Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-c...Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-cropping system, 4 main plots （fertilization levels） and 6 subplots （planting densities） were set in a split plot design. The results suggested that yield had close relationship with fertilization levels and planting densities. Different fertilization levels and planting densities significantly affected yield. With the increase of nitrogen fertilization, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency declined. Under the same fertilization level, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency grew a little with the increase of planting density, so nitrogen efficiency could be improved by regulating planting density. The results also showed that A2 （including N 225.0 kg/hm2, P205 75.0 kg/hm^2, K20 187.5 kg/hm^2） matching to B3 （52 500 plants/hm^2） or B4（60 000 plants/hm^2） was a better design, which could obtain a higher yield in the range of 7 913.2-8 207.8 kg/hm2, respectively.展开更多
Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place...Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place as the spermatozoa pass through the female reproductive tract(FRT).Dihydrolipoamide dehydrogenase(DLD)protein is a post-pyruvate metabolic enzyme,exhibiting reactive oxygen species(ROS)production which causes capacitation.Additionally,other vital functions of DLD in buffalo spermatozoa are hyperactivation and acrosome reaction.DLD produces the optimum amount of ROS required to induce capacitation process in FRT.Depending on physiological or patho-physiological conditions,DLD can either enhance or attenuate the production of reactive oxygen species(ROS).Aim of this study was to investigate whether changes in the production of ROS in sperm cells can impact their ability to fertilize by triggering the capacitation and acrosome reaction.Results In this study,abundance of DLD protein was quantified between high(n=5)and low fertile bull(n=5)sper-matozoa.It was found that compared to high-fertile(HF)bulls,low-fertile(LF)bulls exhibited significantly(P<0.05)higher DLD abundances.Herein,we optimised the MICA concentration to inhibit DLD function,spermatozoa were treated with MICA in time(0,1,2,3,4,and 5 h)and concentrations(1,2.5,5,and 10 mmol/L)dependent manner.Maximum DLD inhibition was found to be at 4 h in 10 mmol/L MICA concentration,which was used for further exper-imentation in HF and LF.Based on DLD inhibition it was seen that LF bull spermatozoa exhibited significantly(P<0.05)higher ROS production and acrosome reaction in comparison to the HF bull spermatozoa.The kinematic parameters of the spermatozoa such as percent total motility,velocity parameters(VCL,VSL,and VAP)and other parameters(BCF,STR,and LIN)were also decreased in MICA treated spermatozoa in comparison to the control(capacitated)spermatozoa.Conclusions The present study provides an initial evidence explaining the buffalo bull spermatozoa with higher DLD abundance undergo early capacitation,which subsequently reduces their capacity to fertilize.展开更多
Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hain...Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hainan Province. The results showed that the optimal fertilizing amount of ternary quadratic model simulation was0.374 kg/plant of N, 0.289 kg/plant of P2O5 and 0.891 kg/plant of K2 O. According to the yield trend characteristic, the optimal fertilizing amount of unary quadratic model was 0.400kg/plant of N, 0.214 kg/plant of P2O5 and 0.901kg/plant of K2 O. Thus it can be seen that only partial indices of the optimal fertilizing amount of ternary quadratic model simulation were higher than that of unary quadratic model. Considering the results, the optimal fertilizing amount of Brazil banana was 0.374-0.400kg/plant of N, 0.214-0.289 kg/plant of P2O5 and 0.891-0.901 kg/plant of K2 O.展开更多
In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filmin...In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filming, fertilizing and sowing on one for foxtail millet was formed through the integration of plastic film mulching technology and mechanized production technology by Institute of Millet crops of Hebei Academy of Agriculture and Forestry Sciences, and the techniques were introduced from the key technologies of pre-sowing preparation, sowing, supporting equipment, field management, harvesting, plastic film recycling.展开更多
The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger ...The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger systems such as the cAMP/adenylate cyclase （AC） system and the cGMP/guanylate cyclase （GC） system appear to regulate sperm functions. Increased levels of intracytosolic cAMP result in an enhancement of sperm motility and viability. The stimulation of GC by low doses of nitric oxide （NO） leads to an improvement or maintenance of sperm motility, whereas higher concentrations have an adverse effect on sperm parameters. Several in vivo and in vitro studies have been carried out in order to examine whether PDE5 inhibitors affect positively or negatively sperm parameters and sperm fertilizing capacity. The results of these studies are controversial. Some of these studies demonstrate no significant effects of PDE5 inhibitors on the motility, viability, and morphology of spermatozoa collected from men that have been treated with PDE5 inhibitors. On the other hand, several studies demonstrate a positive effect of PDE5 inhibitors on sperm motility both in vivo and in vitro. In vitro studies of sildenafil citrate demonstrate a stimulatory effect on sperm motility with an increase in intracellular cAMP suggesting an inhibitory action of sildenafil citrate on a PDE isoform other than the PDE5. On the other hand, tadalafil＇s actions appear to be associated with the inhibitory effect of this compound on PDE11. In vivo studies in men treated with vardenafil in a daily basis demonstrated a significantly larger total number of spermatozoa per ejaculate, quantitative sperm motility, and qualitative sperm motility; it has been suggested that vardenafil administration enhances the secretory function of the prostate and subsequently increases the qualitative and quantitative motility of spermatozoa. The effect that PDE5 inhibitors exert on sperm parameters may lead to the improvement of the outcome of assisted reproductive technology （ART） programs. In the future PDE5 inhibitors might serve as adjunct therapeutical agents for the alleviation of male infertility.展开更多
