Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassi...Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.展开更多
Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in t...Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in the long term under the combined application of organic and inorganic fertilizers. Three long-term field trials were conducted to investigate the effects of organic amendments on the grain sustainable yield index(SYI), soil fertility index(SFI)and nutrient balance in maize–wheat cropping systems of central and southern China during 1991–2019. Five treatments were included in the trials: 1) no fertilization(control);2) balanced mineral fertilization(NPK);3) NPK plus manure(NPKM);4) high dose of NPK plus manure(1.5NPKM);and 5) NPK plus crop straw(NPKS). Over time, the grain yields of wheat and maize showed an increasing trend in all four fertilization treatments at the Yangling(YL) and Zhengzhou(ZZ) locations, while they declined at Qiyang(QY). The grain yield in the NPKM and 1.5NPKM treatments gradually exceeded that of the NPK and NPKS treatments at the QY site. The largest SYI was recorded in the NPKM treatment across the three sites, suggesting that inorganic fertilizer combined with manure can effectively improve crop yield sustainability. Higher SYI values were recorded at the YL and ZZ sites than at the QY site, possibly because the soil was more acid at QY. The key factors affecting grain yield were soil available phosphorus(AP) and available potassium(AK) at the YL and ZZ sites, and pH and AP at the QY site.All fertilization treatments resulted in soil N and P surpluses at the three sites, but soil K surpluses were recorded only at the QY site. The SFI was greater in the 1.5NPKM, NPKM and NPKS treatments than in the NPK treatment by 13.3–40.0 and 16.4–63.6% at the YL and ZZ sites, respectively, and was significantly higher in the NPKM and 1.5NPKM treatments than in the NPK and NPKS treatments at the QY site. A significant, positive linear relationship was found between SFI and crop yield, and SYI and nutrient balance, indicating that grain yield and its sustainability significantly increased with increasing soil fertility. The apparent N, P and K balances positively affected SFI.This study suggests that the appropriate amount of manure mixed with mineral NPK fertilizer is beneficial to the development of sustainable agriculture, which effectively increases the crop yield and yield sustainability by improving soil fertility.展开更多
The application of organic fertilizers has become an increasingly popular practice in maize production to reduce thegaseous nitrogen(N) loss and soil degradation caused by inorganic fertilizers. Organic fertilizer pla...The application of organic fertilizers has become an increasingly popular practice in maize production to reduce thegaseous nitrogen(N) loss and soil degradation caused by inorganic fertilizers. Organic fertilizer plays a key rolein improving soil quality and stabilizing maize yields, but few studies have compared different substitution rates. Afield study was carried out in 2021 and 2022, based on a long-term trial initiated in 2016, which included five organicfertilizer N substitution rates with equal inputs of 200 kg N ha^(–1): 0% organic fertilizer(T1, 100% inorganic fertilizer),50.0% organic+50.0% inorganic fertilizer(T2), 37.5% organic+62.5% inorganic fertilizer(T3), 25.0% organic+75.0%inorganic fertilizer(T4), and 12.5% organic+87.5% inorganic fertilizer(T5), as well as a no fertilizer control(T6). Theresults of the two years showed that T3 and T1 had the highest grain yield and biomass, respectively, and there wasno significant difference between T1 and T3. Compared with T1, the 12.5, 25.0, 37.5, and 50.0% substitution rates in T5, T4, T3, and T2 significantly reduced total nitrogen losses(NH_(3), N_(2)O) by 8.3, 16.1, 18.7, and 27.0%, respectively.Nitrogen use efficiency(NUE) was higher in T5, T3, and T1, and there were no significant differences among them.Organic fertilizer substitution directly reduced NH_(3)volatilization and N_(2)O emission from farmland by lowering theammonium nitrogen and alkali-dissolved N contents and by increasing soil moisture. These substitution treatmentsreduced N_(2)O emissions indirectly by regulating the abundances of AOB and nirK-harboring genes by promotingsoil moisture. Specifically, the 37.5% organic fertilizer substitution reduces NH_(3)volatilization and N_(2)O emission from farmland by reducing the ammonium nitrogen and alkali-dissolved N contents and increasing moisture, which negatively regulate the abundance of AOB and nir K-harboring genes to reduce N_(2)O emissions indirectly in rainfed maize fields on the Loess Plateau of China.展开更多
