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The Fibered Isomorphism Conjecture for Complex Manifolds
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作者 S.K.ROUSHON 《Acta Mathematica Sinica,English Series》 SCIE CSCD 2007年第4期639-658,共20页
In this paper we show that the Fibered Isomorphism Conjecture of Farrell and Jones, corresponding to the stable topological pseudoisotopy functor, is true for the fundamental groups of a class of complex manifolds. A ... In this paper we show that the Fibered Isomorphism Conjecture of Farrell and Jones, corresponding to the stable topological pseudoisotopy functor, is true for the fundamental groups of a class of complex manifolds. A consequence of this result is that the Whitehead group, reduced projective class groups and the negative K-groups of the fundamental groups of these manifolds vanish whenever the fundamental group is torsion free. We also prove the same results for a class of real manifolds including a large class of 3-manifolds which has a finite sheeted cover fibering over the circle. 展开更多
关键词 Complex projective variety Complex surfaces Whitehead group fibered isomorphism conjecture Negative K-groups
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Differential plasticity of excitatory and inhibitory reticulospinal fibers after spinal cord injury:Implication for recovery
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作者 Rozaria Jeleva Carmen Denecke Muhr +1 位作者 Alina P.Liebisch Florence M.Bareyre 《Neural Regeneration Research》 2026年第5期2011-2020,共10页
The remodeling of axonal connections following injury is an important feature driving functional recovery.The reticulospinal tract is an interesting descending motor tract that contains both excitatory and inhibitory ... The remodeling of axonal connections following injury is an important feature driving functional recovery.The reticulospinal tract is an interesting descending motor tract that contains both excitatory and inhibitory fibers.While the reticulospinal tract has been shown to be particularly prone to axonal growth and plasticity following injuries of the spinal cord,the differential capacities of excitatory and inhibitory fibers for plasticity remain unclear.As adaptive axonal plasticity involves a sophisticated interplay between excitatory and inhibitory input,we investigated in this study the plastic potential of glutamatergic(vGlut2)and GABAergic(vGat)fibers originating from the gigantocellular nucleus and the lateral paragigantocellular nucleus,two nuclei important for locomotor function.Using a combination of viral tracing,chemogenetic silencing,and AI-based kinematic analysis,we investigated plasticity and its impact on functional recovery within the first 3 weeks following injury,a period prone to neuronal remodeling.We demonstrate that,in this time frame,while vGlut2-positive fibers within the gigantocellular and lateral paragigantocellular nuclei rewire significantly following cervical spinal cord injury,vGat-positive fibers are rather unresponsive to injury.We also show that the acute silencing of excitatory axonal fibers which rewire in response to lesions of the spinal cord triggers a worsening of the functional recovery.Using kinematic analysis,we also pinpoint the locomotion features associated with the gigantocellular nucleus or lateral paragigantocellular nucleus during functional recovery.Overall,our study increases the understanding of the role of the gigantocellular and lateral paragigantocellular nuclei during functional recovery following spinal cord injury. 展开更多
关键词 GABAergic(vGat)fibers gait features glutamatergic(vGlut2)fibers PLASTICITY recovery of function reticulospinal tract spinal cord injury
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Influence of ultrasonic agitation on dispersion of fibers in a shell mold for investment casting
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作者 Zhi-cheng Feng Kai Lü +2 位作者 Yan Lu Wen-bo Jin Lei Che 《China Foundry》 2026年第1期108-116,共9页
