克隆合作猪神经营养因子3基因(Neurotrophin3,NTF3)的编码区序列(Coding region sequence,CDS),预测分析其结构与功能,并检测基因在不同组织中的表达量,构建组织表达谱。通过PCR扩增、Sanger测序等方法获得合作猪NTF3基因CDS区序列,使...克隆合作猪神经营养因子3基因(Neurotrophin3,NTF3)的编码区序列(Coding region sequence,CDS),预测分析其结构与功能,并检测基因在不同组织中的表达量,构建组织表达谱。通过PCR扩增、Sanger测序等方法获得合作猪NTF3基因CDS区序列,使用生物信息学方法分析其结构,并使用RT-qPCR技术检测其在不同组织中的表达水平。结果显示,合作猪NTF3基因CDS区长774 bp,编码257个氨基酸;等电点(pI)为9.46,属于碱性蛋白;合作猪与猪、牛亲缘关系较近;二级结构主要为α-螺旋和无规则卷曲,三级结构主要由β-折叠、α-螺旋等组成;整条链中亲水性氨基酸残基数量多于疏水性氨基酸残基数量,是亲水性蛋白;无跨膜区域,是非跨膜蛋白;在37~58位氨基酸残基有一个线圈结构域、64~79位有一个低复杂度结构域和144~249位有一个神经生长因子结构域;存在信号肽,推测是分泌蛋白;NTF3基因在合作猪脾脏的表达量最高,回肠表达量最低。展开更多
Dear Editor,This letter presents a novel latent factorization model for high dimensional and incomplete (HDI) tensor, namely the neural Tucker factorization (Neu Tuc F), which is a generic neural network-based latent-...Dear Editor,This letter presents a novel latent factorization model for high dimensional and incomplete (HDI) tensor, namely the neural Tucker factorization (Neu Tuc F), which is a generic neural network-based latent-factorization-of-tensors model under the Tucker decomposition framework.展开更多
Substantial effects of photochemical reaction losses of volatile organic compounds(VOCs)on factor profiles can be investigated by comparing the differences between daytime and nighttime dispersion-normalized VOC data ...Substantial effects of photochemical reaction losses of volatile organic compounds(VOCs)on factor profiles can be investigated by comparing the differences between daytime and nighttime dispersion-normalized VOC data resolved profiles.Hourly speciated VOC data measured in Shijiazhuang,China from May to September 2021 were used to conduct study.The mean VOC concentration in the daytime and at nighttime were 32.8 and 36.0 ppbv,respectively.Alkanes and aromatics concentrations in the daytime(12.9 and 3.08 ppbv)were lower than nighttime(15.5 and 3.63 ppbv),whereas that of alkenes showed the opposite tendency.The concentration differences between daytime and nighttime for alkynes and halogenated hydrocarbonswere uniformly small.The reactivities of the dominant species in factor profiles for gasoline emissions,natural gas and diesel vehicles,and liquefied petroleum gas were relatively low and their profiles were less affected by photochemical losses.Photochemical losses produced a substantial impact on the profiles of solvent use,petrochemical industry emissions,combustion sources,and biogenic emissions where the dominant species in these factor profiles had high reactivities.Although the profile of biogenic emissions was substantially affected by photochemical loss of isoprene,the low emissions at nighttime also had an important impact on its profile.Chemical losses of highly active VOC species substantially reduced their concentrations in apportioned factor profiles.This study results were consistent with the analytical results obtained through initial concentration estimation,suggesting that the initial concentration estimation could be the most effective currently availablemethod for the source analyses of active VOCs although with uncertainty.展开更多
Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationa...Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationally,this study delves into the mechanism by which data governance promotes data factorization and proposes implementation paths for data governance oriented toward data factorization.The aim is to facilitate the intelligent transformation and high-quality development of libraries.展开更多
CircRNAs,widely found throughout the human bodies,play a crucial role in regulating various biological processes and are closely linked to complex human diseases.Investigating potential associations between circRNAs a...CircRNAs,widely found throughout the human bodies,play a crucial role in regulating various biological processes and are closely linked to complex human diseases.Investigating potential associations between circRNAs and diseases can enhance our understanding of diseases and provide new strategies and tools for early diagnosis,treatment,and disease prevention.However,existing models have limitations in accurately capturing similarities,handling the sparse and noise attributes of association networks,and fully leveraging bioinformatical aspects from multiple viewpoints.To address these issues,this study introduces a new non-negative matrix factorization-based framework called NMFMSN.First,we incorporate circRNA sequence data and