Since China began its reform and opening-up policy in the late 1970s,its economic growth has continued at an annual rate of nearly 10%.Such rapid growth derives from a few fast-growing sectors.This paper examines thei...Since China began its reform and opening-up policy in the late 1970s,its economic growth has continued at an annual rate of nearly 10%.Such rapid growth derives from a few fast-growing sectors.This paper examines their growth,both overall and by sector,as well as their distribution,nature,growth momentum and impact on industrial growth and restructuring.By means of international comparative research,this paper concludes that manufacturing growth in industrialized countries is not necessarily outpaced by services.In some time periods,manufacturing tends to grow much faster than the service industry.展开更多
The aim of the study was to assess the effect of natural phytoremediation, impact of growing fast-growing trees on contaminated agricultural land on ecosystem services using a Matrix evaluation system. The Matrix syst...The aim of the study was to assess the effect of natural phytoremediation, impact of growing fast-growing trees on contaminated agricultural land on ecosystem services using a Matrix evaluation system. The Matrix system is a table that links an ecosystem to its potential to provide ecosystem services. To determine the monetary price of natural capital for the provision of ecosystem services, we chose the value transfer method, based on which it is possible to assign a price (1 point = 40.7 EUR) to point values. Monitoring of the study site Kuchyňa (Mollic Fluvisol) is running since year 2010. The fast-growing willow was planted (between 2006 to 2018 year) on an area of about 43 hectares. During the monitoring of soil parameters, we observed a slight decrease in the value of the active soil reaction, a negative trend in the content of available phosphorus and a positive trend in the development of the total content risk elements in the soil. The phytoremediation ability of willow regarding risk elements was manifested by an increase in the value and price of production and regulatory ecosystem services at the monitored location. The price of natural phytoremediation of fast-growing willow on the given area was 218762.5 EUR for production services and 171509.8 EUR for regulatory services.展开更多
Background Body weight is an important indicator of the overall health and production efficiency in broiler chickens.In broiler houses,body weight of chicks is variable despite the same genetics,hatching and feeding p...Background Body weight is an important indicator of the overall health and production efficiency in broiler chickens.In broiler houses,body weight of chicks is variable despite the same genetics,hatching and feeding practices within a production system.The objective of this study was to investigate the intestinal microbiota and bile salt hydrolase(BSH)activity in slow and fast growing broiler chickens,which belonged to the 10th and 90th percentile body weight groups,respectively.Methods A total of 300 Ross 308 broiler chickens(100 per cohort from three independent cohorts)were selected and mucosal samples from the jejunum,ileum,and cecum were collected at day of arrival,11 and 25(n=450).Then,bacterial counts,16S rRNA amplicon sequencing,species specific real-time qPCR,as well as BSH activity were analyzed.Results Results of bacterial counts showed no significant difference between slow and fast growing cohorts(P>0.05),but they tended to be higher in the slow growing chickens in all measured bacterial groups in cecum.The 16S rRNA amplicon sequencing revealed higher relative abundance of E.coli-Shigella(71.3%−79.8%)at day of arrival,while the most abundant microorganisms at d 25 was Candidatus Arthromitus(slow:44.5%;fast:27.4%)in small intestine.qPCR results indicated significant differences in bacterial populations between the slow and fast growing chickens,especially higher total bacteria,Enterococcus,and Clostridium cluster I in the slow growing chickens at d 25.BSH activity was higher in the slow growing chickens than the fast growing chickens[slow:0.476ΔOD/protein(μg/mL);fast:0.258ΔOD/protein(μg/mL);P<0.0001],and correlation analysis highlighted associations between BSH activity,body weight,feed intake,body weight gain,and bacterial counts.Conclusions We postulate that high total bacteria and Enterococcus abundance are associated with high BSH activity,impacting low feed intake and body weight gain,ultimately resulting in separation into slow and fast growing birds.The findings of this study contribute to understanding the relationship between gut microbiota,BSH activity,and host physiology in broiler chickens,with potential implications for poultry production.展开更多
