Optical tweezers technology has the characteristics of noncontact manipulation in three dimensions and steerable separation in solutions,and could be applied to obtain a separated sperm with high quality for intracyto...Optical tweezers technology has the characteristics of noncontact manipulation in three dimensions and steerable separation in solutions,and could be applied to obtain a separated sperm with high quality for intracytoplasmic sperm injection(ICSI).However,the effects of optical tweezers on sperm motility are still unclear.To elucidate the effects on sperm motility for optical tweezers,we systematically investigated the correlation between motility parameters and the parameters of optical tweezers(wavelength,power,trapping duration,and trapping orientation).Under three systems of optical tweezers with different laser wavelengths(1064,850,and 785 nm),the nine motility parameters of free swimming were mainly affected by trapping orientation(vertical/horizontal)and trapping duration.When 850 nm laser and 1064 nm laser are used,vertical trapping significantly reduces sperm free-swimming capability with prolonged exposure time,whereas horizontal trapping exhibits relatively minor interference on sperm motility.Notably,the 785 nm laser does not induce statistically significant changes in key parameters of sperm motility under any experimental conditions(trapping orientation and duration).For the rolling frequency of trapped sperm,horizontal trapping for three wavelengths has a negligible effect compared with vertical trapping,especially for the 785 nm laser.In conclusion,horizontal trapping can preserve sperm motility under low power(below 140mW at 1064 nm,below 100mW at 850nm,below 60mW at 785 nm)and short duration(below 4 min).This trapping duration is suffcient for the separation procedure of single live sperm in ICSI.This study provides critical parameter optimization guidelines for the safe application of optical tweezers technology in reproductive medicine.展开更多
Objectives Neutrophil extracellular traps(NETs)have emerged as critical effectors in immune defense but also as potential drivers of tissue damage in chronic inflammatory diseases.Their role in periodontitis,a highly ...Objectives Neutrophil extracellular traps(NETs)have emerged as critical effectors in immune defense but also as potential drivers of tissue damage in chronic inflammatory diseases.Their role in periodontitis,a highly prevalent condition characterized by dysregulated host–microbe interactions,remains incompletely defined.This systematic review aimed to synthesize,for the first time,ex vivo human evidence on the presence,activity,and clinical significance of NETs in periodontitis.Methods A comprehensive search of Medline,Web of Science,and Scopus was conducted up to August 2025.Eligible studies included ex vivo human investigations assessing NETs or NET markers in gingival tissues,gingival crevicular fluid,saliva,blood,or biofilms from patients with periodontitis.Study selection,data extraction,and risk-of-bias assessment were conducted in duplicate,and the protocol was registered in PROSPERO(CRD420251109174).Results Seventeen studies met the inclusion criteria.NET markers such as citrullinated histone H3(CitH3),myeloperoxidase(MPO),and neutrophil elastase were consistently elevated in periodontitis samples compared with controls.Several studies reported a reduction in NET levels or improved NET degradation following periodontal therapy.NETs were also implicated in biofilm stability and in systemic associations with rheumatoid arthritis and chronic kidney disease.However,heterogeneity in methodologies,small sample sizes,and inconsistent marker use limited comparability across studies.Conclusions Ex vivo evidence indicates that aberrant NET formation and impaired clearance contribute to periodontal inflammation and tissue destruction.Nonetheless,methodological variability and risk of bias constrain definitive conclusions.Standardization of detection methods,consensus on marker panels,and exploration of neutrophil subsets and systemic confounders are essential to establish NETs as reliable biomarkers and therapeutic targets in periodontitis.展开更多
Owing to the presence of a low-energy,long-lived nuclear isomeric state,^(229)Th is an ideal candidate for developing the next generation clock—the nuclear clock—holding great promise for both applied and fundamenta...Owing to the presence of a low-energy,long-lived nuclear isomeric state,^(229)Th is an ideal candidate for developing the next generation clock—the nuclear clock—holding great promise for both applied and fundamental physics.The^(229)Th ionic nuclear optical clock has garnered considerable attention,attributed to its high precision with a relative uncertainty of≤1.5×10^(-19)and the potential for common-mode noise cancellation via self-comparison between the nuclear transition and the electronic transition of thorium ions.In this article,we focus on Th^(n+)ions(n=1,2,3)and present a comprehensive review of the current progress in the development of ionic nuclear clocks,covering essential steps such as ion generation,trapping,and cooling.Furthermore,we discuss the realization of a closed-loop clock cycle,addressing key aspects including stable isomer excitation and efficient isomer deexcitation.展开更多