The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Ferti...The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Fertilizer rates of K2O 135 and 270 kg/hm2, representing 1x and 2x recommended K rates, were applied, no application of k fertilizer as the CK. The results show that the lint yield increased 39.13%-57.48%with potassium application, highly significantly. Al yield components of the three hy-brid cotton varieties increased with the increase of K application amounts. The bol number per plant, single bol weight and lint percentage were increased by 14.24%-40.29%, 3.59%-15.51% and 0.16%-4.89%, respectively, and the fiber length and specific strength also increased with the increasing K application amounts, showing no significant influence on Micronaire. When the K application amounts increased from 135 to 270 kg/hm2, the partial factor productivity （PFPk） reduced by 45.93%-48.01%, and the agronomic efficiency （AEk） reduced by 37.1%-42.9%. The PFPk and KE （K efficiency coefficients） of S328 were the highest among the three varieties, which also showed the strongest resistance to low potassium stress, and with no potassi-um fertilizer application （K0）, the lint yield of S328 was 5.54% and 11.19% higher than that of X8 and J102. The AEk of J102 was the highest, and its reward of K fertilizer was the greatest among the three varieties.展开更多
The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in f...The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.展开更多
To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amo...To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amount and planting density on yield and quality of material.The results showed that Taishan 27 had high yield under fertilizing amount of 225 kg/ha pure nitrogen and planting density of 240 × 104-300× 10~4/ha; the yield was lowest under fertilizing amount of 300 kg/ha pure nitrogen and planting density of 360 × 10~4/ha. The suitable planting density for Taishan 27 was 240 × 104-300 × 10~4/ha,and the fertilizing amount of nitrogen should be based on different soil fertility conditions to avoid water and fertilizer stress and improve yield.展开更多
Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gam...Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gametogenesis. Less is known about the effects cholesterol homeostasis may have on postgonadic reproductive functions. Lately, several data have been reported showing how imbalanced cholesterol levels may particularly affect the post-testicular events of sperm maturation that lead to fully fertile male gametes. This review will focus on that aspect and essentially centers on how cholesterol is important for the physiology of the mammalian epididymis and spermatozoa.展开更多
Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of...Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances （EPS） from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To investigate the impact of EPS on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal status, hypo-osmotic swelling, and in vitro fertilization rate after incubating the sperm with EPS in vitro using mice. The incubation of sperm with EPS significantly decreased sperm motility, viability, and functional integrity in a concentration and time-dependent manner. These effects on sperm quality also resulted in a decreased fertilization rate in vitro. This is the first report that demonstrates the direct negative impact of the EPS of T. vaginalis on the fertilization rate of sperm in vitro. However, further study should be performed using human sperm to determine if EPS has similar negative impact on human sperm fertilizing capacity in vitro.展开更多
The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while sta...The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while stalk makes less contribution. The most optimal pattern of fertilizing materials is 28 t / ha of farm manure, 630 kg / ha chemical fertilizers and 2.41 / ha of stalk.展开更多
Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of s...Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood.The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in cattle.Results:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and calcium.Insemination of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P<0.01),whereas the rate of sperm with moderate+high single-stranded breaks was higher in the second period(0-2 h:3.52±7.77%/h vs.2-4h:21.06±11.69%/h;P<0.0001).Regarding sperm physiology,viability decrease rate was different between the two periods(0-2 h:-4.49±1.79%/h vs.2-4 h:-2.50±3.39%/h;P=0.032),but the progressive motility decrease rate was constant throughout post-thawing incubation(0-2 h:-4.70±3.42%/h vs.2-4 h:-1.89±2.97%/h;P>0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage and chromatin deprotamination suggested a sequence of events all driven by oxidative stress,where viability and motility would be affected first and sperm chromatin would be altered at a later stage,thus suggesting that bovine sperm should be used for fertilization within 2 h post-thaw.Fertility correlations supported that the assessment of global DNA damage through the Comet assay may help predict bull fertility.展开更多