disease semantic information to compute circRNA and disease similarity,respectively.Given the sparse known associations between circRNAs and diseases,we reconstruct the network to complete more associations by imputing missing links based on neighboring circRNA and disease interactions.Finally,we integrate these two similarity networks into a non-negative matrix factorization framework to identify potential circRNA-disease associations.Upon conducting 5-fold cross-validation and leave-one-out cross-validation,the AUC values for NMFMSN reach 0.9712 and 0.9768,respectively,outperforming the currently most advanced models.Case studies on lung cancer and hepatocellular carcinoma show that NMFMSN is a good way to predict new associations between circRNAs and diseases.展开更多
In this paper,a nonlinear control approach for an unstable networked plant in the presence of actuator and sensor limitations using robust right coprime factorization is proposed.The actuator is limited by upper and l...In this paper,a nonlinear control approach for an unstable networked plant in the presence of actuator and sensor limitations using robust right coprime factorization is proposed.The actuator is limited by upper and lower constraints and the sensor in the feedback loop is subjected to network-induced unknown time-varying delay and noise.With this nonlinear control method,we first employ right coprime factorization based on isomorphism and operator theory to factorize the plant,so that bounded input bounded output(BIBO)stability can be guaranteed.Next,continuous-time generalized predictive control(CGPC)is utilized for the unstable operator of the right coprime factorized plant to guarantee inner stability and enables the closed-loop dynamics of the system with predictive characteristics.Meanwhile,a second-Do F(degrees of freedom)switched controller that satisfies a perturbed Bezout identity and a robustness condition is designed.By using the CGPC controller that possesses predictive behavior and the second-Do F switched stabilizer,the overall stability of the plant subjected to actuator limitations is guaranteed.To address sensor limitations that exist in networked plants in the form of delay and noise which often cause system performance degradation,we implement an identity operator definition in the feedback loop to compensate for these adverse effects.Further,a pre-operator is designed to ensure that the plant output tracks the reference input.Finally,the effectiveness of the proposed design scheme is demonstrated by simulations.展开更多
Fine particulatematter(PM_(2.5))samples were collected in two neighboring cities,Beijing and Baoding,China.High-concentration events of PM_(2.5) in which the average mass concentration exceeded 75μg/m^(3) were freque...Fine particulatematter(PM_(2.5))samples were collected in two neighboring cities,Beijing and Baoding,China.High-concentration events of PM_(2.5) in which the average mass concentration exceeded 75μg/m^(3) were frequently observed during the heating season.Dispersion Normalized Positive Matrix Factorization was applied for the source apportionment of PM_(2.5) as minimize the dilution effects of meteorology and better reflect the source strengths in these two cities.Secondary nitrate had the highest contribution for Beijing(37.3%),and residential heating/biomass burning was the largest for Baoding(27.1%).Secondary nitrate,mobile,biomass burning,district heating,oil combustion,aged sea salt sources showed significant differences between the heating and non-heating seasons in Beijing for same period(2019.01.10–2019.08.22)(Mann-Whitney Rank Sum Test P<0.05).In case of Baoding,soil,residential heating/biomass burning,incinerator,coal combustion,oil combustion sources showed significant differences.The results of Pearson correlation analysis for the common sources between the two cities showed that long-range transported sources and some sources with seasonal patterns such as oil combustion and soil had high correlation coefficients.Conditional Bivariate Probability Function(CBPF)was used to identify the inflow directions for the sources,and joint-PSCF(Potential Source Contribution Function)was performed to determine the common potential source areas for sources affecting both cities.These models facilitated a more precise verification of city-specific influences on PM_(2.5) sources.The results of this study will aid in prioritizing air pollution mitigation strategies during the heating season and strengthening air quality management to reduce the impact of downwind neighboring cities.展开更多
