Introduction:Chikungunya virus(CHIKV),transmitted by Aedes aegypti and Aedes albopictus,causes chikungunya fever.Since its 2005 re-emergence,it has become endemic in 119 countries across Africa,Asia,the Americas,Europ...Introduction:Chikungunya virus(CHIKV),transmitted by Aedes aegypti and Aedes albopictus,causes chikungunya fever.Since its 2005 re-emergence,it has become endemic in 119 countries across Africa,Asia,the Americas,Europe,and Oceania.An outbreak in China’s Guangdong Province(July 2025)led to more than 15,000 cases by August 20,straining public health systems and highlighting the need to strengthen viral mutation surveillance.However,the precise genomic characteristics of this prevalent virus remain unknown,and this study aims to fill this critical knowledge gap through high-throughput sequencing technology.Methods:This study collected two serum samples from this epidemic,extracting and sequencing total nucleic acids via an MGISEQ-G99 sequencer.Complete viral genomes were generated via a consensus reference assembly;they were phylogenetically analyzed and comparatively assessed to identify viral nucleotide variations and protein amino acid substitutions.Results:Phylogenetic analysis confirmed that both research strains belong to the East/Central/South African genotype,clustering within the Middle African Lineage and sharing the highest identity with currently circulating CHIKV isolates from Réunion Island.The circulating strain carries adaptive mutation sites such as E1-A226V,E2-L210Q,and E2-I211T,significantly enhancing viral replication efficiency in Ae.albopictus.Conclusions:Understanding viral etiology is essential for controlling outbreaks.The current epidemic strain carries mutations adaptive to Ae.albopictus,increasing transmission risk.Guangdong’s mosquito population—predominantly Ae.albopictus with limited Ae.aegypti presence—facilitates efficient virus import and spread.展开更多
基金Supported by Shenzhen Medical Research Fund(B2404002)Sanming Project of Medicine in Shenzhen(SZSM202211023)Shenzhen Science and Technology Program(SYSPG20241211173921049,SGDX20240115113259001).
文摘Introduction:Chikungunya virus(CHIKV),transmitted by Aedes aegypti and Aedes albopictus,causes chikungunya fever.Since its 2005 re-emergence,it has become endemic in 119 countries across Africa,Asia,the Americas,Europe,and Oceania.An outbreak in China’s Guangdong Province(July 2025)led to more than 15,000 cases by August 20,straining public health systems and highlighting the need to strengthen viral mutation surveillance.However,the precise genomic characteristics of this prevalent virus remain unknown,and this study aims to fill this critical knowledge gap through high-throughput sequencing technology.Methods:This study collected two serum samples from this epidemic,extracting and sequencing total nucleic acids via an MGISEQ-G99 sequencer.Complete viral genomes were generated via a consensus reference assembly;they were phylogenetically analyzed and comparatively assessed to identify viral nucleotide variations and protein amino acid substitutions.Results:Phylogenetic analysis confirmed that both research strains belong to the East/Central/South African genotype,clustering within the Middle African Lineage and sharing the highest identity with currently circulating CHIKV isolates from Réunion Island.The circulating strain carries adaptive mutation sites such as E1-A226V,E2-L210Q,and E2-I211T,significantly enhancing viral replication efficiency in Ae.albopictus.Conclusions:Understanding viral etiology is essential for controlling outbreaks.The current epidemic strain carries mutations adaptive to Ae.albopictus,increasing transmission risk.Guangdong’s mosquito population—predominantly Ae.albopictus with limited Ae.aegypti presence—facilitates efficient virus import and spread.