This research study explored the efficacy of leaf litter compost as a sustainable soil amendment with the objective of promoting soil health and mitigating the accumulation of potentially toxic elements. The investiga...This research study explored the efficacy of leaf litter compost as a sustainable soil amendment with the objective of promoting soil health and mitigating the accumulation of potentially toxic elements. The investigation encompassed the impact of various organic compost amendments, including leaf compost, cow dung manure, kitchen waste compost, municipal organic waste compost, and vermicompost. The study employed Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to evaluate soil nutrient levels and concentrations of Potentially Toxic Elements (PTEs) such as arsenic, chromium, cadmium, mercury, lead, nickel, and lithium. The fertilization and bioremediation potential of these compost amendments are quantified using an indexing method. Results indicated a substantial increase in overall nutrient levels (carbon, nitrogen, phosphorus, potassium, and sulfur) in soils treated with leaf compost and other organic composts. Fertility indices (FI) are notably higher in compost-amended soils (ranging from 2.667 to 3.938) compared to those amended with chemical fertilizers (ranging from 2.250 to 2.813) across all soil samples. Furthermore, the mean concentrations of PTEs were significantly lower in soils treated with leaf compost and other organic compost amendments compared to those treated with chemical fertilizers amendments. The assessment through the indexing method revealed a high clean index (CI) for leaf compost amendment (ranging from 3.407 to 3.58), whereas the chemical fertilizer amendment exhibits a relatively lower CI (ranging from 2.78 to 3.20). Consequently, leaf compost and other organic composts exhibit the potential to enhance sustainable productivity, promoting soil health and environmental safety by improving nutrient levels and remediating potentially toxic elements in the soil.展开更多
A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK...A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK) and biological fertilizer (Azotobacter chroococcum, Bacillus megatherium, and Bacillus circulant) as recommended dose under new sandy soils conditions. Split plot designed with four treatments (Control, (50% Mineral fertilizer (M.) + 50% Biological fertilizer (Bio.)), 100% M. and 100% Bio.) of each species. Vegetative growth, leaf area, tree biomass, stored carbon, basal area, tree volume, and in the soil both of microbial account and mineral content were determined. The experimental results showed no significant differences between studied species among the most studied parameters except for Khaya senegalensis which gave the highest significant difference in root biomass and below-stored carbon than Swietenia mahagoni. Evidently, the highest significant growth parameters were 100% mineral fertilizer followed by (50% M. + 50% Bio.) as compared with control. No significant difference between 100% M. and (50% M. + 50% Bio.) of shoot dry biomass (15.19 and 12.02 kg, respectively) and above-stored carbon (0.28 and 0.22 Mt, respectively). Microbial account and mineral content in soil were improved after cultivation of tree species compared to before planting and control, especially with 50% mineral fertilizer and 50% bio-fertilizer treatment. In conclusion, a treatment containing 50% mineral fertilizer and 50% bio-fertilizer has led to the ideal Khaya senegalensis and Swietenia mahagoni growth in sandy soil for cheaper and sustainable.展开更多
Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassi...Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.展开更多
The continuous supply of phosphorus(P)is indispensable in crop production.However,P resources are non-renewable,and environmental concerns like eutrophication associated with its loss from agroecosystems make the sust...The continuous supply of phosphorus(P)is indispensable in crop production.However,P resources are non-renewable,and environmental concerns like eutrophication associated with its loss from agroecosystems make the sustainable management of P resources essential for ensuring global food security.This study was designed to reduce mineral P inputs through management practices.A field experiment comprising a wheat-maize rotation system was conducted in the Guanzhong Plain of Shaanxi Province,China from 2018-2023.The eight treatments included CK(without P),FP(conventional P application);RP(recommended P);RP80(20% reduction in RP);SRP80(20% reduction in RP with straw wrapping);ARP80(20% reduction in RP with ammonium sulfate instead of urea);SARP80(20% reduction in RP with straw wrapping and ammonium sulfate instead of urea);and SARP60(40% reduction in RP with straw wrapping and ammonium sulfate instead of urea).Crop yield,P uptake,and P fertilizer use efficiency were measured during harvest and throughout the entire period of the study.At the end of the experiment,P fractions were estimated using the Tiessen-Moir P classification method.The results revealed that the grain yields of all the treatments except for RP80 were significantly increased compared to CK,with increases of 14.9-28.8%.Furthermore,agronomic efficiency,apparent P use efficiency,P recovery rate,and partial factor productivity were significantly improved for the treatments that received 20% less P with straw wrapping.Moreover,the enhancement measures significantly increased labile and moderately labile P in the soil.Therefore,straw wrapping with ammonium sulfate instead of urea is one of the most effective ways to reduce mineral P inputs while increasing the efficiency of P in wheat-maize rotation systems.展开更多
Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and ...Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and the environment.Estimating ammonia emissions is crucial for national inventories and policy-making.Various models exist for predicting emissions,including mechanistic,empirical,and semi-empirical approaches.While machine learning(ML)is widely used in environmental science,its application to ammonia emissions remains limited.In this study,we used 5939 ammonia emission data from 538 trials,extracted from the ALFAM2 database,to train three machine learning methods-random forest,gradient boosting,and lasso-for predicting cumulative ammonia emissions 72 h after manure application.These methods were compared to the semi-empirical ALFAM2 model using an independent test dataset.Random forest(RMSE=4.51,r=0.94,MAE=3.28,Bias=0.92)and gradient boosting(RMSE=6.19,r=0.89,MAE=4.10,Bias=0.51)showed the best performance,while the lasso log-linear model(RMSE=7.30,r=0.84,MAE=5.57,Bias=-1.38)performed worst.Both random forest and gradient boosting outperformed the semi-empirical ALFAM2 model,which showed performance comparable to the lasso model.We then used these models and the ALFAM2 model to compare five slurry management techniques,varying in application method(trailing hoses,trailing shoes,and open slot)and post-application incorporation,across 128 scenarios with different manure types and weather conditions.Compared to broadcast application,alternative techniques reduced emissions by a median of-13.6%to-61.7%.This study highlights the promise of ML models in assessing ammonia emission reduction methods,while emphasizing the importance of evaluating model sensitivity to algorithm choice.展开更多