Rare earth-doped inorganic compounds contribute mostly to the family of persistent luminescent materials due to the versatile energy levels of rare earth ions.One of the key research aims is to match the trap level st...Rare earth-doped inorganic compounds contribute mostly to the family of persistent luminescent materials due to the versatile energy levels of rare earth ions.One of the key research aims is to match the trap level stemming from the doped rare earth ion or intrinsic defects to the electronic structure of the host,and therefore thermoluminescence measurement becomes a radical technology in studying trap depth,which is one of the significant parameters that determine the properties of persistent luminescence and photostimulated luminescence.However,the results of trap depth obtained by different thermoluminescence methods are quite different so that they are not comparable.Herein,we analyzed different thermoluminescence methods,selected and improved the traditional peak position method of T_(m)/500 to be E=(-0.94Inβ+30.09)kT_(m).Only the experimental heating rate(β)is needed additionally,but the accuracy is improved greatly in most cases.This convenient and accurate method will accelerate the discovery of novel rare earth-doped materials.展开更多
In this study,we developed a single-beam optical trap-based surface-enhanced Raman scattering(SERS)optofluidic molecular fingerprint spectroscopy detection system.This system utilizes a single-beam optical trap to con...In this study,we developed a single-beam optical trap-based surface-enhanced Raman scattering(SERS)optofluidic molecular fingerprint spectroscopy detection system.This system utilizes a single-beam optical trap to concentrate free silver nanoparticles(AgNPs)within an optofluidic chip,significantly enhancing SERS performance.We investigated the optical field distribution characteristics within the tapered fiber using COMSOL simulation software and established a MATLAB simulation model to validate the single-beam optical trap's effectiveness in capturing AgNPs,demonstrating the theoretical feasibility of our approach.To verify the particle capture efficacy of the system,we experimentally controlled the optical trap's on-off state to manage the capture and release of particles precisely.The experimental results indicated that the Raman signal intensity in the capture state was significantly higher than in the non-capture state,confirming that the single-beam optical trap effectively enhances the SERS detection capability of the optofluidic detection system.Furthermore,we employed Raman mapping techniques to investigate the impact of the capture area on the SERS effect,revealing that the spectral intensity of molecular fingerprints in the laser-trapping region is significantly improved.We successfully detected the Raman spectrum of crystal violet at a concentration of 10^(−9)mol/L and pesticide thiram at a concentration of 10^(−5)mol/L,further demonstrating the ability of the single-beam optical trap in enhancing the molecular fingerprint spectrum identification capability of the SERS optofluidic chips.The optical trapping SERS optofluidic detection system developed in this study,as a key component of an integrated optoelectronic sensing system,holds the potential for integration with portable high-power lasers and high-performance Raman spectrometers.This integration is expected to advance highly integrated technologies and significantly enhance the overall performance and portability of optoelectronic sensing systems.展开更多
Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed t...Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.展开更多
The coherence time of an optically trapped neutral atom is a crucial parameter for quantum technologies.We found that optical dipole traps with higher-order spatial forms inherently offer lower decoherence rates compa...The coherence time of an optically trapped neutral atom is a crucial parameter for quantum technologies.We found that optical dipole traps with higher-order spatial forms inherently offer lower decoherence rates compared to those with lower-order spatial forms. We formulated the decoherence rate caused by the variance of the differential energy shift and photon jumping rate. Then, we constructed blue-detuned harmonic and quartic optical dipole traps, and experimentally investigated the coherence time of a trapped single cesium atom.Specifically, we demonstrated a significant improvement in the coherence time of a single atom by employing a quartic trap with the same characteristic trap potential and size as its harmonic counterpart—achieving an enhancement from 315 ms to 474 ms. The experimental results qualitatively verified our theory. Our approach provides a novel method to enhance the coherence time of optically trapped neutral atoms.展开更多
Beauty trap is a kind of traditional stratagem originating from ancient times.This stratagem is a type of strategic deception,making full use of human’s desires and weaknesses.No matter whether you are a genius or a ...Beauty trap is a kind of traditional stratagem originating from ancient times.This stratagem is a type of strategic deception,making full use of human’s desires and weaknesses.No matter whether you are a genius or a fool,you will have the opportunity to fall into a trap,especially if the trap is intended for you.Because of this characteristic,beauty trap was usually seen as a kind of effective non-military method,appearing in the war history of many different countries.With time going by,compared with its original form,this stratagem has greatly changed,developing many new forms.Some of them need to be adopted flexibly,combining with different kinds of psychological tactics.Except the field of military,this stratagem can also be seen in political struggles and commercial warfare.After coming to modern society,the beauty trap does not vanish.It seems that this stratagem will accompany the entire process of humanity,simply because the desires of humans will never disappear.展开更多
