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Optimized nitrogen and potassium fertilizers application increases stem lodging resistance and grain yield of oil flax by enhancing lignin biosynthesis
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作者 Xiqiang Li Yuhong Gao +8 位作者 Zhengjun Cui Tingfeng Zhang Shiyuan Chen Shilei Xiang Lingling Jia Bin Yan Yifan Wang Lizhuo Guo Bing Wu 《Journal of Integrative Agriculture》 2026年第2期659-670,共12页
Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassi... Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions. 展开更多
关键词 oil flax nitrogen fertilizer potassium fertilizer stem lodging resistance lignin biosynthesis YIELD
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宫颈癌组织中M-CSF、bFGF、EPO表达水平与病理特征及预后关系
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作者 赵玉玲 崔海春 +1 位作者 李大海 吴欣欣 《北华大学学报(自然科学版)》 2026年第1期66-71,共6页
目的探讨宫颈癌组织中巨噬细胞集落刺激因子(M-CSF)、碱性成纤维细胞生长因子(bFGF)、促红细胞生成素(EPO)表达水平与病理特征及预后的关系。方法选取宫颈癌患者70例(宫颈癌组)、宫颈上皮内瘤变患者35例(宫颈上皮内瘤变组)、宫颈炎患者3... 目的探讨宫颈癌组织中巨噬细胞集落刺激因子(M-CSF)、碱性成纤维细胞生长因子(bFGF)、促红细胞生成素(EPO)表达水平与病理特征及预后的关系。方法选取宫颈癌患者70例(宫颈癌组)、宫颈上皮内瘤变患者35例(宫颈上皮内瘤变组)、宫颈炎患者35例(对照组)。在行阴道镜检查时,分别采集宫颈癌组织标本、宫颈上皮内瘤变组织标本、正常宫颈组织标本,应用免疫组化法检测M-CSF、bFGF、EPO表达水平。收集宫颈癌患者的临床病理参数并随访18个月评估预后。结果宫颈癌组患者M-CSF、bFGF、EPO表达阳性率高于宫颈上皮内瘤变组和对照组(P<0.01)。FIGO分期Ⅱ期、有淋巴结转移、低分化的宫颈癌患者M-CSF、bFGF、EPO表达水平明显升高,且M-CSF表达与bFGF、EPO表达水平呈正相关关系(P<0.01)。随访期间,复发的宫颈癌患者M-CSF、bFGF、EPO表达水平高于未复发的患者(P<0.05)。多因素分析显示,FIGO分期、分化程度及M-CSF、bFGF、EPO表达水平是患者预后的独立影响因素。基于上述指标构建的列线图模型C-index为0.927,表明该预测模型具有良好的区分度。结论宫颈癌组织中M-CSF、bFGF、EPO高表达且互相关联,结合FIGO分期、分化程度及M-CSF、bFGF、EPO表达水平构建的列线图预测模型可有效预测患者预后。 展开更多
关键词 宫颈癌 巨噬细胞集落刺激因子 碱性成纤维细胞生长因子 促红细胞生成素 预后
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GM-CSF和IL-6的免疫佐剂活性及对小鼠生长性能、血液指标的影响
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作者 成伟伟 容维中 +5 位作者 杨明 李元新 赵子惠 陈伯祥 王佳 周瑶 《饲料工业》 北大核心 2025年第1期95-102,共8页
为验证粒细胞-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素6(IL-6)的免疫佐剂活性以及研究其对小鼠生长性能、血液指标的影响,试验扩增了GM-CSF基因和IL-6基因,并将GMCSF基因和IL-6基因分别克隆至载体p CDNA3.1-His-C和p ET-32a(+),且诱... 为验证粒细胞-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素6(IL-6)的免疫佐剂活性以及研究其对小鼠生长性能、血液指标的影响,试验扩增了GM-CSF基因和IL-6基因,并将GMCSF基因和IL-6基因分别克隆至载体p CDNA3.1-His-C和p ET-32a(+),且诱导表达了IL-6蛋白。将构建的GM-CSF基因的重组载体、诱导表达的IL-6重组蛋白联合畜禽疫病防控技术研发课题组前期构建的TGEV DNA疫苗载体p-N-His分组免疫小鼠(设置p-N-His为对照),运用间接ELISA检测了特异性抗体水平。将构建的重组载体、诱导表达的重组蛋白和PBS分组免疫小鼠,测定了小鼠的生长性能、血清指标和免疫器官指数。结果显示,克隆的GM-CSF和IL-6基因大小分别为579 bp和576 bp,成功构建了真核表达载体p-GM-CSF和原表达载体p ET-IL-6,诱导表达的IL-6重组蛋白大小为38 ku。载体p-GM-CSF、IL-6蛋白联合p-N-His免疫小鼠后,p-N-His+p-GM-CSF、p-N-His+IL-6蛋白和p-N-His均刺激机体产生了特异性抗体,且p-N-His+IL-6蛋白抗体水平高于p-N-His+p-GM-CSF(P<0.05)。IL-6蛋白、p-GM-CSF和PBS分组免疫小鼠后,p-GM-CSF和IL-6蛋白组小鼠的生长性能指标均高于PBS组,且末重、平均日增重、料重比差异显著(P<0.05)。p-GM-CSF、IL-6蛋白、PBS组小鼠的生化指标总蛋白、白蛋白、球蛋白、尿素氮的含量差异均不显著(P>0.05),但IL-6蛋白组小鼠的葡萄糖显著升高(P<0.05),p-GM-CSF、IL-6蛋白、PBS组小鼠的免疫器官指数差异均不显著(P>0.05)。文章以p-N-His为靶载体验证出GM-CSF和IL-6具有较强免疫佐剂活性,且IL-6蛋白的效果强于GM-CSF。p-GM-CSF和IL-6蛋白能够提升小鼠的生长性能指标,对血液指标和免疫器官指数影响不大。 展开更多
关键词 粒细胞-巨噬细胞集落刺激因子 白细胞介素6 免疫佐剂活性 生长性能 血液指标
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联合CSF和DBSCAN的多波束点云水下管线分割方法研究
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作者 沈蔚 杨朝禹 +2 位作者 杨智松 冷佳昕 王梓程 《海洋测绘》 北大核心 2025年第2期24-28,共5页
针对当前水下管线人工识别探测效率低、成本高的问题,利用多波束水深点云,提出一种结合CSF滤波与DBSCAN聚类分割的水下管线自动识别与分割方法。该方法通过CSF滤波将多波束点云分割为水下地形点和管线点,并结合DBSCAN聚类算法进一步过... 针对当前水下管线人工识别探测效率低、成本高的问题,利用多波束水深点云,提出一种结合CSF滤波与DBSCAN聚类分割的水下管线自动识别与分割方法。该方法通过CSF滤波将多波束点云分割为水下地形点和管线点,并结合DBSCAN聚类算法进一步过滤误差点,以获得准确的管线点云。两个实验结果表明,该方法能够准确识别水下管道的三维形态,提取其长度、口径等参数,可以较好的识别水下电缆裸露长度以及走向。同时方法操作简单、易于运用,分割精度高,可大范围应用到水下管线裸露探测与量化评估,为水下管线的管理和保护提供技术方法支持。 展开更多
关键词 管线分割 滤波 布料模拟滤波 DBSCAN聚类 多波束点云
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DTI-ALPS联合gBOLD-CSF技术评估偏头痛患者类淋巴系统功能
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作者 张亚男 刘妮 +7 位作者 韩雪 任蒙蒙 张磊 吴浩然 陈远鹏 郭笑利 刘军莲 霍健伟 《CT理论与应用研究(中英文)》 2025年第6期1092-1098,共7页