To develop a suitable production process for fiber reinforced investment casting shell mold,three methods were studied:the traditional method(M_(1)),the method of adding fiber into silica sol with mechanical stirring ... To develop a suitable production process for fiber reinforced investment casting shell mold,three methods were studied:the traditional method(M_(1)),the method of adding fiber into silica sol with mechanical stirring and ultrasonic agitation(M_(2)),and the method of adding fiber into slurry with mechanical stirring and ultrasonic agitation for durations of 3,15,30,and 45 min(M_(3)).The bending strength,high-temperature self-load deformation,and thermal conductivity of the shell molds were investigated.The results reveal that the enhancement of fiber dispersion through ultrasonic agitation improves the comprehensive performance of the shell molds.The maximum green bending strength of the shell mold by M_(2) reaches 3.29 MPa,which is 29% higher than that of the shell mold prepared by M_(1).Moreover,the high-temperature self-load deformation of the shell mold is reduced from 0.62% to 0.44%.In addition,simultaneous ultrasonic agitation and mechanical stirring effectively shorten the slurry preparation time while maintaining comparable levels of fiber dispersion.With the process M_(3)-45 min,the fillers are uniformly dispersed in the slurry,and the fired bending strength and the high-temperature self-load deformation reach 6.25 MPa and 0.41%,respectively.Therefore,the proposed ultrasonic agitation route is promising for the fabrication of fiber-reinforced shell molds with excellent fibers dispersion. 展开更多
关键词 investment casting steel fibers fiber-reinforced shell ultrasonic agitation thermal conductivity
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Thermally Drawn Flexible Fiber Sensors:Principles,Materials,Structures,and Applications
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作者 ZhaoLun Zhang Yuchang Xue +7 位作者 Pengyu Zhang Xiao Yang Xishun Wang Chunyang Wang Haisheng Chen Xinghua Zheng Xin Yin Ting Zhang 《Nano-Micro Letters》 2026年第1期95-129,共35页
Flexible fiber sensors,However,traditional methods face challenges in fabricating low-cost,large-scale fiber sensors.In recent years,the thermal drawing process has rapidly advanced,offering a novel approach to flexib... Flexible fiber sensors,However,traditional methods face challenges in fabricating low-cost,large-scale fiber sensors.In recent years,the thermal drawing process has rapidly advanced,offering a novel approach to flexible fiber sensors.Through the preform-tofiber manufacturing technique,a variety of fiber sensors with complex functionalities spanning from the nanoscale to kilometer scale can be automated in a short time.Examples include temperature,acoustic,mechanical,chemical,biological,optoelectronic,and multifunctional sensors,which operate on diverse sensing principles such as resistance,capacitance,piezoelectricity,triboelectricity,photoelectricity,and thermoelectricity.This review outlines the principles of the thermal drawing process and provides a detailed overview of the latest advancements in various thermally drawn fiber sensors.Finally,the future developments of thermally drawn fiber sensors are discussed. 展开更多
关键词 Thermally drawn fiber sensors Sensing principles Temperature sensors Mechanical sensors Multifunctional sensors
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Optimized fiber allocation for enhanced impact resistance in composites through damage mode suppression
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作者 Noha M.Hassan Zied Bahroun +2 位作者 Mahmoud I.Awad Rami As'ad El-Cheikh Amer Kaiss 《Defence Technology(防务技术)》 2026年第1期316-329,共14页
Variable stiffness composites present a promising solution for mitigating impact loads via varying the fiber volume fraction layer-wise,thereby adjusting the panel's stiffness.Since each layer of the composite may... Variable stiffness composites present a promising solution for mitigating impact loads via varying the fiber volume fraction layer-wise,thereby adjusting the panel's stiffness.Since each layer of the composite may be affected by a different failure mode,the optimal fiber volume fraction to suppress damage initiation and evolution is different across the layers.This research examines how re-allocating the fibers layer-wise enhances the composites'impact resistance.In this study,constant stiffness panels with