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合作猪NTF3基因克隆、生物信息学分析及组织表达谱构建
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作者 闫尊强 鲁亚伟 +1 位作者 滚双宝 王鹏飞 《西北农业学报》 北大核心 2025年第1期1-9,共9页
克隆合作猪神经营养因子3基因(Neurotrophin3,NTF3)的编码区序列(Coding region sequence,CDS),预测分析其结构与功能,并检测基因在不同组织中的表达量,构建组织表达谱。通过PCR扩增、Sanger测序等方法获得合作猪NTF3基因CDS区序列,使... 克隆合作猪神经营养因子3基因(Neurotrophin3,NTF3)的编码区序列(Coding region sequence,CDS),预测分析其结构与功能,并检测基因在不同组织中的表达量,构建组织表达谱。通过PCR扩增、Sanger测序等方法获得合作猪NTF3基因CDS区序列,使用生物信息学方法分析其结构,并使用RT-qPCR技术检测其在不同组织中的表达水平。结果显示,合作猪NTF3基因CDS区长774 bp,编码257个氨基酸;等电点(pI)为9.46,属于碱性蛋白;合作猪与猪、牛亲缘关系较近;二级结构主要为α-螺旋和无规则卷曲,三级结构主要由β-折叠、α-螺旋等组成;整条链中亲水性氨基酸残基数量多于疏水性氨基酸残基数量,是亲水性蛋白;无跨膜区域,是非跨膜蛋白;在37~58位氨基酸残基有一个线圈结构域、64~79位有一个低复杂度结构域和144~249位有一个神经生长因子结构域;存在信号肽,推测是分泌蛋白;NTF3基因在合作猪脾脏的表达量最高,回肠表达量最低。 展开更多
关键词 合作猪 ntf3基因 克隆 生物信息学分析
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NTF2在恶性肿瘤发生和治疗中的研究进展
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作者 罗红玉 王健力 +2 位作者 田彩平 魏政丽 毛爱红 《甘肃医药》 2025年第1期9-12,25,共5页
核转运因子2(NTF2)是一种参与核运输的细胞质因子,通过与Ran-GDP结合参与生物大分子核质运输过程,负责将特定蛋白质从细胞质转运至细胞核。近年来,随着对恶性肿瘤发生发展机制的深入研究,越来越多的证据表明,NTF2在多种恶性肿瘤中异常... 核转运因子2(NTF2)是一种参与核运输的细胞质因子,通过与Ran-GDP结合参与生物大分子核质运输过程,负责将特定蛋白质从细胞质转运至细胞核。近年来,随着对恶性肿瘤发生发展机制的深入研究,越来越多的证据表明,NTF2在多种恶性肿瘤中异常表达。NTF2可通过调控核大小及信号转导通路参与细胞周期、凋亡及免疫应答等细胞过程,在肿瘤发生发展和转移过程中发挥关键的推动作用。本文就NTF2的生物学特性、转运机制、异常表达及其参与的信号通路进行综述,并进一步揭示NTF2潜在的治疗可能性,旨在为恶性肿瘤靶向治疗提供一种新思路。 展开更多
关键词 ntf2 恶性肿瘤 生物标志物 靶向治疗
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Neural Tucker Factorization
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作者 Peng Tang Xin Luo 《IEEE/CAA Journal of Automatica Sinica》 2025年第2期475-477,共3页
Dear Editor,This letter presents a novel latent factorization model for high dimensional and incomplete (HDI) tensor, namely the neural Tucker factorization (Neu Tuc F), which is a generic neural network-based latent-... Dear Editor,This letter presents a novel latent factorization model for high dimensional and incomplete (HDI) tensor, namely the neural Tucker factorization (Neu Tuc F), which is a generic neural network-based latent-factorization-of-tensors model under the Tucker decomposition framework. 展开更多
关键词 neu tuc f neural tucker factorization latent factorization model high dimensional tensor tucker decomposition framework neural network incomplete tensor latent factorization
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Changes in factor profiles deriving from photochemical losses of volatile organic compounds:Insight from daytime and nighttime positive matrix factorization ana
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作者 Baoshuang Liu Tao Yang +9 位作者 Sicong Kang Fuquan Wang Haixu Zhang Man Xu Wei Wang Jinrui Bai Shaojie Song Qili Dai Yinchang Feng Philip K.Hopke 《Journal of Environmental Sciences》 2025年第5期627-639,共13页
Substantial effects of photochemical reaction losses of volatile organic compounds(VOCs)on factor profiles can be investigated by comparing the differences between daytime and nighttime dispersion-normalized VOC data ... Substantial effects of photochemical reaction losses of volatile organic compounds(VOCs)on factor profiles can be investigated by comparing the differences between daytime and nighttime dispersion-normalized VOC data resolved profiles.Hourly speciated VOC data measured in Shijiazhuang,China from May to September 2021 were used to conduct study.The mean VOC concentration in the daytime and at nighttime were 32.8 and 36.0 ppbv,respectively.Alkanes and aromatics concentrations in the daytime(12.9 and 3.08 ppbv)were lower than nighttime(15.5 and 3.63 ppbv),whereas that of alkenes showed the opposite tendency.The concentration differences