Agricultural practices significantly contribute to greenhouse gas(GHG)emissions,necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production.Plastic film mulc...Agricultural practices significantly contribute to greenhouse gas(GHG)emissions,necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production.Plastic film mulching is commonly used in the Loess Plateau region.Incorporating slow-release fertilizers as a replacement for urea within this practice can reduce nitrogen losses and enhance crop productivity.Combining these techniques represents a novel agricultural approach in semi-arid areas.However,the impact of this integration on soil carbon storage(SOCS),carbon footprint(CF),and economic benefits has received limited research attention.Therefore,we conducted an eight-year study(2015-2022)in the semi-arid northwestern region to quantify the effects of four treatments[urea supplied without plastic film mulching(CK-U),slow-release fertilizer supplied without plastic film mulching(CK-S),urea supplied with plastic film mulching(PM-U),and slow-release fertilizer supplied with plastic film mulching(PM-S)]on soil fertility,economic and environmental benefits.The results revealed that nitrogen fertilizer was the primary contributor to total GHG emissions(≥71.97%).Compared to other treatments,PM-S increased average grain yield by 12.01%-37.89%,water use efficiency by 9.19%-23.33%,nitrogen accumulation by 27.07%-66.19%,and net return by 6.21%-29.57%.Furthermore,PM-S decreased CF by 12.87%-44.31%and CF per net return by 14.25%-41.16%.After eight years,PM-S increased SOCS(0-40 cm)by 2.46%,while PM-U decreased it by 7.09%.These findings highlight the positive effects of PM-S on surface soil fertility,economic gains,and environmental benefits in spring maize production on the Loess Plateau,underscoring its potential for widespread adoption and application.展开更多
目的通过系统评价和Meta分析方法,比较聚乙二醇化重组人粒细胞集落刺激因子(pegylated recombinant human granulocyte colony-stimulating factor,PEG-rhG-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimu...目的通过系统评价和Meta分析方法,比较聚乙二醇化重组人粒细胞集落刺激因子(pegylated recombinant human granulocyte colony-stimulating factor,PEG-rhG-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)在预防恶性肿瘤化疗相关中性粒细胞减少症中的疗效与安全性差异,为临床合理用药提供循证医学依据。方法本研究采用系统性文献检索策略,全面检索Embase、Web of Science、PubMed、the Cochrane Library、中国知网、万方和维普数据库,收集从建库至2024年8月17日发表的关于PEG-rhG-CSF与rhG-CSF预防恶性肿瘤化疗相关中性粒细胞减少症的随机对照试验。由2名研究者独立进行文献筛选、数据提取及方法学质量评价,采用Cochrane偏倚风险评估工具对纳入研究进行质量评估。使用RevMan 5.4软件进行Meta分析,二分类变量采用风险比及其95%CI表示效应量,连续性变量采用均数差(MD)及其95%CI表示。研究间异质性采用I²统计量进行评估,I²>50%时采用随机效应模型,否则采用固定效应模型。结果共纳入28项随机对照临床试验,涉及2879例恶性肿瘤患者。根据Meta分析结果表明,PEG-rhG-CSF组在减少Ⅲ/Ⅳ度中性粒细胞减少持续时间方面优于rhG-CSF组(P<0.05)。不良反应事件发生率与rhG-CSF组差异没有统计学意义(P>0.05)。结论与rhG-CSF相比,PEG-rhG-CSF可显著缩短恶性肿瘤患者化疗后重度(Ⅲ/Ⅳ度)中性粒细胞减少的持续时间。基于现有循证医学证据,建议在恶性肿瘤化疗患者中优先选用PEG-rhG-CSF进行中性粒细胞减少症的预防,以降低感染风险,保障化疗周期顺利进行并改善患者预后。展开更多
Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropria...Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropriate handling of CFR not only waste valuable bioresources,but may also lead to the cyclosporin A and associated resistance genes into the natural environment,posing a significant threat to ecological system and human health.Land application was an effective way to resource recovery of CFR after aerobic composting(CAC).This study investigated the impact of CAC on soil fertility and environmental safety.The results indicated that CAC could improve soil nutrient contents and enhance enzyme activities.CAC altered the diversity and community composition of soil bacteria,resulting in an increase in the abundance of relevant bacteria beneficial for organic matter decomposition and cyclosporin A degradation.The introduced cyclosporin A(71.69μg/kg)completely degraded within 20 days due to soil biodegradation.The significantly increased abundance of intIl,mdr3,pgp,TSR and pmra in the soil cultivation early stage were restored to the soil background level within 90 days,indicating a reduced risk of antimicrobial resistance.The results demonstrated that reasonable land application of CAC could improve soil fertility without antimicrobial resistance risk,which is helpful for evaluating the resource utilization value and environmental risks of antibiotic fermentation residue after aerobic composting.展开更多
Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil aggregates.However, the mechanism of these drivers in greenhouse soil fertilized with organic ...Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil aggregates.However, the mechanism of these drivers in greenhouse soil fertilized with organic fertilizer is not well understood.In a 3-year field experiment, we aimed to investigate the factors which drive the stability of soil aggregates in greenhouse soil.To explore the impact of organic fertilizer on soil aggregates, we established four treatments:no fertilization (CK);inorganic fertilizer (CF);organic fertilizer (OF);and combined application of inorganic and organic fertilizers(COF).The application of organic fertilizer significantly enhanced the stability of aggregates, that is it enhanced the mean weight diameter, geometric mean diameter and aggregate content (%) of>0.25 mm aggregate fractions.OF and COF treatments increased the concentration of SOC, especially the aliphatic-C, aromatic-C and polysaccharide-C components of SOC, particularly in>0.25 mm aggregates.Organic fertilizer application significantly increased the content of free Fe(Fed), reactive Fe (Feo), and non-crystalline Fe in both bulk soil and aggregates.Furthermore, non-crystalline Fe showed a positive correlation with SOC content in both bulk soil and aggregates.Both non-crystalline Fe and SOC were significantly positively correlated with>2 mm mean weight diameter.Overall, we believe that the increase of SOC, aromatic-C, and non-crystal ine Fe concentrations in soil after the application of organic fertilizer is the reason for improving soil aggregate stability.展开更多
The interaction mechanism between eggshell calcium and endogenous silica in biomass during biochar modification,and its impact on phosphate adsorption performance and slow-release fertilizer characteristics,remains un...The interaction mechanism between eggshell calcium and endogenous silica in biomass during biochar modification,and its impact on phosphate adsorption performance and slow-release fertilizer characteristics,remains unexplored.This study investigates that high silica content in biomass(>6%)inhibits the decomposition of CaCO_(3)in eggshells during pyrolysis,reducing the formation of active calcium species(CaO and Ca(OH)_(2)),while moderate silica levels(4%-5%)promote the formation of CaSiO_(3),enhancing phosphorus adsorption without hindering Ca^(2+)activation.Adsorption studies reveal that the precipitation of Ca_(5)(PO_(4))_(3)(OH)resulting from the combination of CaO and Ca(OH)_(2)with phosphate is the primary and effective form for phosphorus removal in calcium-modified adsorbents,accompanied by Ca_(3)(PO_(4))_(2)·2H_(2)O precipitation formed by CaSi O_(3).Eggshell calcium-modified corn straw biochar(ECS)exhibited the highest adsorption capacity,reaching 123.3 mg/g,outperforming materials in previous studies.ECS also demonstrated excellent pH adaptability and selective phosphate removal.As a biochar-based phosphorus fertilizer,ECS-P exhibits high phosphorus extractability in formic acid(93.92%)but low water solubility(0.62%),with phosphorus release during the seven-day intermittent leaching experiment remaining between 0.53 to 0.875 mg/L.These results confirm its potential as a phosphorus cycling fertilizer.This study provides fundamental insights into optimizing biomass selection based on silica content for calcium modification,offering an efficient strategy for both phosphate recovery and slow-release fertilizer development.展开更多
Rice (Oryza sativa) is becoming a staplefood in many regions of DR-Congo. However, its production is still limited to the North Eastern part of the country and grain yield is low. A study was carried out in the city o...Rice (Oryza sativa) is becoming a staplefood in many regions of DR-Congo. However, its production is still limited to the North Eastern part of the country and grain yield is low. A study was carried out in the city of Mbujimayi located in the Central part of the DR-Congo to assess the effects of organic and inorganic fertilizers on morpho-agronomic characteristics of O. sativa. The trial was conducted during the 2021 agricultural season A using a completely randomized design with three replicates. The six treatments studied consisted of application of T. diversifolia biomass at a dose of 2 kg/4m2 (BFT − 2 kg), 4 kg/4 m2 (BFT − 4 kg), inorganic fertilizer consisting with NPK17-17-17 + Urea (46% N) at a combined dose of 80 g/4 m2 (NP), 1/2 of the combination (BFT − 2 kg + NP) and finally 1/2 of the combination (BFT − 4 kg + NP). The untreated plots were used as controls. Plants treated with 1/2 combination (BFT − 4 kg + NP), BFT – 4 kg, and NP showed similar height (100.93 cm, 99.03 cm, and 98.63 cm, respectively) that were significantly higher than control and other treatments [1/2 (BFT – 2 kg + NP, BFT – 2 kg] For agronomic characteristics, days to 50% flowering varied between 73.00 and 74 days with an average of 74 days. The control and BFT – 4 kg showed significantly shorter panicles compared to other treatments. For yield components, 1/2 (BFT – 4 kg + NP) and the NP treatments generated a higher weight of 1000 grains. For yield per hectare, 1/2 (BFT − 4 kg + NP) induced significantly different levels of production than the control and other treatments, including 1/2 (BFT – 2 kg + NP), BFT – 4 kg + NP, BFT – 2 kg, BFT – 4 kg. The correlation coefficients between agronomic traits revealed that with the exception of the length of particle and the abortion rates, all the yield components (panicles per plant, seeds per panicle, weight of 1000 grains, and grail yield per plot) were strongly correlated with grain yield per hectare.展开更多