the same fiber volume fraction throughout the layers are compared to variable stiffness ones by varying volume fraction layer-wise.A method is established that utilizes numerical analysis coupled with optimization techniques to determine the optimal fiber volume fraction in both scenarios.Three different reinforcement fibers(Kevlar,carbon,and glass)embedded in epoxy resin were studied.Panels were manufactured and tested under various loading conditions to validate results.Kevlar reinforcement revealed the highest tensile toughness,followed by carbon and then glass fibers.Varying reinforcement volume fraction significantly influences failure modes.Higher fractions lead to matrix cracking and debonding,while lower fractions result in more fiber breakage.The optimal volume fraction for maximizing fiber breakage energy is around 45%,whereas it is about 90%for matrix cracking and debonding.A drop tower test was used to examine the composite structure's behavior under lowvelocity impact,confirming the superiority of Kevlar-reinforced composites with variable stiffness.Conversely,glass-reinforced composites with constant stiffness revealed the lowest performance with the highest deflection.Across all reinforcement materials,the variable stiffness structure consistently outperformed its constant stiffness counterpart. 展开更多
关键词 Sandwich panel Fiber reinforced plastic composites Finite element analysis Variable stiffness Impact resistance Regression analysis Process optimization
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Boosting the adsorption performance of ethanol on surface chemistry modified activated carbon fiber
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作者 Mengyan Wang Yuxuan Wang +6 位作者 Junhao Wang Yinghui Han Jianxiao Yang Suchan Yang Yuanxun Zhang Peng Huo Xin Zhang 《Journal of Environmental Sciences》 2026年第1期178-186,共9页
As a potential adsorption material,it is still a challenge for activated carbon fiber(ACF)in efficient adsorption of ethanol due to its nonpolar surface,which is mainly emitted from the grain drying industry.This stud... As a potential adsorption material,it is still a challenge for activated carbon fiber(ACF)in efficient adsorption of ethanol due to its nonpolar surface,which is mainly emitted from the grain drying industry.This study prepared surface polarity-modified ACF using the heteroatom doping method.The modified ACF possessed a richer array of strongly polar oxygen/nitrogen-containing functional groups(primarily phenolic hydroxyl and lactone groups),a larger specific surface are1,and a more developed micropore structure.The adsorption capacities of ethanol for O-ACF and N-ACF were 4.110 mmol/g and 1.698 mmol/g,respectively,which were 11.3 times and 4.7 times those of unmodified ACF.This was a significant improvement over our previous work(0.363 mmol/g).The improvement of adsorption capacity for the N-ACF was mainly due to the higher specific surface are1,greater number of micropores(more adsorption sites)and abundant existence of defects,whereas,for O-ACF,the improvement mainly relied on the abundant presence of oxygen-containing functional groups on the surface.However,water had a negative effect on the adsorption of ethanol for the modified ACF due to competitive adsorption and the disappearance of capillary condensation.It was further revealed that the adsorption process of ethanol and water was quite different.It obeyed the linear driving force(LDF)model for ethanol adsorption,however,the intraparticle diffusion(IPD)model for water adsorption. 展开更多
关键词 Activated carbon fiber(ACF) Heteroatom doping Surface polarity-modified Polar functional groups Microporous filling Linear driving force(LDF) In-particle diffusion(IPD)
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A Cross-Band Quantum Light Source Based on Spontaneous Four-Wave Mixing in a Shallow-Ridge Silicon Waveguide
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作者 Yijia Wang Qirui Ren +2 位作者 Zhanping Jin Yidong Huang Wei Zhang 《Chinese Physics Letters》 2026年第1期64-70,共7页
