Objectives:Glioma,as the most lethal primary brain malignancy with poor prognosis,requires further elucidation on the functional role of long noncoding RNA(lncRNA)DDX11 antisense RNA 1(DDX11-AS1)in its pathogenesis,de...Objectives:Glioma,as the most lethal primary brain malignancy with poor prognosis,requires further elucidation on the functional role of long noncoding RNA(lncRNA)DDX11 antisense RNA 1(DDX11-AS1)in its pathogenesis,despite its established oncogenic functions in other cancers.Therefore,this study sought to characterize the oncogenic role and molecular mechanism of DDX11-AS1 in glioma.Methods:DDX11-AS1 expression levels were analyzed in clinical surgical glioma specimens and publicly available datasets.The functional roles of DDX11-AS1 on glioma cell proliferation and migration were investigated using in vitro knockdown and overexpression assays.In vivo tumor growth was assessed using orthotopic glioma-bearing mouse models.To elucidate the regulatory axis involving DDX11-AS1,miR-1183,and E2F transcription factor 7(E2F7),we performed competitive endogenous RNA(ceRNA)analysis and conducted functional rescue experiments via miR-1183 inhibition.Results:DDX11-AS1 expression was markedly upregulated in clinical glioma specimens.Functionally,DDX11-AS1 knockdown significantly suppressed glioma cell proliferation and migration in vitro,while its overexpression exacerbated these malignant phenotypes.Orthotopic glioma-bearingmouse models confirmed that DDX11-AS1 drives in vivo glioma tumor growth.Mechanistically,DDX11-AS1 functions as a ceRNA by competitively interacting with miR-1183.Critically,inhibition of miR-1183 rescued the suppressive effects of DDX11-AS1 knockdown on glioma tumorigenic phenotypes and restored E2F7 expression levels.Conclusions:This study demonstrates that lncRNA DDX11-AS1 promotes glioma progression by regulating the miR-1183/E2F7 axis,indicating a potential therapeutic target for glioma.展开更多
目前在电力行业中广泛应用的SF6气体具有严重的温室效应,寻找一种绝缘性能优良的环保型替代气体成为研究热点。近年来,C3F7CN及其混合气体凭借出色的绝缘性能和环保特性得到了国内外学者的广泛关注。目前针对其放电分解机理的研究较少...目前在电力行业中广泛应用的SF6气体具有严重的温室效应,寻找一种绝缘性能优良的环保型替代气体成为研究热点。近年来,C3F7CN及其混合气体凭借出色的绝缘性能和环保特性得到了国内外学者的广泛关注。目前针对其放电分解机理的研究较少。首先,基于ReaxFF反应分子动力学方法和密度泛函理论,从微观层面模拟研究了C3F7CN/CO2混合气体的分解机理。基于构建的C3F7CN/CO2混合气体模型,研究了温度对C3F7CN分解过程的影响,分析了其可能的分解路径、产物类型及分布等。计算结果表明,C3F7CN在局部过热或放电等故障条件下分解容易产生CF3·、C3F7·、C·、CF3CFCN·、CF2·和F·等各类自由基,上述自由基复合将产生CF4、C2F6、C3F8、CF3CN、CO等产物。其次,利用气体绝缘试验平台和气相色谱质谱联用仪(gas chromatography mass spectrometer,GC-MS),试验分析了其多次击穿后的分解产物。试验结果表明,混合气体多次击穿后的主要分解产物是CF4、C2F6、CF3CN。这些产物分子均具有比较好的绝缘能力,保障了C3F7CN/CO2混合气体的绝缘性能不被破环。相关结论为进一步探究C3F7CN/CO2混合气体的绝缘特性及协同效应等课题提供理论依据和工程指导。展开更多
基金supported by Shenzhen Science and Technology Program(JCYJ20220530152614033,JCYJ20230807142213027)Funding Statement Special Fund for Economic and Technological Development in Longgang District,Shenzhen(LGKCYLWS2023028).
文摘Objectives:Glioma,as the most lethal primary brain malignancy with poor prognosis,requires further elucidation on the functional role of long noncoding RNA(lncRNA)DDX11 antisense RNA 1(DDX11-AS1)in its pathogenesis,despite its established oncogenic functions in other cancers.Therefore,this study sought to characterize the oncogenic role and molecular mechanism of DDX11-AS1 in glioma.Methods:DDX11-AS1 expression levels were analyzed in clinical surgical glioma specimens and publicly available datasets.The functional roles of DDX11-AS1 on glioma cell proliferation and migration were investigated using in vitro knockdown and overexpression assays.In vivo tumor growth was assessed using orthotopic glioma-bearing mouse models.To elucidate the regulatory axis involving DDX11-AS1,miR-1183,and E2F transcription factor 7(E2F7),we performed competitive endogenous RNA(ceRNA)analysis and conducted functional rescue experiments via miR-1183 inhibition.Results:DDX11-AS1 expression was markedly upregulated in clinical glioma specimens.Functionally,DDX11-AS1 knockdown significantly suppressed glioma cell proliferation and migration in vitro,while its overexpression exacerbated these malignant phenotypes.Orthotopic glioma-bearingmouse models confirmed that DDX11-AS1 drives in vivo glioma tumor growth.Mechanistically,DDX11-AS1 functions as a ceRNA by competitively interacting with miR-1183.Critically,inhibition of miR-1183 rescued the suppressive effects of DDX11-AS1 knockdown on glioma tumorigenic phenotypes and restored E2F7 expression levels.Conclusions:This study demonstrates that lncRNA DDX11-AS1 promotes glioma progression by regulating the miR-1183/E2F7 axis,indicating a potential therapeutic target for glioma.
文摘目前在电力行业中广泛应用的SF6气体具有严重的温室效应,寻找一种绝缘性能优良的环保型替代气体成为研究热点。近年来,C3F7CN及其混合气体凭借出色的绝缘性能和环保特性得到了国内外学者的广泛关注。目前针对其放电分解机理的研究较少。首先,基于ReaxFF反应分子动力学方法和密度泛函理论,从微观层面模拟研究了C3F7CN/CO2混合气体的分解机理。基于构建的C3F7CN/CO2混合气体模型,研究了温度对C3F7CN分解过程的影响,分析了其可能的分解路径、产物类型及分布等。计算结果表明,C3F7CN在局部过热或放电等故障条件下分解容易产生CF3·、C3F7·、C·、CF3CFCN·、CF2·和F·等各类自由基,上述自由基复合将产生CF4、C2F6、C3F8、CF3CN、CO等产物。其次,利用气体绝缘试验平台和气相色谱质谱联用仪(gas chromatography mass spectrometer,GC-MS),试验分析了其多次击穿后的分解产物。试验结果表明,混合气体多次击穿后的主要分解产物是CF4、C2F6、CF3CN。这些产物分子均具有比较好的绝缘能力,保障了C3F7CN/CO2混合气体的绝缘性能不被破环。相关结论为进一步探究C3F7CN/CO2混合气体的绝缘特性及协同效应等课题提供理论依据和工程指导。