目的:探讨无先兆偏头痛(MwoA)患者大脑类淋巴系统功能的变化及其与临床指标的相关性。方法:招募50名MwoA患者及49例匹配的健康对照组。采用3.0T MRI获取影像数据,计算两组沿血管周围间隙的扩散张量成像分析(DTI-ALPS)指数及全局血氧水... 目的:探讨无先兆偏头痛(MwoA)患者大脑类淋巴系统功能的变化及其与临床指标的相关性。方法:招募50名MwoA患者及49例匹配的健康对照组。采用3.0T MRI获取影像数据,计算两组沿血管周围间隙的扩散张量成像分析(DTI-ALPS)指数及全局血氧水平依赖性信号与脑脊液流入动力学之间的耦合强度(gBOLDCSF)的差异,进而评估MwoA患者类淋巴系统的功能变化,并分析其与临床量表评分的相关性。结果:相比健康对照组,MwoA患者左侧DTI-ALPS指数、gBOLD-CSF及-d(gBOLD)/dt-CSF耦合强度明显减低,且患者左侧ALPS指数与贝克焦虑量表评分呈弱负相关。结论:MwoA患者存在大脑类淋巴系统功能障碍,其异常可能参与偏头痛的病理生理过程并与焦虑症状相关,为深入理解偏头痛的发病机制提供新视角。 展开更多
关键词 磁共振 无先兆偏头痛 类淋巴系统 DTI-ALPS指数 gBOLD-csf耦合强度
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CSF-1与CSF-1R在儿童免疫性血小板减少症外周血中的表达及其临床意义
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作者 李丹露 宋海辰 +1 位作者 程永凤 严媚 《中国实验血液学杂志》 北大核心 2025年第4期1131-1137,共7页
目的:探讨CSF-1(集落刺激因子-1)与CSF-1R(集落刺激因子-1受体)在儿童免疫性血小板减少症(ITP)外周血中的表达及其临床意义。方法:选取2023年2月至2024年1月在本院治疗的ITP患儿44例作为观察组,同期选择40例健康儿童作为对照组,收集相... 目的:探讨CSF-1(集落刺激因子-1)与CSF-1R(集落刺激因子-1受体)在儿童免疫性血小板减少症(ITP)外周血中的表达及其临床意义。方法:选取2023年2月至2024年1月在本院治疗的ITP患儿44例作为观察组,同期选择40例健康儿童作为对照组,收集相关临床数据。分离ITP患儿与健康儿童的外周血单个核细胞(PBMC)。采用ELISA法检测两组儿童血浆中M1巨噬细胞相关细胞因子(TNF-α、IL-6),M2巨噬细胞相关细胞因子(IL-10、TGF-β)及CSF-1的含量。RT-PCR法检测两组儿童PBMC中M1巨噬细胞表面标志物(CD86、iNOS),M2巨噬细胞表面标志物(CD206、Arg-1)及CSF-1R的mRNA水平。采用Western blot法检测两组儿童PBMC中CSF-1R蛋白的表达情况。Spearman相关性分析判断CSF-1R与血小板计数、TNF-α、IL-6、IL-10、TGF-β、CSF-1之间的相关性。结果:与对照组相比,ITP患儿血浆中IL-10、TGF-β、CSF-1的含量及血小板计数均显著下降(P<0.01),TNF-α、IL-6的含量显著升高(P<0.01);ITP患儿PBMC中M1巨噬细胞表面标志物(CD86、iNOS)的mRNA水平相对于对照组显著升高(P<0.05),ITP患儿PBMC中M2巨噬细胞表面标志物CD206的mRNA水平相对于对照组下降但无统计学差异(P>0.05),Arg-1的mRNA水平下降差异有统计学意义(P<0.05)。ITP患儿PBMC中CSF-1R的基因、蛋白水平均高于对照组(P<0.01)。CSF-1R在ITP患儿PBMC中的表达与血小板计数、IL-10、CSF-1呈正相关(r=0.822、0.481、0.405)。结论:在ITP患儿血浆中CSF-1含量明显降低,在ITP患儿PBMC中CSF-1R mRNA及蛋白表达显著升高,可能参与调控巨噬细胞M1/M2的失衡,可作为ITP潜在的治疗靶点。 展开更多
关键词 csf-1/csf-1R 原发性免疫性血小板减少症 巨噬细胞极化 M1/M2失衡
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CSF1R^(+/-)小鼠的构建和基因鉴定
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作者 周园园 刘崇 +4 位作者 王安琪 张慧茹 邱佳琪 朱梦娟 涂佳杰 《安徽医科大学学报》 北大核心 2025年第5期884-889,共6页
目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外... 目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外显子下游插入一个双侧有Loxp位点的新霉素抗性盒(PGK-neo)。将线性化的靶向载体电穿孔至胚胎干细胞(ES)。将正确靶向的ES注射到C57BL/6J小鼠的胚泡中得到嵌合小鼠,与透明带3-Cre(Zp3-Cre)小鼠进行繁殖。新生小鼠出生后9~14天编号并剪鼠尾,提取小鼠的DNA,通过聚合酶链式反应(PCR)和琼脂糖凝胶电泳鉴定小鼠基因型,筛选出CSF1R^(+/-)小鼠。应用流式细胞术检测小鼠巨噬细胞中CSF1R的表达;Western blot检测小鼠组织中CSF1R的蛋白表达。结果琼脂糖凝胶电泳结果显示,野生小鼠(WT)扩增出453 bp的条带,CSF1R^(+/-)小鼠扩增出453 bp和650 bp的条带。流式细胞术结果显示,与WT组比较,CSF1R^(+/-)组小鼠的腹腔来源巨噬细胞(PM)和骨髓来源巨噬细胞(BMDM)中的CSF1R低表达(P<0.05)。Western blot结果显示,与WT组比较,CSF1R^(+/-)组小鼠脾脏、肾脏、脑组织中的CSF1R蛋白低表达(P<0.05)。结论成功构建、繁育和鉴定CSF1R^(+/-)小鼠,为进一步揭示CSF1R在免疫调节中的潜在机制研究提供动物模型基础。 展开更多
关键词 csf1R PCR Western blot 流式细胞术 Cre/Loxp 动物模型
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Csf1r^(Cre)介导的黄色荧光素蛋白可有效标记各组织定居巨噬细胞
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作者 李晓宇 赵殿元 +2 位作者 张胜权 杨靖 唐丽 《安徽医科大学学报》 北大核心 2025年第9期1567-1573,共7页
目的构建Csf1r^(Cre)Rosa26^(YFP)报告基因小鼠,检测Csf1r^(Cre)介导黄色荧光素蛋白(YFP)标记单核细胞及不同组织定居巨噬细胞的效率。方法Rosa26^(YFP)突变小鼠具有一个loxP侧翼的STOP序列,后跟插入Rosa26基因座的黄色荧光蛋白基因(YFP... 目的构建Csf1r^(Cre)Rosa26^(YFP)报告基因小鼠,检测Csf1r^(Cre)介导黄色荧光素蛋白(YFP)标记单核细胞及不同组织定居巨噬细胞的效率。方法Rosa26^(YFP)突变小鼠具有一个loxP侧翼的STOP序列,后跟插入Rosa26基因座的黄色荧光蛋白基因(YFP)。当与表达Csf1r^(Cre)重组酶的小鼠交配时,STOP序列被删除,并在双突变后代Csf1r^(Cre)Rosa26^(YFP)的表达组织中观察到YFP表达。将Csf1r^(Cre)小鼠与Rosa26^(YFP)小鼠交配,通过PCR筛选出Csf1r^(Cre)Rosa26^(YFP)报告基因小鼠。分离成年Csf1r^(Cre)Rosa26^(YFP)小鼠的血液及骨髓单核细胞、肝脏巨噬细胞、肾脏巨噬细胞、肺泡巨噬细胞和脾脏巨噬细胞,标记流式抗体,通过流式细胞术分析Csf1r^(Cre)介导YFP标记组织巨噬细胞的效率。结果Csf1r^(Cre)Rosa26^(YFP)报告基因小鼠中肾脏、肝脏、脾脏、肺泡巨噬细胞表达YFP中位数为93.25%、92.45%、91.10%、94.70%,血液单核细胞表达YFP中位数为98.20%,骨髓单核细胞表达YFP中位数为93.90%。结论Csf1r^(Cre)可以介导YFP对各组织定居巨噬细胞以及骨髓和血液单核细胞进行示踪。同时,Csf1r^(Cre)可用作这些细胞的基因条件性敲除工具鼠。 展开更多
关键词 csf1r^(Cre)重组酶 报告基因小鼠 流式细胞术 组织定居巨噬细胞 单核细胞 细胞标记效率
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Long-term combined application of organic and inorganic fertilizers increases crop yield sustainability by improving soil fertility in maize-wheat cropping systems 被引量:2
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作者 Jinfeng Wang Xueyun Yang +3 位作者 Shaomin Huang Lei Wu Zejiang Cai Minggang Xu 《Journal of Integrative Agriculture》 2025年第1期290-305,共16页
Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in t... Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in the long term under the combined application of organic and inorganic fertilizers. Three long-term field trials were conducted to investigate the effects of organic amendments on the grain sustainable yield index(SYI), soil fertility index(SFI)and nutrient balance in maize–wheat cropping systems of central and southern China during 1991–2019. Five treatments were included in the trials: 1) no fertilization(control);2) balanced mineral fertilization(NPK);3) NPK plus manure(NPKM);4) high dose of NPK plus manure(1.5NPKM);and 5) NPK plus crop straw(NPKS). Over time, the grain yields of wheat and maize showed an increasing trend in all four fertilization treatments at the Yangling(YL) and Zhengzhou(ZZ) locations, while they declined at Qiyang(QY). The grain yield in the NPKM and 1.5NPKM treatments gradually exceeded that of the NPK and NPKS treatments at the QY site. The largest SYI was recorded in the NPKM treatment across the three sites, suggesting that inorganic fertilizer combined with manure can effectively improve crop yield sustainability. Higher SYI values were recorded at the YL and ZZ sites than at the QY site, possibly because the soil was more acid at QY. The key factors affecting grain yield were soil available phosphorus(AP) and available potassium(AK) at the YL and ZZ sites, and pH and AP at the QY site.All fertilization treatments resulted in soil N and P surpluses at the three sites, but soil K surpluses were recorded only at the QY site. The SFI was greater in the 1.5NPKM, NPKM and NPKS treatments than in the NPK treatment by 13.3–40.0 and 16.4–63.6% at the YL and ZZ sites, respectively, and was significantly higher in the NPKM and 1.5NPKM treatments than in the NPK and NPKS treatments at the QY site. A significant, positive linear relationship was found between SFI and crop yield, and SYI and nutrient balance, indicating that grain yield and its sustainability significantly increased with increasing soil fertility. The apparent N, P and K balances positively affected SFI.This study suggests that the appropriate amount of manure mixed with mineral NPK fertilizer is beneficial to the development of sustainable agriculture, which effectively increases the crop yield and yield sustainability by improving soil fertility. 展开更多
关键词 organic amendments crop yield yield sustainability soil fertility nutrient balance
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A suitable organic fertilizer substitution ratio stabilizes rainfed maize yields and reduces gaseous nitrogen loss in the Loess Plateau, China 被引量:2
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作者 Lihua Xie Lingling Li +4 位作者 Junhong Xie Jinbin Wang Zechariah Effah Setor Kwami Fudjoe Muhammad Zahid Mumtaz 《Journal of Integrative Agriculture》 2025年第6期2138-2154,共17页
The application of organic fertilizers has become an increasingly popular practice in maize production to reduce thegaseous nitrogen(N) loss and soil degradation caused by inorganic fertilizers. Organic fertilizer pla... The application of organic fertilizers has become an increasingly popular practice in maize production to reduce thegaseous nitrogen(N) loss and soil degradation caused by inorganic fertilizers. Organic fertilizer plays a key rolein improving soil quality and stabilizing maize yields, but few studies have compared different substitution rates. Afield study was carried out in 2021 and 2022, based on a long-term trial initiated in 2016, which included five organicfertilizer N substitution rates with equal inputs of 200 kg N ha^(–1): 0% organic fertilizer(T1, 100% inorganic fertilizer),50.0% organic+50.0% inorganic fertilizer(T2), 37.5% organic+62.5% inorganic fertilizer(T3), 25.0% organic+75.0%inorganic fertilizer(T4), and 