Natural products play a crucial role in new drug development,but their druggability is often limited by uncertain molecular targets and insufficient research on mechanisms of action.In this study,we developed a new RP...Natural products play a crucial role in new drug development,but their druggability is often limited by uncertain molecular targets and insufficient research on mechanisms of action.In this study,we developed a new RPL19-TRAP^(KI)-seq method,combining CRISPR/Cas9 and TRAP technologies,to investigate these mechanisms.We identified and validated seven ribosomal large subunit surface proteins suitable for TRAP,selecting RPL19 for its high enrichment.We successfully established a stable cell line expressing EGFP-RPL19 using CRISPR knock-in and verified its efficiency and specificity in enriching ribosomes and translating mRNA.Integrated with next-generation sequencing,this method allows precise detection of translating mRNA.We validated RPL19-TRAP^(KI)-seq by investigating rapamycin,an mTOR inhibitor,yielding results consistent with previous reports.This optimized TRAP technology provides an accurate representation of translating mRNA,closely reflecting protein expression levels.Furthermore,we investigated SBF-1,a 23-oxa-analog of natural saponin OSW-1 with significant anti-tumor activity but an unclear mechanism.Using RPL19-TRAP^(KI)-seq,we found that SBF-1 exerts its cytotoxic effects on tumor cells by disturbing cellular oxidative phosphorylation.In conclusion,our method has been proven to be a promising tool that can reveal the mechanisms of small molecules with greater accuracy,setting the stage for future exploration of small molecules and advancing the fields of pharmacology and therapeutic development.展开更多
We describe a three-dimensional(3D) magneto-optical trap(MOT) capable of simultaneously capturing ^(85)Rb and ^(133)Cs atoms. Unlike conventional setups, our system utilizes two separate laser systems that are combine...We describe a three-dimensional(3D) magneto-optical trap(MOT) capable of simultaneously capturing ^(85)Rb and ^(133)Cs atoms. Unlike conventional setups, our system utilizes two separate laser systems that are combined before entering the vacuum chamber, enabling the simultaneous trapping of two different atomic species. We trapped ^(85)Rb and ^(133)Cs atoms using relatively low total power: 8 mW cooling and 4 mW repump for ^(85)Rb, and 7.5 mW cooling and 1.5 mW repump for ^(133)Cs. The number of trapped atoms was 1.6 × 10^(8) for ^(85)Rb and 1.4 × 10^(8) for ^(133)Cs. The optical depths were 3.71 for ^(85)Rb and 3.45 for ^(133)Cs. The temperature of trapped atoms was ~200 μK for ^(85)Rb and ~200 μK for ^(133)Cs. Our 3D MOT setup allows full horizontal optical access to the trapped atomic ensembles without spatial interference from the trapping or repump laser beams. Our vacuum system is also quite simple, avoiding much of the complexity typically encountered in similar dual-species systems. However, the red detuning of the cooling laser used for atomic trapping in our system is relatively small, leaving room for further optimization. This system offers a versatile platform for exploring complex phenomena in ultracold atom physics, such as Rydberg molecule formation and interspecies interactions.展开更多
Recently,large-scale trapped ion systems have been realized in experiments for quantum simulation and quantum computation.They are the simplest systems for dynamical stability and parametric resonance.In this model,th...Recently,large-scale trapped ion systems have been realized in experiments for quantum simulation and quantum computation.They are the simplest systems for dynamical stability and parametric resonance.In this model,the Mathieu equation plays the most fundamental role for us to understand the stability and instability of a single ion.In this work,we investigate the dynamics of trapped ions with the Coulomb interaction based on the Hamiltonian equation.We show that the many-body interaction will not influence the phase diagram for instability.Then,the dynamics of this model in the large damping limit will also be analytically calculated using few trapped ions.Furthermore,we find that in the presence of modulation,synchronization dynamics can be observed,showing an exchange of velocities between distant ions on the left side and on the right side of the trap.These dynamics resemble that of the exchange of velocities in Newton's cradle for the collision of balls at the same time.These dynamics are independent of their initial conditions and the number of ions.As a unique feature of the interacting Mathieu equation,we hope this behavior,which leads to a quasi-periodic solution,can be measured in current experimental systems.Finally,we have also discussed the effect of anharmonic trapping potential,showing the desynchronization during the collision process.It is hoped that the dynamics in this many-body Mathieu equation with damping may find applications in quantum simulations.This model may also find interesting applications in dynamics systems as a pure mathematical problem,which may be beyond the results in the Floquet theorem.展开更多