between daytime and nighttime for alkynes and halogenated hydrocarbonswere uniformly small.The reactivities of the dominant species in factor profiles for gasoline emissions,natural gas and diesel vehicles,and liquefied petroleum gas were relatively low and their profiles were less affected by photochemical losses.Photochemical losses produced a substantial impact on the profiles of solvent use,petrochemical industry emissions,combustion sources,and biogenic emissions where the dominant species in these factor profiles had high reactivities.Although the profile of biogenic emissions was substantially affected by photochemical loss of isoprene,the low emissions at nighttime also had an important impact on its profile.Chemical losses of highly active VOC species substantially reduced their concentrations in apportioned factor profiles.This study results were consistent with the analytical results obtained through initial concentration estimation,suggesting that the initial concentration estimation could be the most effective currently availablemethod for the source analyses of active VOCs although with uncertainty. 展开更多
关键词 Volatile organic compounds Dispersion normalization Photochemical loss Factor profile Positive matrix factorization
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Predicting CircRNA-Disease Associations via Non-Negative Matrix Factorization Fused with Multiple Similarity Networks
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作者 LU Pengli LI Shiying 《Journal of Shanghai Jiaotong university(Science)》 2025年第4期709-719,共11页
CircRNAs,widely found throughout the human bodies,play a crucial role in regulating various biological processes and are closely linked to complex human diseases.Investigating potential associations between circRNAs a... CircRNAs,widely found throughout the human bodies,play a crucial role in regulating various biological processes and are closely linked to complex human diseases.Investigating potential associations between circRNAs and diseases can enhance our understanding of diseases and provide new strategies and tools for early diagnosis,treatment,and disease prevention.However,existing models have limitations in accurately capturing similarities,handling the sparse and noise attributes of association networks,and fully leveraging bioinformatical aspects from multiple viewpoints.To address these issues,this study introduces a new non-negative matrix factorization-based framework called NMFMSN.First,we incorporate circRNA sequence data and disease semantic information to compute circRNA and disease similarity,respectively.Given the sparse known associations between circRNAs and diseases,we reconstruct the network to complete more associations by imputing missing links based on neighboring circRNA and disease interactions.Finally,we integrate these two similarity networks into a non-negative matrix factorization framework to identify potential circRNA-disease associations.Upon conducting 5-fold cross-validation and leave-one-out cross-validation,the AUC values for NMFMSN reach 0.9712 and 0.9768,respectively,outperforming the currently most advanced models.Case studies on lung cancer and hepatocellular carcinoma show that NMFMSN is a good way to predict new associations between circRNAs and diseases. 展开更多
关键词 circRNA-disease associations circRNA sequence data disease semantic information non-negative matrix factorization
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Nonlinear Control for Unstable Networked Plants in the Presence of Actuator and Sensor Limitations Using Robust Right Coprime Factorization
