The Chinese medicine Extractum trametes robiniophila murr(ETRM)is the extract of a type of fungus.Recent studies have suggested that ETRM efficiently improves the effacement of podocyte foot processes in adriamycin(AD...The Chinese medicine Extractum trametes robiniophila murr(ETRM)is the extract of a type of fungus.Recent studies have suggested that ETRM efficiently improves the effacement of podocyte foot processes in adriamycin(ADR)-induced nephrotic rats.In the present study,we aimed to assess whether ETRM modulated the actin rearrangements of podocytes and involved signaling molecules,includingα-actinin-4 and nephrin.Podocytes were treated with ADR(0.5μmol/L),ADR(0.5μmol/L)+dexamethasone(Dex)(1μmol/L),ADR(0.5μmol/L)+ETRM(10 mg/mL).The F-actin in the podocytes was stained by fluorescent phallotoxins and observed by confocal microscopy.The expression levels ofα-actinin-4 and nephrin were tested by real-time PCR and Western blotting analysis.The administration of ETRM could significantly prevent ADR-treated podocytes from actin rearrangement.Both ETRM and Dex could stabilize podocyte actin cytoskeletons.Moreover,α-actinin-4 might act as a potential target for ETRM functionality in podocyte actin rearrangements.However,pretreatment with ETRM could not inhibit the up-regulation of nephrin as a result of ADR treatment.ETRM could improve the cytoskeleton stability in ADR-induced actin rearrangement of podocytes via regulatingα-actinin-4 expression.展开更多
In this work,xanthophylls were extracted from Marigold using supercritical CO_(2).A response surface experimental design was used to optimize operating conditions for extraction pressure(22-30 MPa),extraction temperat...In this work,xanthophylls were extracted from Marigold using supercritical CO_(2).A response surface experimental design was used to optimize operating conditions for extraction pressure(22-30 MPa),extraction temperature(50-60℃),CO_(2) flow rate(9-15 kg/h).The maximum concentration of xanthophyll in extracts was determined to be 35.8%by high performance liquid chromatography(HPLC),and optimum xanthophylls extraction was obtained at 55℃,26 MPa and 12 kg/h.The optimization results demonstrated that for xanthophylls extraction,all variables(temperature,pressure and CO_(2) flow rate)were to be the influential variables,with the statistical significant effect p-value was smaller than 0.05.展开更多
采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取...采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。展开更多
基金The National Natural Science Foundation of China(Grant No.81660130)the Natural Science Foundation of Gansu Province,China(Grant No.18JR3RA045).
文摘The Chinese medicine Extractum trametes robiniophila murr(ETRM)is the extract of a type of fungus.Recent studies have suggested that ETRM efficiently improves the effacement of podocyte foot processes in adriamycin(ADR)-induced nephrotic rats.In the present study,we aimed to assess whether ETRM modulated the actin rearrangements of podocytes and involved signaling molecules,includingα-actinin-4 and nephrin.Podocytes were treated with ADR(0.5μmol/L),ADR(0.5μmol/L)+dexamethasone(Dex)(1μmol/L),ADR(0.5μmol/L)+ETRM(10 mg/mL).The F-actin in the podocytes was stained by fluorescent phallotoxins and observed by confocal microscopy.The expression levels ofα-actinin-4 and nephrin were tested by real-time PCR and Western blotting analysis.The administration of ETRM could significantly prevent ADR-treated podocytes from actin rearrangement.Both ETRM and Dex could stabilize podocyte actin cytoskeletons.Moreover,α-actinin-4 might act as a potential target for ETRM functionality in podocyte actin rearrangements.However,pretreatment with ETRM could not inhibit the up-regulation of nephrin as a result of ADR treatment.ETRM could improve the cytoskeleton stability in ADR-induced actin rearrangement of podocytes via regulatingα-actinin-4 expression.
基金supported by Shandong Provincial Education Department Plan for Scientific and Technology Development(J08LF05)。
文摘In this work,xanthophylls were extracted from Marigold using supercritical CO_(2).A response surface experimental design was used to optimize operating conditions for extraction pressure(22-30 MPa),extraction temperature(50-60℃),CO_(2) flow rate(9-15 kg/h).The maximum concentration of xanthophyll in extracts was determined to be 35.8%by high performance liquid chromatography(HPLC),and optimum xanthophylls extraction was obtained at 55℃,26 MPa and 12 kg/h.The optimization results demonstrated that for xanthophylls extraction,all variables(temperature,pressure and CO_(2) flow rate)were to be the influential variables,with the statistical significant effect p-value was smaller than 0.05.
文摘采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。
