In modern pig breeding programs,growth and fatness are vital economic traits that significantly influence porcine production.To identify underlying variants and candidate genes associated with growth and fatness trait...In modern pig breeding programs,growth and fatness are vital economic traits that significantly influence porcine production.To identify underlying variants and candidate genes associated with growth and fatness traits,a total of 1067 genotyped Duroc pigs with de-regressed estimated breeding values(DEBV)records were analyzed in a genome wide association study(GWAS)by using a single marker regression model.In total,28 potential single nucleotide polymorphisms(SNPs)were associated with these traits of interest.Moreover,VPS4 B,PHLPP1,and some other genes were highlighted as functionally plausible candidate genes that compose the underlying genetic architecture of porcine growth and fatness traits.Our findings contribute to a better understanding of the genetic architectures underlying swine growth and fatness traits that can be potentially used in pig breeding programs.展开更多
Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) c...Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.展开更多
脂肪酸转运蛋白1(Fatty acid transport protein 1,FATP1)是脂肪酸转运蛋白家族(FATPs)的一员,具有促进脂肪细胞吸收利用长链和极长链脂肪酸的功能,在脂肪沉积过程中发挥着重要的作用。采用直接测序法寻找FATP1基因5'调控区+64～-72...脂肪酸转运蛋白1(Fatty acid transport protein 1,FATP1)是脂肪酸转运蛋白家族(FATPs)的一员,具有促进脂肪细胞吸收利用长链和极长链脂肪酸的功能,在脂肪沉积过程中发挥着重要的作用。采用直接测序法寻找FATP1基因5'调控区+64～-723 bp片段的单核苷酸多态性,在-586 bp处发现了一个T→C突变SNP(单核苷酸多态性)座位,利用PCR-RFLP的方法检测它在3个试验猪群体中的基因型,发现该座位在皮特兰和金皮F2代猪群中存在多态性,而在金华猪中仅表现为FATP1g-586TFATP1g-586T基因型。建立混合模型方程组分析该座位对188头金皮F2代猪脂肪性状的效应,结果显示,猪FATP1基因第-586座位的不同基因型与肩背膘厚、6～7肋背膘厚、最后一根肋骨处背膘厚、平均背膘厚和板油重显著相关(P<0.05),表明FATP1基因5'调控区的第-586位上的T→C突变对猪脂肪沉积有一定的影响。展开更多
In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were ide...In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C1 allele were 0.79–0.85, and frequencies of C2 allele were 0.21–0.15. At g.6852C>T locus, frequencies of C3 allele were 0.70–0.72, and frequencies of T3 allele were 0.30–0.28. Compared with goats with C1C1 and C3C3, those with C1C2 and C3T3 genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C1C1C3T3 and C1C2C3T3 had higher milk yield than those with C1C2C3C3 (P < 0.05). In addition, the combined effect of C1C2C3C3 on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).展开更多
【目的】高脂饮食(high-fat diet)已被证明可诱发高脂血症(hyperlipidemia)等多种疾病,对人体健康造成威胁。本研究通过在桑叶中添加大豆油,调查高脂饮食对家蚕Bombyx mori生长发育、经济性状、脂质代谢和免疫功能的影响,评估以家蚕构...【目的】高脂饮食(high-fat diet)已被证明可诱发高脂血症(hyperlipidemia)等多种疾病,对人体健康造成威胁。本研究通过在桑叶中添加大豆油,调查高脂饮食对家蚕Bombyx mori生长发育、经济性状、脂质代谢和免疫功能的影响,评估以家蚕构建高脂血症疾病模型为人类健康研究提供服务的可行性。【方法】选取4龄起蚕和5龄起蚕分别进行高脂饮食(分别用0.5%,1.0%和2.0%大豆油处理的桑叶)饲养,直至熟蚕上簇,测量各日龄幼虫体重和蚕茧茧层率。通过甘油三酯(triglyceride)测试盒检测用0.5%大豆油处理的桑叶饲喂的5龄幼虫脂肪体和血清中甘油三酯含量;采用油红O染色(oil red O staining)观察脂滴的形态变化和平均光密度值;利用实时荧光定量PCR分析5龄幼虫脂肪体中8个免疫相关基因(BmCecD2,BmCecA,BmCecB,BmCecE,BmCec-CBM2-2,BmSpz1,BmDual和BmKayak)的转录水平。【结果】与对照组(普通桑叶饲养)相比,高脂饮食(分别用0.5%,1.0%和2.0%大豆油处理的桑叶)显著抑制了家蚕幼虫的体重增长,使蚕茧茧层率显著降低。与对照组相比,高脂饮食(0.5%大豆油处理的桑叶)使家蚕5龄幼虫脂肪体和血清中的甘油三酯含量显著升高;脂肪体形态和脂滴累积异常;5龄幼虫脂肪体中检测的8个先天免疫相关基因的表达量显著下调,其中BmCecE,BmDual和BmKayak的表达量下调超过90%。【结论】高脂饮食导致家蚕生长缓慢、发育延迟,经济性状下降,脂质代谢紊乱以及免疫调节功能减弱。其中,甘油三酯含量的增加与人类高脂血症疾病生理病理特征相似,本研究为利用昆虫构建疾病模型提供了理论依据。展开更多
基金the earmarked fund for China Agriculture Research System(CARS-35)the National Natural Science Foundation of China(31772556)the Key R&D Program of Guangdong Province,China(2018B020203002)。
文摘In modern pig breeding programs,growth and fatness are vital economic traits that significantly influence porcine production.To identify underlying variants and candidate genes associated with growth and fatness traits,a total of 1067 genotyped Duroc pigs with de-regressed estimated breeding values(DEBV)records were analyzed in a genome wide association study(GWAS)by using a single marker regression model.In total,28 potential single nucleotide polymorphisms(SNPs)were associated with these traits of interest.Moreover,VPS4 B,PHLPP1,and some other genes were highlighted as functionally plausible candidate genes that compose the underlying genetic architecture of porcine growth and fatness traits.Our findings contribute to a better understanding of the genetic architectures underlying swine growth and fatness traits that can be potentially used in pig breeding programs.