Background Understanding the genetic basis of male reproduction in mammals remains challenging.Commercial pig populations offer a unique model for studying fertility,as semen traits are routinely recorded using high-t...Background Understanding the genetic basis of male reproduction in mammals remains challenging.Commercial pig populations offer a unique model for studying fertility,as semen traits are routinely recorded using high-throughput systems.Results In a large-scale GWAS of 15 semen traits based on 286,314 ejaculates collected from 2,954 boars of a purebred pig line,we identified 10 QTL,including four loci with recessive deleterious alleles.Several lead SNPs affected multiple semen traits.For example,a SNP on SSC6 was significantly associated with distal cytoplasmic droplets and with effects on tail abnormalities and sperm motility in a follow up analysis.The allele frequencies of some loci were different in older boar's,most likely due to culling based on poor semen quality.Using WGS,we identified six missense variants in high linkage disequilibrium(LD)with lead SNPs in genes related to sperm production(e.g.,MEIOB,CFAP74 and UBE2B).Remarkably,the frequency of some alleles with predicted deleterious effects on semen traits increased between 2013 and 2019.Conclusions Our results highlight loci with major effects on semen quality,some of which are linked to functional variants in key genes involved in spermatogenesis.The information from this study can be used to select against deleterious alleles affecting semen characteristics in pigs and provides valuable insight into the genetics of mammalian male fertility.展开更多
Soil fertility and forest structure influence tree carbon stocks.However,it remains unclear how tree mycorrhizal types affect these relationships.This study addressed the question of how aboveground and belowground tr...Soil fertility and forest structure influence tree carbon stocks.However,it remains unclear how tree mycorrhizal types affect these relationships.This study addressed the question of how aboveground and belowground tree carbon stocks in soils with different mycorrhizal types are affected by soil fertility and forest structure.Tree demographic data were used from a 21.12-ha study area collected over a ten-year period(2009-2019),covering 43species of woody plants and more than 50,000 individuals.Relationships between tree carbon stock,soil fertility and forest structure(stand density,diameter variation,species diversity and spatial distribution)were examined,as well as whether these relationships differed between arbuscular mycorrhiza and ectomycorrhizal mycorrhiza groups in a typical temperate conifer and broad-leaved mixed forest.We found that total tree carbon stock was positively impacted by variations in stand density and tree diameter but negatively influenced by soil fertility,tree species diversity and uniform angle index.Soil fertility promoted carbon stock of trees associated with arbuscular mycorrhiza(AM)but inhibited the carbon stock of trees with ectomycorrhizal mycorrhiza fungi(EcM).Carbon stock of AM trees was mainly influenced by soil fertility,while carbon stock of EcM trees was influenced by stand density.Our findings show that mycorrhizae types mediate the impact of stand structure and soil fertility on tree carbon stocks and provides new evidence on how forest tree carbon stocks may be enhanced based on the types of mycorrhizal associations.Tree species with different mycorrhizal types can be managed in different ways.展开更多
Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,...Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,titanium,iodine,vanadium,cobalt,sodium,and rare earth elements.They are not essential for all plants,but some are crucial for specific plant species.However,the mechanisms of action of many beneficial elements are still unclear,and products containing beneficial elements have not been widely accepted and used by the public.This review systematically summarizes the current knowledge of plant-beneficial elements.Most importantly,we offer suggestions for future research on beneficial elements,which include integrating cross-disciplinary and innovative technologies,expanding the scope of application and elemental spies,broadening the spatial and temporal scales of research,incorporating beneficial elements into the soil health evaluation system,and shifting from single to multi-element applications.In the future,research on beneficial elements should be closely centered around“mechanism+application”to meet the ever-increasing demands driven by population growth,improve human health,tackle environmental challenges,and promote rural economic development.展开更多
文摘Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-cropping system, 4 main plots （fertilization levels） and 6 subplots （planting densities） were set in a split plot design. The results suggested that yield had close relationship with fertilization levels and planting densities. Different fertilization levels and planting densities significantly affected yield. With the increase of nitrogen fertilization, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency declined. Under the same fertilization level, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency grew a little with the increase of planting density, so nitrogen efficiency could be improved by regulating planting density. The results also showed that A2 （including N 225.0 kg/hm2, P205 75.0 kg/hm^2, K20 187.5 kg/hm^2） matching to B3 （52 500 plants/hm^2） or B4（60 000 plants/hm^2） was a better design, which could obtain a higher yield in the range of 7 913.2-8 207.8 kg/hm2, respectively.
基金Bill&Melinda Gates Foundation(Grant number OPP1154401).