Fasting is typically used before feeding metabolizable energy assessment in broilers.Previous studies have shown that fasting cause atrophy of the intestinal villus.Whether fasting affects intestinal permeability duri...Fasting is typically used before feeding metabolizable energy assessment in broilers.Previous studies have shown that fasting cause atrophy of the intestinal villus.Whether fasting affects intestinal permeability during refeeding by altering barrier function and nutrient absorption is of concern.Here,23-d-old broilers were randomly assigned to 5 treatments,fasted for 0,12,24,36,and 48 h,respectively,and then refed for 2 d,to study the impact of different duration of fasting on the intestinal regeneration and barrier function during refeeding.Results showed that the intestinal morphology in fasted birds was recovered in 2 d of refeeding at most.As fasting durations increased,enterocytes per intestinal villus were linearly and quadratically increased(both P<0.05),whereas goblet cells per intestinal villus was linearly decreased(both P<0.05).Besides,the mRNA level of lysozyme was linearly decreased as fasting durations increased during refeeding(both P<0.05),while quadratically increased mucin 2 was observed only after 1 d of refeeding(P<0.05).Linear increase effects were observed for claudin 2 and zonula occludens-1with increased fasting durations after 1 d of refeeding(all P<0.05),and linear and quadratical effects were observed for claudin 2 at 2 d of refeeding(both P<0.05).Besides,we found that intestinal permeability to creatinine,4 and 70 kD dextran were linearly and quadratically decreased with increased fasting durations at 6 h and 1 d of refeeding(all P<0.05).Furthermore,jejunum proteomic from birds refed for 6 h showed that birds fasted for 36 h showed increased antimicrobial peptides and upregulated retinol metabolism when compared to the nonfasted birds(P<0.05).Further study showed that retinyl ester catabolism was inhibited during fasting and enhanced during refeeding.Results of intestinal organoid culture showed that retinol benefits the cell proliferation and enterocyte differentiation.In conclusion,the intestinal permeability to small and large molecules was decreased during refeeding by strengthening the intestinal barrier function,and the activated retinol metabolism during refeeding is involved in the intestinal regeneration and strengthens the intestinal barrier.展开更多
文摘Since China began its reform and opening-up policy in the late 1970s,its economic growth has continued at an annual rate of nearly 10%.Such rapid growth derives from a few fast-growing sectors.This paper examines their growth,both overall and by sector,as well as their distribution,nature,growth momentum and impact on industrial growth and restructuring.By means of international comparative research,this paper concludes that manufacturing growth in industrialized countries is not necessarily outpaced by services.In some time periods,manufacturing tends to grow much faster than the service industry.
文摘The aim of the study was to assess the effect of natural phytoremediation, impact of growing fast-growing trees on contaminated agricultural land on ecosystem services using a Matrix evaluation system. The Matrix system is a table that links an ecosystem to its potential to provide ecosystem services. To determine the monetary price of natural capital for the provision of ecosystem services, we chose the value transfer method, based on which it is possible to assign a price (1 point = 40.7 EUR) to point values. Monitoring of the study site Kuchyňa (Mollic Fluvisol) is running since year 2010. The fast-growing willow was planted (between 2006 to 2018 year) on an area of about 43 hectares. During the monitoring of soil parameters, we observed a slight decrease in the value of the active soil reaction, a negative trend in the content of available phosphorus and a positive trend in the development of the total content risk elements in the soil. The phytoremediation ability of willow regarding risk elements was manifested by an increase in the value and price of production and regulatory ecosystem services at the monitored location. The price of natural phytoremediation of fast-growing willow on the given area was 218762.5 EUR for production services and 171509.8 EUR for regulatory services.
基金supported by research funding provided by Arm&Hammer Animal and Food Production,Waukesha,WI 53186,USA.