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作者 Yuanhong Xu Mingcong Deng 《IEEE/CAA Journal of Automatica Sinica》 2025年第3期516-527,共12页
In this paper,a nonlinear control approach for an unstable networked plant in the presence of actuator and sensor limitations using robust right coprime factorization is proposed.The actuator is limited by upper and l... In this paper,a nonlinear control approach for an unstable networked plant in the presence of actuator and sensor limitations using robust right coprime factorization is proposed.The actuator is limited by upper and lower constraints and the sensor in the feedback loop is subjected to network-induced unknown time-varying delay and noise.With this nonlinear control method,we first employ right coprime factorization based on isomorphism and operator theory to factorize the plant,so that bounded input bounded output(BIBO)stability can be guaranteed.Next,continuous-time generalized predictive control(CGPC)is utilized for the unstable operator of the right coprime factorized plant to guarantee inner stability and enables the closed-loop dynamics of the system with predictive characteristics.Meanwhile,a second-Do F(degrees of freedom)switched controller that satisfies a perturbed Bezout identity and a robustness condition is designed.By using the CGPC controller that possesses predictive behavior and the second-Do F switched stabilizer,the overall stability of the plant subjected to actuator limitations is guaranteed.To address sensor limitations that exist in networked plants in the form of delay and noise which often cause system performance degradation,we implement an identity operator definition in the feedback loop to compensate for these adverse effects.Further,a pre-operator is designed to ensure that the plant output tracks the reference input.Finally,the effectiveness of the proposed design scheme is demonstrated by simulations. 展开更多
关键词 Actuator and sensor limitations identity operator definition network-induced limitations robust right coprime factorization unstable plant
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Source apportionment of PM_(2.5) using dispersion normalized positive matrix factorization(DN-PMF)in Beijing and Baoding,China
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作者 Ilhan Ryoo Taeyeon Kim +6 位作者 Jiwon Ryu Yeonseung Cheong Kwang-joo Moon Kwon-ho Jeon Philip K.Hopke Seung-Muk Yi Jieun Park 《Journal of Environmental Sciences》 2025年第9期395-408,共14页
Fine particulatematter(PM_(2.5))samples were collected in two neighboring cities,Beijing and Baoding,China.High-concentration events of PM_(2.5) in which the average mass concentration exceeded 75μg/m^(3) were freque... Fine particulatematter(PM_(2.5))samples were collected in two neighboring cities,Beijing and Baoding,China.High-concentration events of PM_(2.5) in which the average mass concentration exceeded 75μg/m^(3) were frequently observed during the heating season.Dispersion Normalized Positive Matrix Factorization was applied for the source apportionment of PM_(2.5) as minimize the dilution effects of meteorology and better reflect the source strengths in these two cities.Secondary nitrate had the highest contribution for Beijing(37.3%),and residential heating/biomass burning was the largest for Baoding(27.1%).Secondary nitrate,mobile,biomass burning,district heating,oil combustion,aged sea salt sources showed significant differences between the heating and non-heating seasons in Beijing for same period(2019.01.10–2019.08.22)(Mann-Whitney Rank Sum Test P<0.05).In case of Baoding,soil,residential heating/biomass burning,incinerator,coal