Neutrophil extracellular traps(NET)have emerged as critical players in the pathogenesis of atherosclerosis and other cardiovascular diseases(CVD).These web-like structures,composed of DNA,histones,and granule proteins...Neutrophil extracellular traps(NET)have emerged as critical players in the pathogenesis of atherosclerosis and other cardiovascular diseases(CVD).These web-like structures,composed of DNA,histones,and granule proteins released by neutrophils,contribute significantly to both inflammation and thrombosis.This manuscript offers a comprehensive review of the recent literature on the involvement of NET in atherosclerosis,highlighting their interactions with various pathophysiological processes and their potential as biomarkers for CVD.Notably,the impact of radiation on NET formation is explored,emphasising how oxidative stress and inflammatory responses drive NET release,contributing to plaque instability.The role of histones,particularly citrullinated histones,in endothelial dysfunction and plaque progression is discussed,highlighting their significance in the pathophysiology of atherosclerosis.Furthermore,the complex relationship between lipoproteins and NET formation is examined,with a focus on how elevated low-density lipoprotein(LDL)and decreased high-density lipoprotein(HDL)levels facilitate NET release,thus promoting vascular inflammation and plaque instability.The influence of cholesterol on NET formation is also explored,underscoring its contribution to plaque development and stability.The role of Peptidylarginine deiminase 4(PAD4)in the regulation of NETosis is reviewed,with attention given to how PAD4-driven citrullination of histones affects atherosclerosis progression.Moreover,the manuscript examines the potential of NET components—such as double-stranded DNA,myeloperoxidase–DNA complexes,and citrullinated histone H3—as biomarkers for assessing disease severity and predicting adverse cardiovascular events,including ST-elevation myocardial infarction(STEMI)and stroke.Elevated levels of these biomarkers correlate with worse clinical outcomes,suggesting their utility in guiding therapeutic interventions.In contrast to the existing body of work,this review highlights the novelty of integrating recent findings on NET interactions with lipid metabolism,histone modifications,and PAD4 activity in the context of atherosclerosis.Overall,NET plays an integral role in the inflammatory and thrombotic processes underpinning atherosclerosis,and their components hold promise as both diagnostic markers and therapeutic targets in cardiovascular disease management.展开更多
Background:Liver metastases are a leading contributor to death among patients with colorectal cancer.Current clinical treatments,such as resection and systemic chemotherapy,are only applicable in a portion of cases.Mo...Background:Liver metastases are a leading contributor to death among patients with colorectal cancer.Current clinical treatments,such as resection and systemic chemotherapy,are only applicable in a portion of cases.More effective medical interventions,including those involving traditional Chinese medicine,could be beneficial for patients with newly diagnosed colorectal cancer to prevent the progression to liver metastasis.Xiaoyaosan(XYS)is a classical prescription in traditional Chinese medicine with a history of hundreds of years.Despite its well-known protective effects against breast cancer,the understanding of its application in colorectal cancer metastases remains limited.The anti-metastasis mechanism of XYS remains to be elucidated.In this research,we explored the impact of XYS against liver metastases of colorectal cancer and its potential mechanisms.Methods:Thirty-six SPF male C57BL/6 mice were randomly assigned to six groups:a control group,a model group,a DNase I group,and three XYS treatment groups receiving high,medium,and low doses,respectively.A mouse model for colorectal cancer liver metastasis was established through the splenic injection of MC38 cells.Twenty-one days after the injection of cancer cells,the number of metastatic foci and the weights of the liver were calculated,and HE staining was performed to evaluate the effect of XYS.Neutrophil extracellular traps(NETs)formation in the liver was detected by immunofluorescence staining,and NETs formation in the serum was detected by ELISA.The levels of CXCL1,CXCL2,G-CSF,and HMGB1 were determined using ELISA kits.The expression levels of the proteins p-p38,p38,p-ERK,and ERK were assessed using Western blot analysis.Results:XYS treatment reduced the number of metastatic foci,the weights of metastatic livers,and the infiltration area of tumor-like cells.XYS could inhibit NETs formation in the liver and serum of mice with metastasis.The concentrations of CXCL1,CXCL2,G-CSF,and HMGB1 were significantly decreased in all XYS-treated groups.Moreover,XYS down-regulated the protein expression levels of phosphorylated p38 and ERK.Conclusion:XYS could attenuate liver metastases of colorectal cancer in vivo.The inhibitory mechanism of XYS may involve the reduction of NETs formation through the regulation of tumor-derived factors and the downstream MAPKs(p38,ERK)signaling pathway.展开更多
基金supported by the Natural Science Foundation of Anhui Province in China(2508085MF166)Research Fund of Anhui Institute of Translational Medicine(2024zh-03)+2 种基金Key Scientific Research Foundation of Education Department of Anhui Province(2023AH040083)National Natural Science Foundation of China(12404353)Cultivation Project of Training Young and Middle-aged Teachers in Universities of Anhui Province(DTR2023013).
