤对急性脑梗死合并胃损伤大鼠干细胞因子/酪氨酸激酶受体(SCF/c-Kit)信号通路的影响。方法:将80只处于10周龄且健康的雄性Wistar大鼠随机分为5组,Ⅰ组为对照组、Ⅱ组为假手术组、Ⅲ组为模型组、Ⅳ组为中药组、Ⅴ组为西药组。Ⅳ、Ⅴ组分别给予调胃承气汤(113.40mg/d)、雷贝拉唑钠肠溶片悬浊液(0.18mg/d)灌胃,其余各组给予等剂量氯化钠溶液,连续给药7d。分别在3、7d时,观察各组大鼠胃组织苏木精-伊红(HE)染色情况,且进行胃黏膜损伤病理评分;7d后,通过蛋白质印迹法(Westernblot)、反转录聚合酶链式反应(RT-PCR)分别检测大鼠胃组织中的c-Kit、SCF蛋白及mRNA的表达情况。结果:灌胃3、7d时,Ⅲ、Ⅳ、Ⅴ组大鼠的胃黏膜损伤病理评分均高于Ⅰ、Ⅱ组(P<0.05);Ⅰ、Ⅱ组大鼠胃黏膜损伤病理评分比较,差异无统计学意义(P>0.05);Ⅳ、Ⅴ组大鼠胃黏膜损伤病理评分均低于Ⅲ组(P<0.05),且Ⅳ组的评分低于Ⅴ组(P<0.05)。灌胃7d时,Ⅳ、Ⅴ组的胃黏膜损伤病理评分均低于灌胃3d时(P<0.05);Ⅲ、Ⅳ、Ⅴ组大鼠胃组织中SCF、c-Kit的mRNA和蛋白表达水平均低于Ⅰ、Ⅱ组(P<0.05);Ⅳ、Ⅴ组的mRNA和蛋白表达水平均高于Ⅲ组(P<0.05),且Ⅳ组均高于Ⅴ组(P<0.05)。结论:调胃承气汤可以在一定程度上改善脑梗死合并胃损伤大鼠的胃黏膜状态,这可能与调胃承气汤调节大鼠胃组织中SCF、c-Kit的mRNA及蛋白表达有关。 展开更多
关键词 急性脑梗死 胃损伤 调胃承气汤 scf/c-Kit信号通路
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A method for enhancing the performance of infrared filters based on ratemodulated deposition of germanium films
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作者 LIU Bao-Jian LI Da-Qi +7 位作者 DUAN Wei-Bo YU De-Ming CAI Qing-Yuan YU Tian-Yan JIANG Lin YANG Yu-Ting ZHUANG Qiu-Hui ZHENG Yu-Xiang 《红外与毫米波学报》 北大核心 2026年第1期157-165,共9页
This study systematically investigated the influence of deposition rate on the structure,broadband opti⁃cal properties(1.0-13.0μm),and stress characteristics of Germanium(Ge)films.Additionally,a method for enhancing ... This study systematically investigated the influence of deposition rate on the structure,broadband opti⁃cal properties(1.0-13.0μm),and stress characteristics of Germanium(Ge)films.Additionally,a method for enhancing the performance of infrared filters based on rate-modulated deposition of Ge films was proposed.The optical absorption of Ge films in the short-wave infrared(SWIR)and long-wave infrared(LWIR)bands can be effectively reduced by modulating the deposition rate.As the deposition rate increases,the Ge films maintain an amorphous structure.The optical constants of the films in the 1.0-2.5μm and 2.5-13.0μm bands were precisely determined using the Cody-Lorentz model and the classical Lorentz oscillator model,respectively.Notably,high⁃er deposition rates result in a gradual increase in the refractive index.The extinction coefficient increases with the deposition rate in the SWIR region,attributed to the widening of the Urbach tail,while it decreases in the LWIR region due to the reduced absorption caused by the Ge-O stretching mode.Additionally,the films exhibit a tensile stress that decreases with increasing deposition rate.Finally,the effectiveness of the proposed fabrication method for an infrared filter with Ge films deposited at an optimized rate was demonstrated through practical examples.This work provides theoretical and technical support for the application of Ge films in high-performance infrared filters. 展开更多
关键词 optical coatings germanium film optical absorption infrared filter
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基于SCF/c-Kit通路探讨加味安肠汤联合阿洛司琼对腹泻型肠易激综合征大鼠的作用机制
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作者 袁小晶 王凡 《世界中西医结合杂志》 2026年第1期53-59,共7页
目的基于干细胞因子(Stem cell factor,SCF)/细胞源性酪氨酸激酶受体通路(cellular tyrosine kinase receptor pathway,c-Kit)探讨加味安肠汤联合阿洛司琼对腹泻型肠易激综合征大鼠的作用机制。方法将100只同月龄SPF级Wistar大鼠,随机选... 目的基于干细胞因子(Stem cell factor,SCF)/细胞源性酪氨酸激酶受体通路(cellular tyrosine kinase receptor pathway,c-Kit)探讨加味安肠汤联合阿洛司琼对腹泻型肠易激综合征大鼠的作用机制。方法将100只同月龄SPF级Wistar大鼠,随机选取80只大鼠进行造模,制备腹泻型肠易激综合征大鼠模型,造模成功后采用随机数字表法将大鼠分为模型组(给予10 ml/kg生理盐水灌胃)、阿洛司琼组(给予1.008 mg/kg阿洛司琼灌胃)、加味安肠汤组(给予18.9 g/kg加味安肠汤灌胃)、中西医结合组(给予1.008 mg/kg阿洛司琼联合18.9 g/kg加味安肠汤灌胃),每组20只,剩余20只大鼠设为空白组。末次治疗后8 h,将各组大鼠麻醉(10%水合氯醛)后处死,取腹主动脉血10 ml及收集结肠组织,观察各组大鼠一般情况、5-羟色胺(5-hydroxytryptamine,5-HT)、SCF水平;免疫荧光法测定各组大鼠c-Kit阳性卡哈尔间质细胞(Interstitial cells of cajal,ICC)水平及Western blot测定各组大鼠SCF、c-Kit蛋白水平。结果与空白组比较,模型组体质量、SCF水平、ICC荧光强度、c-Kit蛋白水平明显降低,5-HT水平明显升高,差异有统计学意义(P<0.05),表示造模成功,且造模后大鼠SCF水平、ICC荧光强度、c-Kit蛋白水平呈异常升高,5-HT水平呈异常下降;治疗1、2周后,与模型组比较,阿洛司琼组、加味安肠汤组、中西医结合组体质量明显升高,差异有统计学意义(P<0.05);与阿洛司琼组和加味安肠汤组比较,中西医结合组体质量明显升高,差异有统计学意义(P<0.05)。与模型组比较,阿洛司琼组、加味安肠汤组、中西医结合组5-HT水平下降,SCF水平、ICC荧光强度、SCF、c-Kit蛋白水平升高,差异有统计学意义(P<0.05);与阿洛司琼组和加味安肠汤组比较,中西医结合组5-HT水平明显下降,SCF水平、ICC荧光强度、c-Kit蛋白水平升高,差异有统计学意义(P<0.05)。结论加味安肠汤联合阿洛司琼可通过SCF/c-Kit通路发挥治疗腹泻型肠易激综合征的作用。 展开更多
关键词 scf/c-Kit通路 加味安肠汤 阿洛司琼 腹泻型肠易激综合征
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Cavity ring-down spectroscopy CO gas sensor integrating principal component analysis with savitzky-golay filtering