文摘Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.
文摘脂肪酸转运蛋白1(Fatty acid transport protein 1,FATP1)是脂肪酸转运蛋白家族(FATPs)的一员,具有促进脂肪细胞吸收利用长链和极长链脂肪酸的功能,在脂肪沉积过程中发挥着重要的作用。采用直接测序法寻找FATP1基因5'调控区+64～-723 bp片段的单核苷酸多态性,在-586 bp处发现了一个T→C突变SNP(单核苷酸多态性)座位,利用PCR-RFLP的方法检测它在3个试验猪群体中的基因型,发现该座位在皮特兰和金皮F2代猪群中存在多态性,而在金华猪中仅表现为FATP1g-586TFATP1g-586T基因型。建立混合模型方程组分析该座位对188头金皮F2代猪脂肪性状的效应,结果显示,猪FATP1基因第-586座位的不同基因型与肩背膘厚、6～7肋背膘厚、最后一根肋骨处背膘厚、平均背膘厚和板油重显著相关(P<0.05),表明FATP1基因5'调控区的第-586位上的T→C突变对猪脂肪沉积有一定的影响。
文摘In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C1 allele were 0.79–0.85, and frequencies of C2 allele were 0.21–0.15. At g.6852C>T locus, frequencies of C3 allele were 0.70–0.72, and frequencies of T3 allele were 0.30–0.28. Compared with goats with C1C1 and C3C3, those with C1C2 and C3T3 genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C1C1C3T3 and C1C2C3T3 had higher milk yield than those with C1C2C3C3 (P < 0.05). In addition, the combined effect of C1C2C3C3 on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).
文摘【目的】高脂饮食(high-fat diet)已被证明可诱发高脂血症(hyperlipidemia)等多种疾病,对人体健康造成威胁。本研究通过在桑叶中添加大豆油,调查高脂饮食对家蚕Bombyx mori生长发育、经济性状、脂质代谢和免疫功能的影响,评估以家蚕构建高脂血症疾病模型为人类健康研究提供服务的可行性。【方法】选取4龄起蚕和5龄起蚕分别进行高脂饮食(分别用0.5%,1.0%和2.0%大豆油处理的桑叶)饲养,直至熟蚕上簇,测量各日龄幼虫体重和蚕茧茧层率。通过甘油三酯(triglyceride)测试盒检测用0.5%大豆油处理的桑叶饲喂的5龄幼虫脂肪体和血清中甘油三酯含量;采用油红O染色(oil red O staining)观察脂滴的形态变化和平均光密度值;利用实时荧光定量PCR分析5龄幼虫脂肪体中8个免疫相关基因(BmCecD2,BmCecA,BmCecB,BmCecE,BmCec-CBM2-2,BmSpz1,BmDual和BmKayak)的转录水平。【结果】与对照组(普通桑叶饲养)相比,高脂饮食(分别用0.5%,1.0%和2.0%大豆油处理的桑叶)显著抑制了家蚕幼虫的体重增长,使蚕茧茧层率显著降低。与对照组相比,高脂饮食(0.5%大豆油处理的桑叶)使家蚕5龄幼虫脂肪体和血清中的甘油三酯含量显著升高;脂肪体形态和脂滴累积异常;5龄幼虫脂肪体中检测的8个先天免疫相关基因的表达量显著下调,其中BmCecE,BmDual和BmKayak的表达量下调超过90%。【结论】高脂饮食导致家蚕生长缓慢、发育延迟,经济性状下降,脂质代谢紊乱以及免疫调节功能减弱。其中,甘油三酯含量的增加与人类高脂血症疾病生理病理特征相似,本研究为利用昆虫构建疾病模型提供了理论依据。
