Yan Yonghe,also known as Yan Zili(1199-1267),was a famous physician of the South Song Dynasty.He lived in Nankang(present Jiujiang,Jiangxi province),and called himself“a Native of Lushan Mountain”because he lived th...Yan Yonghe,also known as Yan Zili(1199-1267),was a famous physician of the South Song Dynasty.He lived in Nankang(present Jiujiang,Jiangxi province),and called himself“a Native of Lushan Mountain”because he lived there for a long time.In the preface of the Formulas for Succouring the Sick(Ji Sheng Fang),he said he loved study since childhood,and when he was 12 years old he started to learn medicine.展开更多
Dear Editor,Porcine astrovirus(PAstV)is a swine enteric virus which can cause diarrhea and vomiting in pigs,particularly in neonatal piglets(Fang et al.,2019;Su etal.,2020).PAstVwasfirstdetectedby electron microscopyi...Dear Editor,Porcine astrovirus(PAstV)is a swine enteric virus which can cause diarrhea and vomiting in pigs,particularly in neonatal piglets(Fang et al.,2019;Su etal.,2020).PAstVwasfirstdetectedby electron microscopyinthe fecal samples from diarrheal piglets in 1980(Bridger,1980).Based on viral genomes,PAstV is divided into five distinct genotypes(PAstV1-PAstV5)(Fang et al.,2019;Mi et al.,2020;Su et al.,2020).The PAstV5 was the main genotype prevalent in China.Xiao reported a positivity rate of 24.8%for PAstV5 as the predominant genotype in Hunan Province(Xiao et al.,2017).Cai reported a positive rate of 7.5%for PAstV5 in Sichuan Province(Cai et al.,2016).A study in Yunnan Province revealed a total infection rate of 39.9%for PAstV2 and PAstV5(Ren et al.,2022).The PAstV genome open read frame 2(ORF2)encodes for a~90 kDa structural polyprotein called capsid protein(Cap)(Arias and DuBois,2017).The region including amino acids(aa)1-415(N-terminus)of the Cap ishighly conserved,and the region starting at aa 416(C-terminus)is extremely variable(Ren et al.,2022).Moreover,the Cap can bind to host cell receptors and is the main target of neutralizing antibodies(Ricemeyer et al.,2022).展开更多
Ulcerative colitis(UC)is a chronic inflammatory bowel diseasethat significantly affects the quality of life of patients.Traditional treatments often have limitations,and alternative therapies are being explored.Miao M...Ulcerative colitis(UC)is a chronic inflammatory bowel diseasethat significantly affects the quality of life of patients.Traditional treatments often have limitations,and alternative therapies are being explored.Miao Medicine,particularly Jinlong Zhi Xie Fang Enema,is a traditional herbal remedy used to treat UC symptoms,especially in patients with Large Intestine Damp-Heat Syndrome.However,clinical evidence supporting its efficacy is limited.展开更多
Jing Fang granules(JFG)are a modern Chinese medicinal formulation derived from Jing Fang Defeating Toxin San(JFDS).JFG primarily contains bioactive compounds such as flavonoids/flavonoid glycosides,coumarins and other...Jing Fang granules(JFG)are a modern Chinese medicinal formulation derived from Jing Fang Defeating Toxin San(JFDS).JFG primarily contains bioactive compounds such as flavonoids/flavonoid glycosides,coumarins and other components,which have various activities:anti-inflammatory,immune system regulation,antiviral,anti-aging,detoxification,and protection of the liver,lungs,and kidneys.JFG has broad potential therapeutic applications.The mechanisms of JFG’s actions are primarily associated with signaling pathways such as TLR4/NF-κB,PI3K/AKT,and MAPK.This article reviews the research progress on JFG,including chemical composition,pharmacologic effects,mechanism of action,safety,clinical application,etc.,serving as a foundation for future research and clinical applications.展开更多
The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, ...The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.展开更多
Fanged frogs, now called Limnonectes kuhlii, from Borneo are remotely related to true Javanese L. kuhliL For future taxonomy of Bornean fanged frogs, we fix the nomenclatural status of two existing names, Rana conspic...Fanged frogs, now called Limnonectes kuhlii, from Borneo are remotely related to true Javanese L. kuhliL For future taxonomy of Bornean fanged frogs, we fix the nomenclatural status of two existing names, Rana conspicillata Giunther, 1872 and Rana paradoxa Mocquard, 1890. Morphological comparison of the type-series revealed heterospecific relationships of the two species. For R. conspicillata, we designate BMNH 1947.2.29.20 as the lectotype, and for R. para doxa, we also designate MNHN 1889.222 as the lectotype and propose a replacement name Limnonectes mocquardi, in order to stabilize their nomenclature.展开更多
AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1...AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine(5-HT) and substance P(SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity.in normal rats and 4.5 ± 1.58 in IBS model rats(P < 0.001). However, the defecation frequency was significantly decreased(3.0 ± 1.25 vs 4.5 ± 1.58, P < 0.05), while the time(in seconds) of colon transit function was significantly increased(256.88 ± 20.32 vs 93.36 ± 17.28, P < 0.001) in IBS + TXYF group rats than in IBS group rats. Increased colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation. CONCLUSION: TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS.展开更多
BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointesti...BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS),is a common functional bowel disease.Its main symptoms are recurrent abdominal pain,diarrhea,constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes.By comparing the body fluid metabolism maps of model animals,metabolomics can discover disease biomarkers,analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body.Thus,the evaluation of animal models tends to be comprehensive and objective.This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models,and then randomly divided into four groups:A model control group and three Tong Xie Yao Fang treatment groups(high,medium and low doses).A normal,non-IBS group was established.The rats were treated for 2 wk.On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Nine potential biomarkers were identified,and six major metabolic pathways were found to be related to IBS.RESULTS In the study of metabonomics,nine potential biomarkers including L-serine,4-methylgallic acid,L-threonine,succinylacetone,prolyl-hydroxyproline,valylserine,acetyl citrate,marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine,which were assigned to amino acids,organic acids,succinyl and glycosides.Furthermore,the metabolic pathway of L-serine,L-threonine and 5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and Genomes,which mainly involved the metabolism of cysteine and methionine,vitamin B6 metabolism,serotonin synapse,tryptophan metabolism,sphingolipid metabolism,digestion,absorption of protein and amino acid metabolism.These pathways are related to intestinal dysfunction,inflammatory syndrome,nervous system dysfunction and other diseases.CONCLUSION Tong Xie Yao Fang has pharmacological effects on IBS,and its mechanism may be related to the metabolism of the nine potential biomarkers identified above in urine.展开更多
文摘Yan Yonghe,also known as Yan Zili(1199-1267),was a famous physician of the South Song Dynasty.He lived in Nankang(present Jiujiang,Jiangxi province),and called himself“a Native of Lushan Mountain”because he lived there for a long time.In the preface of the Formulas for Succouring the Sick(Ji Sheng Fang),he said he loved study since childhood,and when he was 12 years old he started to learn medicine.
基金supported by grants from the Henan Province Key Research and Development Special Project(231111113100)the Henan Province Natural Science Foundation Interdisciplinary Innovation Research Group Project(232300421001)the Postgraduate Education Reform and Quality Improvement Project of Henan Province(YJS2023SZ13).
文摘Dear Editor,Porcine astrovirus(PAstV)is a swine enteric virus which can cause diarrhea and vomiting in pigs,particularly in neonatal piglets(Fang et al.,2019;Su etal.,2020).PAstVwasfirstdetectedby electron microscopyinthe fecal samples from diarrheal piglets in 1980(Bridger,1980).Based on viral genomes,PAstV is divided into five distinct genotypes(PAstV1-PAstV5)(Fang et al.,2019;Mi et al.,2020;Su et al.,2020).The PAstV5 was the main genotype prevalent in China.Xiao reported a positivity rate of 24.8%for PAstV5 as the predominant genotype in Hunan Province(Xiao et al.,2017).Cai reported a positive rate of 7.5%for PAstV5 in Sichuan Province(Cai et al.,2016).A study in Yunnan Province revealed a total infection rate of 39.9%for PAstV2 and PAstV5(Ren et al.,2022).The PAstV genome open read frame 2(ORF2)encodes for a~90 kDa structural polyprotein called capsid protein(Cap)(Arias and DuBois,2017).The region including amino acids(aa)1-415(N-terminus)of the Cap ishighly conserved,and the region starting at aa 416(C-terminus)is extremely variable(Ren et al.,2022).Moreover,the Cap can bind to host cell receptors and is the main target of neutralizing antibodies(Ricemeyer et al.,2022).
基金Research Topic on traditional Chinese medicine and ethnic medicine science and technology of Guizhou Provincial Administration of Traditional Chinese Medicine(Project No.:QZYY-2024-100)。
文摘Ulcerative colitis(UC)is a chronic inflammatory bowel diseasethat significantly affects the quality of life of patients.Traditional treatments often have limitations,and alternative therapies are being explored.Miao Medicine,particularly Jinlong Zhi Xie Fang Enema,is a traditional herbal remedy used to treat UC symptoms,especially in patients with Large Intestine Damp-Heat Syndrome.However,clinical evidence supporting its efficacy is limited.
基金supported by the Key R&D Program of Shandong Province,China(No.2024TZXD067,2022SFGC0105)Natural Science Foundation of Shandong Province(No.ZR2023QB021)+2 种基金Young Taishan Scholarship to Xuekui Xia(No.tsqn202103100)Jinan Talent Project for Universities(No.2021GXRC062,202228088)Key innovation Project and Science,Education and Industry Integration Innovation Pilot Project of Qilu University of Technology(Shandong Academy of Sciences)(No.2022JBZ01-06,2024ZDZX03,2023RCKY214).