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作者 GUO Zi-long SHI Cheng-rui +4 位作者 DONG Yuan-yuan ZHANG Lei SUN Xiao-yuan SUN Jing-jing ZHOU Sheng 《中国光学(中英文)》 北大核心 2026年第1期179-189,共11页
The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recogni... The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility. 展开更多
关键词 cavity ring-down spectroscopy CO gas sensor principal component analysis Savitzky-Golay filter
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Performance analysis of state of charge and state of health prediction using Kalman filter techniques with battery parameter variation
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作者 Ranagani Madhavi Indragandhi Vairavasundaram 《Global Energy Interconnection》 2026年第1期143-158,共16页
Accurate estimation of the State of Charge(SOC),State of Health(SOH),and Terminal Resistance(TR)is crucial for the effective operation of Battery Management Systems(BMS)in lithium-ion batteries.This study conducts a c... Accurate estimation of the State of Charge(SOC),State of Health(SOH),and Terminal Resistance(TR)is crucial for the effective operation of Battery Management Systems(BMS)in lithium-ion batteries.This study conducts a comprehensive comparative analysis of four Kalman filter variants Extended Kalman Filter(EKF),Extended Kalman-Bucy Filter(EKBF),Unscented Kalman Filter(UKF),and Unscented Kalman-Bucy Filter(UKBF)under varying battery parameter conditions.These include temperature fluctuation,self-discharge,current direction,cell capacity,process noise,and measurement noise.Our findings reveal significant variations in the performance of SOC and SOH predictions across filters,emphasizing that UKF demonstrates superior robustness to noise,while EKF performs better under accurate system dynamics.The study underscores the need for adaptive filtering strategies that can dynamically adjust to evolving battery parameters,thereby enhancing BMS reliability and extending battery lifespan. 展开更多
关键词 State of chargeState of health Extended Kalman filter Extended Kalman Bucy filter Unscented Kalman filter Unscented Kalman Bucy filter
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Miniaturized bandpass filter with a wide upper stopband using isomeric resonators in a cavity
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作者 Chengyang ZHANG Ying XUE +1 位作者 Qingyuan LU Jianxin CHEN 《ENGINEERING Information Technology & Electronic Engineering》 2026年第1期81-86,共6页
1 Introduction Recently,the increasing demand for advanced telecommunication systems has spurred extensive research into bandpass filters(BPFs),with particular emphasis on miniaturization,reduction of insertion loss(I... 1 Introduction Recently,the increasing demand for advanced telecommunication systems has spurred extensive research into bandpass filters(BPFs),with particular emphasis on miniaturization,reduction of insertion loss(IL),and enhancement of upper stopband rejection(Huang et al.,2021;Snyder et al.,2021;Lin et al.,2023;Zeng et al.,2023). 展开更多
关键词 bandpass filters bpfs advanced telecommunication systems upper stopband rejection isomeric resonators cavity filters enhancement upper stopband rejection huang miniaturized bandpass filters insertion loss
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An Improved High-Degree Cubature Particle Filter and its Application in Bearing-only Tracking
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作者 Yanqi Niu Dandan Zhu Yaan Li 《哈尔滨工程大学学报(英文版)》 2026年第1期300-311,共12页
In this study,a fifth-degree cubature particle filter(5CPF)is proposed to address the limited estimation accuracy in traditional particle filter algorithms for bearings-only tracking(BOT).This algorithm calculates the... In this study,a fifth-degree cubature particle filter(5CPF)is proposed to address the limited estimation accuracy in traditional particle filter algorithms for bearings-only tracking(BOT).This algorithm calculates the recommended density function by introducing a fifth-degree cubature Kalman filter algorithm to guide particle sampling,which effectively alleviates the problem of particle degradation and significantly improves the estimation accuracy of the filter.However,the 5CPF algorithm exhibits high computational complexity,particularly in scenarios with a large number of particles.Therefore,we