Citrus is an important commercial crop in Uganda, especially the Eastern region. However, in spite of the increasing regional demand, citrus productivity is still low, attributed to pest and diseases, soil moisture st...Citrus is an important commercial crop in Uganda, especially the Eastern region. However, in spite of the increasing regional demand, citrus productivity is still low, attributed to pest and diseases, soil moisture stress, and low soil fertility, among others. Efforts to improve soil fertility are limited by inadequate supply of organic fertilizers due to competing demands. In addition, there is inadequate information on inorganic fertilizer requirements for citrus production in Uganda. The objective of this study was to develop optimum fertilizer recommendations for citrus production for Eastern Uganda. The study was conducted in Teso region, Eastern Uganda. Fertilizer (NPK, 17:17:17) was randomly applied to Hamlin, Valencia and Washington varieties with fertilizer and variety factorially arranged for each farm and citrus age range, replicated three times. Fertilizer rates were 0, 139, 278 and 556 kg NPK/ha for the 4 - 7-year old trees, and 0, 278, 556 and 1111 kg NPK/ha for the mature (8 years and above) trees. For a given variety, each fertilizer rate was applied onto three representative trees per farmer, six farmers per district. Results showed that yields and net profits were highest for variety Hamlin, and nearly the same for varieties Washington and Valencia. Fertilizer application increased fruit yield and profits for both the 4 to 7-year and 8 and above-year-old trees, with highest yield and profitability values observed at 556 kg NPK/ha. These results suggest applying 556 kg NPK/ha to citrus per year as an optimum fertilizer rate for citrus production in Teso region. The fertilizer should be applied in smaller splits of 800, 600, and 600 grams per tree, applied in April, June, and August.展开更多
基金funded by the National Natural Science Foundation of China (31760363)the Earmarked Fund for CARS (CARS-14-1-16)+1 种基金the Gansu Education Science and Technology Innovation Industry Support Program,China (2021CYZC-38)the Gansu Provincial Key Laboratory of Arid Land Crop Science,Gansu Agricultural University,China (GSCS-2020-Z6)。
文摘Lodging is a major constraint limiting oil flax production efficiency in northern China.Crop lodging susceptibility is closely related to stem lignin content,and the regulatory mechanisms by which nitrogen and potassium fertilization interactively influence lignin biosynthesis in oil flax stems require further investigation.Therefore,this study aimed to enhance lodging resistance and increase grain yield in oil flax.We examined the interactive effects of different nitrogen (75,150,and 225 kg N ha^(–1)) and potassium (60 and 90 kg K_(2)O ha^(–1)) fertilizer rates on lignin metabolism,lodging resistance,and grain yield during the 2022 and 2023 growing seasons.Results indicated that nitrogen and potassium fertilizer levels and their interactions promoted lignin accumulation,improved lodging resistance,and increased grain yield.Compared to the control (CK),the75–150 kg N ha^(–1) combined with 60 kg K_(2)O ha^(–1) treatments significantly enhanced the activities of key lignin-synthesizing enzymes (tyrosine ammonia-lyase (TAL),phenylalanine ammonia-lyase (PAL),cinnamyl alcohol dehydrogenase (CAD),and peroxidase (POD)) and upregulated the expression of 4CL1 and F5H3 genes,leading to a 29.63–43.30%increase in lignin content,improved stem bending strength and lodging resistance index,and a 23.27–32.34%increase in grain yield.Correlation analysis revealed that nitrogen and potassium fertilizers positively regulated enzyme activities and gene expression related to lignin biosynthesis,thereby facilitating lignin accumulation and enhancing stem mechanical strength and lodging resistance.Positive correlations were observed among lignin-related enzyme activities,gene expression,lodging resistance traits,and grain yield.In summary,the application of 75–150 kg N ha^(–1) in conjunction with 60 kg K_(2)O ha^(–1)promoted lignin biosynthesis and accumulation,enhanced lodging resistance,and increased grain yield in oil flax grown in the dryland farming region of central Gansu,China.Furthermore,this treatment provides a technical basis for cultivating stress-tolerant and high-yield oil flax in arid regions.