combustion,oil combustion sources showed significant differences.The results of Pearson correlation analysis for the common sources between the two cities showed that long-range transported sources and some sources with seasonal patterns such as oil combustion and soil had high correlation coefficients.Conditional Bivariate Probability Function(CBPF)was used to identify the inflow directions for the sources,and joint-PSCF(Potential Source Contribution Function)was performed to determine the common potential source areas for sources affecting both cities.These models facilitated a more precise verification of city-specific influences on PM_(2.5) sources.The results of this study will aid in prioritizing air pollution mitigation strategies during the heating season and strengthening air quality management to reduce the impact of downwind neighboring cities. 展开更多
关键词 Source apportionment Dispersion normalized positive matrix factorization Adjacent cities Inter-city impact Source location Heating season Air quality management
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Latent-Factorization-of-Tensors-Incorporated Battery Cycle Life Prediction
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作者 Minzhi Chen Li Tao +1 位作者 Jungang Lou Xin Luo 《IEEE/CAA Journal of Automatica Sinica》 2025年第3期633-635,共3页
Dear Editor,This letter presents a latent-factorization-of-tensors(LFT)-incorporated battery cycle life prediction framework.Data-driven prognosis and health management(PHM)for battery pack(BP)can boost the safety and... Dear Editor,This letter presents a latent-factorization-of-tensors(LFT)-incorporated battery cycle life prediction framework.Data-driven prognosis and health management(PHM)for battery pack(BP)can boost the safety and sustainability of a battery management system(BMS),which relies heavily on the quality of the measured BP data like the voltage(V),current(I),and temperature(T). 展开更多
关键词 health management battery pack bp can latent factorization tensors battery cycle life prediction health management phm battery cycle battery pack battery management system bms which
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非酒精性脂肪性肝病病人血清长链非编码NTF3-5水平与胰岛素抵抗的关系 被引量:4
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作者 陈旋 《安徽医药》 CAS 2024年第2期285-289,共5页
目的 探讨长链非编码NTF3-5(Lnc-NTF3-5)在非酒精性脂肪性肝病(NAFLD)病人血清中的表达情况,并分析其与胰岛素抵抗的关系。方法 选取2019年12月至2020年12月来武汉市蔡甸区人民医院就诊并确诊为NAFLD病人115例为NAFLD组,根据疾病严重程... 目的 探讨长链非编码NTF3-5(Lnc-NTF3-5)在非酒精性脂肪性肝病(NAFLD)病人血清中的表达情况,并分析其与胰岛素抵抗的关系。方法 选取2019年12月至2020年12月来武汉市蔡甸区人民医院就诊并确诊为NAFLD病人115例为NAFLD组,根据疾病严重程度分为重度组(33例),中度组(40例),轻度组(42例)。同时选取55例健康者为健康对照组。收集一般资料,分别检测两组血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、谷氨酰基转移酶(GGT)、碱性磷酸酶(ALP),空腹血胰岛素(FINS),血糖(FPG)水平,根据FINS、FPG计算稳态模型胰岛素抵抗指数(HOMA-IR)及胰岛β细胞功能指数(HOMA-β);采用实时荧光定量PCR法对血清中Lnc-NTF3-5表达水平进行检测,Pearson法分析Lnc-NTF3-5表达水平与上述肝功能指标和糖脂代谢指标的相关性;受试者操作特征(ROC)曲线评价血清Lnc-NTF3-5、HOMA-IR对NAFLD发生的诊断价值。结果 NAFLD组血清中LncNTF3-5(1.59±0.37比1.01±0.23)、HOMA-IR(2.67±0.87比1.36±0.48)高于健康对照组,且NAFLD组Lnc-NTF3-5(2.02±0.43比1.53±0.37比1.32±0.31)、HOMA-IR(3.75±1.23比2.49±0.81比1.98±0.64)水平随着疾病严重程度逐渐升高,差异有统计学意义(F=67.41、66.02,P<0.05);NAFLD组TG[(3.23±1.02)mmol/L比(1.13±0.31)mmol/L]、TC[(5.15±0.88)mmol/L比(3.82±1.02)mmol/L]、LDL-C[(3.28±0.63)mmol/L比(2.65±0.31)mmol/L]、ALT[(55.33±18.34)U/L比(24.62±7.95)U/L]、GGT[(33.21±10.62)U/L比(26.94±8.32)U/L]、AST[(34.86±11.56)U/L比(22.32±4.26)U/L]、FPG[(5.63±1.02)mmol/L比(4.99±0.88)mmol/L]、FINS[(12.50±6.59)mU/L比(8.61±1.84)mU/L]、HOMA-β(4.92±0.80比4.61±0.67)均显著高于健康对照组,HDL-C[(1.03±0.45)mmol/L比(1.32±0.32)mmol/L]低于健康对照组,均差异有统计学意义(t=2.49~14.92,P<0.05);血清Lnc-NTF3-5和HOMA-IR均与HDL-C呈负相关(r=-0.54,P<0.05)(r=-0.61,P<0.05),而与TG、TC、LDL-C、ALT、GGT、AST、FPG、FINS、HOMA-β均呈正相关(r=0.23、0.22、0.23、0.21、0.53、0.19、0.54、0.55、0.52,P<0.05)(r=0.45、0.32、0.27、0.23、0.52、0.30、0.52、0.56、0.53,P<0.05);NAFLD病人血清中Lnc-NTF3-5表达水平和HOMA-IR呈正相关(r=0.37,P<0.001)。ROC结果显示,Lnc-NTF3-5、HOMA-IR预测NAFLD病人的曲线下面积(AUC)分别为[0.85,95%CI:(0.79,0.90);0.83,95%CI:(0.76,0.88)],对应的灵敏度分别为72.17%、61.74%,特异度分别为89.09%、98.18%,二者联合预测NAFLD病人的AUC为0.88,95%CI:(0.82,0.93),灵敏度为76.52%,特异度为96.36%。结论 NAFLD病人血清中Lnc-NTF3-5表达水平与HOMA-IR呈正相关,且二者均与病人肝功能和糖脂代谢有关,具有重要的临床意义。 展开更多
