为利用原核表达系统表达禽腺病毒血清4型fiber-2蛋白,并评估其免疫原性。从禽腺病毒血清4型病毒中提取fiber-2基因,经密码子优化后构建重组表达载体pET-30a-fiber-2;将所获得的质粒转入原核表达系统,经诱导表达获得fiber-2蛋白;利用获得...为利用原核表达系统表达禽腺病毒血清4型fiber-2蛋白,并评估其免疫原性。从禽腺病毒血清4型病毒中提取fiber-2基因,经密码子优化后构建重组表达载体pET-30a-fiber-2;将所获得的质粒转入原核表达系统,经诱导表达获得fiber-2蛋白;利用获得的fiber-2蛋白制备疫苗,并以7日龄无特定病原(specific pathogen free,SPF)鸡为免疫对象,采用琼脂凝胶免疫扩散试验(agar gel precipitation,AGP)测定抗体效价,并开展攻毒保护试验。结果显示:重组表达载体pET-30a-fiber-2构建成功,fiber-2蛋白为部分可溶性表达;免疫后,SPF鸡体内产生的抗体效价为1∶2~1∶16,且保护效率达到100%。结果表明,该研究成功利用原核表达系统高效表达禽腺病毒血清4型fiber-2蛋白,且该蛋白具有良好的免疫原性,为禽腺病毒相关亚单位疫苗的研发提供新的思路。展开更多
In the face of the increased global campaign to minimize the emission of greenhouse gases and the need for sustainability in manufacturing, there is a great deal of research focusing on environmentally benign and rene...In the face of the increased global campaign to minimize the emission of greenhouse gases and the need for sustainability in manufacturing, there is a great deal of research focusing on environmentally benign and renewable materials as a substitute for synthetic and petroleum-based products. Natural fiber-reinforced polymeric composites have recently been proposed as a viable alternative to synthetic materials. The current work investigates the suitability of coconut fiber-reinforced polypropylene as a structural material. The coconut fiber-reinforced polypropylene composites were developed. Samples of coconut fiber/polypropylene (PP) composites were prepared using Fused Filament Fabrication (FFF). Tests were then conducted on the mechanical properties of the composites for different proportions of coconut fibers. The results obtained indicate that the composites loaded with 2 wt% exhibited the highest tensile and flexural strength, while the ones loaded with 3 wt% had the highest compression strength. The ultimate tensile and flexural strength at 2 wt% were determined to be 34.13 MPa and 70.47 MPa respectively. The compression strength at 3 wt% was found to be 37.88 MPa. Compared to pure polypropylene, the addition of coconut fibers increased the tensile, flexural, and compression strength of the composite. In the study, an artificial neural network model was proposed to predict the mechanical properties of polymeric composites based on the proportion of fibers. The model was found to predict data with high accuracy.展开更多
为评价Ⅰ群禽腺病毒(4型)Fiber-2重组蛋白亚单位疫苗的免疫保护效果,本研究利用FAV-HN株Fiber-2基因构建pET-30a-Fiber-2重组质粒,转化至BL21感受态诱导表达Fiber-2重组蛋白;Fiber-2蛋白纯化后制备不同抗原含量的亚单位疫苗,免疫SPF鸡...为评价Ⅰ群禽腺病毒(4型)Fiber-2重组蛋白亚单位疫苗的免疫保护效果,本研究利用FAV-HN株Fiber-2基因构建pET-30a-Fiber-2重组质粒,转化至BL21感受态诱导表达Fiber-2重组蛋白;Fiber-2蛋白纯化后制备不同抗原含量的亚单位疫苗,免疫SPF鸡进行免疫效力评价。结果表明,Fiber-2蛋白部分为可溶性表达,纯化后纯度约90%,AGP效价达1∶64。亚单位疫苗抗原含量AGP效价不低于1∶2时,SPF鸡免疫后第21 d AGP抗体10/10阳性,可抵抗Ⅰ群禽腺病毒(4型)强毒株的攻击,提供100%的免疫攻毒保护。展开更多
To understand the enhancing effect and fiber-reinforced mechanism of composite fibers reinforced cement concrete, the influences of composite fibers on micro-cracks and the distribution of composite fibers were evalua...To understand the enhancing effect and fiber-reinforced mechanism of composite fibers reinforced cement concrete, the influences of composite fibers on micro-cracks and the distribution of composite fibers were evaluated by optical electron micrometer(OEM) and scanning electron microscope(SEM). Three kinds of fiber, such as polyacrylonitrile-based carbon fiber, basalt fiber, and glass fiber, were used in the composite fibers reinforced cement concrete. The composite fibers could form a stable structure in concrete after the liquid-phase coupling treatment, gas-liquid double-effect treatment, and inert atmosphere drying. The mechanical properties of composite fibers reinforced concrete(CFRC) were studied by universal test machine(UTM). Moreover, the effect of composite fibers on concrete was analyzed based on the toughness index and residual strength index. The results demonstrated that the composite fibers could improve the mechanical properties of concrete, while the excessive amount of composite fibers had an adverse effect on the mechanical properties of concrete. The composite fibers could significantly improve the toughness index of CFRC, and the increment rate is more than 30%. The composite fibers could form a mesh structure, which could promote the stability of concrete and guarantee the excellent mechanical properties.展开更多