To fully utilize the resources provided by optical fiber networks,a cross-band quantum light source generating photon pairs,where one photon in a pair is at C band and the other is at O band,is proposed in this work.T... To fully utilize the resources provided by optical fiber networks,a cross-band quantum light source generating photon pairs,where one photon in a pair is at C band and the other is at O band,is proposed in this work.This source is based on spontaneous four-wave mixing(SFWM)in a piece of shallow-ridge silicon waveguide.Theoretical analysis shows that the waveguide dispersion could be tailored by adjusting the ridge width,enabling broadband photon pair generation by SFWM across C band and O band.The spontaneous Raman scattering(SpRS)in silicon waveguides is also investigated experimentally.It shows that there are two regions in the spectrum of generated photons from SpRS,which could be used to achieve cross-band photon pair generation.A chip of shallow-ridge silicon waveguide samples with different ridge widths has been fabricated,through which cross-band photon pair generation is demonstrated experimentally.The experimental results show that the source can be achieved using dispersion-optimized shallow-ridge silicon waveguides.This cross-band quantum light source provides a way to develop new fiber-based quantum communication functions utilizing both C band and O band and extends applications of quantum networks. 展开更多
关键词 photon pair generation shallow ridge silicon waveguide spontaneous four wave mixing optical fiber networks adjusting ridge widthenabling cross band quantum light source broadband photon pair generation waveguide dispersion
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ENVIRONMENTAL BENEFIT OF TWO-LAYER STEEL FIBERED HIGH-PERFORMANCE CONCRETE BEAMS
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作者 Svetlana Pushkar Yuri Ribakov 《Journal of Green Building》 2021年第3期237-250,共14页
This study evaluated Life-Cycle Assessment(LCA)of two different designs of high-performance concrete beam:(1)a single-layer beam(SLB)that consisted of steelfibered high-strength concrete in both the compression and ten... This study evaluated Life-Cycle Assessment(LCA)of two different designs of high-performance concrete beam:(1)a single-layer beam(SLB)that consisted of steelfibered high-strength concrete in both the compression and tensile zones and(2)a two-layer beam(TLB)that consisted of steelfibered high-strength concrete and normal-strength concrete in the compression and tensile zones,respectively.The SLB and steelfibered high-strength concrete layer of the TLB were of the same concrete class C70/85.LCAs of the SLB and TLB were conducted using the ReCiPe2016 mid-point and endpoint-single-score methods.The difference between the two endpoint-single-score results was evaluated using a two-stage nested analysis of variance.The ReCiPe2016 midpoint results showed that replacing the SLB with the TLB reduces the environmental impact of global warming potential,terrestrial ecotoxicity,water consumption,and scarcity of fossil resources by 15%,17%,11%,and 17%,respec-tively.The ReCiPe2016 endpoint-single-score results showed that the environmental damage from the TLB compared to the SLB was statistically reduced(p=0.0256).Therefore,considering two different designs of steelfibered high-strength concrete beams,the TLB design was found environmentally preferable to SLB design on both,midpoint and endpoint-single-score evaluations. 展开更多
关键词 Steel fibered high-strength concrete two-layer beam design method life-cycle assessment(LCA) two-stage nested ANOVA
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Gamma(ɣ)‑MnO_(2)/rGO Fibered Cathode Fabrication from Wet Spinning and Dip Coating Techniques for Cable‑Shaped Zn‑Ion Batteries 被引量:4
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作者 Nakarin Subjalearndee Nanfei He +6 位作者 Hui Cheng Panpanat Tesatchabut Priew Eiamlamai Pimpa Limthongkul Varol Intasanta Wei Gao Xiangwu Zhang 《Advanced Fiber Materials》 SCIE CAS 2022年第3期457-474,共18页