Dear Editor,This letter presents a latent-factorization-of-tensors(LFT)-incorporated battery cycle life prediction framework.Data-driven prognosis and health management(PHM)for battery pack(BP)can boost the safety and...Dear Editor,This letter presents a latent-factorization-of-tensors(LFT)-incorporated battery cycle life prediction framework.Data-driven prognosis and health management(PHM)for battery pack(BP)can boost the safety and sustainability of a battery management system(BMS),which relies heavily on the quality of the measured BP data like the voltage(V),current(I),and temperature(T).展开更多
介绍NTF(Noise Transfer Function)分析在设计开发阶段和试验验证阶段的应用,重点包括目标值设定方法、仿真模型搭建方法、模型精度提高方法、仿真优化步骤、试验测量方法.提出优化仿真NTF曲线峰值的PSA(Panel Structure Acoustics)方法...介绍NTF(Noise Transfer Function)分析在设计开发阶段和试验验证阶段的应用,重点包括目标值设定方法、仿真模型搭建方法、模型精度提高方法、仿真优化步骤、试验测量方法.提出优化仿真NTF曲线峰值的PSA(Panel Structure Acoustics)方法;运用此方法快速定位导致NTF曲线峰值的问题板件及变形模式,有针对性地对其优化设计,给出PSA法在设计阶段和试验验证阶段使用案例,验证其有效性.展开更多
文摘克隆合作猪神经营养因子3基因(Neurotrophin3,NTF3)的编码区序列(Coding region sequence,CDS),预测分析其结构与功能,并检测基因在不同组织中的表达量,构建组织表达谱。通过PCR扩增、Sanger测序等方法获得合作猪NTF3基因CDS区序列,使用生物信息学方法分析其结构,并使用RT-qPCR技术检测其在不同组织中的表达水平。结果显示,合作猪NTF3基因CDS区长774 bp,编码257个氨基酸;等电点(pI)为9.46,属于碱性蛋白;合作猪与猪、牛亲缘关系较近;二级结构主要为α-螺旋和无规则卷曲,三级结构主要由β-折叠、α-螺旋等组成;整条链中亲水性氨基酸残基数量多于疏水性氨基酸残基数量,是亲水性蛋白;无跨膜区域,是非跨膜蛋白;在37~58位氨基酸残基有一个线圈结构域、64~79位有一个低复杂度结构域和144~249位有一个神经生长因子结构域;存在信号肽,推测是分泌蛋白;NTF3基因在合作猪脾脏的表达量最高,回肠表达量最低。
基金supported by the National Natural Science Foundation of China(62272078)Chongqing Natural Science Foundation(CSTB2023NSCQ-LZX0069)the Science and Technology Research Program of Chongqing Municipal Education Commission(KJQN202300210)
文摘Dear Editor,This letter presents a novel latent factorization model for high dimensional and incomplete (HDI) tensor, namely the neural Tucker factorization (Neu Tuc F), which is a generic neural network-based latent-factorization-of-tensors model under the Tucker decomposition framework.
基金supported by the National Key R&D Program of China(No.2023YFC3705801)the National Natural Science Foundation of China(No.42177085).
文摘Substantial effects of photochemical reaction losses of volatile organic compounds(VOCs)on factor profiles can be investigated by comparing the differences between daytime and nighttime dispersion-normalized VOC data resolved profiles.Hourly speciated VOC data measured in Shijiazhuang,China from May to September 2021 were used to conduct study.The mean VOC concentration in the daytime and at nighttime were 32.8 and 36.0 ppbv,respectively.Alkanes and aromatics concentrations in the daytime(12.9 and 3.08 ppbv)were lower than nighttime(15.5 and 3.63 ppbv),whereas that of alkenes showed the opposite tendency.The concentration differences between daytime and nighttime for alkynes and halogenated hydrocarbonswere uniformly small.The reactivities of the dominant species in factor profiles for gasoline emissions,natural gas and diesel vehicles,and liquefied petroleum gas were relatively low and their profiles were less affected by photochemical losses.Photochemical losses produced a substantial impact on the profiles of solvent use,petrochemical industry emissions,combustion sources,and biogenic emissions where the dominant species in these factor profiles had high reactivities.Although the profile of biogenic emissions was substantially affected by photochemical loss of isoprene,the low emissions at nighttime also had an important impact on its profile.Chemical losses of highly active VOC species substantially reduced their concentrations in apportioned factor profiles.This study results were consistent with the analytical results obtained through initial concentration estimation,suggesting that the initial concentration estimation could be the most effective currently availablemethod for the source analyses of active VOCs although with uncertainty.
文摘Data factors are becoming the core driving force in the intelligent transformation of libraries.Based on a systematic review of the progress in data governance practices in libraries both domestically and internationally,this study delves into the mechanism by which data governance promotes data factorization and proposes implementation paths for data governance oriented toward data factorization.The aim is to facilitate the intelligent transformation and high-quality development of libraries.