基金supported by the National Natural Science Foundation of China(42177341)the Natural Science Basic Research Program of Shanxi,China(202203021222138).
文摘Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in the long term under the combined application of organic and inorganic fertilizers. Three long-term field trials were conducted to investigate the effects of organic amendments on the grain sustainable yield index(SYI), soil fertility index(SFI)and nutrient balance in maize–wheat cropping systems of central and southern China during 1991–2019. Five treatments were included in the trials: 1) no fertilization(control);2) balanced mineral fertilization(NPK);3) NPK plus manure(NPKM);4) high dose of NPK plus manure(1.5NPKM);and 5) NPK plus crop straw(NPKS). Over time, the grain yields of wheat and maize showed an increasing trend in all four fertilization treatments at the Yangling(YL) and Zhengzhou(ZZ) locations, while they declined at Qiyang(QY). The grain yield in the NPKM and 1.5NPKM treatments gradually exceeded that of the NPK and NPKS treatments at the QY site. The largest SYI was recorded in the NPKM treatment across the three sites, suggesting that inorganic fertilizer combined with manure can effectively improve crop yield sustainability. Higher SYI values were recorded at the YL and ZZ sites than at the QY site, possibly because the soil was more acid at QY. The key factors affecting grain yield were soil available phosphorus(AP) and available potassium(AK) at the YL and ZZ sites, and pH and AP at the QY site.All fertilization treatments resulted in soil N and P surpluses at the three sites, but soil K surpluses were recorded only at the QY site. The SFI was greater in the 1.5NPKM, NPKM and NPKS treatments than in the NPK treatment by 13.3–40.0 and 16.4–63.6% at the YL and ZZ sites, respectively, and was significantly higher in the NPKM and 1.5NPKM treatments than in the NPK and NPKS treatments at the QY site. A significant, positive linear relationship was found between SFI and crop yield, and SYI and nutrient balance, indicating that grain yield and its sustainability significantly increased with increasing soil fertility. The apparent N, P and K balances positively affected SFI.This study suggests that the appropriate amount of manure mixed with mineral NPK fertilizer is beneficial to the development of sustainable agriculture, which effectively increases the crop yield and yield sustainability by improving soil fertility.
基金supported by the State Key Laboratory of Arid Land Crop Science, Gansu Agricultural University,China (GSCS-2022-Z02)the National Key R&D Program of China (2022YFD1900300)+2 种基金the National Natural Science Foundation of China (32260549)the Innovation Group of Basic Research in Gansu Province, China (25JRRA807)the Major Special Research Projects in Gansu Province, China (22ZD6NA009)。
文摘The application of organic fertilizers has become an increasingly popular practice in maize production to reduce thegaseous nitrogen(N) loss and soil degradation caused by inorganic fertilizers. Organic fertilizer plays a key rolein improving soil quality and stabilizing maize yields, but few studies have compared different substitution rates. Afield study was carried out in 2021 and 2022, based on a long-term trial initiated in 2016, which included five organicfertilizer N substitution rates with equal inputs of 200 kg N ha^(–1): 0% organic fertilizer(T1, 100% inorganic fertilizer),50.0% organic+50.0% inorganic fertilizer(T2), 37.5% organic+62.5% inorganic fertilizer(T3), 25.0% organic+75.0%inorganic fertilizer(T4), and 12.5% organic+87.5% inorganic fertilizer(T5), as well as a no fertilizer control(T6). Theresults of the two years showed that T3 and T1 had the highest grain yield and biomass, respectively, and there wasno significant difference between T1 and T3. Compared with T1, the 12.5, 25.0, 37.5, and 50.0% substitution rates in T5, T4, T3, and T2 significantly reduced total nitrogen losses(NH_(3), N_(2)O) by 8.3, 16.1, 18.7, and 27.0%, respectively.Nitrogen use efficiency(NUE) was higher in T5, T3, and T1, and there were no significant differences among them.Organic fertilizer substitution directly reduced NH_(3)volatilization and N_(2)O emission from farmland by lowering theammonium nitrogen and alkali-dissolved N contents and by increasing soil moisture. These substitution treatmentsreduced N_(2)O emissions indirectly by regulating the abundances of AOB and nirK-harboring genes by promotingsoil moisture. Specifically, the 37.5% organic fertilizer substitution reduces NH_(3)volatilization and N_(2)O emission from farmland by reducing the ammonium nitrogen and alkali-dissolved N contents and increasing moisture, which negatively regulate the abundance of AOB and nir K-harboring genes to reduce N_(2)O emissions indirectly in rainfed maize fields on the Loess Plateau of China.