Cable/fber-shaped Zn-ion batteries are designed to power wearable electronics that require high fexibility to operate on human body.However,one of technical challenges of these devices is the complexity and high cost ... Cable/fber-shaped Zn-ion batteries are designed to power wearable electronics that require high fexibility to operate on human body.However,one of technical challenges of these devices is the complexity and high cost for manufacturing fbered cathode.In this work,we demonstrated gamma manganese oxide(ɣ-MnO_(2))/reduced graphene oxide(rGO)fbered cathode fabrication using facile and cost-efective fber production and active material coating techniques.Specifcally,rGO fbers were fabricated via wet spinning,followed by chemical reduction with hydroiodic acid(HI).The synthesized rGO fber bundle was then dip-coated with a mixture ofɣ-MnO_(2),carbon black or multi-walled carbon nanotubes,and xanthan gum or polyvinylidene fuoride binder to obtainɣ-MnO_(2)/rGO fbered cathode.We studied the efect of binders and conductive materials on physical properties and electrochemical performance of the fbered cathode.It was found that hydrophobic binder had more benefts than hydrophilic binder by providing higher active material loading,better coating layer homogeneity,and more stable electrochemical performance.Cable-shaped Zn-ion batteries(CSZIBs)were then assembled by using theɣ-MnO_(2)/rGO fbered cathode,Zn wire anode,and xanthan gum polymeric gel electrolyte with 2 M ZnSO_(4) and 0.2 M MnSO_(4) salts without a separator.We investigated the battery assembling procedure on a glass slide(prototype ZIB)and in a plastic tube(cable-shaped ZIB),and evaluated their electrochemical performance.The CSZIB showed promising maximum capacity of~230 mAh/g with moderate cycling stability(80%capacity retention after 200 cycles)and high fexibility by maintaining the potential after consecutive pressing for 200 times under controlled pressing distance,duration,and testing speed.Finally,we explored ion intercalation behaviours and proposed a H^(+)/Zn^(2+)co-intercalation mechanism in ZIB withɣ-MnO_(2) active material. 展开更多
关键词 Zn-ion battery Wet spinning Dip coating Reduced graphene oxide Manganese dioxide Fiber
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An experimental investigation of the thermal spalling of polypropylene-fibered reactive powder concrete exposed to elevated temperatures 被引量:14
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作者 Yang Ju Li Wang +1 位作者 Hongbin Liu Kaipei Tian 《Science Bulletin》 SCIE EI CAS CSCD 2015年第23期2022-2053,共32页
Polypropylene fibers are embedded to prevent reactive powder concrete (RPC) from spalling failure under high temperatures. This paper probes the influence of embedded fibers at various volumetric dosages on the ther... Polypropylene fibers are embedded to prevent reactive powder concrete (RPC) from spalling failure under high temperatures. This paper probes the influence of embedded fibers at various volumetric dosages on the thermomechanical properties of polypropylene-fibered reactive powder concrete (PPRPC) exposed to high tem- peratures up to 350 ℃ and on the spalling performance and characteristics up to 600 ℃. The thermomechanical prop- erties include the characteristic temperature for spalling, and residual strengths, such as the compressive strength, split tensile strength, and flexural tensile strength. A high- definition charge-coupled device camera and scanning electron microscope technology were employed to capture the spalling processes and to detect the microstructural changes in the materials with various fiber dosages. To understand and characterize the mechanism by which polypropylene fibers influence the thermal spalling of RPC, a numerical model to determine the moisture migration and vapor pressure transmission during spalling was developed in this paper. It showed that there was an optimal volu- metric dosage of fibers to prevent PPRPC from explosive spalling. The relationships between the mechanical char- acteristics of PPRPC and the fiber dosages were derived based on experimental data. 展开更多
关键词 Polypropylene reactive powder concrete(PPRPC) · Thermal spalling · Vapor pressuremechanism · Polypropylene fibers ·Elevated temperatures
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禽腺病毒Fiber2蛋白抗原制备2种新型疫苗及保护效果试验
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作者 岳筠 刘霞 +3 位作者 胡茜 许力文 尹德晶 程振涛 《贵州畜牧兽医》 2025年第6期27-31,共5页