propose the extended Kalman filter(EKF)-5CPF algorithm,which employs an EKF to replace the time update step for each particle in the 5CPF.This enhances the algorithm’s real-time capability while maintaining the high precision advantage of the 5CPF algorithm.In addition,we construct bearing-only dual-station and single-motion station target tracking systems,and the filtering performances of 5CPF and EKF-5CPF algorithms under different conditions are analyzed.The results show that both the 5CPF algorithm and EKF-5CPF have strong robustness and can adapt to different noise environments.Furthermore,both algorithms significantly outperform traditional nonlinear filtering algorithms in terms of convergence speed,tracking accuracy,and overall stability. 展开更多
关键词 Nonlinear filtering Fifth-degree cubature particle filter EKF-5CPF Bearings-only target motion analysis
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Trans-reflective color filters with brilliant colors by integrating organic dyes with photonic crystals
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作者 Shi Li Yong Qi +4 位作者 Wenbin Niu Suli Wu Bingtao Tang Wei Ma Shufen Zhang 《Smart Molecules》 2026年第1期106-117,共12页
Color filters are essential components for optical modulation.However,conventional filters are restricted to operating exclusively in either reflective or transmissive mode.Furthermore,they suffer from limited UV and ... Color filters are essential components for optical modulation.However,conventional filters are restricted to operating exclusively in either reflective or transmissive mode.Furthermore,they suffer from limited UV and thermal stability,low color purity,and exhibit identical coloration on both surfaces.Herein,we propose a novel design strategy for trans-reflective color filters by integrating the absorptive properties of dye-doped polysulfone(PSU)with the diffractive capabilities of photonic crystals.This composite filter achieved broad-spectrum transmission with deep color outputs—yellow(0.410,0.510),magenta(0.446,0.231),and cyan(0.201,0.425)—closely aligned with standard color space coordinates.By tuning the refractive index of CeO_(2)@SiO_(2)nanoparticles to match dye-based PSU matrix,the transmittance of filters exceeded 70%.Moreover,dye-mediated absorption reduces the scattering light,thereby enhancing reflection color purity(full width at half maxima(FWHM)=25 nm)and producing vibrant blue,green,and red hues.The incorporation of UV-absorbing CeO_(2)@SiO_(2)nanoparticles effectively mitigated dye photodegradation,yielding exceptional UV stability(ΔT<2%under prolonged UV exposure).The filters also exhibited outstanding thermal stability(ΔT<1%after 30 min heat treatment at 230°C).This work establishes a robust materials design framework for multifunctional optical filters,advancing the development of highfidelity dual-mode color systems for next-generation display technologies. 展开更多
关键词 DYES photonic crystals stability trans-reflective color filters
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HCF-MFGB:Hybrid Collaborative Filtering Based on Matrix Factorization and Gradient Boosting
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作者 Salahudin Robo Triyanna Widiyaningtyas Wahyu Sakti Gunawan Irianto 《Computers, Materials & Continua》 2026年第2期1630-1648,共19页
Recommendation systems are an integral and indispensable part of every digital platform,as they can suggest content or items to users based on their respective needs.Collaborative filtering is a technique often used i... Recommendation systems are an integral and indispensable part of every digital platform,as they can suggest content or items to users based on their respective needs.Collaborative filtering is a technique often used in various studies,which produces recommendations by analyzing similarities between users and items based on their behavior.Although often used,traditional collaborative filtering techniques still face the main challenge of sparsity.Sparsity problems occur when the data in the system is sparse,meaning that only a portion of users provide feedback on some items,resulting in inaccurate recommendations generated by the system.To overcome this problem,we developed aHybrid Collaborative Filtering model based onMatrix Factorization andGradient Boosting(HCF-MFGB),a new hybrid