关键词 脂肪肝 非酒精性脂肪肝 长链非编码ntf3-5 胰岛素抵抗 相关性
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Health risk assessment of trace metal(loid)s in agricultural soils based on Monte Carlo simulation coupled with positive matrix factorization model in Chongqing, southwest China 被引量:4
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作者 MA Jie CHU Lijuan +3 位作者 SUN Jing WANG Shenglan GE Miao DENG Li 《Journal of Mountain Science》 SCIE CSCD 2024年第1期100-112,共13页
This study aimed to investigate the pollution characteristics, source apportionment, and health risks associated with trace metal(loid)s(TMs) in the major agricultural producing areas in Chongqing, China. We analyzed ... This study aimed to investigate the pollution characteristics, source apportionment, and health risks associated with trace metal(loid)s(TMs) in the major agricultural producing areas in Chongqing, China. We analyzed the source apportionment and assessed the health risk of TMs in agricultural soils by using positive matrix factorization(PMF) model and health risk assessment(HRA) model based on Monte Carlo simulation. Meanwhile, we combined PMF and HRA models to explore the health risks of TMs in agricultural soils by different pollution sources to determine the priority control factors. Results showed that the average contents of cadmium(Cd), arsenic (As), lead(Pb), chromium(Cr), copper(Cu), nickel(Ni), and zinc(Zn) in the soil were found to be 0.26, 5.93, 27.14, 61.32, 23.81, 32.45, and 78.65 mg/kg, respectively. Spatial analysis and source apportionment analysis revealed that urban and industrial sources, agricultural sources, and natural sources accounted for 33.0%, 27.7%, and 39.3% of TM accumulation in the soil, respectively. In the HRA model based on Monte Carlo simulation, noncarcinogenic risks were deemed negligible(hazard index <1), the carcinogenic risks were at acceptable level(10^(-6)<total carcinogenic risk ≤ 10^(-4)), with higher risks observed for children compared to adults. The relationship between TMs, their sources, and health risks indicated that urban and industrial sources were primarily associated with As, contributing to 75.1% of carcinogenic risks and 55.7% of non-carcinogenic risks, making them the primary control factors. Meanwhile, agricultural sources were primarily linked to Cd and Pb, contributing to 13.1% of carcinogenic risks and 21.8% of non-carcinogenic risks, designating them as secondary control factors. 展开更多
关键词 Monte Carlo simulation Health risk assessment Trace metal(loid)s Positive matrix factorization Agricultural soils
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Efficient Clustering Network Based on Matrix Factorization
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作者 Jieren Cheng Jimei Li +2 位作者 Faqiang Zeng Zhicong Tao and Yue Yang 《Computers, Materials & Continua》 SCIE EI 2024年第7期281-298,共18页