为提高禽腺病毒4型(FAdV-4)疫苗的免疫效果,以FAdV-4贵州分离株的纤突蛋白(Fiber2)为抗原,通过间接免疫荧光检测pVAX1-Fiber2重组质粒在LMH细胞内的表达情况,以此构建重组质粒,制备DNA疫苗;通过pColdⅠ载体对Fiber2进行原核表达,构建原... 为提高禽腺病毒4型(FAdV-4)疫苗的免疫效果,以FAdV-4贵州分离株的纤突蛋白(Fiber2)为抗原,通过间接免疫荧光检测pVAX1-Fiber2重组质粒在LMH细胞内的表达情况,以此构建重组质粒,制备DNA疫苗;通过pColdⅠ载体对Fiber2进行原核表达,构建原核表达载体,制备亚单位疫苗,并对2种新型疫苗与灭活疫苗的攻毒保护效果进行比较。结果:pVAX1-Fiber2重组质粒可在LMH细胞内有效表达Fiber2蛋白,原核表达的Fiber2重组蛋白具有良好的免疫原性;Fiber2亚单位疫苗、Fiber2 DNA疫苗、灭活疫苗的保护率分别为98%、58%、96%。组织病理学分析显示,3种疫苗均可减轻FAdV-4感染的病理变化程度,其中Fiber2亚单位疫苗与灭活疫苗的效果更好。结论:Fiber2亚单位疫苗具有良好的攻毒保护效果,可作为防控FAdV-4的候选疫苗。 展开更多
关键词 禽腺病毒4型 Fiber2蛋白 DNA疫苗 亚单位疫苗 保护效果
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含有FAdV-4 Fiber 2 B细胞表位的IBV S1融合蛋白的表达及免疫原性
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作者 高亚茹 王泱 +8 位作者 张瀚文 刘琳 曹晓阳 刘静瑞 李冰洁 常晶晶 宋亚鹏 高文明 李新生 《中国兽医学报》 北大核心 2025年第8期1616-1623,共8页
为验证禽腺病毒4(FAdV-4)WZ株Fiber 2 B细胞表位Pep1和Pep4可作为多价表位疫苗的候选表位,将表位与鸡传染性支气管炎病毒M41株S1蛋白基因以不同模式进行串联,构建重组融合质粒,并在大肠杆菌BL21(DE3)中表达。经Western blot和ELISA试验... 为验证禽腺病毒4(FAdV-4)WZ株Fiber 2 B细胞表位Pep1和Pep4可作为多价表位疫苗的候选表位,将表位与鸡传染性支气管炎病毒M41株S1蛋白基因以不同模式进行串联,构建重组融合质粒,并在大肠杆菌BL21(DE3)中表达。经Western blot和ELISA试验证实,表达获得的4个融合蛋白均能特异性地与抗M41全病毒血清和WZ株抗Fiber 2-knob蛋白血清发生反应。纯化后免疫BALB/c小鼠,在小鼠血清中可检测出抗肽表位的特异性抗体,其中Pep4表位比Pep1表位能诱导更强的免疫反应。当Pep1分别连接在融合蛋白的氨基端和羧基端时,均能使免疫动物产生相同水平的抗Pep1抗体,而将Pep4连接在融合蛋白羧基端时,免疫动物产生的抗Pep4特异性抗体水平更高。结果表明,具有反应原性的WZ株Fiber 2的B细胞表位Pep1和Pep4在与蛋白偶联时形成的融合蛋白可以使Pep1和Pep4获得免疫原性,且不影响载体蛋白的抗原性,这为FAdV-4多价表位疫苗的设计和研发提供了技术支撑和参考依据。 展开更多
关键词 B细胞表位 FAdV-4 Fiber 2 传染性支气管炎 融合蛋白
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禽4型腺病毒单克隆抗体的制备、鉴定与初步应用
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作者 张宇 程凡玉 +7 位作者 俞赵荣 邵颖 魏宁波 陈芳芳 王振宇 宋祥军 涂健 祁克宗 《畜牧兽医学报》 北大核心 2025年第5期2364-2371,共8页
本研究旨在制备禽4型腺病毒(fowl adenovirus type 4,FAdV-4)的单克隆抗体,鉴定其特异性结合位点,并评估其在免疫组化(immunohistochemistry,IHC)和免疫沉淀反应中的应用价值。使用纯化的FAdV-4-AH-F41株全病毒免疫小鼠,通过间接酶联免... 本研究旨在制备禽4型腺病毒(fowl adenovirus type 4,FAdV-4)的单克隆抗体,鉴定其特异性结合位点,并评估其在免疫组化(immunohistochemistry,IHC)和免疫沉淀反应中的应用价值。使用纯化的FAdV-4-AH-F41株全病毒免疫小鼠,通过间接酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法筛选出一株阳性杂交瘤细胞并进行亚克隆,并对所制备的单克隆抗体进行效价检测。利用间接免疫荧光(indirect immunofluorescence assay,IFA)与蛋白免疫印迹(Western blot)方法鉴定单克隆抗体生物学特性,构建了pCold TF-Hexon、pET-32a(+)-Fiber1、pCold TF-Penton、pCold TF-Fiber2的原核表达载体,并通过Western blot初步鉴定所得单克隆抗体的特异性结合位点,在检测出抗体的亚型后,将其初步应用于IHC和免疫沉淀试验。结果表明本试验获得1株稳定分泌抗Fiber1蛋白的单克隆抗体,并将其命名为5C7。该抗体效价为1∶102400,亚型为IgG-2b,表现出良好的生物学特性。在IHC中,能够观察到明显的病变特征;在免疫沉淀试验中,5C7可作为捕获抗体,与病毒感染细胞中的Fiber1蛋白特异性结合。结果提示,本试验通过全病毒粒子免疫小鼠所制备的单克隆抗体能够特异性识别Fiber1蛋白,并在IHC和免疫沉淀反应中显示出良好的应用前景,为FAdV-4实验室病理诊断方法的建立和Fiber1蛋白的功能研究奠定了基础。 展开更多
关键词 血清4型禽腺病毒 单克隆抗体 抗体鉴定 Fiber1蛋白 IHC 免疫共沉淀
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可表达禽腺病毒Fiber2基因的重组马立克病病毒疫苗株的构建
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作者 张子博 陈运通 +12 位作者 范文瑞 吴龙波 李雨萌 郭茹 张艳萍 刘长军 祁小乐 刘永振 王素艳 崔红玉 段雨路 李留安 高玉龙 《中国兽医科学》 北大核心 2025年第7期884-890,共7页
为研制禽类肝炎-心包积液综合征(HHS)重组马立克病病毒(MDV)活载体疫苗,通过PCR扩增血清4型禽腺病毒(FAdV-4)强毒株Fiber2基因并克隆入pcDNA3.1载体,构建重组真核表达质粒pSV40-Fiber2,然后通过PCR扩增和同源重组构建表达黏粒pMS-Fiber2... 为研制禽类肝炎-心包积液综合征(HHS)重组马立克病病毒(MDV)活载体疫苗,通过PCR扩增血清4型禽腺病毒(FAdV-4)强毒株Fiber2基因并克隆入pcDNA3.1载体,构建重组真核表达质粒pSV40-Fiber2,然后通过PCR扩增和同源重组构建表达黏粒pMS-Fiber2,与其他4个相互重叠并覆盖MDV全基因组的黏粒共同转染至鸡胚成纤维细胞(CEF)拯救病毒rMDV-Fiber2,在CEF中传代后用PCR、间接免疫荧光试验和Western-blot进行鉴定,并绘制体外生长曲线,分析其体外复制特性。结果表明,rMDV-Fiber2能够稳定表达Fiber2蛋白,rMDV-Fiber2在CEF中的复制能力与亲本病毒rMSΔmeq无明显差异;攻毒保护试验结果表明,重组病毒rMDV-Fiber2免疫后鸡能够对FAdV-4强毒株感染有80%存活率。结论,构建成功的重组病毒rMDV-Fiber2与亲本株的复制力相似,为研制HHS重组MDV活载体疫苗奠定了基础。 展开更多
关键词 血清4型禽腺病毒 重组马立克病病毒活载体疫苗 Fiber2
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A novel flexible nerve guidance conduit promotes nerve regeneration while providing excellent mechanical properties 被引量:1
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作者 Tong Li Quhan Cheng +11 位作者 Jingai Zhang Boxin Liu Yu Shi Haoxue Wang Lijie Huang Su Zhang Ruixin Zhang Song Wang Guangxu Lu Peifu Tang Zhongyang Liu Kai Wang 《Neural Regeneration Research》 SCIE CAS 2025年第7期2084-2094,共11页
