Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It a...Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It also reduces scar tissue formation and promotes axonal regeneration after spinal cord injury.The aim of the present study was to investigate the effect and mechanism of the microtubule-stabilizing reagent epothilone B in decreasing fibrotic scarring through its action on pericytes after spinal cord injury.A rat model of spinal cord injury was established via dorsal complete transection at the T10 vertebra.The rats received an intraperitoneal injection of epothilone B（0.75 mg/kg） at 1 and 15 days post-injury in the epothilone B group or normal saline in the vehicle group.Neuron-glial antigen 2,platelet-derived growth factor receptor β,and fibronectin protein expression were dramatically lower in the epothilone B group than in the vehicle group,but β-tubulin protein expression was greater.Glial fibrillary acidic protein at the injury site was not affected by epothilone B treatment.The Basso,Beattie,and Bresnahan locomotor scores were significantly higher in the epothilone B group than in the vehicle group.The results of this study demonstrated that epothilone B reduced the number of pericytes,inhibited extracellular matrix formation,and suppressed scar formation after spinal cord injury.展开更多
Liver fibrosis is the formation of a fibrous scar due to chronic liver disease including viral hepatitis,alcohol and non-alcoholic fatty liver disease.Without treatment,it will develop into cirrhosis and hepatocellula...Liver fibrosis is the formation of a fibrous scar due to chronic liver disease including viral hepatitis,alcohol and non-alcoholic fatty liver disease.Without treatment,it will develop into cirrhosis and hepatocellular carcinoma.Up to now,there is no effective way to cure liver fibrosis.Extracellular vesicles(EVs)are biological nanoparticles with potential to be therapeutical agents or delivery tools.A lot of studies have demonstrated the therapeutical effect of EVs on liver fibrosis.In this review,we mainly pay attention to roles of liver non-parenchymal cells in pathology of fibrosis,the basic information about EVs and therapeutical effect on liver fibrosis of EVs when they act on non-parenchymal cells.展开更多
基金supported by a grant from the Science and Technology Developing Program of Shandong Provincial Government of China,No.2010GSF10254a grant from the Medical and Health Science and Technology Plan Project of Shandong Province of China,No.2015WS0504
文摘Scar formation after spinal cord injury is regarded as an obstacle to axonal regeneration and functional recovery.Epothilone B provides moderate microtubule stabilization and is mainly used for anti-tumor therapy.It also reduces scar tissue formation and promotes axonal regeneration after spinal cord injury.The aim of the present study was to investigate the effect and mechanism of the microtubule-stabilizing reagent epothilone B in decreasing fibrotic scarring through its action on pericytes after spinal cord injury.A rat model of spinal cord injury was established via dorsal complete transection at the T10 vertebra.The rats received an intraperitoneal injection of epothilone B（0.75 mg/kg） at 1 and 15 days post-injury in the epothilone B group or normal saline in the vehicle group.Neuron-glial antigen 2,platelet-derived growth factor receptor β,and fibronectin protein expression were dramatically lower in the epothilone B group than in the vehicle group,but β-tubulin protein expression was greater.Glial fibrillary acidic protein at the injury site was not affected by epothilone B treatment.The Basso,Beattie,and Bresnahan locomotor scores were significantly higher in the epothilone B group than in the vehicle group.The results of this study demonstrated that epothilone B reduced the number of pericytes,inhibited extracellular matrix formation,and suppressed scar formation after spinal cord injury.
基金funded by the National Natural Science Foundation of China(81422009,81770560,81970535)was supported by grants from the National Key Research and Development Program of China(2016YFA0102100,2021YFA1100500).
文摘Liver fibrosis is the formation of a fibrous scar due to chronic liver disease including viral hepatitis,alcohol and non-alcoholic fatty liver disease.Without treatment,it will develop into cirrhosis and hepatocellular carcinoma.Up to now,there is no effective way to cure liver fibrosis.Extracellular vesicles(EVs)are biological nanoparticles with potential to be therapeutical agents or delivery tools.A lot of studies have demonstrated the therapeutical effect of EVs on liver fibrosis.In this review,we mainly pay attention to roles of liver non-parenchymal cells in pathology of fibrosis,the basic information about EVs and therapeutical effect on liver fibrosis of EVs when they act on non-parenchymal cells.