基金supported by the National Natural Science Foundation of China(No.32071980)the Key Projects of Shaanxi Agricultural Collaborative Innovation and Extension Alliance(No.LMZD202201)+1 种基金the Key R&D Project in Shaanxi Province(No.2021LLRH-07)Shaanxi Natural Scientific Basic Research Program project(No.2022JQ-157).
文摘Agricultural practices significantly contribute to greenhouse gas(GHG)emissions,necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production.Plastic film mulching is commonly used in the Loess Plateau region.Incorporating slow-release fertilizers as a replacement for urea within this practice can reduce nitrogen losses and enhance crop productivity.Combining these techniques represents a novel agricultural approach in semi-arid areas.However,the impact of this integration on soil carbon storage(SOCS),carbon footprint(CF),and economic benefits has received limited research attention.Therefore,we conducted an eight-year study(2015-2022)in the semi-arid northwestern region to quantify the effects of four treatments[urea supplied without plastic film mulching(CK-U),slow-release fertilizer supplied without plastic film mulching(CK-S),urea supplied with plastic film mulching(PM-U),and slow-release fertilizer supplied with plastic film mulching(PM-S)]on soil fertility,economic and environmental benefits.The results revealed that nitrogen fertilizer was the primary contributor to total GHG emissions(≥71.97%).Compared to other treatments,PM-S increased average grain yield by 12.01%-37.89%,water use efficiency by 9.19%-23.33%,nitrogen accumulation by 27.07%-66.19%,and net return by 6.21%-29.57%.Furthermore,PM-S decreased CF by 12.87%-44.31%and CF per net return by 14.25%-41.16%.After eight years,PM-S increased SOCS(0-40 cm)by 2.46%,while PM-U decreased it by 7.09%.These findings highlight the positive effects of PM-S on surface soil fertility,economic gains,and environmental benefits in spring maize production on the Loess Plateau,underscoring its potential for widespread adoption and application.
文摘目的通过系统评价和Meta分析方法,比较聚乙二醇化重组人粒细胞集落刺激因子(pegylated recombinant human granulocyte colony-stimulating factor,PEG-rhG-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)在预防恶性肿瘤化疗相关中性粒细胞减少症中的疗效与安全性差异,为临床合理用药提供循证医学依据。方法本研究采用系统性文献检索策略,全面检索Embase、Web of Science、PubMed、the Cochrane Library、中国知网、万方和维普数据库,收集从建库至2024年8月17日发表的关于PEG-rhG-CSF与rhG-CSF预防恶性肿瘤化疗相关中性粒细胞减少症的随机对照试验。由2名研究者独立进行文献筛选、数据提取及方法学质量评价,采用Cochrane偏倚风险评估工具对纳入研究进行质量评估。使用RevMan 5.4软件进行Meta分析,二分类变量采用风险比及其95%CI表示效应量,连续性变量采用均数差(MD)及其95%CI表示。研究间异质性采用I²统计量进行评估,I²>50%时采用随机效应模型,否则采用固定效应模型。结果共纳入28项随机对照临床试验,涉及2879例恶性肿瘤患者。根据Meta分析结果表明,PEG-rhG-CSF组在减少Ⅲ/Ⅳ度中性粒细胞减少持续时间方面优于rhG-CSF组(P<0.05)。不良反应事件发生率与rhG-CSF组差异没有统计学意义(P>0.05)。结论与rhG-CSF相比,PEG-rhG-CSF可显著缩短恶性肿瘤患者化疗后重度(Ⅲ/Ⅳ度)中性粒细胞减少的持续时间。基于现有循证医学证据,建议在恶性肿瘤化疗患者中优先选用PEG-rhG-CSF进行中性粒细胞减少症的预防,以降低感染风险,保障化疗周期顺利进行并改善患者预后。
基金supported by the National Natural Science Foundation of China(No.52170138)the National Key Research and Development Program of China(No.2023YFD1900305)+3 种基金Qingdao Agricultural University Doctoral Start-Up Fund(No.6631122003)the Project of Talent IntroductionEducation Program of Youth Innovation Teams in Universities of Shandong Province(2021–05)Shandong Provincial College Youth Innovation Team Program(No.2023KJ169).