approach.Our proposed model integrates SVD++,the XGBoost ensemble learning algorithm,and utilizes user demographic data and meta items.We utilize information,both explicitly and implicitly,to learn user preference patterns using SVD++.The XGBoost algorithm is used to create hundreds of decision trees incrementally,thereby improving model accuracy.Meanwhile,user demographic and meta-item data are clustered using the K-Means Clustering algorithm to capture similarities in user and item characteristics.This combination is designed to improve rating prediction accuracy by reducing reliance on minimal explicit rating data,while addressing sparsity issues in movie recommendation systems.The results of experiments on the MovieLens 100K,MovieLens 1M,and CiaoDVD datasets show significant improvements,outperforming various other baselinemodels in terms of RMSE and MAE.On theMovieLens 100K dataset,the HCF-MFGB model obtained an RMSE value of 0.853 and an MAE value of 0.674.On theMovieLens 1M dataset,the HCF-MFGB model obtained an RMSE value of 0.763 and an MAE value of 0.61.On the CiaoDCD dataset,the HCF-MFGB model achieved an RMSE value of 0.718 and an MAE value of 0.495.These results confirm a significant improvement in movie recommendation accuracy with the proposed approach. 展开更多
关键词 Recommendation systems hybrid collaborative filtering SVD++ XGBoost K-Means clustering user demographics meta item
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Adaptive Intelligent Control of a Lumped EvaporatorModel Using Wavelet-Based Neural PID with IIR Filtering
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作者 M.A.Vega Navarrete P.J.Argumedo Teuffer +2 位作者 C.M.RodríguezRomán L.E.Marrón Ramírez E.A.IslasNarvaez 《Frontiers in Heat and Mass Transfer》 2026年第1期354-374,共21页
This article presents an adaptive intelligent control strategy applied to a lumped-parameter evaporator model,i.e.,a simplified dynamic representation treating the evaporator as a single thermal node with uniform temp... This article presents an adaptive intelligent control strategy applied to a lumped-parameter evaporator model,i.e.,a simplified dynamic representation treating the evaporator as a single thermal node with uniform temperature distribution,suitable for control design due to its balance between physical fidelity and computational simplicity.The controller uses a wavelet-based neural proportional,integral,derivative(PID)controller with IIR filtering(infinite impulse response).The dynamic model captures the essential heat and mass transfer phenomena through a nonlinear energy balance,where the cooling capacity“Qevap”is expressed as a non-linear function of the compressor frequency and the temperature difference,specifically,Q_(evap)=k_(1)u(T_(in)−T_(e))with u as compressor frequency,Te evaporator temperature,and Tin inlet fluid temperature.The operating conditions of the system,in general terms,focus on the following variables,the overall thermal capacity is 1000 J/K,typical for small-capacity heat exchangers,The mass flow is 0.05 kg/s,typical for secondary liquid cooling circuits,the overall loss coefficient of 50 W/K that corresponds to small evaporators with partial insulation,the temperatures(inlet)of 10℃and the temperature of environment of 25℃,thermal load of 200 W that corresponds to a small-scaled air conditioning applications.To handle system nonlinearities and improve control performance,aMorlet wavelet-based neural network(Wavenet)is used to dynamically adjust the PID gains online.An IIR filter is incorporated to smooth the adaptive gains,improving stability and reducing oscillations.In contrast to prior wavelet-or neural-adaptive PID controllers in HVAC applications,which typically adjust gains without explicit filtering or not tailored to evaporator dynamics,this work introduces the first PID–Wavenet scheme augmented with an IIR-based stabilization layer,specifically designed to address the combined challenges of nonlinear evaporator behavior,gain oscillation,and real-time implementability.The proposed controller(PID-Wavenet+IIR)is implemented and validated inMATLAB/Simulink,demonstrating superior performance compared to a conventional PID tuned using Simulink’s auto-tuning function.Key results include a reduction in settling time from 13.3 to 8.2 s,a reduction in overshoot from 3.5%to 0.8%,a reduction in steady-state error from 0.12℃ to 0.02℃and a 13%reduction in energy overall consumption.The controller also exhibits greater robustness and adaptability under varying thermal loads.This explicit integration of wavelet-driven adaptation with IIR-filtered gain shaping constitutes the main methodological contribution and novelty of the work.These findings validate the effectiveness of the wavelet-based adaptive approach for advanced thermal management in refrigeration and HVAC systems,with potential applications in controlling variable-speed compressors,liquid chillers,and compact cooling units. 展开更多