文摘Backgroud Before fertilization,spermatozoa undergo a crucial maturation step called capacitation,which is a unique event regulates the sperm’s ability for successful fertilization.The capacitation process takes place as the spermatozoa pass through the female reproductive tract(FRT).Dihydrolipoamide dehydrogenase(DLD)protein is a post-pyruvate metabolic enzyme,exhibiting reactive oxygen species(ROS)production which causes capacitation.Additionally,other vital functions of DLD in buffalo spermatozoa are hyperactivation and acrosome reaction.DLD produces the optimum amount of ROS required to induce capacitation process in FRT.Depending on physiological or patho-physiological conditions,DLD can either enhance or attenuate the production of reactive oxygen species(ROS).Aim of this study was to investigate whether changes in the production of ROS in sperm cells can impact their ability to fertilize by triggering the capacitation and acrosome reaction.Results In this study,abundance of DLD protein was quantified between high(n=5)and low fertile bull(n=5)sper-matozoa.It was found that compared to high-fertile(HF)bulls,low-fertile(LF)bulls exhibited significantly(P<0.05)higher DLD abundances.Herein,we optimised the MICA concentration to inhibit DLD function,spermatozoa were treated with MICA in time(0,1,2,3,4,and 5 h)and concentrations(1,2.5,5,and 10 mmol/L)dependent manner.Maximum DLD inhibition was found to be at 4 h in 10 mmol/L MICA concentration,which was used for further exper-imentation in HF and LF.Based on DLD inhibition it was seen that LF bull spermatozoa exhibited significantly(P<0.05)higher ROS production and acrosome reaction in comparison to the HF bull spermatozoa.The kinematic parameters of the spermatozoa such as percent total motility,velocity parameters(VCL,VSL,and VAP)and other parameters(BCF,STR,and LIN)were also decreased in MICA treated spermatozoa in comparison to the control(capacitated)spermatozoa.Conclusions The present study provides an initial evidence explaining the buffalo bull spermatozoa with higher DLD abundance undergo early capacitation,which subsequently reduces their capacity to fertilize.
基金Supported by the National Science Found for Young Scholars of China(No.31101123)Natural Science Foundation of Hainan Province of China(No.311062)~~
文摘Fertilizer effect model such as ternary quadratic, unary quadratic, straight line and platform model was respectively used to analyze the two-year ＂3414＂ test data collected from banana garden in Fushan Town of Hainan Province. The results showed that the optimal fertilizing amount of ternary quadratic model simulation was0.374 kg/plant of N, 0.289 kg/plant of P2O5 and 0.891 kg/plant of K2 O. According to the yield trend characteristic, the optimal fertilizing amount of unary quadratic model was 0.400kg/plant of N, 0.214 kg/plant of P2O5 and 0.901kg/plant of K2 O. Thus it can be seen that only partial indices of the optimal fertilizing amount of ternary quadratic model simulation were higher than that of unary quadratic model. Considering the results, the optimal fertilizing amount of Brazil banana was 0.374-0.400kg/plant of N, 0.214-0.289 kg/plant of P2O5 and 0.891-0.901 kg/plant of K2 O.
基金Supported by the National Key Technology R&D Program(2014BAD07B01-02)the Science and Technology Demonstration Project of Bohai Granary in Hebei Provincethe Special Fund for Agro-scientific Research in Public Interest(201303133-1-6)~~
文摘In view of the problems of completely depending on rain, low and unstable yield and complicated planting of dry land foxtail millet, the light simplified cultivation techniques of wide row and double ridge with filming, fertilizing and sowing on one for foxtail millet was formed through the integration of plastic film mulching technology and mechanized production technology by Institute of Millet crops of Hebei Academy of Agriculture and Forestry Sciences, and the techniques were introduced from the key technologies of pre-sowing preparation, sowing, supporting equipment, field management, harvesting, plastic film recycling.
文摘The aim of this review study is to elucidate the effects that phosphodiesterase 5 （PDE5） inhibitors exert on spermatozoa motility, capacitation process and on their ability to fertilize the oocyte. Second messenger systems such as the cAMP/adenylate cyclase （AC） system and the cGMP/guanylate cyclase （GC） system appear to regulate sperm functions. Increased levels of intracytosolic cAMP result in an enhancement of sperm motility and viability. The stimulation of GC by low doses of nitric oxide （NO） leads to an improvement or maintenance of sperm motility, whereas higher concentrations have an adverse effect on sperm parameters. Several in vivo and in vitro studies have been carried out in order to examine whether PDE5 inhibitors affect positively or negatively sperm parameters and sperm fertilizing capacity. The results of these studies are controversial. Some of these studies demonstrate no significant effects of PDE5 inhibitors on the motility, viability, and morphology of spermatozoa collected from men that have been treated with PDE5 inhibitors. On the other hand, several studies demonstrate a positive effect of PDE5 inhibitors on sperm motility both in vivo and in vitro. In vitro studies of sildenafil citrate demonstrate a stimulatory effect on sperm motility with an increase in intracellular cAMP suggesting an inhibitory action of sildenafil citrate on a PDE isoform other than the PDE5. On the other hand, tadalafil＇s actions appear to be associated with the inhibitory effect of this compound on PDE11. In vivo studies in men treated with vardenafil in a daily basis demonstrated a significantly larger total number of spermatozoa per ejaculate, quantitative sperm motility, and qualitative sperm motility; it has been suggested that vardenafil administration enhances the secretory function of the prostate and subsequently increases the qualitative and quantitative motility of spermatozoa. The effect that PDE5 inhibitors exert on sperm parameters may lead to the improvement of the outcome of assisted reproductive technology （ART） programs. In the future PDE5 inhibitors might serve as adjunct therapeutical agents for the alleviation of male infertility.