Autografting is the gold standard for surgical repair of nerve defects>5 mm in length;however,autografting is associated with potential complications at the nerve donor site.As an alternative,nerve guidance conduit... Autografting is the gold standard for surgical repair of nerve defects>5 mm in length;however,autografting is associated with potential complications at the nerve donor site.As an alternative,nerve guidance conduits may be used.The ideal conduit should be flexible,resistant to kinks and lumen collapse,and provide physical cues to guide nerve regeneration.We designed a novel flexible conduit using electrospinning technology to create fibers on the innermost surface of the nerve guidance conduit and employed melt spinning to align them.Subsequently,we prepared disordered electrospun fibers outside the aligned fibers and helical melt-spun fibers on the outer wall of the electrospun fiber lumen.The presence of aligned fibers on the inner surface can promote the extension of nerve cells along the fibers.The helical melt-spun fibers on the outer surface can enhance resistance to kinking and compression and provide stability.Our novel conduit promoted nerve regeneration and functional recovery in a rat sciatic nerve defect model,suggesting that it has potential for clinical use in human nerve injuries. 展开更多
关键词 aligned fibers anti-kinking helical fibers nerve guidance conduit nerve regeneration peripheral nerve injury topological guidance
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改进静电植绒法制备沥青基碳纤维垂直排列高导热热界面材料
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作者 武美霞 欧阳江坤 +3 位作者 侯梦迪 洪建婷 丁鹏 王金合 《上海大学学报(自然科学版)》 北大核心 2025年第1期105-114,共10页
利用二次静电植绒和底膜收缩的方法,成功制备致密垂直排列的沥青基碳纤维/硅胶复合热界面材料.研究了改进静电植绒法对导热材料的密度、垂直度、导热系数及传热效果的影响.结果显示,在相同植绒时间下,二次植绒的沥青基碳纤维植绒密度和... 利用二次静电植绒和底膜收缩的方法,成功制备致密垂直排列的沥青基碳纤维/硅胶复合热界面材料.研究了改进静电植绒法对导热材料的密度、垂直度、导热系数及传热效果的影响.结果显示,在相同植绒时间下,二次植绒的沥青基碳纤维植绒密度和垂直排列状态明显优于一次植绒的沥青基碳纤维.在二次植绒的条件下进行底膜收缩,当底膜收缩率为33%时,热界面材料的垂直导热系数可达27.4 W/(m·K).所制备材料展现出良好的垂直导热性能,所采用的制备工艺具有良好的工业放大可能性,具有在电子芯片散热领域发展的潜力. 展开更多
关键词 改进静电植绒 热界面材料 沥青基碳纤维 (pitch-based CARBON FIBER PCF) 垂直排列
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基于EFBG的海水入侵地下水盐度监测模拟试验研究
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作者 方锦辉 郭君仪 +3 位作者 姜洪涛 施斌 孙梦雅 魏广庆 《工程地质学报》 北大核心 2025年第3期1206-1215,共10页
海水入侵是沿海地区不可忽视的重大灾害,将导致水质恶化、土壤盐渍化等问题,因此,地下水盐度的原位及实时监测具有重要意义。本文提出了一种基于FBG(Fiber Bragg Grating)滤波特性的盐度传感器。采用化学腐蚀法腐蚀光纤包层,使FBG对外... 海水入侵是沿海地区不可忽视的重大灾害,将导致水质恶化、土壤盐渍化等问题,因此,地下水盐度的原位及实时监测具有重要意义。本文提出了一种基于FBG(Fiber Bragg Grating)滤波特性的盐度传感器。采用化学腐蚀法腐蚀光纤包层,使FBG对外界折射率敏感,从而实现盐度传感。通过MATLAB软件模拟了不同腐蚀程度的FBG的中心波长与盐度的关系,验证了该方法在理论上的可行性。同时对EFBG(Etched Fiber Bragg Grating)分别进行温度和盐度灵敏度的测试,并引入一根未腐蚀的FBG进行温度补偿,实现盐度和温度的同时测量。试验结果表明:不同腐蚀程度的EFBG的波长漂移量与盐度均成线性关系,在一定范围内腐蚀程度越高,盐度灵敏度系数越大,最大可达29.432 pm/%,证明了EFBG对海水盐度测量具有一定可行性。最后提出了一种适用于地下水盐度原位监测的封装方法,为沿海地区海水入侵的研究提供了一种新的测量手段。 展开更多
关键词 EFBG(Etched fiber Bragg grating) 海水盐度 模拟试验 可行性
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IoT Empowered Early Warning of Transmission Line Galloping Based on Integrated Optical Fiber Sensing and Weather Forecast Time Series Data 被引量:1
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作者 Zhe Li Yun Liang +1 位作者 Jinyu Wang Yang Gao 《Computers, Materials & Continua》 SCIE EI 2025年第1期1171-1192,共22页
Iced transmission line galloping poses a significant threat to the safety and reliability of power systems,leading directly to line tripping,disconnections,and power outages.Existing early warning methods of iced tran... Iced transmission line galloping poses a significant threat to the safety and reliability of power systems,leading directly to line tripping,disconnections,and power outages.Existing early warning methods of iced transmission line galloping suffer from issues such as reliance on a single data source,neglect of irregular time series,and lack of attention-based closed-loop feedback,resulting in high rates of missed and false alarms.To address these challenges,we propose an Internet of Things(IoT)empowered early warning method of transmission line galloping that integrates time series data from optical fiber sensing and weather forecast.Initially,the method applies a primary adaptive weighted fusion to the IoT empowered optical fiber real-time sensing data and weather forecast data,followed by a secondary fusion based on a Back Propagation(BP)neural network,and uses the K-medoids algorithm for clustering the fused data.Furthermore,an adaptive irregular time series perception adjustment module is introduced into the traditional Gated Recurrent Unit(GRU)network,and closed-loop feedback based on attentionmechanism is employed to update network parameters through gradient feedback of the loss function,enabling closed-loop training and time series data prediction of the GRU network model.Subsequently,considering various types of prediction data and the duration of icing,an iced transmission line galloping risk coefficient is established,and warnings are categorized based on this coefficient.Finally,using an IoT-driven realistic dataset of iced transmission line galloping,the effectiveness of the proposed method is validated through multi-dimensional simulation scenarios. 展开更多