关键词 Evaporator modeling heat transfer systems adaptive control PID-Wavenet IIR filtering dynamic cooling optimization
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Synthesis and Design of Generalized Strongly Coupled Resonator Quartet Combline Filters with Redundant Resonance
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作者 Xiong Zhi’ang Fan Jiyuan +3 位作者 Zhao Ping Zhou Jinzhu Shen Nan Wu Qingqiang 《ZTE Communications》 2026年第1期88-96,共9页
This article proposes a generalized strongly coupled resonator quartet(GSCRQ)filter along with its synthesis approach.By introducing out-of-band reflection zeros(RZs),the proposed GSCRQ can generate a transmission zer... This article proposes a generalized strongly coupled resonator quartet(GSCRQ)filter along with its synthesis approach.By introducing out-of-band reflection zeros(RZs),the proposed GSCRQ can generate a transmission zero on each side of the passband without negative couplings.The coupling coefficients in this coupling structure change with the positions of the out-of-band RZs.Thus,the GSCRQ configuration admits flexible design solutions.For GSCRQ coaxial combline filters,all couplings can be implemented as inductive couplings,simplifying the design and manufacturing process.In this article,a 6-2 filter in the GSCRQ configuration is synthesized and designed.The simulated results of the designed filter agree very well with the theoretical characteristics. 展开更多
关键词 filter synthesis generalized strongly coupled resonator quartets(GSCRQ) out-of-band reflection zero transmission zero
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Representation Then Augmentation:Wide Graph Clustering Network With Multi-Order Filter Fusion and Double-Level Contrastive Learning
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作者 Youqing Wang Tianxiang Zhao +3 位作者 Mingliang Cui Junbin Gao Li Liang Jipeng Guo 《IEEE/CAA Journal of Automatica Sinica》 2026年第2期421-435,共15页
Deep graph contrastive clustering has attracted widespread attentions due to its self-supervised representation learning paradigm and superior clustering performance.Although,two challenges emerge and result in high c... Deep graph contrastive clustering has attracted widespread attentions due to its self-supervised representation learning paradigm and superior clustering performance.Although,two challenges emerge and result in high computational costs.Most existing contrastive methods adopt the data augmentation and then representation learning strategy,where representation learning with trainable graph convolution is coupled with complex and fixed data augmentation,inevitably limiting the efficiency and flexibility.The similarity metric between positive-negative sample pairs is complex and contrastive objective is partial,limiting the discriminability of representation learning.To solve these challenges,a novel wide graph clustering network(WGCN)adhering to representation and then augmentation framework is proposed,which mainly consists of multiorder filter fusion(MFF)and double-level contrastive learning(DCL)modules.Specifically,the MFF module integrates multiorder low-pass filters to extract smooth and multi-scale topological features,utilizing self-attention fusion to reduce redundancy and obtain comprehensive embedding representation.Further,the DCL module constructs two augmented views by the parallel parameter-unshared Siamese encoders rather than complex augmentations on graph.To achieve simple yet effective self-supervised learning,representation self-supervision and structural consistency oriented double-level contrastive loss is designed,where representation self-supervision maximizes the agreement between pairwise augmented embedding representations and structural consistency promotes the mutual information correlation between appending neighborhoods with similar semantics.Extensive experiments on six benchmark datasets demonstrate the superiority of the proposed WGCN,especially highlighting its time-saving characteristic.The code could be available in the https://github.com/Tianxiang Zhao0474/WGCN. 展开更多