12.5% organic+87.5% inorganic fertilizer(T5), as well as a no fertilizer control(T6). Theresults of the two years showed that T3 and T1 had the highest grain yield and biomass, respectively, and there wasno significant difference between T1 and T3. Compared with T1, the 12.5, 25.0, 37.5, and 50.0% substitution rates in T5, T4, T3, and T2 significantly reduced total nitrogen losses(NH_(3), N_(2)O) by 8.3, 16.1, 18.7, and 27.0%, respectively.Nitrogen use efficiency(NUE) was higher in T5, T3, and T1, and there were no significant differences among them.Organic fertilizer substitution directly reduced NH_(3)volatilization and N_(2)O emission from farmland by lowering theammonium nitrogen and alkali-dissolved N contents and by increasing soil moisture. These substitution treatmentsreduced N_(2)O emissions indirectly by regulating the abundances of AOB and nirK-harboring genes by promotingsoil moisture. Specifically, the 37.5% organic fertilizer substitution reduces NH_(3)volatilization and N_(2)O emission from farmland by reducing the ammonium nitrogen and alkali-dissolved N contents and increasing moisture, which negatively regulate the abundance of AOB and nir K-harboring genes to reduce N_(2)O emissions indirectly in rainfed maize fields on the Loess Plateau of China. 展开更多
关键词 organic fertilizer rainfed maize field gaseous nitrogen loss functional gene
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Combining slow-release fertilizer and plastic film mulching reduced the carbon footprint and enhanced maize yield on the Loess Plateau 被引量:2
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作者 Xiaoqing Han Pengfei Dang +12 位作者 Lechen Liao Fangqi Song Miaomiao Zhang Maoxue Zhang Guoqing Li Shuyue Wen Ning Yang Xiping Pan Xiaofan Wang WenWang Xiaoliang Qin Charles O.Joseph Kadambot H.M.Siddique 《Journal of Environmental Sciences》 2025年第1期359-369,共11页
Agricultural practices significantly contribute to greenhouse gas(GHG)emissions,necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production.Plastic film mulc... Agricultural practices significantly contribute to greenhouse gas(GHG)emissions,necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production.Plastic film mulching is commonly used in the Loess Plateau region.Incorporating slow-release fertilizers as a replacement for urea within this practice can reduce nitrogen losses and enhance crop productivity.Combining these techniques represents a novel agricultural approach in semi-arid areas.However,the impact of this integration on soil carbon storage(SOCS),carbon footprint(CF),and economic benefits has received limited research attention.Therefore,we conducted an eight-year study(2015-2022)in the semi-arid northwestern region to quantify the effects of four treatments[urea supplied without plastic film mulching(CK-U),slow-release fertilizer supplied without plastic film mulching(CK-S),urea supplied with plastic film mulching(PM-U),and slow-release fertilizer supplied with plastic film mulching(PM-S)]on soil fertility,economic and environmental benefits.The results revealed that nitrogen fertilizer was the primary contributor to total GHG emissions(≥71.97%).Compared to other treatments,PM-S increased average grain yield by 12.01%-37.89%,water use efficiency by 9.19%-23.33%,nitrogen accumulation by 27.07%-66.19%,and net return by 6.21%-29.57%.Furthermore,PM-S decreased CF by 12.87%-44.31%and CF per net return by 14.25%-41.16%.After eight years,PM-S increased SOCS(0-40 cm)by 2.46%,while PM-U decreased it by 7.09%.These findings highlight the positive effects of PM-S on surface soil fertility,economic gains,and environmental benefits in spring maize production on the Loess Plateau,underscoring its potential for widespread adoption and application. 展开更多
关键词 Plastic film mulching Slow-release fertilizer Maize grain yield Carbon footprint Economic benefits