关键词 Optical fiber sensing multi-source data fusion early warning of galloping time series data IOT adaptive weighted learning irregular time series perception closed-loop attention mechanism
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Preparation and sensing characteristics of long-period fiber gratings based on periodic microchannels 被引量:1
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作者 SUN Cai LI Yuan-jun +6 位作者 YANG He-er PAN Xue-peng LIU Shan-ren WANG Bo GAO Meng-meng GUO Qi YU Yong-sen 《中国光学(中英文)》 北大核心 2025年第1期198-205,共8页
Long-period fiber gratings have the advantages of small size,corrosion resistance,anti-electro-magnetic interference,and high sensitivity,making them widely used in biomedicine,the power industry,and aerospace.This pa... Long-period fiber gratings have the advantages of small size,corrosion resistance,anti-electro-magnetic interference,and high sensitivity,making them widely used in biomedicine,the power industry,and aerospace.This paper develops a long-period fiber grating sensor based on periodic microchannels.First,a series of linear structures were etched in the cladding of a single-mode fiber by femtosecond laser microma-chining.Then,the laser-modified region was selectively eroded by selective chemical etching to obtain the periodic microchannel structure.Finally,the channels were filled with polydimethylsiloxane(PDMS)to im-prove the spectral quality.The experimental results show that the sensor has good sensitivity in the measure-ment of various parameters such as temperature,stress,refractive index(RI),and bending.It has a temperat-ure sensitivity of−55.19 pm/℃,a strain sensitivity of−3.19 pm/με,a maximum refractive index sensitivity of 540.28 nm/RIU,and a bending sensitivity of 2.65 dB/m^(-1).All of the measurement parameters show good lin-ear responses.The sensor has strong application prospects in the field of precision measurement and sensing. 展开更多
关键词 long-period fiber gratings femtosecond laser micromachining fiber sensors
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Design of high-speed MUTC-PD with electric field regulation layer 被引量:1
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作者 XU Jian-bo LIU Kai +4 位作者 DONG Xiao-wen DUAN Xiao-feng HUANG Yong-qing WANG Qi REN Xiao-min 《中国光学(中英文)》 北大核心 2025年第2期393-400,共8页
This paper proposes a novel modified uni-traveling-carrier photodiode(MUTC-PD)featuring an electric field regulation layer:a p-type doped thin layer inserted behind the PD’s n-doped cliff layer.This electric field re... This paper proposes a novel modified uni-traveling-carrier photodiode(MUTC-PD)featuring an electric field regulation layer:a p-type doped thin layer inserted behind the PD’s n-doped cliff layer.This electric field regulation layer enhances the PD’s performance by not only reducing and smoothing the electric field intensity in the collector layer,allowing photo-generated electrons to transit at peak drift velocity,but also improving the electric field intensity in the depleted absorber layer and optimizing the photo-generated carriers’saturated transit performance.Additionally,the transport characteristics of the peak drift velocity of photogenerated electrons in the device’s collection layer can be used to optimize its parasitic characteristics.The electron’s peak drift velocity compensates for the lost transit time.Thus improving the 3 dB bandwidth of the PD’s photo response.Finally obtains a MUTC-PD with a 3 dB bandwidth of 68 GHz at a responsivity of 0.502 A/W,making it suitable for 100 Gbit/s optical receivers. 展开更多
关键词 peak electron drift velocity transit performance MUTC-PD optical fiber communication optical interconnect
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