Contrastive learning is a significant research direction in the field of deep learning.However,existing data augmentation methods often lead to issues such as semantic drift in generated views while the complexity of ... Contrastive learning is a significant research direction in the field of deep learning.However,existing data augmentation methods often lead to issues such as semantic drift in generated views while the complexity of model pre-training limits further improvement in the performance of existing methods.To address these challenges,we propose the Efficient Clustering Network based on Matrix Factorization(ECN-MF).Specifically,we design a batched low-rank Singular Value Decomposition(SVD)algorithm for data augmentation to eliminate redundant information and uncover major patterns of variation and key information in the data.Additionally,we design a Mutual Information-Enhanced Clustering Module(MI-ECM)to accelerate the training process by leveraging a simple architecture to bring samples from the same cluster closer while pushing samples from other clusters apart.Extensive experiments on six datasets demonstrate that ECN-MF exhibits more effective performance compared to state-of-the-art algorithms. 展开更多
关键词 Contrastive learning CLUSTERING matrix factorization
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基于NTFS分区格式的U盘数据恢复的研究
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作者 乔楠 《计算机应用文摘》 2024年第2期121-122,125,共3页
U盘是一种非常普遍的数据存储设备,具有多种存储格式。其中,NTFS分区格式是最常见的,其存储和管理能力较强,但容易出现数据丢失的问题。因此,文章将采用WinHex软件详细介绍基于NTFS分区格式的U盘数据恢复方法,以确保U盘的正常运行。
关键词 U盘数据恢复 ntfS分区格式 数据存储 数据丢失
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NFA:A neural factorization autoencoder based online telephony fraud detection
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作者 Abdul Wahid Mounira Msahli +1 位作者 Albert Bifet Gerard Memmi 《Digital Communications and Networks》 SCIE CSCD 2024年第1期158-167,共10页
The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal ac... The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal activity on the network.To reduce these losses,a new fraud detection approach is required.Telecom fraud detection involves identifying a small number of fraudulent calls from a vast amount of call traffic.Developing an effective strategy to combat fraud has become challenging.Although much effort has been made to detect fraud,most existing methods are designed for batch processing,not real-time detection.To solve this problem,we propose an online fraud detection model using a Neural Factorization Autoencoder(NFA),which analyzes customer calling patterns to detect fraudulent calls.The model employs Neural Factorization Machines(NFM)and an Autoencoder(AE)to model calling patterns and a memory module to adapt to changing customer behaviour.We evaluate our approach on a large dataset of real-world call detail records and compare it with several state-of-the-art methods.Our results show that our approach outperforms the baselines,with an AUC of 91.06%,a TPR of 91.89%,an FPR of 14.76%,and an F1-score of 95.45%.These results demonstrate the effectiveness of our approach in detecting fraud in real-time and suggest that it can be a valuable tool for preventing fraud in telecommunications networks. 展开更多
关键词 Telecom industry Streaming anomaly detection Fraud analysis factorization machine Real-time system Security
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不同激活剂对富血小板血浆生长因子的影响 被引量:4
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作者 刘建香 冯星星 +3 位作者 王淑霞 周蓉 吕孟兴 屈柯暄 《中国组织工程研究》 CAS 北大核心 2025年第10期2067-2073,共7页