文摘Optical tweezers technology has the characteristics of noncontact manipulation in three dimensions and steerable separation in solutions,and could be applied to obtain a separated sperm with high quality for intracytoplasmic sperm injection(ICSI).However,the effects of optical tweezers on sperm motility are still unclear.To elucidate the effects on sperm motility for optical tweezers,we systematically investigated the correlation between motility parameters and the parameters of optical tweezers(wavelength,power,trapping duration,and trapping orientation).Under three systems of optical tweezers with different laser wavelengths(1064,850,and 785 nm),the nine motility parameters of free swimming were mainly affected by trapping orientation(vertical/horizontal)and trapping duration.When 850 nm laser and 1064 nm laser are used,vertical trapping significantly reduces sperm free-swimming capability with prolonged exposure time,whereas horizontal trapping exhibits relatively minor interference on sperm motility.Notably,the 785 nm laser does not induce statistically significant changes in key parameters of sperm motility under any experimental conditions(trapping orientation and duration).For the rolling frequency of trapped sperm,horizontal trapping for three wavelengths has a negligible effect compared with vertical trapping,especially for the 785 nm laser.In conclusion,horizontal trapping can preserve sperm motility under low power(below 140mW at 1064 nm,below 100mW at 850nm,below 60mW at 785 nm)and short duration(below 4 min).This trapping duration is suffcient for the separation procedure of single live sperm in ICSI.This study provides critical parameter optimization guidelines for the safe application of optical tweezers technology in reproductive medicine.
文摘Objectives Neutrophil extracellular traps(NETs)have emerged as critical effectors in immune defense but also as potential drivers of tissue damage in chronic inflammatory diseases.Their role in periodontitis,a highly prevalent condition characterized by dysregulated host–microbe interactions,remains incompletely defined.This systematic review aimed to synthesize,for the first time,ex vivo human evidence on the presence,activity,and clinical significance of NETs in periodontitis.Methods A comprehensive search of Medline,Web of Science,and Scopus was conducted up to August 2025.Eligible studies included ex vivo human investigations assessing NETs or NET markers in gingival tissues,gingival crevicular fluid,saliva,blood,or biofilms from patients with periodontitis.Study selection,data extraction,and risk-of-bias assessment were conducted in duplicate,and the protocol was registered in PROSPERO(CRD420251109174).Results Seventeen studies met the inclusion criteria.NET markers such as citrullinated histone H3(CitH3),myeloperoxidase(MPO),and neutrophil elastase were consistently elevated in periodontitis samples compared with controls.Several studies reported a reduction in NET levels or improved NET degradation following periodontal therapy.NETs were also implicated in biofilm stability and in systemic associations with rheumatoid arthritis and chronic kidney disease.However,heterogeneity in methodologies,small sample sizes,and inconsistent marker use limited comparability across studies.Conclusions Ex vivo evidence indicates that aberrant NET formation and impaired clearance contribute to periodontal inflammation and tissue destruction.Nonetheless,methodological variability and risk of bias constrain definitive conclusions.Standardization of detection methods,consensus on marker panels,and exploration of neutrophil subsets and systemic confounders are essential to establish NETs as reliable biomarkers and therapeutic targets in periodontitis.
基金Project supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB0920000)the National Natural Science Foundation of China(Grant No.12341401)。
文摘Owing to the presence of a low-energy,long-lived nuclear isomeric state,^(229)Th is an ideal candidate for developing the next generation clock—the nuclear clock—holding great promise for both applied and fundamental physics.The^(229)Th ionic nuclear optical clock has garnered considerable attention,attributed to its high precision with a relative uncertainty of≤1.5×10^(-19)and the potential for common-mode noise cancellation via self-comparison between the nuclear transition and the electronic transition of thorium ions.In this article,we focus on Th^(n+)ions(n=1,2,3)and present a comprehensive review of the current progress in the development of ionic nuclear clocks,covering essential steps such as ion generation,trapping,and cooling.Furthermore,we discuss the realization of a closed-loop clock cycle,addressing key aspects including stable isomer excitation and efficient isomer deexcitation.
基金Project supported by the National Natural Science Foundation of China(52372134,12274023)the Fundamental Re search Funds for the Central Universities(FRF-EYIT-23-04)。
文摘Rare earth-doped inorganic compounds contribute mostly to the family of persistent luminescent materials due to the versatile energy levels of rare earth ions.One of the key research aims is to match the trap level stemming from the doped rare earth ion or intrinsic defects to the electronic structure of the host,and therefore thermoluminescence measurement becomes a radical technology in studying trap depth,which is one of the significant parameters that determine the properties of persistent luminescence and photostimulated luminescence.However,the results of trap depth obtained by different thermoluminescence methods are quite different so that they are not comparable.Herein,we analyzed different thermoluminescence methods,selected and improved the traditional peak position method of T_(m)/500 to be E=(-0.94Inβ+30.09)kT_(m).Only the experimental heating rate(β)is needed additionally,but the accuracy is improved greatly in most cases.This convenient and accurate method will accelerate the discovery of novel rare earth-doped materials.