文摘Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropriate handling of CFR not only waste valuable bioresources,but may also lead to the cyclosporin A and associated resistance genes into the natural environment,posing a significant threat to ecological system and human health.Land application was an effective way to resource recovery of CFR after aerobic composting(CAC).This study investigated the impact of CAC on soil fertility and environmental safety.The results indicated that CAC could improve soil nutrient contents and enhance enzyme activities.CAC altered the diversity and community composition of soil bacteria,resulting in an increase in the abundance of relevant bacteria beneficial for organic matter decomposition and cyclosporin A degradation.The introduced cyclosporin A(71.69μg/kg)completely degraded within 20 days due to soil biodegradation.The significantly increased abundance of intIl,mdr3,pgp,TSR and pmra in the soil cultivation early stage were restored to the soil background level within 90 days,indicating a reduced risk of antimicrobial resistance.The results demonstrated that reasonable land application of CAC could improve soil fertility without antimicrobial resistance risk,which is helpful for evaluating the resource utilization value and environmental risks of antibiotic fermentation residue after aerobic composting.
基金supported by the Shenyang Municipal Science and Technology Project,China(23-409-2-03)the Liaoning Provincial Department of Science and Technology Project,China(Z20230183)the Liaoning Provincial Applied Basic Research Program,China(2022JH2/101300173).
文摘Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil aggregates.However, the mechanism of these drivers in greenhouse soil fertilized with organic fertilizer is not well understood.In a 3-year field experiment, we aimed to investigate the factors which drive the stability of soil aggregates in greenhouse soil.To explore the impact of organic fertilizer on soil aggregates, we established four treatments:no fertilization (CK);inorganic fertilizer (CF);organic fertilizer (OF);and combined application of inorganic and organic fertilizers(COF).The application of organic fertilizer significantly enhanced the stability of aggregates, that is it enhanced the mean weight diameter, geometric mean diameter and aggregate content (%) of>0.25 mm aggregate fractions.OF and COF treatments increased the concentration of SOC, especially the aliphatic-C, aromatic-C and polysaccharide-C components of SOC, particularly in>0.25 mm aggregates.Organic fertilizer application significantly increased the content of free Fe(Fed), reactive Fe (Feo), and non-crystalline Fe in both bulk soil and aggregates.Furthermore, non-crystalline Fe showed a positive correlation with SOC content in both bulk soil and aggregates.Both non-crystalline Fe and SOC were significantly positively correlated with>2 mm mean weight diameter.Overall, we believe that the increase of SOC, aromatic-C, and non-crystal ine Fe concentrations in soil after the application of organic fertilizer is the reason for improving soil aggregate stability.