基金the Gansu Province Industrial Support Plan(No.2023CYZC-25)Natural Science Foundation of Gansu Province(No.23JRRA770)the National Natural Science Foundation of China(No.62162040)。
文摘CircRNAs,widely found throughout the human bodies,play a crucial role in regulating various biological processes and are closely linked to complex human diseases.Investigating potential associations between circRNAs and diseases can enhance our understanding of diseases and provide new strategies and tools for early diagnosis,treatment,and disease prevention.However,existing models have limitations in accurately capturing similarities,handling the sparse and noise attributes of association networks,and fully leveraging bioinformatical aspects from multiple viewpoints.To address these issues,this study introduces a new non-negative matrix factorization-based framework called NMFMSN.First,we incorporate circRNA sequence data and disease semantic information to compute circRNA and disease similarity,respectively.Given the sparse known associations between circRNAs and diseases,we reconstruct the network to complete more associations by imputing missing links based on neighboring circRNA and disease interactions.Finally,we integrate these two similarity networks into a non-negative matrix factorization framework to identify potential circRNA-disease associations.Upon conducting 5-fold cross-validation and leave-one-out cross-validation,the AUC values for NMFMSN reach 0.9712 and 0.9768,respectively,outperforming the currently most advanced models.Case studies on lung cancer and hepatocellular carcinoma show that NMFMSN is a good way to predict new associations between circRNAs and diseases.
文摘In this paper,a nonlinear control approach for an unstable networked plant in the presence of actuator and sensor limitations using robust right coprime factorization is proposed.The actuator is limited by upper and lower constraints and the sensor in the feedback loop is subjected to network-induced unknown time-varying delay and noise.With this nonlinear control method,we first employ right coprime factorization based on isomorphism and operator theory to factorize the plant,so that bounded input bounded output(BIBO)stability can be guaranteed.Next,continuous-time generalized predictive control(CGPC)is utilized for the unstable operator of the right coprime factorized plant to guarantee inner stability and enables the closed-loop dynamics of the system with predictive characteristics.Meanwhile,a second-Do F(degrees of freedom)switched controller that satisfies a perturbed Bezout identity and a robustness condition is designed.By using the CGPC controller that possesses predictive behavior and the second-Do F switched stabilizer,the overall stability of the plant subjected to actuator limitations is guaranteed.To address sensor limitations that exist in networked plants in the form of delay and noise which often cause system performance degradation,we implement an identity operator definition in the feedback loop to compensate for these adverse effects.Further,a pre-operator is designed to ensure that the plant output tracks the reference input.Finally,the effectiveness of the proposed design scheme is demonstrated by simulations.
基金supported by the National Institute of Environmental Research(NIER)funded by the Ministry of Environment(No.NIER-2019-04-02-039)supported by Particulate Matter Management Specialized Graduate Program through the Korea Environmental Industry&Technology Institute(KEITI)funded by the Ministry of Environment(MOE).
文摘Fine particulatematter(PM_(2.5))samples were collected in two neighboring cities,Beijing and Baoding,China.High-concentration events of PM_(2.5) in which the average mass concentration exceeded 75μg/m^(3) were frequently observed during the heating season.Dispersion Normalized Positive Matrix Factorization was applied for the source apportionment of PM_(2.5) as minimize the dilution effects of meteorology and better reflect the source strengths in these two cities.Secondary nitrate had the highest contribution for Beijing(37.3%),and residential heating/biomass burning was the largest for Baoding(27.1%).Secondary nitrate,mobile,biomass burning,district heating,oil combustion,aged sea salt sources showed significant differences between the heating and non-heating seasons in Beijing for same period(2019.01.10–2019.08.22)(Mann-Whitney Rank Sum Test P<0.05).In case of Baoding,soil,residential heating/biomass burning,incinerator,coal combustion,oil combustion sources showed significant differences.The results of Pearson correlation analysis for the common sources between the two cities showed that long-range transported sources and some sources with seasonal patterns such as oil combustion and soil had high correlation coefficients.Conditional Bivariate Probability Function(CBPF)was used to identify the inflow directions for the sources,and joint-PSCF(Potential Source Contribution Function)was performed to determine the common potential source areas for sources affecting both cities.These models facilitated a more precise verification of city-specific influences on PM_(2.5) sources.The results of this study will aid in prioritizing air pollution mitigation strategies during the heating season and strengthening air quality management to reduce the impact of downwind neighboring cities.
文摘Dear Editor,This letter presents a latent-factorization-of-tensors(LFT)-incorporated battery cycle life prediction framework.Data-driven prognosis and health management(PHM)for battery pack(BP)can boost the safety and sustainability of a battery management system(BMS),which relies heavily on the quality of the measured BP data like the voltage(V),current(I),and temperature(T).
文摘介绍NTF(Noise Transfer Function)分析在设计开发阶段和试验验证阶段的应用,重点包括目标值设定方法、仿真模型搭建方法、模型精度提高方法、仿真优化步骤、试验测量方法.提出优化仿真NTF曲线峰值的PSA(Panel Structure Acoustics)方法;运用此方法快速定位导致NTF曲线峰值的问题板件及变形模式,有针对性地对其优化设计,给出PSA法在设计阶段和试验验证阶段使用案例,验证其有效性.