基金financial supports from National Natural Science Foundation of China(62175023).
文摘In this study,we developed a single-beam optical trap-based surface-enhanced Raman scattering(SERS)optofluidic molecular fingerprint spectroscopy detection system.This system utilizes a single-beam optical trap to concentrate free silver nanoparticles(AgNPs)within an optofluidic chip,significantly enhancing SERS performance.We investigated the optical field distribution characteristics within the tapered fiber using COMSOL simulation software and established a MATLAB simulation model to validate the single-beam optical trap's effectiveness in capturing AgNPs,demonstrating the theoretical feasibility of our approach.To verify the particle capture efficacy of the system,we experimentally controlled the optical trap's on-off state to manage the capture and release of particles precisely.The experimental results indicated that the Raman signal intensity in the capture state was significantly higher than in the non-capture state,confirming that the single-beam optical trap effectively enhances the SERS detection capability of the optofluidic detection system.Furthermore,we employed Raman mapping techniques to investigate the impact of the capture area on the SERS effect,revealing that the spectral intensity of molecular fingerprints in the laser-trapping region is significantly improved.We successfully detected the Raman spectrum of crystal violet at a concentration of 10^(−9)mol/L and pesticide thiram at a concentration of 10^(−5)mol/L,further demonstrating the ability of the single-beam optical trap in enhancing the molecular fingerprint spectrum identification capability of the SERS optofluidic chips.The optical trapping SERS optofluidic detection system developed in this study,as a key component of an integrated optoelectronic sensing system,holds the potential for integration with portable high-power lasers and high-performance Raman spectrometers.This integration is expected to advance highly integrated technologies and significantly enhance the overall performance and portability of optoelectronic sensing systems.
基金the support of the National Natural Science Foundation of China (Grant No.82272503)Natural Science Foundation of Zhejiang Province (Grant No. LQN25H060006)
文摘Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.
基金supported by the National Key Research and Development Program of China (Grant No.2021YFA1402002)the Innovation Program for Quantum Science and Technology (Grant No.2023ZD0300400)the National Natural Science Foundation of China (Grant Nos.U21A6006,U21A20433,92465201,12474360,and 92265108)。
文摘The coherence time of an optically trapped neutral atom is a crucial parameter for quantum technologies.We found that optical dipole traps with higher-order spatial forms inherently offer lower decoherence rates compared to those with lower-order spatial forms. We formulated the decoherence rate caused by the variance of the differential energy shift and photon jumping rate. Then, we constructed blue-detuned harmonic and quartic optical dipole traps, and experimentally investigated the coherence time of a trapped single cesium atom.Specifically, we demonstrated a significant improvement in the coherence time of a single atom by employing a quartic trap with the same characteristic trap potential and size as its harmonic counterpart—achieving an enhancement from 315 ms to 474 ms. The experimental results qualitatively verified our theory. Our approach provides a novel method to enhance the coherence time of optically trapped neutral atoms.
文摘Beauty trap is a kind of traditional stratagem originating from ancient times.This stratagem is a type of strategic deception,making full use of human’s desires and weaknesses.No matter whether you are a genius or a fool,you will have the opportunity to fall into a trap,especially if the trap is intended for you.Because of this characteristic,beauty trap was usually seen as a kind of effective non-military method,appearing in the war history of many different countries.With time going by,compared with its original form,this stratagem has greatly changed,developing many new forms.Some of them need to be adopted flexibly,combining with different kinds of psychological tactics.Except the field of military,this stratagem can also be seen in political struggles and commercial warfare.After coming to modern society,the beauty trap does not vanish.It seems that this stratagem will accompany the entire process of humanity,simply because the desires of humans will never disappear.
基金supported by the National Key Research and Development Program of China(No.2022YFC2804800 to W.J.)the National Natural Science Foundation of China(No.22494704.,22137002 to Y.D.,92253305 to W.J.and 31971111 to C.L.)+6 种基金the Science and Technology Commission of Shanghai Municipality(Grant 20JC1410900 to Y.D.)the University Innovation Research Group in Chongqing(No.CXQT21016 to Y.D.)the Chongqing Talent Program Project(No.CQYC20200302119 to Y.D.)High-Level Innovation Platform Cultivation Plan of Chongqing(to Y.D.)Joint Fund of the Natural Science Innovation and Development Foundation of Chongqing(to Y.D.)Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning(to W.J.)Chongqing Doctoral Express Entry Project(No.CSTB2022BSXM-JCX0044 to J.H.).