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PEG-rhG-CSF与rhG-CSF在预防化疗相关中性粒细胞减少症中的疗效与安全性的Meta分析
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作者 张珍珍 王亚华 +3 位作者 刘吉莉 尹博 刘钰彤 焦胜春 《延安大学学报(医学科学版)》 2025年第2期63-72,共10页
目的通过系统评价和Meta分析方法,比较聚乙二醇化重组人粒细胞集落刺激因子(pegylated recombinant human granulocyte colony-stimulating factor,PEG-rhG-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimu... 目的通过系统评价和Meta分析方法,比较聚乙二醇化重组人粒细胞集落刺激因子(pegylated recombinant human granulocyte colony-stimulating factor,PEG-rhG-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)在预防恶性肿瘤化疗相关中性粒细胞减少症中的疗效与安全性差异,为临床合理用药提供循证医学依据。方法本研究采用系统性文献检索策略,全面检索Embase、Web of Science、PubMed、the Cochrane Library、中国知网、万方和维普数据库,收集从建库至2024年8月17日发表的关于PEG-rhG-CSF与rhG-CSF预防恶性肿瘤化疗相关中性粒细胞减少症的随机对照试验。由2名研究者独立进行文献筛选、数据提取及方法学质量评价,采用Cochrane偏倚风险评估工具对纳入研究进行质量评估。使用RevMan 5.4软件进行Meta分析,二分类变量采用风险比及其95%CI表示效应量,连续性变量采用均数差(MD)及其95%CI表示。研究间异质性采用I²统计量进行评估,I²>50%时采用随机效应模型,否则采用固定效应模型。结果共纳入28项随机对照临床试验,涉及2879例恶性肿瘤患者。根据Meta分析结果表明,PEG-rhG-CSF组在减少Ⅲ/Ⅳ度中性粒细胞减少持续时间方面优于rhG-CSF组(P<0.05)。不良反应事件发生率与rhG-CSF组差异没有统计学意义(P>0.05)。结论与rhG-CSF相比,PEG-rhG-CSF可显著缩短恶性肿瘤患者化疗后重度(Ⅲ/Ⅳ度)中性粒细胞减少的持续时间。基于现有循证医学证据,建议在恶性肿瘤化疗患者中优先选用PEG-rhG-CSF进行中性粒细胞减少症的预防,以降低感染风险,保障化疗周期顺利进行并改善患者预后。 展开更多
关键词 聚乙二醇化重组人粒细胞集落刺激因子 重组人粒细胞集落刺激因子 中性粒细胞减少症 META分析
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PEG-rhG-CSF与rhG-CSF预防直肠癌化疗后中性粒细胞减少症的效果比较 被引量:1
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作者 陈世雄 戴俊 张晓明 《中南医学科学杂志》 2025年第4期676-679,共4页
目的比较重组人粒细胞集落刺激因子(rhG-CSF)与聚乙二醇化rhG-CSF(PEG-rhG-CSF)对直肠癌化疗后中性粒细胞减少症的预防效果。方法将92例直肠癌化疗患者随机均分为PEG-rhG-CSF组和rhG-CSF组。rhG-CSF组于化疗后予以rhG-CSF皮下注射,PEG-r... 目的比较重组人粒细胞集落刺激因子(rhG-CSF)与聚乙二醇化rhG-CSF(PEG-rhG-CSF)对直肠癌化疗后中性粒细胞减少症的预防效果。方法将92例直肠癌化疗患者随机均分为PEG-rhG-CSF组和rhG-CSF组。rhG-CSF组于化疗后予以rhG-CSF皮下注射,PEG-rhG-CSF组于化疗后予以PEG-rhG-CSF皮下注射。比较两组Ⅲ、Ⅳ度中性粒细胞减少症发生率、发热性中性粒细胞减少症发生率、抗生素使用率、外周血中性粒细胞绝对值(ANC)变化及不良反应发生情况。结果PEG-rhG-CSF组Ⅲ度及Ⅲ、Ⅳ度中性粒细胞减少症总发生率、发热性中性粒细胞减少症发生率及抗生素使用率均低于rhG-CSF组(P<0.05),ANC恢复时间短于rhG-CSF组(P<0.05)。PEG-rhG-CSF组用药后第1、3、5天ANC均高于rhG-CSF组(P<0.05)。PEG-rhG-CSF组和rhG-CSF组用药期间不良反应发生率比较,差异无统计学意义(P>0.05)。结论相较于rhG-CSF,直肠癌化疗后使用PEG-rhG-CSF预防中性粒细胞减少症效果更优,能够更有效降低Ⅲ度及以上中性粒细胞减少症发生率,缩短ANC恢复时间,减少发热性中性粒细胞减少症的发生率以及抗生素的使用率,且两者安全性差异无显著性。 展开更多
关键词 直肠癌 聚乙二醇化重组人粒细胞集落刺激因子 重组人粒细胞集落刺激因子 中性粒细胞减少症
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Assessing the effect of composted cyclosporin A fermentation residue as organic fertilizer:Focus on soil fertility and antibiotic resistance 被引量:1
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作者 Dong Wang Xiang Chen +9 位作者 Xiaofen Li Qingwen Zhang Jiaqi Hou Yuefei Li Beidou Xi Zhihao Sun Shuaishuai Xin Guocheng Liu Huiling Liu Yanjun Xin 《Journal of Environmental Sciences》 2025年第11期793-803,共11页
Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropria... Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropriate handling of CFR not only waste valuable bioresources,but may also lead to the cyclosporin A and associated resistance genes into the natural environment,posing a significant threat to ecological system and human health.Land application was an effective way to resource recovery of CFR after aerobic composting(CAC).This study investigated the impact of CAC on soil fertility and environmental safety.The results indicated that CAC could improve soil nutrient contents and enhance enzyme activities.CAC altered the diversity and community composition of soil bacteria,resulting in an increase in the abundance of relevant bacteria beneficial for organic matter decomposition and cyclosporin A degradation.The introduced cyclosporin A(71.69μg/kg)completely degraded within 20 days due to soil biodegradation.The significantly increased abundance of intIl,mdr3,pgp,TSR and pmra in the soil cultivation early stage were restored to the soil background level within 90 days,indicating a reduced risk of antimicrobial resistance.The results demonstrated that reasonable land application of CAC could improve soil fertility without antimicrobial resistance risk,which is helpful for evaluating the resource utilization value and environmental risks of antibiotic fermentation residue after aerobic composting. 展开更多