文摘Jing Fang granules(JFG)are a modern Chinese medicinal formulation derived from Jing Fang Defeating Toxin San(JFDS).JFG primarily contains bioactive compounds such as flavonoids/flavonoid glycosides,coumarins and other components,which have various activities:anti-inflammatory,immune system regulation,antiviral,anti-aging,detoxification,and protection of the liver,lungs,and kidneys.JFG has broad potential therapeutic applications.The mechanisms of JFG’s actions are primarily associated with signaling pathways such as TLR4/NF-κB,PI3K/AKT,and MAPK.This article reviews the research progress on JFG,including chemical composition,pharmacologic effects,mechanism of action,safety,clinical application,etc.,serving as a foundation for future research and clinical applications.
基金Project (2010FA32370) supported by The Ministry of Science and Technology of ChinaProject (2008WK3002) supported by Hunan Provincial Science and Technology DepartmentProject (20060390891) supported by the Postdoctoral Science Foundation of China
文摘The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.
基金Examination of museum specimens by MM was made possible by grants from the Monbusho International Scientific Research Programs(Field Research Nos.01041051,02041051)
文摘Fanged frogs, now called Limnonectes kuhlii, from Borneo are remotely related to true Javanese L. kuhliL For future taxonomy of Bornean fanged frogs, we fix the nomenclatural status of two existing names, Rana conspicillata Giunther, 1872 and Rana paradoxa Mocquard, 1890. Morphological comparison of the type-series revealed heterospecific relationships of the two species. For R. conspicillata, we designate BMNH 1947.2.29.20 as the lectotype, and for R. para doxa, we also designate MNHN 1889.222 as the lectotype and propose a replacement name Limnonectes mocquardi, in order to stabilize their nomenclature.
基金Supported by National Education Department"ChunHui Plan"Research Projects,No.Z2010021China Postdoctoral Science Foundation Project,No.2013M531079+2 种基金Heilongjiang Postdoctoral Funding Project,No.LBH-Z12246Heilongjiang Education Department Scientific Research Project,No.12521502excellent Innovative Talents Support Program Funding of Heilongjiang University of Chinese Medicine(Outstanding Young Academic Leaders),No.051217
文摘AIM: To investigate whether the Chinese medicine Tong Xie Yao Fang(TXYF) improves dysfunction in an irritable bowel syndrome(IBS) rat model. METHODS: Thirty baby rats for IBS modeling were separated from mother rats(1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine(5-HT) and substance P(SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity.in normal rats and 4.5 ± 1.58 in IBS model rats(P < 0.001). However, the defecation frequency was significantly decreased(3.0 ± 1.25 vs 4.5 ± 1.58, P < 0.05), while the time(in seconds) of colon transit function was significantly increased(256.88 ± 20.32 vs 93.36 ± 17.28, P < 0.001) in IBS + TXYF group rats than in IBS group rats. Increased colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation. CONCLUSION: TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS.
基金Supported by the National Natural Science Foundation of China,No.81573870the Eighth Special Subsidy Project of China Post Doctoral Science Foundation,No.2015T80376+4 种基金Postdoctoral Science Research Developmental Foundation of China,No.2013M531079National Science Foundation of Heilongjiang Province,No.H2015020Heilongjiang University of Traditional Chinese Medicine Outstanding Innovative Talents Support Project(Outstanding Young Academic Leaders),Postdoctoral Science-Research Developmental Foundation of Heilongjiang Province,No.LBHQ12009Youth Academic Backbone Fund of Heilongjiang Province Education Department,No.1253G053Youth Science and Technology Project of Traditional Chinese Medicine Department of Heilongjiang Province,No.ZQG-034
文摘BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS),is a common functional bowel disease.Its main symptoms are recurrent abdominal pain,diarrhea,constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes.By comparing the body fluid metabolism maps of model animals,metabolomics can discover disease biomarkers,analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body.Thus,the evaluation of animal models tends to be comprehensive and objective.This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models,and then randomly divided into four groups:A model control group and three Tong Xie Yao Fang treatment groups(high,medium and low doses).A normal,non-IBS group was established.The rats were treated for 2 wk.On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Nine potential biomarkers were identified,and six major metabolic pathways were found to be related to IBS.RESULTS In the study of metabonomics,nine potential biomarkers including L-serine,4-methylgallic acid,L-threonine,succinylacetone,prolyl-hydroxyproline,valylserine,acetyl citrate,marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine,which were assigned to amino acids,organic acids,succinyl and glycosides.Furthermore,the metabolic pathway of L-serine,L-threonine and 5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and Genomes,which mainly involved the metabolism of cysteine and methionine,vitamin B6 metabolism,serotonin synapse,tryptophan metabolism,sphingolipid metabolism,digestion,absorption of protein and amino acid metabolism.These pathways are related to intestinal dysfunction,inflammatory syndrome,nervous system dysfunction and other diseases.CONCLUSION Tong Xie Yao Fang has pharmacological effects on IBS,and its mechanism may be related to the metabolism of the nine potential biomarkers identified above in urine.