背景:生长因子是富血小板血浆在临床治疗中发挥作用的关键效应分子,不同激活剂激活富血小板血浆后生长因子浓度存在差异,是影响临床疗效的重要因素。目的:分析不同激活剂对富血小板血浆中生长因子质量浓度的影响。方法:招募12名健康志愿... 背景:生长因子是富血小板血浆在临床治疗中发挥作用的关键效应分子,不同激活剂激活富血小板血浆后生长因子浓度存在差异,是影响临床疗效的重要因素。目的:分析不同激活剂对富血小板血浆中生长因子质量浓度的影响。方法:招募12名健康志愿者,采集EDTA-K2抗凝静脉血,应用二次离心法制备富血小板血浆。比较静脉血与富血小板血浆中生长因子的质量浓度差异。将富血小板血浆分别与4种激活剂(生理盐水、凝血酶、葡萄糖酸钙、葡萄糖酸钙+凝血酶)按照体积比10∶1混匀,置于37℃恒温水浴箱孵育30 min,离心后提取上清液,检测生长因子质量浓度;利用血琼脂平板检测上清液中的细菌生长情况;采用Pearson相关分析不同激活剂与富血小板血浆中生长因子质量浓度的相关性,血小板计数值与富血小板血浆中生长因子质量浓度的相关性。结果与结论:(1)富血小板血浆中血小板衍生生长因子BB、血小板衍生生长因子AB、血管内皮生长因子、表皮生长因子的质量浓度分别是静脉血中对应生长因子质量浓度的8.7,22.2,2.3,2.8倍(P <0.05);(2)与生理盐水组比较,凝血酶组、葡萄糖酸钙组、葡萄糖酸钙+凝血酶组中血小板衍生生长因子BB、血小板衍生生长因子AB、血管内皮生长因子、表皮生长因子质量浓度升高(P <0.05);凝血酶组、葡萄糖酸钙组血小板衍生生长因子BB质量浓度高于葡萄糖酸钙+凝血酶组(P <0.05),凝血酶组血小板衍生生长因子AB质量浓度高于葡萄糖酸钙组、葡萄糖酸钙+凝血酶组(P <0.05),凝血酶组表皮生长因子质量浓度低于葡萄糖酸钙组、葡萄糖酸钙+凝血酶组(P <0.05);(3)血琼脂平板实验结果显示4组上清液中均无细菌生长;(4)Pearson相关分析显示,富血小板血浆中血小板衍生生长因子BB质量浓度与凝血酶存在正向强相关性(r=0.683,P <0.05),血管内皮生长因子质量浓度与凝血酶、葡萄糖酸钙、葡萄糖酸钙+凝血酶激混合剂存在正向强相关性(r=0.730,0.789,0.686,P <0.05);富血小板中血小板计数值与4种生长因子质量浓度均无相关性(P> 0.05);(5)结果提示,不同激活剂对富血小板血浆中生长因子浓度产生不同影响,建议临床根据不同生长因子质量浓度和治疗需求选择不同激活剂,以提高临床疗效。 展开更多
关键词 富血小板血浆 凝血酶 葡萄糖酸钙 血小板衍生生长因子BB 血小板衍生生长因子AB 血管内皮生长因子 表皮生长因子
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Symmetric Nonnegative Matrix Factorization for Vertex Centrality in Complex Networks
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作者 LU Pengli CHEN Wei +1 位作者 GUO Yuhong CHEN Yahong 《Journal of Shanghai Jiaotong university(Science)》 2024年第6期1037-1049,共13页
One of the most important problems in complex networks is to identify the influential vertices for understanding and controlling of information diffusion and disease spreading.Most of the current centrality algorithms... One of the most important problems in complex networks is to identify the influential vertices for understanding and controlling of information diffusion and disease spreading.Most of the current centrality algorithms focus on single feature or manually extract the attributes,which occasionally results in the failure to fully capture the vertex’s importance.A new vertex centrality approach based on symmetric nonnegative matrix factorization(SNMF),called VCSNMF,is proposed in this paper.For highlight the characteristics of a network,the adjacency matrix and the degree matrix are fused to represent original data of the network via a weighted linear combination.First,SNMF automatically extracts the latent characteristics of vertices by factorizing the established original data matrix.Then we prove that each vertex’s composite feature which is constructed with one-dimensional factor matrix can be approximated as the term of eigenvector associated with the spectral radius of the network,otherwise obtained by the factor matrix on the hyperspace.Finally,VCSNMF integrates the composite feature and the topological structure to evaluate the performance of vertices.To verify the effectiveness of the VCSNMF criterion,eight existing centrality approaches are used as comparison measures to rank influential vertices in ten real-world networks.The experimental results assert the superiority of the method. 展开更多
关键词 complex networks CENTRALITY symmetric nonnegative matrix factorization(SNMF)
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妊娠晚期缺铁性贫血的危险因素和列线图预测模型 被引量:3
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作者 杨林 王亦雄 +1 位作者 潘辉 徐扬 《中山大学学报(医学科学版)》 北大核心 2025年第1期116-122,共7页
【目的】对孕妇妊娠期缺铁性贫血(IDA)的相关因素进行分析,根据其独立的危险因素,构建预测妊娠晚期缺铁性贫血的列线图预测模型,以期获得IDA的有效干预方案。【方法】将2022年7月—2023年12月在扬州市妇幼保健院定期产检的孕妇为调查对... 【目的】对孕妇妊娠期缺铁性贫血(IDA)的相关因素进行分析,根据其独立的危险因素,构建预测妊娠晚期缺铁性贫血的列线图预测模型,以期获得IDA的有效干预方案。【方法】将2022年7月—2023年12月在扬州市妇幼保健院定期产检的孕妇为调查对象,采取统一调查问卷结合监测血常规的方法跟踪孕妇直至分娩,入组500例,最终完成482例。根据孕晚期有无IDA分为IDA组与非IDA组。对可能影响缺铁性贫血的危险因素进行单因素分析及多因素logistic回归分析,并采用R软件建立预测发生的列线图模型。【结果】482例妊娠晚期女性发生IDA有96例,发生率为19.92%;单因素分析显示,经济独立情况、孕前体质指数、孕次及孕期有无正规补铁与妊娠晚期IDA的发生有关,差异均有统计学意义(P<0.05)。多因素Logistic回归分析显示,经济独立(P=0.031,OR=0.583)、孕期正规补铁(P<0.001,OR=5.337)是IDA的保护因素;孕前低体质指数(P=0.021,OR=2.375),孕次≥3次(P=0.015,OR=2.253)是IDA的危险因素。ROC曲线显示,列线图模型预测IDA发生的曲线下面积为0.84,最佳截断值为-1.481,预测灵敏度为81.2%,特异度分别为75.1%。【结论】妊娠晚期IDA发病率较高;孕前低体质指数、孕次大于3次是妊娠晚期IDA的危险因素;经济独立、正规补铁是妊娠晚期IDA的保护因素。可利用上述因素构建预测模型、采取预防性措施研究降低妊娠晚期IDA发病率的可行性方案。 展开更多