文摘Background Body weight is an important indicator of the overall health and production efficiency in broiler chickens.In broiler houses,body weight of chicks is variable despite the same genetics,hatching and feeding practices within a production system.The objective of this study was to investigate the intestinal microbiota and bile salt hydrolase(BSH)activity in slow and fast growing broiler chickens,which belonged to the 10th and 90th percentile body weight groups,respectively.Methods A total of 300 Ross 308 broiler chickens(100 per cohort from three independent cohorts)were selected and mucosal samples from the jejunum,ileum,and cecum were collected at day of arrival,11 and 25(n=450).Then,bacterial counts,16S rRNA amplicon sequencing,species specific real-time qPCR,as well as BSH activity were analyzed.Results Results of bacterial counts showed no significant difference between slow and fast growing cohorts(P>0.05),but they tended to be higher in the slow growing chickens in all measured bacterial groups in cecum.The 16S rRNA amplicon sequencing revealed higher relative abundance of E.coli-Shigella(71.3%−79.8%)at day of arrival,while the most abundant microorganisms at d 25 was Candidatus Arthromitus(slow:44.5%;fast:27.4%)in small intestine.qPCR results indicated significant differences in bacterial populations between the slow and fast growing chickens,especially higher total bacteria,Enterococcus,and Clostridium cluster I in the slow growing chickens at d 25.BSH activity was higher in the slow growing chickens than the fast growing chickens[slow:0.476ΔOD/protein(μg/mL);fast:0.258ΔOD/protein(μg/mL);P<0.0001],and correlation analysis highlighted associations between BSH activity,body weight,feed intake,body weight gain,and bacterial counts.Conclusions We postulate that high total bacteria and Enterococcus abundance are associated with high BSH activity,impacting low feed intake and body weight gain,ultimately resulting in separation into slow and fast growing birds.The findings of this study contribute to understanding the relationship between gut microbiota,BSH activity,and host physiology in broiler chickens,with potential implications for poultry production.
基金supported by the funding of the National Natural Science Foundation of China(32072752)the Southwest Minzu University Double World-Class Project,China(XM2023011)。
文摘Fasting is typically used before feeding metabolizable energy assessment in broilers.Previous studies have shown that fasting cause atrophy of the intestinal villus.Whether fasting affects intestinal permeability during refeeding by altering barrier function and nutrient absorption is of concern.Here,23-d-old broilers were randomly assigned to 5 treatments,fasted for 0,12,24,36,and 48 h,respectively,and then refed for 2 d,to study the impact of different duration of fasting on the intestinal regeneration and barrier function during refeeding.Results showed that the intestinal morphology in fasted birds was recovered in 2 d of refeeding at most.As fasting durations increased,enterocytes per intestinal villus were linearly and quadratically increased(both P<0.05),whereas goblet cells per intestinal villus was linearly decreased(both P<0.05).Besides,the mRNA level of lysozyme was linearly decreased as fasting durations increased during refeeding(both P<0.05),while quadratically increased mucin 2 was observed only after 1 d of refeeding(P<0.05).Linear increase effects were observed for claudin 2 and zonula occludens-1with increased fasting durations after 1 d of refeeding(all P<0.05),and linear and quadratical effects were observed for claudin 2 at 2 d of refeeding(both P<0.05).Besides,we found that intestinal permeability to creatinine,4 and 70 kD dextran were linearly and quadratically decreased with increased fasting durations at 6 h and 1 d of refeeding(all P<0.05).Furthermore,jejunum proteomic from birds refed for 6 h showed that birds fasted for 36 h showed increased antimicrobial peptides and upregulated retinol metabolism when compared to the nonfasted birds(P<0.05).Further study showed that retinyl ester catabolism was inhibited during fasting and enhanced during refeeding.Results of intestinal organoid culture showed that retinol benefits the cell proliferation and enterocyte differentiation.In conclusion,the intestinal permeability to small and large molecules was decreased during refeeding by strengthening the intestinal barrier function,and the activated retinol metabolism during refeeding is involved in the intestinal regeneration and strengthens the intestinal barrier.