关键词 Cyclosporin A Antibiotic fermentation residue Soil fertility Ntibiotic resistance gene
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Organic fertilizer enhances soil aggregate stability by altering greenhouse soil content of iron oxide and organic carbon 被引量:1
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作者 Lijun Ren Han Yang +4 位作者 Jin Li Nan Zhang Yanyu Han Hongtao Zou Yulong Zhang 《Journal of Integrative Agriculture》 2025年第1期306-321,共16页
Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil aggregates.However, the mechanism of these drivers in greenhouse soil fertilized with organic ... Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil aggregates.However, the mechanism of these drivers in greenhouse soil fertilized with organic fertilizer is not well understood.In a 3-year field experiment, we aimed to investigate the factors which drive the stability of soil aggregates in greenhouse soil.To explore the impact of organic fertilizer on soil aggregates, we established four treatments:no fertilization (CK);inorganic fertilizer (CF);organic fertilizer (OF);and combined application of inorganic and organic fertilizers(COF).The application of organic fertilizer significantly enhanced the stability of aggregates, that is it enhanced the mean weight diameter, geometric mean diameter and aggregate content (%) of>0.25 mm aggregate fractions.OF and COF treatments increased the concentration of SOC, especially the aliphatic-C, aromatic-C and polysaccharide-C components of SOC, particularly in>0.25 mm aggregates.Organic fertilizer application significantly increased the content of free Fe(Fed), reactive Fe (Feo), and non-crystalline Fe in both bulk soil and aggregates.Furthermore, non-crystalline Fe showed a positive correlation with SOC content in both bulk soil and aggregates.Both non-crystalline Fe and SOC were significantly positively correlated with>2 mm mean weight diameter.Overall, we believe that the increase of SOC, aromatic-C, and non-crystal ine Fe concentrations in soil after the application of organic fertilizer is the reason for improving soil aggregate stability. 展开更多
关键词 organic fertilizer soil aggregates soil organic carbon iron oxides greenhouse soil
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The role of silica in biomass for calcium-modified biochar:Phosphorus removal mechanism and potential as a phosphate fertilizer application 被引量:1
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作者 Yongyi Chen Ruiling Zhang +7 位作者 Jiayong Gao Mujun Han Songyan Qin Kai Liu Yajie Shu Ruirui Zhang Chang Shi Yue Zheng 《Journal of Environmental Sciences》 2025年第12期242-253,共12页
The interaction mechanism between eggshell calcium and endogenous silica in biomass during biochar modification,and its impact on phosphate adsorption performance and slow-release fertilizer characteristics,remains un... The interaction mechanism between eggshell calcium and endogenous silica in biomass during biochar modification,and its impact on phosphate adsorption performance and slow-release fertilizer characteristics,remains unexplored.This study investigates that high silica content in biomass(>6%)inhibits the decomposition of CaCO_(3)in eggshells during pyrolysis,reducing the formation of active calcium species(CaO and Ca(OH)_(2)),while moderate silica levels(4%-5%)promote the formation of CaSiO_(3),enhancing phosphorus adsorption without hindering Ca^(2+)activation.Adsorption studies reveal that the precipitation of Ca_(5)(PO_(4))_(3)(OH)resulting from the combination of CaO and Ca(OH)_(2)with phosphate is the primary and effective form for phosphorus removal in calcium-modified adsorbents,accompanied by Ca_(3)(PO_(4))_(2)·2H_(2)O precipitation formed by CaSi O_(3).Eggshell calcium-modified corn