关键词 Deep graph clustering(DGC) double-level contrastive learning(DCL) multi-order low-pass filter self-supervised representation learning structural consistency
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缩泉润肠方通过促进SCF、c-Kit表达改善小鼠老年性便秘 被引量:2
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作者 孙光军 张晓雨 +2 位作者 鲍新坤 肖文洁 林爱珍 《广州中医药大学学报》 2025年第4期956-961,共6页
【目的】观察缩泉润肠方对老年性便秘小鼠的治疗作用及机制。【方法】将老年昆明小鼠随机分为便秘组、缩泉润肠方组、ISCK03[干细胞因子(SCF)、酪氨酸激酶生长因子受体(c-Kit)信号通路抑制剂]组、缩泉润肠方+ISCK03组、正常组,每组12只... 【目的】观察缩泉润肠方对老年性便秘小鼠的治疗作用及机制。【方法】将老年昆明小鼠随机分为便秘组、缩泉润肠方组、ISCK03[干细胞因子(SCF)、酪氨酸激酶生长因子受体(c-Kit)信号通路抑制剂]组、缩泉润肠方+ISCK03组、正常组,每组12只。除正常组外,其他组老年昆明小鼠均通过灌胃150 mg/kg复方地芬诺酯混悬液构建便秘模型。建模成功后,各组进行相应干预2周。干预结束后,测定小鼠粪便粒数、粪便含水量,酶联免疫吸附分析(ELISA)检测血清中白细胞介素(IL)-1β、IL-6、胃动素、胃泌素、5-羟色胺(5-HT)、血管活性肠肽(VIP)水平,测定小鼠胃排空率和小肠推进率,苏木精-伊红(HE)染色法观察结肠组织的病理变化,Western Blot法检测结肠组织中SCF、c-Kit蛋白表达。【结果】与正常组比较,便秘组小鼠结肠组织的细胞结构被破坏,肌肉层变薄,黏膜上皮出现损伤,粪便粒数,粪便含水量,血清中胃动素、胃泌素、5-HT水平,胃排空率、小肠推进率,结肠组织中SCF、c-Kit蛋白表达水平降低,血清中IL-1β、IL-6、VIP水平升高,差异均有统计学意义(P<0.05);与便秘组比较,缩泉润肠方组小鼠结肠组织损伤有所改善,粪便粒数、粪便含水量,血清中胃动素、胃泌素、5-HT水平,胃排空率、小肠推进率,结肠组织中SCF、c-Kit蛋白表达水平升高,血清中IL-1β、IL-6、VIP水平降低,差异均有统计学意义(P<0.05),而ISCK03组小鼠对应指标变化趋势与缩泉润肠方组相反(P<0.05);与缩泉润肠方组比较,缩泉润肠方+ISCK03组小鼠结肠组织损伤加剧,粪便粒数,粪便含水量,血清中胃动素、胃泌素、5-HT水平,胃排空率、小肠推进率,结肠组织中SCF、c-Kit蛋白表达水平降低,血清中IL-1β、IL-6、VIP水平升高,差异均有统计学意义(P<0.05)。【结论】缩泉润肠方可有效改善小鼠老年性便秘,其治疗作用与上调SCF、c-Kit表达,进而促进肠蠕动有关。 展开更多
关键词 缩泉润肠方 老年性便秘 scf/c-Kit信号通路 胃排空 肠蠕动 脑肠肽 小鼠
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枳实对慢传输型便秘小鼠肠道动力及SCF/c-Kit通路的影响
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作者 杜燕红 林爱珍 +1 位作者 刘枭汉 尹诗文 《广州中医药大学学报》 2025年第8期2028-2034,共7页
【目的】观察枳实对慢传输型便秘(STC)小鼠的治疗作用及机制。【方法】采用盐酸洛哌丁灌胃法构建STC小鼠模型。将造模成功的小鼠随机分为模型组,枳实低、高剂量组和枳实高剂量+Masitinib[酪氨酸激酶受体(c-Kit)抑制剂]组,另设正常组对... 【目的】观察枳实对慢传输型便秘(STC)小鼠的治疗作用及机制。【方法】采用盐酸洛哌丁灌胃法构建STC小鼠模型。将造模成功的小鼠随机分为模型组,枳实低、高剂量组和枳实高剂量+Masitinib[酪氨酸激酶受体(c-Kit)抑制剂]组,另设正常组对照。分组干预后,测定小鼠肠道推进率和6 h排便粒数、粪便含水率,苏木精-伊红(HE)染色法观察结肠组织形态变化,酶联免疫吸附分析(ELISA)检测结肠组织5-羟色胺(5-HT)、血管活性肠多肽(VIP)、P物质(SP)、一氧化氮(NO)含量及一氧化氮合酶(NOS)活性,实时荧光定量逆转录聚合酶链反应(qRT-PCR)法检测结肠组织干细胞因子(SCF)和c-Kit基因表达,Western Blot法检测结肠组织水通道蛋白3(AQP3)、水通道蛋白8(AQP8)、SCF和c-Kit蛋白表达。【结果】与正常组比较,模型组小鼠结肠组织明显损伤,排便粒数和粪便含水率,肠道推进率,结肠组织SP含量、c-Kit和SCF基因和蛋白表达水平降低,结肠组织5-HT、VIP、NO含量和NOS活性,结肠组织AQP3和AQP8蛋白表达水平升高,差异均有统计学意义(P<0.05);与模型组比较,枳实低、高剂量组小鼠结肠组织损伤明显改善,排便粒数和粪便含水率,肠道推进率,结肠组织SP含量、c-Kit和SCF基因和蛋白表达水平升高,结肠组织5-HT、VIP、NO含量和NOS活性,结肠组织AQP3和AQP8蛋白表达水平降低,差异均有统计学意义(P<0.05);Masitinib可部分逆转枳实对STC小鼠排便的改善作用(P<0.05)。【结论】枳实可提高STC小鼠肠道动力,减轻结肠组织损伤,改善排便情况,其机制可能与激活SCF/c-Kit通路有关。 展开更多
关键词 枳实 慢传输型便秘 肠道动力 scf/c-Kit通路 Masitinib 小鼠
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基于SCF/c-Kit通路研究首荟通便胶囊治疗慢传输型便秘的作用及机制
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作者 付智慧 边猛 王娟 《贵州医药》 2025年第8期1184-1188,共5页
目的基于SCF/c-Kit通路系统研究首荟通便胶囊治疗慢传输型便秘(STC)的作用及机制。方法构建STC大鼠模型,构建成功后,阳性药组、首荟通便胶囊组灌胃给予相应药物,正常组、模型组灌胃给予生理盐水,每组均6只大鼠。观察并记录各组大鼠大便... 目的基于SCF/c-Kit通路系统研究首荟通便胶囊治疗慢传输型便秘(STC)的作用及机制。方法构建STC大鼠模型,构建成功后,阳性药组、首荟通便胶囊组灌胃给予相应药物,正常组、模型组灌胃给予生理盐水,每组均6只大鼠。观察并记录各组大鼠大便情况、精神状态等一般特征,测量并计算末次给药30 min后的肠道推进率,苏木素—伊红(H&E)染色观察结肠组织病理形态损伤情况,采用免疫组化方法检测大鼠结肠组织Cajal间质细胞(ICC)的表达情况,采用蛋白免疫印迹法、实时荧光定量分析法测定大鼠结肠组织中SCF及其受体c-Kit蛋白和mRNA水平。结果模型组较正常组大鼠具有明显的便秘特征,粪便颗粒干、小、少,精神倦怠,结肠组织病理形态损伤明显,SCF/c-Kit蛋白及mRNA水平、ICC的表达量、肠道推进率均降低(P<0.01);阳性药组、首荟通便胶囊高剂量组较模型组大鼠的便秘症状改善,SCF/c-Kit蛋白及mRNA水平、ICC表达量、肠道推进率均升高(P<0.05);首荟通便胶囊高剂量组与阳性药组之间差异无统计学意义(P>0.05)。结论首荟通便胶囊治疗STC效果显著,其机制可能与激活SCF/c-Kit通路,促进结肠组织ICC增殖有关。 展开更多
关键词 首荟通便胶囊 慢性传输型便秘 scf/c-Kit通路 机制研究
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SCF/c-kit信号通路在胃肠动力障碍性疾病中的作用及中医药干预的研究进展
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作者 张晓宁 徐明瑶 谢胜 《实用中医内科杂志》 2025年第5期118-122,共5页