文摘Natural products play a crucial role in new drug development,but their druggability is often limited by uncertain molecular targets and insufficient research on mechanisms of action.In this study,we developed a new RPL19-TRAP^(KI)-seq method,combining CRISPR/Cas9 and TRAP technologies,to investigate these mechanisms.We identified and validated seven ribosomal large subunit surface proteins suitable for TRAP,selecting RPL19 for its high enrichment.We successfully established a stable cell line expressing EGFP-RPL19 using CRISPR knock-in and verified its efficiency and specificity in enriching ribosomes and translating mRNA.Integrated with next-generation sequencing,this method allows precise detection of translating mRNA.We validated RPL19-TRAP^(KI)-seq by investigating rapamycin,an mTOR inhibitor,yielding results consistent with previous reports.This optimized TRAP technology provides an accurate representation of translating mRNA,closely reflecting protein expression levels.Furthermore,we investigated SBF-1,a 23-oxa-analog of natural saponin OSW-1 with significant anti-tumor activity but an unclear mechanism.Using RPL19-TRAP^(KI)-seq,we found that SBF-1 exerts its cytotoxic effects on tumor cells by disturbing cellular oxidative phosphorylation.In conclusion,our method has been proven to be a promising tool that can reveal the mechanisms of small molecules with greater accuracy,setting the stage for future exploration of small molecules and advancing the fields of pharmacology and therapeutic development.
基金Project supported by the National Key R&D Program of China (Grant No. 2022YFA1404002)the National Natural Science Foundation of China (Grant Nos. U20A20218, 61525504, 61435011, and T2495253)+1 种基金the Anhui Initiative in Quantum Information Technologies (Grant No. AHY020200)the Major Science and Technology Projects in Anhui Province (Grant No. 202203a13010001)。
文摘We describe a three-dimensional(3D) magneto-optical trap(MOT) capable of simultaneously capturing ^(85)Rb and ^(133)Cs atoms. Unlike conventional setups, our system utilizes two separate laser systems that are combined before entering the vacuum chamber, enabling the simultaneous trapping of two different atomic species. We trapped ^(85)Rb and ^(133)Cs atoms using relatively low total power: 8 mW cooling and 4 mW repump for ^(85)Rb, and 7.5 mW cooling and 1.5 mW repump for ^(133)Cs. The number of trapped atoms was 1.6 × 10^(8) for ^(85)Rb and 1.4 × 10^(8) for ^(133)Cs. The optical depths were 3.71 for ^(85)Rb and 3.45 for ^(133)Cs. The temperature of trapped atoms was ~200 μK for ^(85)Rb and ~200 μK for ^(133)Cs. Our 3D MOT setup allows full horizontal optical access to the trapped atomic ensembles without spatial interference from the trapping or repump laser beams. Our vacuum system is also quite simple, avoiding much of the complexity typically encountered in similar dual-species systems. However, the red detuning of the cooling laser used for atomic trapping in our system is relatively small, leaving room for further optimization. This system offers a versatile platform for exploring complex phenomena in ultracold atom physics, such as Rydberg molecule formation and interspecies interactions.
基金supported by the Innovation Program for Quantum Science and Technology(Grant Nos.2021ZD0301200,2021ZD0303200,and 2021ZD0301500)the Alliance of International Science Organizations(ANSO)。
文摘Recently,large-scale trapped ion systems have been realized in experiments for quantum simulation and quantum computation.They are the simplest systems for dynamical stability and parametric resonance.In this model,the Mathieu equation plays the most fundamental role for us to understand the stability and instability of a single ion.In this work,we investigate the dynamics of trapped ions with the Coulomb interaction based on the Hamiltonian equation.We show that the many-body interaction will not influence the phase diagram for instability.Then,the dynamics of this model in the large damping limit will also be analytically calculated using few trapped ions.Furthermore,we find that in the presence of modulation,synchronization dynamics can be observed,showing an exchange of velocities between distant ions on the left side and on the right side of the trap.These dynamics resemble that of the exchange of velocities in Newton's cradle for the collision of balls at the same time.These dynamics are independent of their initial conditions and the number of ions.As a unique feature of the interacting Mathieu equation,we hope this behavior,which leads to a quasi-periodic solution,can be measured in current experimental systems.Finally,we have also discussed the effect of anharmonic trapping potential,showing the desynchronization during the collision process.It is hoped that the dynamics in this many-body Mathieu equation with damping may find applications in quantum simulations.This model may also find interesting applications in dynamics systems as a pure mathematical problem,which may be beyond the results in the Floquet theorem.