straw biochar(ECS)exhibited the highest adsorption capacity,reaching 123.3 mg/g,outperforming materials in previous studies.ECS also demonstrated excellent pH adaptability and selective phosphate removal.As a biochar-based phosphorus fertilizer,ECS-P exhibits high phosphorus extractability in formic acid(93.92%)but low water solubility(0.62%),with phosphorus release during the seven-day intermittent leaching experiment remaining between 0.53 to 0.875 mg/L.These results confirm its potential as a phosphorus cycling fertilizer.This study provides fundamental insights into optimizing biomass selection based on silica content for calcium modification,offering an efficient strategy for both phosphate recovery and slow-release fertilizer development. 展开更多
关键词 Silicon-rich biomass Waste resources Recycling phosphorus Calcium-modified biochar Slow-release phosphate fertilizer
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G-CSF通过调控M2巨噬细胞极化和MAPK-ERK信号改善大鼠薄型子宫内膜
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作者 王珍 武依娜 +2 位作者 汤美玲 周青青 周晓燕 《中国优生与遗传杂志》 2025年第7期1501-1508,共8页
目的 薄型子宫内膜是女性不孕和植入失败的重要病因,本研究旨在探讨粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)通过调控M2型巨噬细胞及MAPK-ERK信号通路对薄型子宫内膜增厚的作用及其潜在机制。方法 通过注射无... 目的 薄型子宫内膜是女性不孕和植入失败的重要病因,本研究旨在探讨粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)通过调控M2型巨噬细胞及MAPK-ERK信号通路对薄型子宫内膜增厚的作用及其潜在机制。方法 通过注射无水乙醇法建立大鼠薄型子宫内膜模型,分为对照组、模型组及模型+G-CSF组。采用H-E染色评估子宫内膜厚度及纤维化程度,利用免疫组化及免疫荧光检测增殖及血管生成标志物抗原Ki-67(antigen Ki-67,Ki67)和血管性血友病因子(Von Willebrand factor, vWF)的表达。通过流式细胞术分析M2型巨噬细胞比例及白细胞介素-4(interleukin-4,IL-4)和白细胞介素-10(interleukin-10,IL-10),并通过Western bloting检测MAPK-ERK信号通路磷酸化细胞外信号调节激酶1/2(phosphorylated extracellular signal-regulated kinase 1/2,p-ERK1/2)和磷酸化p38丝裂原活化蛋白激酶(phosphorylated p38 mitogen-activated protein kinase,p-p38)蛋白的表达水平。结果 G-CSF显著改善薄型子宫内膜厚度及组织结构,减少纤维化程度,增加Ki67阳性细胞比例及血管生成标志物表达水平。流式细胞术结果显示,G-CSF处理组M2型巨噬细胞CD206^(+)/CD11c^(+)比例及IL-4和IL-10抗炎因子水平显著升高。Western bloting结果显示,G-CSF通过激活MAPK-ERK信号通路,促进内膜细胞增殖及血管生成。结论 G-CSF通过调控M2型巨噬细胞极化及MAPK-ERK信号通路,显著改善薄型子宫内膜厚度,促进细胞增殖及血管生成。本研究为G-CSF在薄型子宫内膜治疗中的应用提供了新证据。 展开更多
关键词 粒细胞集落刺激因子 M2型巨噬细胞 MAPK-ERK信号通路 薄型子宫内膜
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Effect of Tithonia diversifolia (Hemsley) and Inorganic Fertilizers on Morpho-Agronomic Characteristics of Rice (Oryza sativa L.) Grown on Oxisols in Democratic Republic of Congo
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作者 Constantin Kalubi Nkongolo Georges Mupala Muyayabantu André Mbumba Kayombo 《American Journal of Plant Sciences》 2025年第1期64-75,共12页
Rice (Oryza sativa) is becoming a staplefood in many regions of DR-Congo. However, its production is still limited to the North Eastern part of the country and grain yield is low. A study was carried out in the city o... Rice (Oryza sativa) is becoming a staplefood in many regions of DR-Congo. However, its production is still limited to the North Eastern part of the country and grain yield is low. A study was carried out in the city of Mbujimayi located in the Central part of the DR-Congo to assess the effects of organic and inorganic fertilizers on morpho-agronomic characteristics of O. sativa. The trial was conducted during the 2021 agricultural season A using a completely randomized design with three replicates. The six treatments studied consisted of application of T. diversifolia biomass at a dose of 2 kg/4m2 (BFT − 2 kg), 4 kg/4 m2 (BFT − 4 kg), inorganic fertilizer consisting with NPK17-17-17 + Urea (46% N) at a combined dose of 80 g/4 m2 (NP), 1/2 of the combination (BFT − 2 kg + NP) and finally 1/2 of the combination (BFT − 4 kg + NP). The untreated plots were used as controls. Plants treated with 1/2 combination (BFT − 4 kg + NP), BFT – 4 kg, and NP showed similar height (100.93 cm, 99.03 cm, and 98.63 cm, respectively) that were significantly higher than control and other treatments [1/2 (BFT – 2 kg + NP, BFT – 2 kg] For agronomic characteristics, days to 