基金Supported by the Project for Cotton Industry of Hunan Province(Xiangnongyelian[2012]No.278)the Foundation for Talents Stabilization of Hunan Agricultural University(09WD19)~~
文摘The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Fertilizer rates of K2O 135 and 270 kg/hm2, representing 1x and 2x recommended K rates, were applied, no application of k fertilizer as the CK. The results show that the lint yield increased 39.13%-57.48%with potassium application, highly significantly. Al yield components of the three hy-brid cotton varieties increased with the increase of K application amounts. The bol number per plant, single bol weight and lint percentage were increased by 14.24%-40.29%, 3.59%-15.51% and 0.16%-4.89%, respectively, and the fiber length and specific strength also increased with the increasing K application amounts, showing no significant influence on Micronaire. When the K application amounts increased from 135 to 270 kg/hm2, the partial factor productivity （PFPk） reduced by 45.93%-48.01%, and the agronomic efficiency （AEk） reduced by 37.1%-42.9%. The PFPk and KE （K efficiency coefficients） of S328 were the highest among the three varieties, which also showed the strongest resistance to low potassium stress, and with no potassi-um fertilizer application （K0）, the lint yield of S328 was 5.54% and 11.19% higher than that of X8 and J102. The AEk of J102 was the highest, and its reward of K fertilizer was the greatest among the three varieties.
文摘The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.
基金Supported by National Modern Agricultural Technology System for Wheat(CARS-3-2-22)Special Research Fund for National Public Service Sector(Agriculture)(201203033)+1 种基金Modern Agricultural Industry Technology System Project(nycytx-03)Shandong Modern Agricultural Industry Technology System(SDAIT-04-021-12)
文摘To get the cultivation pattern featured by improved varieties and fine methods for strong-gluten and high-yielding wheat variety Taishan 27,this paper used Taishan 27 as material to study the effect of fertilizing amount and planting density on yield and quality of material.The results showed that Taishan 27 had high yield under fertilizing amount of 225 kg/ha pure nitrogen and planting density of 240 × 104-300× 10~4/ha; the yield was lowest under fertilizing amount of 300 kg/ha pure nitrogen and planting density of 360 × 10~4/ha. The suitable planting density for Taishan 27 was 240 × 104-300 × 10~4/ha,and the fertilizing amount of nitrogen should be based on different soil fertility conditions to avoid water and fertilizer stress and improve yield.
文摘Cholesterol, being the starting point of steroid hormone synthesis, is a long known modulator of both female and male reproductive physiology especially at the level of the gonads and the impact cholesterol has on gametogenesis. Less is known about the effects cholesterol homeostasis may have on postgonadic reproductive functions. Lately, several data have been reported showing how imbalanced cholesterol levels may particularly affect the post-testicular events of sperm maturation that lead to fully fertile male gametes. This review will focus on that aspect and essentially centers on how cholesterol is important for the physiology of the mammalian epididymis and spermatozoa.
文摘Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances （EPS） from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To investigate the impact of EPS on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal status, hypo-osmotic swelling, and in vitro fertilization rate after incubating the sperm with EPS in vitro using mice. The incubation of sperm with EPS significantly decreased sperm motility, viability, and functional integrity in a concentration and time-dependent manner. These effects on sperm quality also resulted in a decreased fertilization rate in vitro. This is the first report that demonstrates the direct negative impact of the EPS of T. vaginalis on the fertilization rate of sperm in vitro. However, further study should be performed using human sperm to determine if EPS has similar negative impact on human sperm fertilizing capacity in vitro.
文摘The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while stalk makes less contribution. The most optimal pattern of fertilizing materials is 28 t / ha of farm manure, 630 kg / ha chemical fertilizers and 2.41 / ha of stalk.
基金the European Union’s Horizon 2020 Research and Innovation scheme under the Marie Sklodowska-Curie grant agreement No.801342(Tecniospring INDUSTRY,TECSPR-19-1-0003)the Ministry of Science and Innovation,Spain(AGL2017-88329-R and PID2020-113320RBI00)+2 种基金the Catalan Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(2017-SGR-1229)the Catalan Institution for Research and Advanced Studies(ICREA)La Maratóde TV3 Foundation(214/857-202039)。
文摘Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood.The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in cattle.Results:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and calcium.Insemination of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P<0.01),whereas the rate of sperm with moderate+high single-stranded breaks was higher in the second period(0-2 h:3.52±7.77%/h vs.2-4h:21.06±11.69%/h;P<0.0001).Regarding sperm physiology,viability decrease rate was different between the two periods(0-2 h:-4.49±1.79%/h vs.2-4 h:-2.50±3.39%/h;P=0.032),but the progressive motility decrease rate was constant throughout post-thawing incubation(0-2 h:-4.70±3.42%/h vs.2-4 h:-1.89±2.97%/h;P>0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage and chromatin deprotamination suggested a sequence of events all driven by oxidative stress,where viability and motility would be affected first and sperm chromatin would be altered at a later stage,thus suggesting that bovine sperm should be used for fertilization within 2 h post-thaw.Fertility correlations supported that the assessment of global DNA damage through the Comet assay may help predict bull fertility.