胃肠动力障碍性疾病(disorders of gastrointestinal motility,DGIM)是指因胃肠动力失常引起的以消化道症状为主要临床表现的疾病。DGIM的发病率有逐年升高的趋势,降低人们生活质量的同时,还增加了社会的医疗支出。DGIM的发病机制尚未... 胃肠动力障碍性疾病(disorders of gastrointestinal motility,DGIM)是指因胃肠动力失常引起的以消化道症状为主要临床表现的疾病。DGIM的发病率有逐年升高的趋势,降低人们生活质量的同时,还增加了社会的医疗支出。DGIM的发病机制尚未完全阐明,多项研究表明其发生发展与胃肠道动力异常关系密切。胃肠动力与胃肠道Cajal间质细胞(ICC)密切相关。ICC是胃肠道产生慢波运动的特殊细胞,ICC数量和形态的保持又与SCF/c-kit信号通路密切相关。近年来的研究表明,SCF/c-kit信号通路的异常改变可导致DGIM的发生。目前,DGIM的西医治疗主要为促动力及抑酸等对症治疗,在一定程度上能够缓解DGIM的症状,但常伴有不良反应,使药物的应用范围受限,而大量研究证实,中药、中药单体或成分、复方、中医外治法均可通过调节SCF/c-kit信号通路,有效改善胃肠动力的同时,不良反应较小。虽然中医药通过调控SCF/c-kit信号通路干预DGIM具有西医难以取代的独特优势,但目前中医药调控SCF/c-kit信号通路治疗DGIM系统综述仍较少。文章就SCF/c-kit信号通路与DGIM之间的相关性、中医药调控SCF/c-kit信号通路干预DGIM的作用机制进行系统性总结,旨在为临床治疗DGIM及新药研发提供新思考。 展开更多
关键词 scf/c-kit信号通路 胃肠道动力障碍性疾病 中医药 作用机制 研究进展
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基于SCF/c-kit信号通路探讨半夏调中颗粒对反流性食管炎模型大鼠的影响
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作者 彭洪姣 时昭红 《中国中医药信息杂志》 2025年第10期107-112,共6页
目的探讨半夏调中颗粒治疗反流性食管炎(RE)的作用机制。方法采用前胃结扎结合外置幽门部分结扎术建立RE大鼠模型。将大鼠随机分为假手术组、模型组、阳性对照组和半夏调中颗粒低、中、高剂量组,造模结束后7 d,各组分别予相应干预,连续1... 目的探讨半夏调中颗粒治疗反流性食管炎(RE)的作用机制。方法采用前胃结扎结合外置幽门部分结扎术建立RE大鼠模型。将大鼠随机分为假手术组、模型组、阳性对照组和半夏调中颗粒低、中、高剂量组,造模结束后7 d,各组分别予相应干预,连续14 d。HE染色观察食管组织形态,免疫荧光染色观察食管组织Cajal间质细胞(ICC)表达,RT-qPCR检测食管组织干细胞因子(SCF)、c-kit mRNA表达,Western blot检测食管组织SCF、c-kit蛋白表达,ELISA检测血清胃动素(MTL)、胃泌素(GAS)、血管活性肠肽(VIP)含量。结果与假手术组比较,模型组大鼠食管鳞状上皮细胞间隙增宽,基底细胞增生,黏膜层见大量炎性细胞浸润,食管组织ICC数量显著减少(P<0.01),SCF、c-kit mRNA和蛋白显著升高(P<0.01);血清MTL、GAS含量显著降低(P<0.01),VIP含量显著升高(P<0.01)。与模型组比较,阳性对照组和半夏调中颗粒各剂量组食管组织基底细胞增生及炎性细胞浸润不同程度减轻,阳性对照组及半夏调中颗粒中、高剂量组ICC数量显著增加(P<0.01),SCF、c-kit mRNA和蛋白显著降低(P<0.01);血清MLT、GAS含量显著升高(P<0.01),VIP含量显著降低(P<0.01)。结论半夏调中颗粒可通过调节胃肠激素,激活SCF/c-kit信号通路,维持ICC功能,从而调节食管动力,改善食管黏膜炎症,发挥治疗RE作用。 展开更多
关键词 反流性食管炎 半夏调中颗粒 CAJAL间质细胞 scf/c-kit信号通路 胃肠激素 食管动力 大鼠
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Genome-wide analysis of the S-phase kinase-association protein1(ClSKP1) family and the role of S-RNase targeting by an SCF(Cullin1-SKP1-F-box) complex in the self-incompatibility of‘Xiangshui' lemon 被引量:1
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作者 Yuze Li Wei Lin +9 位作者 Jiawei Zhu Moying Lan Cong Luo Yili Zhang Rongzhen Liang Liming Xia Wangli Hu Xiao Mo Guixiang Huang Xinhua He 《Horticultural Plant Journal》 2025年第2期593-607,共15页
The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the ... The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the SCF complex and degraded by the 26S protease accounts for the bulk of the available self-incompatibility studies.In this study,15 ClSKP1s from the‘Xiangshui'lemon genome and ubiquitome exist in the same SKP1 conserved domain(CD)as SKP1s in other species.The q PCR results showed that SKP1-6 and SKP1-14 have tissue expression patterns specific for expression in pollen.In addition,SKP1-6 and SKP1-14 in the stigma,style and ovary were significantly upregulated after self-pollination compared to those after cross-pollination.A subcellular location showed that SKP1-6 and SKP1-14 were located in the nucleus.In addition,yeast two-hybrid(Y2H)assays,bimolecular fluorescence complementation(BiFC)and luciferase complementation imaging(LCI)assays showed that SKP1-6 interacted with F-box1,F-box33,F-box34,F-box17,F-box19,Cullin1-2 and 26S proteasome subunit 4 homolog A(26S PS4HA).SKP1-14 interacted with F-box17,F-box19,F-box35,Cullin1-2 and 26S PS4HA.The interaction of Cullin1-2 and the F-box with SKP1 as a bridge was verified by a yeast three-hybrid experiment.The ability of S3-RNase to inhibit pollen and pollen tube growth and development was assessed using in vitro pollen co-culture experiments with recombinant S3-RNase proteins.Overall,this study provides important experimental evidence and theoretical basis for understanding the mechanism of self-incompatibility in plants by revealing the key role of the SCF complex in‘Xiangshui'lemon,which is bridged by ClSKP1-6,in self-incompatibility.The results of this study are of great significance for the future indepth exploration of the molecular mechanism of the SCF complex and its wide application in the self-incompatibility of plants. 展开更多
关键词 LEMON SKP1 scf SELF-INCOMPATIBILITY Expression analysis Functional analysis
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SFPBL:Soft Filter Pruning Based on Logistic Growth Differential Equation for Neural Network 被引量:1
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作者 Can Hu Shanqing Zhang +2 位作者 Kewei Tao Gaoming Yang Li Li 《Computers, Materials & Continua》 2025年第3期4913-4930,共18页