50% flowering varied between 73.00 and 74 days with an average of 74 days. The control and BFT – 4 kg showed significantly shorter panicles compared to other treatments. For yield components, 1/2 (BFT – 4 kg + NP) and the NP treatments generated a higher weight of 1000 grains. For yield per hectare, 1/2 (BFT − 4 kg + NP) induced significantly different levels of production than the control and other treatments, including 1/2 (BFT – 2 kg + NP), BFT – 4 kg + NP, BFT – 2 kg, BFT – 4 kg. The correlation coefficients between agronomic traits revealed that with the exception of the length of particle and the abortion rates, all the yield components (panicles per plant, seeds per panicle, weight of 1000 grains, and grail yield per plot) were strongly correlated with grain yield per hectare. 展开更多
关键词 RICE FERTILIZATION Mineral fertilizer Organic fertilizer YIELD
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基于CSF的建筑物点云特征提取实现
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作者 刘致远 梁静 《城市勘测》 2025年第3期23-28,共6页
当前特征点的选取大多基于单一微分特性等方法对点云数据的特征点直接提取,存在受点云密度、复杂场景干扰导致错提、漏提的不足。本文提出基于CSF的点云滤波与微分信息特征提取方法实现单体建筑物点云轮廓特征的提取。试验结果表明,滤... 当前特征点的选取大多基于单一微分特性等方法对点云数据的特征点直接提取,存在受点云密度、复杂场景干扰导致错提、漏提的不足。本文提出基于CSF的点云滤波与微分信息特征提取方法实现单体建筑物点云轮廓特征的提取。试验结果表明,滤波后的单体建筑物点云在4组特征提取阈值下的结果对比,当阈值为0.07时,所提取特征点云相对更能很好地反映出单体建筑物原始点云的特征轮廓,共提取轮廓特征点369001个,剔除率93.86%。 展开更多
关键词 csf PCA 微分信息 特征提取
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Profitability of Fertilizer Use for Citrus Production in Teso Sub-Region, Eastern Uganda
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作者 Onesmus Semalulu Patrick Makhosi +9 位作者 Edgar Samuel Tinyiro Isaac Obongo Patricia Driciru Doreen Namamya Park Taeseon Cho Ilho Caroline Asio Bernard Opio Emannuel Ikwap John Adriko 《Agricultural Sciences》 2025年第1期215-225,共11页
Citrus is an important commercial crop in Uganda, especially the Eastern region. However, in spite of the increasing regional demand, citrus productivity is still low, attributed to pest and diseases, soil moisture st... Citrus is an important commercial crop in Uganda, especially the Eastern region. However, in spite of the increasing regional demand, citrus productivity is still low, attributed to pest and diseases, soil moisture stress, and low soil fertility, among others. Efforts to improve soil fertility are limited by inadequate supply of organic fertilizers due to competing demands. In addition, there is inadequate information on inorganic fertilizer requirements for citrus production in Uganda. The objective of this study was to develop optimum fertilizer recommendations for citrus production for Eastern Uganda. The study was conducted in Teso region, Eastern Uganda. Fertilizer (NPK, 17:17:17) was randomly applied to Hamlin, Valencia and Washington varieties with fertilizer and variety factorially arranged for each farm and citrus age range, replicated three times. Fertilizer rates were 0, 139, 278 and 556 kg NPK/ha for the 4 - 7-year old trees, and 0, 278, 556 and 1111 kg NPK/ha for the mature (8 years and above) trees. For a given variety, each fertilizer rate was applied onto three representative trees per farmer, six farmers per district. Results showed that yields and net profits were highest for variety Hamlin, and nearly the same for varieties Washington and Valencia. Fertilizer application increased fruit yield and profits for both the 4 to 7-year and 8 and above-year-old trees, with highest yield and profitability values observed at 556 kg NPK/ha. These results suggest applying 556 kg NPK/ha to citrus per year as an optimum fertilizer rate for citrus production in Teso region. The fertilizer should be applied in smaller splits of 800, 600, and 600 grams per tree, applied in April, June, and August. 展开更多
关键词 Citrus Varieties Climate-Smart Agriculture COST-EFFECTIVENESS fertilizer Recommendations Soil Fertility Management
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