关键词 妊娠晚期 缺铁性贫血 危险因素 保护因素 列线图 预测模型
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乌兰布和沙漠沙冬青液流变化及其影响因子 被引量:1
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作者 董雪 黄雅茹 +5 位作者 李帅 徐光甫 陈晓娜 刘源 郭俊廷 辛智鸣 《中国农业科技导报(中英文)》 北大核心 2025年第7期241-249,共9页
为揭示乌兰布和沙漠沙冬青耗水特征,研究沙冬青晴天和雨天液流变化规律及其影响因素,采用热扩散树干液流仪监测沙冬青液流速率日变化,并利用自动气象站同步监测空气温度、空气相对湿度等气象因子日变化,利用土壤温湿度自动监测仪测定土... 为揭示乌兰布和沙漠沙冬青耗水特征,研究沙冬青晴天和雨天液流变化规律及其影响因素,采用热扩散树干液流仪监测沙冬青液流速率日变化,并利用自动气象站同步监测空气温度、空气相对湿度等气象因子日变化,利用土壤温湿度自动监测仪测定土壤因子日变化,研究不同直径沙冬青液流在不同天气条件下的日变化特征及其与环境因子的关系。结果表明,沙冬青白天液流较高,夜晚存在液流,但液流速率小且变化不大。沙冬青直径越大,日均液流速率也越大;晴天与雨天沙冬青液流变化曲线分别呈“单峰型”与“双峰型”,晴天液流速率日均值高于雨天。晴天和雨天条件下,沙冬青液流速率与太阳辐射、土壤温度、空气温度、饱和水气压差呈显著正相关,与空气相对湿度呈显著负相关,与土壤含水量相关性不显著。回归方程分析表明,对沙冬青晴天液流速率影响最大的是太阳辐射,可单独解释液流变化的89.1%,对雨天液流速率影响最大的是土壤温度,可单独解释液流变化的85.8%。综上可知,乌兰布和沙漠沙冬青液流速率在晴天与雨天的变化趋势存在差异,晴天沙冬青液流速率主要受太阳辐射影响,雨天主要受土壤温度影响,晴天和雨天液流速率的变化与太阳辐射、土壤温度、空气温度、饱和水汽压亏缺呈正相关,与相对湿度呈负相关,与土壤含水量相关性不显著。研究结果对于明确乌兰布和沙漠沙冬青耗水规律具有重要意义,对生物多样性保育和区域植被建设具有一定理论意义。 展开更多
关键词 沙冬青 液流 乌兰布和沙漠 气象因子 土壤因子
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ATF3通过NF-κB信号通路调控动脉粥样硬化斑块内的炎症反应 被引量:2
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作者 夏冰 彭进 +6 位作者 丁九阳 王杰 唐国伟 刘国杰 王沄 万昌武 乐翠云 《南方医科大学学报》 北大核心 2025年第6期1131-1142,共12页
目的 探讨转录激活因子3(ATF3)在动脉粥样硬化斑块内的表达及其调控炎症反应参与动脉粥样硬化(AS)进程的机制。方法 收集尸检案例中的人冠状动脉标本,免疫荧光和Western blotting检测ATF3蛋白表达及分布;高脂饮食12周后的载脂蛋白E基因... 目的 探讨转录激活因子3(ATF3)在动脉粥样硬化斑块内的表达及其调控炎症反应参与动脉粥样硬化(AS)进程的机制。方法 收集尸检案例中的人冠状动脉标本,免疫荧光和Western blotting检测ATF3蛋白表达及分布;高脂饮食12周后的载脂蛋白E基因敲除(ApoE-/-)小鼠尾静脉注射9型腺相关病毒(AAV9)敲低ATF3的表达,继续高脂喂养5周构建ATF3基因敲除的动脉粥样硬化ApoE-/-小鼠模型。麻醉处死后观察主动脉斑块结构改变,检测斑块内ATF3、炎症相关因子及NF-κB信号通路蛋白表达改变,并在体外构建ATF3的过表达质粒及siRNA干预THP-1诱导的泡沫细胞模型验证ATF3与NF-κB信号通路间关系。结果 在人冠状动脉粥样硬化斑块内,ATF3的表达增高(P<0.05),且与CD68呈部分共表达;在ATF3基因敲除后,小鼠的主动脉斑块体积增大(P<0.05),斑块内炎症相关因子(CD45、CD68、IL-1β、TNF-α)表达增强(P<0.05),NF-κB信号通路相关蛋白(PIKKα/β、P-NF-κB p65)表达增高(P<0.05),VCAM1、MMP9及MMP2表达增强(P<0.05);在离体THP-1细胞实验中验证了沉默ATF3后NF-κB信号通路进一步激活,而过表达ATF3后NF-κB信号受到抑制。结论 动脉粥样硬化诱导ATF3的表达,ATF3的缺乏会促进AS的发生,增强斑块内炎症反应,其机制可能是通过进一步激活NF-κB信号通路导致,ATF3可能是AS潜在的治疗靶点。 展开更多
关键词 动脉粥样硬化 转录激活因子3 炎症反应 NF-ΚB 冠心病猝死
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数字经济与农业全要素生产率:技术进步和要素流动的视角 被引量:10
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作者 龚勤林 乔涛 冷玉婷 《华中农业大学学报(社会科学版)》 北大核心 2025年第2期15-27,共13页
在持续提高农业创新力、竞争力和全要素生产率的背景下,利用2011−2019年中国273个地级市面板数据,实证检验了数字经济对农业全要素生产率的影响。研究结论如下:第一,数字经济发展能够显著提升农业全要素生产率;第二,技术进步和要素流动... 在持续提高农业创新力、竞争力和全要素生产率的背景下,利用2011−2019年中国273个地级市面板数据,实证检验了数字经济对农业全要素生产率的影响。研究结论如下:第一,数字经济发展能够显著提升农业全要素生产率;第二,技术进步和要素流动是数字经济影响农业全要素生产率的作用机制,具体包括农业技术进步、农业劳动力转移、农用耕地流转与农业资本深化;第三,数字经济作用存在异质性,其对我国西部地区农业全要素生产率提升作用不显著,且由于粮食主产区的萎缩与限制政策,数字经济带来的农业劳动力转移与农业资本深化效应会受到抑制。基于此,文章认为政府应当推动农业基础设施数字化、助力降低数字技术使用门槛、以数字赋能农业科技创新与应用、健全农地流转管理体制,强化粮食主产区农业支持政策等。 展开更多
关键词 数字经济 技术进步 要素流动 农业全要素生产率
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某大型三甲医院门诊患者满意度调查及影响因素分析 被引量:7
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作者 房洪军 王浩 +3 位作者 李晶 杨宏艳 关思楠 李航 《中国医院管理》 北大核心 2025年第2期40-45,共6页
目的 分析某大型三甲公立医院多院区门诊患者就医体验满意度。方法 2024年1—3月,基于问卷星平台向北京大学第一医院门诊当日就诊患者推送门诊就诊满意度调查问卷,分析门诊患者对医疗服务的满意率和满意度评分,并采用logistic回归分析... 目的 分析某大型三甲公立医院多院区门诊患者就医体验满意度。方法 2024年1—3月,基于问卷星平台向北京大学第一医院门诊当日就诊患者推送门诊就诊满意度调查问卷,分析门诊患者对医疗服务的满意率和满意度评分,并采用logistic回归分析探索门诊患者就诊满意度的影响因素。结果 64.0%认为非常满意,26.1%认为比较满意;候诊时长和就诊时长发挥重要影响作用;医生的疾病诊断、治疗和预后、健康宣教和随诊安排可能会影响门诊患者就诊体验;门诊缴费、门诊环境、门诊患者感知抱怨及不满的及时解决都影响门诊患者就诊满意度。结论 门诊患者整体满意度较高,多院区门诊医疗服务患者满意度保持一致。医院应在保证门诊医疗质量的前提下,积极进行个体化健康教育,优化就医流程,延伸门诊服务,进一步提升门诊患者就医获得感。 展开更多
关键词 三甲医院 门诊患者 满意度 影响因素
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