文摘This research study explored the efficacy of leaf litter compost as a sustainable soil amendment with the objective of promoting soil health and mitigating the accumulation of potentially toxic elements. The investigation encompassed the impact of various organic compost amendments, including leaf compost, cow dung manure, kitchen waste compost, municipal organic waste compost, and vermicompost. The study employed Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to evaluate soil nutrient levels and concentrations of Potentially Toxic Elements (PTEs) such as arsenic, chromium, cadmium, mercury, lead, nickel, and lithium. The fertilization and bioremediation potential of these compost amendments are quantified using an indexing method. Results indicated a substantial increase in overall nutrient levels (carbon, nitrogen, phosphorus, potassium, and sulfur) in soils treated with leaf compost and other organic composts. Fertility indices (FI) are notably higher in compost-amended soils (ranging from 2.667 to 3.938) compared to those amended with chemical fertilizers (ranging from 2.250 to 2.813) across all soil samples. Furthermore, the mean concentrations of PTEs were significantly lower in soils treated with leaf compost and other organic compost amendments compared to those treated with chemical fertilizers amendments. The assessment through the indexing method revealed a high clean index (CI) for leaf compost amendment (ranging from 3.407 to 3.58), whereas the chemical fertilizer amendment exhibits a relatively lower CI (ranging from 2.78 to 3.20). Consequently, leaf compost and other organic composts exhibit the potential to enhance sustainable productivity, promoting soil health and environmental safety by improving nutrient levels and remediating potentially toxic elements in the soil.
文摘A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK) and biological fertilizer (Azotobacter chroococcum, Bacillus megatherium, and Bacillus circulant) as recommended dose under new sandy soils conditions. Split plot designed with four treatments (Control, (50% Mineral fertilizer (M.) + 50% Biological fertilizer (Bio.)), 100% M. and 100% Bio.) of each species. Vegetative growth, leaf area, tree biomass, stored carbon, basal area, tree volume, and in the soil both of microbial account and mineral content were determined. The experimental results showed no significant differences between studied species among the most studied parameters except for Khaya senegalensis which gave the highest significant difference in root biomass and below-stored carbon than Swietenia mahagoni. Evidently, the highest significant growth parameters were 100% mineral fertilizer followed by (50% M. + 50% Bio.) as compared with control. No significant difference between 100% M. and (50% M. + 50% Bio.) of shoot dry biomass (15.19 and 12.02 kg, respectively) and above-stored carbon (0.28 and 0.22 Mt, respectively). Microbial account and mineral content in soil were improved after cultivation of tree species compared to before planting and control, especially with 50% mineral fertilizer and 50% bio-fertilizer treatment. In conclusion, a treatment containing 50% mineral fertilizer and 50% bio-fertilizer has led to the ideal Khaya senegalensis and Swietenia mahagoni growth in sandy soil for cheaper and sustainable.
基金funded by the National Natural Science Foundation of China (31760363)the Earmarked Fund for CARS (CARS-14-1-16)+1 种基金the Gansu Education Science and Technology Innovation Industry Support Program,China (2021CYZC-38)the Gansu Provincial Key Laboratory of Arid Land Crop Science,Gansu Agricultural University,China (GSCS-2020-Z6)。
文摘Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.
基金supported by the National Key Research and Development Program of China(2023YFD1900300 and 2017YFD0200205)the Agricultural Key-scientific and Core-technological Project of Shaanxi Province,China(2024NYGG011)。
文摘The continuous supply of phosphorus(P)is indispensable in crop production.However,P resources are non-renewable,and environmental concerns like eutrophication associated with its loss from agroecosystems make the sustainable management of P resources essential for ensuring global food security.This study was designed to reduce mineral P inputs through management practices.A field experiment comprising a wheat-maize rotation system was conducted in the Guanzhong Plain of Shaanxi Province,China from 2018-2023.The eight treatments included CK(without P),FP(conventional P application);RP(recommended P);RP80(20% reduction in RP);SRP80(20% reduction in RP with straw wrapping);ARP80(20% reduction in RP with ammonium sulfate instead of urea);SARP80(20% reduction in RP with straw wrapping and ammonium sulfate instead of urea);and SARP60(40% reduction in RP with straw wrapping and ammonium sulfate instead of urea).Crop yield,P uptake,and P fertilizer use efficiency were measured during harvest and throughout the entire period of the study.At the end of the experiment,P fractions were estimated using the Tiessen-Moir P classification method.The results revealed that the grain yields of all the treatments except for RP80 were significantly increased compared to CK,with increases of 14.9-28.8%.Furthermore,agronomic efficiency,apparent P use efficiency,P recovery rate,and partial factor productivity were significantly improved for the treatments that received 20% less P with straw wrapping.Moreover,the enhancement measures significantly increased labile and moderately labile P in the soil.Therefore,straw wrapping with ammonium sulfate instead of urea is one of the most effective ways to reduce mineral P inputs while increasing the efficiency of P in wheat-maize rotation systems.
基金the French state aid managed by the ANR under the“Investissements d’avenir”programme with the reference ANR-16-CONV-0003from the AgroEcoSystem department of INRAE.We are grateful to the INRAE MIGALE bioinformatics facility(MIGALE,INRAE,2020.Migale bioinformatics Facility,doi:10.15454/1.5572390655343293E12)for providing help and/or computing and/or storage resources.We are also grateful to Sasha Hafner for his help in reproducing some of the results of Hafner et al.(2019).