The surge of large-scale models in recent years has led to breakthroughs in numerous fields,but it has also introduced higher computational costs and more complex network architectures.These increasingly large and int... The surge of large-scale models in recent years has led to breakthroughs in numerous fields,but it has also introduced higher computational costs and more complex network architectures.These increasingly large and intricate networks pose challenges for deployment and execution while also exacerbating the issue of network over-parameterization.To address this issue,various network compression techniques have been developed,such as network pruning.A typical pruning algorithm follows a three-step pipeline involving training,pruning,and retraining.Existing methods often directly set the pruned filters to zero during retraining,significantly reducing the parameter space.However,this direct pruning strategy frequently results in irreversible information loss.In the early stages of training,a network still contains much uncertainty,and evaluating filter importance may not be sufficiently rigorous.To manage the pruning process effectively,this paper proposes a flexible neural network pruning algorithm based on the logistic growth differential equation,considering the characteristics of network training.Unlike other pruning algorithms that directly reduce filter weights,this algorithm introduces a three-stage adaptive weight decay strategy inspired by the logistic growth differential equation.It employs a gentle decay rate in the initial training stage,a rapid decay rate during the intermediate stage,and a slower decay rate in the network convergence stage.Additionally,the decay rate is adjusted adaptively based on the filter weights at each stage.By controlling the adaptive decay rate at each stage,the pruning of neural network filters can be effectively managed.In experiments conducted on the CIFAR-10 and ILSVRC-2012 datasets,the pruning of neural networks significantly reduces the floating-point operations while maintaining the same pruning rate.Specifically,when implementing a 30%pruning rate on the ResNet-110 network,the pruned neural network not only decreases floating-point operations by 40.8%but also enhances the classification accuracy by 0.49%compared to the original network. 展开更多
关键词 filter pruning channel pruning CNN complexity deep neural networks filtering theory logistic model
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A Compact Dual-Band Bandpass Filter with Large Frequency Ratio Based on Ultra-Wide-Stopband Bandpass Filter 被引量:1
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作者 Tang Weisheng Zheng Shaoyong Pan Yongmei 《China Communications》 2025年第7期156-169,共14页
With the rapid development of wireless techniques,the bandpass filter(BPF)is required to cover microwave and millimeter-wave frequency bands simultaneously with good mid-band suppression.However,it is difficult to imp... With the rapid development of wireless techniques,the bandpass filter(BPF)is required to cover microwave and millimeter-wave frequency bands simultaneously with good mid-band suppression.However,it is difficult to implement such BPF due to the large frequency ratio and wideband rejection.This paper presents a superior method to realize a dual-band BPF with a large frequency ratio maintaining compact size and low design complexity.This is contributed by an ultra-wide stopband BPF with inherent discriminating excited degree at spurious frequencies.By properly arranging the feeding position and electrical length ratio of stepped impedance resonator(SIR),the excited degree at specific spurious frequencies can be flexibly adjusted to achieve desired suppression level without affecting characteristics at the fundamental passband.For validation,two BPFs were simulated,fabricated and measured,exhibiting suppression levels of 20.3 dB and 35 dB up to 18f0 and 10.53f0 respectively.Based on this,a dual-band BPF with a large frequency ratio can be easily constructed.For demonstration,a dual-band BPF operating at 3.55 GHz and 43.15 GHz is implemented.A frequency ratio up to 12.15 and mid-band suppression level better than 28 dB had been achieved.Advantages of compactness,simplicity and excellent performance of the proposed work can be observed. 展开更多
关键词 bandpass filter dual-band filter large frequency ratio MILLIMETER-WAVE stepped impedance resonator ultra-wide stopband
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