文摘为利用原核表达系统表达禽腺病毒血清4型fiber-2蛋白,并评估其免疫原性。从禽腺病毒血清4型病毒中提取fiber-2基因,经密码子优化后构建重组表达载体pET-30a-fiber-2;将所获得的质粒转入原核表达系统,经诱导表达获得fiber-2蛋白;利用获得的fiber-2蛋白制备疫苗,并以7日龄无特定病原(specific pathogen free,SPF)鸡为免疫对象,采用琼脂凝胶免疫扩散试验(agar gel precipitation,AGP)测定抗体效价,并开展攻毒保护试验。结果显示:重组表达载体pET-30a-fiber-2构建成功,fiber-2蛋白为部分可溶性表达;免疫后,SPF鸡体内产生的抗体效价为1∶2~1∶16,且保护效率达到100%。结果表明,该研究成功利用原核表达系统高效表达禽腺病毒血清4型fiber-2蛋白,且该蛋白具有良好的免疫原性,为禽腺病毒相关亚单位疫苗的研发提供新的思路。
文摘In the face of the increased global campaign to minimize the emission of greenhouse gases and the need for sustainability in manufacturing, there is a great deal of research focusing on environmentally benign and renewable materials as a substitute for synthetic and petroleum-based products. Natural fiber-reinforced polymeric composites have recently been proposed as a viable alternative to synthetic materials. The current work investigates the suitability of coconut fiber-reinforced polypropylene as a structural material. The coconut fiber-reinforced polypropylene composites were developed. Samples of coconut fiber/polypropylene (PP) composites were prepared using Fused Filament Fabrication (FFF). Tests were then conducted on the mechanical properties of the composites for different proportions of coconut fibers. The results obtained indicate that the composites loaded with 2 wt% exhibited the highest tensile and flexural strength, while the ones loaded with 3 wt% had the highest compression strength. The ultimate tensile and flexural strength at 2 wt% were determined to be 34.13 MPa and 70.47 MPa respectively. The compression strength at 3 wt% was found to be 37.88 MPa. Compared to pure polypropylene, the addition of coconut fibers increased the tensile, flexural, and compression strength of the composite. In the study, an artificial neural network model was proposed to predict the mechanical properties of polymeric composites based on the proportion of fibers. The model was found to predict data with high accuracy.
文摘为评价Ⅰ群禽腺病毒(4型)Fiber-2重组蛋白亚单位疫苗的免疫保护效果,本研究利用FAV-HN株Fiber-2基因构建pET-30a-Fiber-2重组质粒,转化至BL21感受态诱导表达Fiber-2重组蛋白;Fiber-2蛋白纯化后制备不同抗原含量的亚单位疫苗,免疫SPF鸡进行免疫效力评价。结果表明,Fiber-2蛋白部分为可溶性表达,纯化后纯度约90%,AGP效价达1∶64。亚单位疫苗抗原含量AGP效价不低于1∶2时,SPF鸡免疫后第21 d AGP抗体10/10阳性,可抵抗Ⅰ群禽腺病毒(4型)强毒株的攻击,提供100%的免疫攻毒保护。
基金Funded by the National Natural Science Foundation of China(No.51778479).
文摘To understand the enhancing effect and fiber-reinforced mechanism of composite fibers reinforced cement concrete, the influences of composite fibers on micro-cracks and the distribution of composite fibers were evaluated by optical electron micrometer(OEM) and scanning electron microscope(SEM). Three kinds of fiber, such as polyacrylonitrile-based carbon fiber, basalt fiber, and glass fiber, were used in the composite fibers reinforced cement concrete. The composite fibers could form a stable structure in concrete after the liquid-phase coupling treatment, gas-liquid double-effect treatment, and inert atmosphere drying. The mechanical properties of composite fibers reinforced concrete(CFRC) were studied by universal test machine(UTM). Moreover, the effect of composite fibers on concrete was analyzed based on the toughness index and residual strength index. The results demonstrated that the composite fibers could improve the mechanical properties of concrete, while the excessive amount of composite fibers had an adverse effect on the mechanical properties of concrete. The composite fibers could significantly improve the toughness index of CFRC, and the increment rate is more than 30%. The composite fibers could form a mesh structure, which could promote the stability of concrete and guarantee the excellent mechanical properties.