基金supported by Hebei Key Laboratory of Mineral Resources and Ecological Environment Monitoring(No.HBMREEM202302)Tianjin Key Research and Development Science and Technology Project(Nos.24YFXTHZ00170 and 24YFXTHZ00050)。
文摘The interaction mechanism between eggshell calcium and endogenous silica in biomass during biochar modification,and its impact on phosphate adsorption performance and slow-release fertilizer characteristics,remains unexplored.This study investigates that high silica content in biomass(>6%)inhibits the decomposition of CaCO_(3)in eggshells during pyrolysis,reducing the formation of active calcium species(CaO and Ca(OH)_(2)),while moderate silica levels(4%-5%)promote the formation of CaSiO_(3),enhancing phosphorus adsorption without hindering Ca^(2+)activation.Adsorption studies reveal that the precipitation of Ca_(5)(PO_(4))_(3)(OH)resulting from the combination of CaO and Ca(OH)_(2)with phosphate is the primary and effective form for phosphorus removal in calcium-modified adsorbents,accompanied by Ca_(3)(PO_(4))_(2)·2H_(2)O precipitation formed by CaSi O_(3).Eggshell calcium-modified corn straw biochar(ECS)exhibited the highest adsorption capacity,reaching 123.3 mg/g,outperforming materials in previous studies.ECS also demonstrated excellent pH adaptability and selective phosphate removal.As a biochar-based phosphorus fertilizer,ECS-P exhibits high phosphorus extractability in formic acid(93.92%)but low water solubility(0.62%),with phosphorus release during the seven-day intermittent leaching experiment remaining between 0.53 to 0.875 mg/L.These results confirm its potential as a phosphorus cycling fertilizer.This study provides fundamental insights into optimizing biomass selection based on silica content for calcium modification,offering an efficient strategy for both phosphate recovery and slow-release fertilizer development.
文摘Rice (Oryza sativa) is becoming a staplefood in many regions of DR-Congo. However, its production is still limited to the North Eastern part of the country and grain yield is low. A study was carried out in the city of Mbujimayi located in the Central part of the DR-Congo to assess the effects of organic and inorganic fertilizers on morpho-agronomic characteristics of O. sativa. The trial was conducted during the 2021 agricultural season A using a completely randomized design with three replicates. The six treatments studied consisted of application of T. diversifolia biomass at a dose of 2 kg/4m2 (BFT − 2 kg), 4 kg/4 m2 (BFT − 4 kg), inorganic fertilizer consisting with NPK17-17-17 + Urea (46% N) at a combined dose of 80 g/4 m2 (NP), 1/2 of the combination (BFT − 2 kg + NP) and finally 1/2 of the combination (BFT − 4 kg + NP). The untreated plots were used as controls. Plants treated with 1/2 combination (BFT − 4 kg + NP), BFT – 4 kg, and NP showed similar height (100.93 cm, 99.03 cm, and 98.63 cm, respectively) that were significantly higher than control and other treatments [1/2 (BFT – 2 kg + NP, BFT – 2 kg] For agronomic characteristics, days to 50% flowering varied between 73.00 and 74 days with an average of 74 days. The control and BFT – 4 kg showed significantly shorter panicles compared to other treatments. For yield components, 1/2 (BFT – 4 kg + NP) and the NP treatments generated a higher weight of 1000 grains. For yield per hectare, 1/2 (BFT − 4 kg + NP) induced significantly different levels of production than the control and other treatments, including 1/2 (BFT – 2 kg + NP), BFT – 4 kg + NP, BFT – 2 kg, BFT – 4 kg. The correlation coefficients between agronomic traits revealed that with the exception of the length of particle and the abortion rates, all the yield components (panicles per plant, seeds per panicle, weight of 1000 grains, and grail yield per plot) were strongly correlated with grain yield per hectare.
文摘Citrus is an important commercial crop in Uganda, especially the Eastern region. However, in spite of the increasing regional demand, citrus productivity is still low, attributed to pest and diseases, soil moisture stress, and low soil fertility, among others. Efforts to improve soil fertility are limited by inadequate supply of organic fertilizers due to competing demands. In addition, there is inadequate information on inorganic fertilizer requirements for citrus production in Uganda. The objective of this study was to develop optimum fertilizer recommendations for citrus production for Eastern Uganda. The study was conducted in Teso region, Eastern Uganda. Fertilizer (NPK, 17:17:17) was randomly applied to Hamlin, Valencia and Washington varieties with fertilizer and variety factorially arranged for each farm and citrus age range, replicated three times. Fertilizer rates were 0, 139, 278 and 556 kg NPK/ha for the 4 - 7-year old trees, and 0, 278, 556 and 1111 kg NPK/ha for the mature (8 years and above) trees. For a given variety, each fertilizer rate was applied onto three representative trees per farmer, six farmers per district. Results showed that yields and net profits were highest for variety Hamlin, and nearly the same for varieties Washington and Valencia. Fertilizer application increased fruit yield and profits for both the 4 to 7-year and 8 and above-year-old trees, with highest yield and profitability values observed at 556 kg NPK/ha. These results suggest applying 556 kg NPK/ha to citrus per year as an optimum fertilizer rate for citrus production in Teso region. The fertilizer should be applied in smaller splits of 800, 600, and 600 grams per tree, applied in April, June, and August.