基金supported by NIH grants to MI Bukrinsky(R01NS124477 and P30AI117970)by the“Creation of Experimental Laboratories in the Natural Sciences Program”and Basic Research Programat Higher School of Economics University.
文摘Neutrophil extracellular traps(NET)have emerged as critical players in the pathogenesis of atherosclerosis and other cardiovascular diseases(CVD).These web-like structures,composed of DNA,histones,and granule proteins released by neutrophils,contribute significantly to both inflammation and thrombosis.This manuscript offers a comprehensive review of the recent literature on the involvement of NET in atherosclerosis,highlighting their interactions with various pathophysiological processes and their potential as biomarkers for CVD.Notably,the impact of radiation on NET formation is explored,emphasising how oxidative stress and inflammatory responses drive NET release,contributing to plaque instability.The role of histones,particularly citrullinated histones,in endothelial dysfunction and plaque progression is discussed,highlighting their significance in the pathophysiology of atherosclerosis.Furthermore,the complex relationship between lipoproteins and NET formation is examined,with a focus on how elevated low-density lipoprotein(LDL)and decreased high-density lipoprotein(HDL)levels facilitate NET release,thus promoting vascular inflammation and plaque instability.The influence of cholesterol on NET formation is also explored,underscoring its contribution to plaque development and stability.The role of Peptidylarginine deiminase 4(PAD4)in the regulation of NETosis is reviewed,with attention given to how PAD4-driven citrullination of histones affects atherosclerosis progression.Moreover,the manuscript examines the potential of NET components—such as double-stranded DNA,myeloperoxidase–DNA complexes,and citrullinated histone H3—as biomarkers for assessing disease severity and predicting adverse cardiovascular events,including ST-elevation myocardial infarction(STEMI)and stroke.Elevated levels of these biomarkers correlate with worse clinical outcomes,suggesting their utility in guiding therapeutic interventions.In contrast to the existing body of work,this review highlights the novelty of integrating recent findings on NET interactions with lipid metabolism,histone modifications,and PAD4 activity in the context of atherosclerosis.Overall,NET plays an integral role in the inflammatory and thrombotic processes underpinning atherosclerosis,and their components hold promise as both diagnostic markers and therapeutic targets in cardiovascular disease management.
基金supported by Sichuan Science and Technology Program(grant number 2023NSFSC1809)Hospital of Chengdu University of Traditional Chinese Medicine(grant number 23ZYTS1004,21YY01).
文摘Background:Liver metastases are a leading contributor to death among patients with colorectal cancer.Current clinical treatments,such as resection and systemic chemotherapy,are only applicable in a portion of cases.More effective medical interventions,including those involving traditional Chinese medicine,could be beneficial for patients with newly diagnosed colorectal cancer to prevent the progression to liver metastasis.Xiaoyaosan(XYS)is a classical prescription in traditional Chinese medicine with a history of hundreds of years.Despite its well-known protective effects against breast cancer,the understanding of its application in colorectal cancer metastases remains limited.The anti-metastasis mechanism of XYS remains to be elucidated.In this research,we explored the impact of XYS against liver metastases of colorectal cancer and its potential mechanisms.Methods:Thirty-six SPF male C57BL/6 mice were randomly assigned to six groups:a control group,a model group,a DNase I group,and three XYS treatment groups receiving high,medium,and low doses,respectively.A mouse model for colorectal cancer liver metastasis was established through the splenic injection of MC38 cells.Twenty-one days after the injection of cancer cells,the number of metastatic foci and the weights of the liver were calculated,and HE staining was performed to evaluate the effect of XYS.Neutrophil extracellular traps(NETs)formation in the liver was detected by immunofluorescence staining,and NETs formation in the serum was detected by ELISA.The levels of CXCL1,CXCL2,G-CSF,and HMGB1 were determined using ELISA kits.The expression levels of the proteins p-p38,p38,p-ERK,and ERK were assessed using Western blot analysis.Results:XYS treatment reduced the number of metastatic foci,the weights of metastatic livers,and the infiltration area of tumor-like cells.XYS could inhibit NETs formation in the liver and serum of mice with metastasis.The concentrations of CXCL1,CXCL2,G-CSF,and HMGB1 were significantly decreased in all XYS-treated groups.Moreover,XYS down-regulated the protein expression levels of phosphorylated p38 and ERK.Conclusion:XYS could attenuate liver metastases of colorectal cancer in vivo.The inhibitory mechanism of XYS may involve the reduction of NETs formation through the regulation of tumor-derived factors and the downstream MAPKs(p38,ERK)signaling pathway.