文摘Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and the environment.Estimating ammonia emissions is crucial for national inventories and policy-making.Various models exist for predicting emissions,including mechanistic,empirical,and semi-empirical approaches.While machine learning(ML)is widely used in environmental science,its application to ammonia emissions remains limited.In this study,we used 5939 ammonia emission data from 538 trials,extracted from the ALFAM2 database,to train three machine learning methods-random forest,gradient boosting,and lasso-for predicting cumulative ammonia emissions 72 h after manure application.These methods were compared to the semi-empirical ALFAM2 model using an independent test dataset.Random forest(RMSE=4.51,r=0.94,MAE=3.28,Bias=0.92)and gradient boosting(RMSE=6.19,r=0.89,MAE=4.10,Bias=0.51)showed the best performance,while the lasso log-linear model(RMSE=7.30,r=0.84,MAE=5.57,Bias=-1.38)performed worst.Both random forest and gradient boosting outperformed the semi-empirical ALFAM2 model,which showed performance comparable to the lasso model.We then used these models and the ALFAM2 model to compare five slurry management techniques,varying in application method(trailing hoses,trailing shoes,and open slot)and post-application incorporation,across 128 scenarios with different manure types and weather conditions.Compared to broadcast application,alternative techniques reduced emissions by a median of-13.6%to-61.7%.This study highlights the promise of ML models in assessing ammonia emission reduction methods,while emphasizing the importance of evaluating model sensitivity to algorithm choice.
基金the GEroNIMO(Genome and Epigenome Enabled Breeding in Monogastics)project.The GEroNIMO project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 101000236。
文摘Background Understanding the genetic basis of male reproduction in mammals remains challenging.Commercial pig populations offer a unique model for studying fertility,as semen traits are routinely recorded using high-throughput systems.Results In a large-scale GWAS of 15 semen traits based on 286,314 ejaculates collected from 2,954 boars of a purebred pig line,we identified 10 QTL,including four loci with recessive deleterious alleles.Several lead SNPs affected multiple semen traits.For example,a SNP on SSC6 was significantly associated with distal cytoplasmic droplets and with effects on tail abnormalities and sperm motility in a follow up analysis.The allele frequencies of some loci were different in older boar's,most likely due to culling based on poor semen quality.Using WGS,we identified six missense variants in high linkage disequilibrium(LD)with lead SNPs in genes related to sperm production(e.g.,MEIOB,CFAP74 and UBE2B).Remarkably,the frequency of some alleles with predicted deleterious effects on semen traits increased between 2013 and 2019.Conclusions Our results highlight loci with major effects on semen quality,some of which are linked to functional variants in key genes involved in spermatogenesis.The information from this study can be used to select against deleterious alleles affecting semen characteristics in pigs and provides valuable insight into the genetics of mammalian male fertility.
基金supported by the Science and Technology Project of the Department of Transportation of Heilongjiang Province(HJK2023B024-3)the National Key R&D Program of China(2023YFF1304001-01)。
文摘Soil fertility and forest structure influence tree carbon stocks.However,it remains unclear how tree mycorrhizal types affect these relationships.This study addressed the question of how aboveground and belowground tree carbon stocks in soils with different mycorrhizal types are affected by soil fertility and forest structure.Tree demographic data were used from a 21.12-ha study area collected over a ten-year period(2009-2019),covering 43species of woody plants and more than 50,000 individuals.Relationships between tree carbon stock,soil fertility and forest structure(stand density,diameter variation,species diversity and spatial distribution)were examined,as well as whether these relationships differed between arbuscular mycorrhiza and ectomycorrhizal mycorrhiza groups in a typical temperate conifer and broad-leaved mixed forest.We found that total tree carbon stock was positively impacted by variations in stand density and tree diameter but negatively influenced by soil fertility,tree species diversity and uniform angle index.Soil fertility promoted carbon stock of trees associated with arbuscular mycorrhiza(AM)but inhibited the carbon stock of trees with ectomycorrhizal mycorrhiza fungi(EcM).Carbon stock of AM trees was mainly influenced by soil fertility,while carbon stock of EcM trees was influenced by stand density.Our findings show that mycorrhizae types mediate the impact of stand structure and soil fertility on tree carbon stocks and provides new evidence on how forest tree carbon stocks may be enhanced based on the types of mycorrhizal associations.Tree species with different mycorrhizal types can be managed in different ways.
基金jointly supported by grants from the National Natural Science Foundation of China(324B2064 and 32272799)the Fundamental Research Funds for the Central Universities,China(226-2024-00052)。
文摘Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,titanium,iodine,vanadium,cobalt,sodium,and rare earth elements.They are not essential for all plants,but some are crucial for specific plant species.However,the mechanisms of action of many beneficial elements are still unclear,and products containing beneficial elements have not been widely accepted and used by the public.This review systematically summarizes the current knowledge of plant-beneficial elements.Most importantly,we offer suggestions for future research on beneficial elements,which include integrating cross-disciplinary and innovative technologies,expanding the scope of application and elemental spies,broadening the spatial and temporal scales of research,incorporating beneficial elements into the soil health evaluation system,and shifting from single to multi-element applications.In the future,research on beneficial elements should be closely centered around“mechanism+application”to meet the ever-increasing demands driven by population growth,improve human health,tackle environmental challenges,and promote rural economic development.
